Cited by 260RNA and protein synthesis mechanismsRNA Research and SplicingPI3K/AKT/mTOR signaling in cancer

IRES-mediated cap-independent translation, a path leading to hidden proteome

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Yang, Yun, Wang, Zefeng·2019·Journal of Molecular Cell Biology

Abstract

Most eukaryotic mRNAs are translated in a cap-dependent fashion; however, under stress conditions, the cap-independent translation driven by internal ribosomal entry sites (IRESs) can serve as an alternative mechanism for protein production. Many IRESs have been discovered from viral or cellular mRNAs to promote ribosome assembly and initiate translation by recruiting different trans-acting factors. Although the mechanisms of translation initiation driven by viral IRESs are relatively well understood, the existence of cellular IRESs is still under debate due to the limitations of translation reporter systems used to assay IRES activities. A recent screen identified > 1000 putative IRESs from viral and human mRNAs, expanding the scope and mechanism for cap-independent translation. Additionally, a large number of circular RNAs lacking free ends were identified in eukaryotic cells, many of which are found to be translated through IRESs. These findings suggest that IRESs may play a previously unappreciated role in driving translation of the new type of mRNA, implying a hidden proteome produced from cap-independent translation.

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Authors
Yang, Y., & Wang, Z.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
  • Yang, Yun
  • Wang, Zefeng
Venue
Journal of Molecular Cell Biology
Published
2019-09-03
Volume
11
Issue
10
Pages
911-919
ISSN
  • 1674-2788 (ISSN-L)
  • 1759-4685
OpenAlex
W2971386099
Cited by
260
References
80
Citations by year
2026: 30 · 2025: 45 · 2024: 53 · 2023: 39 · 2022: 43 · 2021: 32 · 2020: 17 · 2019: 1
FWCI
2.05
Type
journal article
Language
en
Open access
Locations
  • Journal of Molecular Cell Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Youth Innovation Promotion Association CAS and Shanghai Science and Technology Committee Rising-Star Program (19QA1410500)
  • CAS Pioneer 100-Talent Program
  • National Natural Science Foundation of China (31870814, 91753135, 31730110, 31661143031, 31570823)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
Keywords
internal ribosome entry site, cap-independent translation, circular RNA, translation initiation, trans-acting factors
MeSH terms
Internal Ribosome Entry Sites, Humans, Models, Biological, Ribosomes, Protein Biosynthesis, Proteome

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