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Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database

Open access
Winsor, Geoffrey L., Griffiths, Emma J., Lo, Raymond, Dhillon, Bhavjinder K., Shay, Julie A., Brinkman, Fiona S. L.·2016·Nucleic Acids Research

Abstract

The Pseudomonas Genome Database (http://www.pseudomonas.com) is well known for the application of community-based annotation approaches for producing a high-quality Pseudomonas aeruginosa PAO1 genome annotation, and facilitating whole-genome comparative analyses with other Pseudomonas strains. To aid analysis of potentially thousands of complete and draft genome assemblies, this database and analysis platform was upgraded to integrate curated genome annotations and isolate metadata with enhanced tools for larger scale comparative analysis and visualization. Manually curated gene annotations are supplemented with improved computational analyses that help identify putative drug targets and vaccine candidates or assist with evolutionary studies by identifying orthologs, pathogen-associated genes and genomic islands. The database schema has been updated to integrate isolate metadata that will facilitate more powerful analysis of genomes across datasets in the future. We continue to place an emphasis on providing high-quality updates to gene annotations through regular review of the scientific literature and using community-based approaches including a major new Pseudomonas community initiative for the assignment of high-quality gene ontology terms to genes. As we further expand from thousands of genomes, we plan to provide enhancements that will aid data visualization and analysis arising from whole-genome comparative studies including more pan-genome and population-based approaches.

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Metadata

Authors
Winsor, G. L., Griffiths, E. J., Lo, R., Dhillon, B. K., Shay, J. A., & Brinkman, F. S. L.
Venue
Nucleic Acids Research
Published
2015-11-17
Volume
44
Issue
D1
Pages
D646-D653
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2179324586
Cited by
1,050
References
1
FWCI
24.57
Type
journal article
Language
en
Open access
gold

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