Cited by 70Microbial Metabolic Engineering and BioproductionBacterial Genetics and BiotechnologyGene Regulatory Network Analysis

Engineered Transcriptional Systems for Cyanobacterial Biotechnology

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Camsund, Daniel, Lindblad, Peter·2014·Frontiers in Bioengineering and Biotechnology

Abstract

Cyanobacteria can function as solar-driven biofactories thanks to their ability to perform photosynthesis and the ease with which they are genetically modified. In this review, we discuss transcriptional parts and promoters available for engineering cyanobacteria. First, we go through special cyanobacterial characteristics that may impact engineering, including the unusual cyanobacterial RNA polymerase, sigma factors and promoter types, mRNA stability, circadian rhythm, and gene dosage effects. Then, we continue with discussing component characteristics that are desirable for synthetic biology approaches, including decoupling, modularity, and orthogonality. We then summarize and discuss the latest promoters for use in cyanobacteria regarding characteristics such as regulation, strength, and dynamic range and suggest potential uses. Finally, we provide an outlook and suggest future developments that would advance the field and accelerate the use of cyanobacteria for renewable biotechnology.

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Authors
Camsund, D., & Lindblad, P.
Affiliations
Uppsala University (SE)
Corresponding author
Lindblad, Peter
Venue
Frontiers in Bioengineering and Biotechnology
Published
2014-10-01
Volume
2
Pages
40-40
ISSN
2296-4185 (ISSN-L)
OpenAlex
W2051792223
Cited by
70
References
100
Citations by year
2026: 4 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 8 · 2020: 5 · 2019: 8 · 2018: 10 · 2017: 5 · 2016: 9 · 2015: 8
FWCI
0.69
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • European Commission (2007–2013, 308518, FP7 2007-2013, FP7/2007)
  • Knut och Alice Wallenbergs Stiftelse
  • Energimyndigheten
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords
cyanobacteria, transcriptional regulation, promoter, synthetic biology, sigma factor, mRNA stability, circadian rhythm, gene dosage effect, decoupling, modularity, orthogonality, genetic engineering

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