Engineered Transcriptional Systems for Cyanobacterial Biotechnology
Open accessAbstract
Cyanobacteria can function as solar-driven biofactories thanks to their ability to perform photosynthesis and the ease with which they are genetically modified. In this review, we discuss transcriptional parts and promoters available for engineering cyanobacteria. First, we go through special cyanobacterial characteristics that may impact engineering, including the unusual cyanobacterial RNA polymerase, sigma factors and promoter types, mRNA stability, circadian rhythm, and gene dosage effects. Then, we continue with discussing component characteristics that are desirable for synthetic biology approaches, including decoupling, modularity, and orthogonality. We then summarize and discuss the latest promoters for use in cyanobacteria regarding characteristics such as regulation, strength, and dynamic range and suggest potential uses. Finally, we provide an outlook and suggest future developments that would advance the field and accelerate the use of cyanobacteria for renewable biotechnology.
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Both native and synthetic promoters have been employed to modulate gene expression within cyanobacteria.
“Both native and synthetic promoters have been employed to modulate gene expression within these organisms [97].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [97]
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- Authors
- Camsund, D., & Lindblad, P.
- Affiliations
- Uppsala University (SE)
- Corresponding author
- Lindblad, Peter
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2014-10-01
- Volume
- 2
- Pages
- 40-40
- ISSN
- 2296-4185 (ISSN-L)
- PMC
- PMC4181335
- OpenAlex
- W2051792223
- Cited by
- 70
- References
- 100
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 8 · 2020: 5 · 2019: 8 · 2018: 10 · 2017: 5 · 2016: 9 · 2015: 8
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- European Commission (2007–2013, 308518, FP7 2007-2013, FP7/2007)
- Knut och Alice Wallenbergs Stiftelse
- Energimyndigheten
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- cyanobacteria, transcriptional regulation, promoter, synthetic biology, sigma factor, mRNA stability, circadian rhythm, gene dosage effect, decoupling, modularity, orthogonality, genetic engineering
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