Developmental Dynamics of the Definitive Mouse Placenta Assessed by Stereology1
Abstract
The mouse is an excellent model for studying the genetic basis of placental development, but analyses are restricted by the lack of quantitative data describing normal murine placental structure. This study establishes a technique for generating such data, applies stereological techniques on systematic uniform random sections of placentas between E12.5-E18.5 of gestation (E1.0 = day of the vaginal plug), and considers the results in the context of development of the labyrinth zone. Half of each placenta was wax embedded and exhaustively sectioned to determine absolute volumes of the labyrinth zone (Lz), junctional zone (Jz), and decidua using the Cavalieri principle. The other half was resin embedded and 1-microm sections were used to generate all volume, surface, and length densities within the Lz. Maximum placental volume is reached by E16.5, whereas the Lz volume fraction increases until E18.5 at the expense of the Jz and decidua. Within the Lz, the absolute volume and surface area of maternal blood spaces (MBS) expand rapidly between E14.5 and E16.5, with no increase thereafter. In contrast, fetal capillary development is linear and continues for longer than that of the MBS. The interhemal membrane separating maternal and fetal circulations undergoes thinning prior to expansion of maternal and fetal surface areas, achieving a harmonic mean thickness of 4.39 microm by E18.5. The specific diffusion capacity for oxygen of the interhemal membrane is maximal by E16.5, which may be necessary to support rapid fetal growth until the end of gestation.
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Total fetal capillary surface area increases, favoring exchange, although mean capillary diameter is reduced.
“Consequently, total fetal capillary surface area increases, favoring exchange, although mean diameter is reduced, as in the mouse (54,116).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [116]
The mouse placenta undergoes rapid growth, with weight reaching a maximum around E16.5 and plateauing or declining thereafter.
“The placenta undergoes rapid growth, with weight reaching a maximum around E16.5 and plateauing, or even declining, thereafter (116).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [116]
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- Authors
- Coan, P. M., Ferguson-Smith, A. C., & Burton, G. J.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- Burton, Graham J.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-06-01
- Volume
- 70
- Issue
- 6
- Pages
- 1806-1813
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W2152460775
- Cited by
- 296
- References
- 37
- Citations by year
- 2026: 12 · 2025: 10 · 2024: 12 · 2023: 14 · 2022: 9 · 2021: 25 · 2020: 21 · 2019: 11 · 2018: 16 · 2017: 14 · 2016: 12 · 2015: 20 · 2014: 9 · 2013: 18 · 2012: 25
- FWCI
- 13.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biology of Reproduction (publishedVersion)
- PubMed (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Funders
- Anatomical Society
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
- Keywords
- placental development, mouse placenta, labyrinth zone, junctional zone, decidua, oxygen diffusion capacity, stereology, Cavalieri's principle, placental volume, gestational age
- MeSH terms
- Animals, Diffusion, Female, Fetus, Gestational Age, Mice, Inbred C57BL, Oxygen, Placenta, Placentation, Pregnancy, Fetal Development, Mice
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