Cited by 56Adipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic DiseasesMesenchymal stem cell research

De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue

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Gavin, Kathleen M., Gutman, Jonathan A., Kohrt, Wendy M., Wei, Qi, Shea, Karen L., Miller, Heidi L., Sullivan, Timothy M., Erickson, Paul F. +8 more·2016·The FASEB Journal

Abstract

White adipocytes in adults are typically derived from tissue resident mesenchymal progenitors. The recent identification of de novo production of adipocytes from bone marrow progenitor‐derived cells in mice challenges this paradigm and indicates an alternative lineage specification that adipocytes exist. We hypothesized that alternative lineage specification of white adipocytes is also present in human adipose tissue. Bone marrow from transgenic mice in which luciferase expression is governed by the adipocyte‐restricted adiponectin gene promoter was adoptively transferred to wild‐type recipient mice. Light emission was quantitated in recipients by in vivo imaging and direct enzyme assay. Adipocytes were also obtained from human recipients of hematopoietic stem cell transplantation. DNA was isolated, and microsatellite polymorphisms were exploited to quantify donor/recipient chimerism. Luciferase emission was detected from major fat depots of transplanted mice. No light emission was observed from intestines, liver, or lungs. Up to 35% of adipocytes in humans were generated from donor marrow cells in the absence of cell fusion. Nontransplanted mice and stromal‐vascular fraction samples were used as negative and positive controls for the mouse and human experiments, respectively. This study provides evidence for a nontissue resident origin of an adipocyte subpopulation in both mice and humans.—Gavin, K. M., Gutman, J. A., Kohrt, W. M., Wei, Q., Shea, K. L., Miller, H. L., Sullivan, T. M., Erickson, P. F., Helm, K. M., Acosta, A. S., Childs, C. R., Musselwhite, E., Varella‐Garcia, M., Kelly, K., Majka, S. M., Klemm, D. J., De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue. FASEB J. 30, 1096–1108 (2016). www.fasebj.org

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Authors
Gavin, K. M., Gutman, J. A., Kohrt, W. M., Wei, Q., Shea, K. L., Miller, H. L., Sullivan, T. M., Erickson, P. F., Helm, K. M., Acosta, A. S., Childs, C. R., Musselwhite, E., Varella‐Garcia, M., Kelly, K., Majka, S. M., & Klemm, D. J.
Affiliations
  • University of Colorado Anschutz (US)
  • Children's Hospital Colorado (US)
  • University of Colorado Cancer Center (US)
  • Vanderbilt University (US)
Corresponding author
Klemm, Dwight J.
Venue
The FASEB Journal
Publisher
Wiley
Published
2015-11-18
Volume
30
Issue
3
Pages
1096-1108
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2177636187
Cited by
55
References
69
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 9 · 2020: 3 · 2019: 4 · 2018: 9 · 2017: 9 · 2016: 6
FWCI
5.90
Type
journal article
Language
en
Open access
Locations
  • The FASEB Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK078966, R21 DK092718)
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
white adipocytes, bone marrow progenitor cells, hematopoietic stem cell transplantation, adipose tissue, chimerism, adipocyte origin, human adipose tissue
MeSH terms
Adipose Tissue, Animals, Bone Marrow Cells, Cell Differentiation, Cell Fusion, Hematopoietic Stem Cells, Humans, Male, Mice, Transgenic, Promoter Regions, Genetic, Stem Cells, Cell Lineage, Mice, Adipocytes, White

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