De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue
Open accessAbstract
White adipocytes in adults are typically derived from tissue resident mesenchymal progenitors. The recent identification of de novo production of adipocytes from bone marrow progenitor‐derived cells in mice challenges this paradigm and indicates an alternative lineage specification that adipocytes exist. We hypothesized that alternative lineage specification of white adipocytes is also present in human adipose tissue. Bone marrow from transgenic mice in which luciferase expression is governed by the adipocyte‐restricted adiponectin gene promoter was adoptively transferred to wild‐type recipient mice. Light emission was quantitated in recipients by in vivo imaging and direct enzyme assay. Adipocytes were also obtained from human recipients of hematopoietic stem cell transplantation. DNA was isolated, and microsatellite polymorphisms were exploited to quantify donor/recipient chimerism. Luciferase emission was detected from major fat depots of transplanted mice. No light emission was observed from intestines, liver, or lungs. Up to 35% of adipocytes in humans were generated from donor marrow cells in the absence of cell fusion. Nontransplanted mice and stromal‐vascular fraction samples were used as negative and positive controls for the mouse and human experiments, respectively. This study provides evidence for a nontissue resident origin of an adipocyte subpopulation in both mice and humans.—Gavin, K. M., Gutman, J. A., Kohrt, W. M., Wei, Q., Shea, K. L., Miller, H. L., Sullivan, T. M., Erickson, P. F., Helm, K. M., Acosta, A. S., Childs, C. R., Musselwhite, E., Varella‐Garcia, M., Kelly, K., Majka, S. M., Klemm, D. J., De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue. FASEB J. 30, 1096–1108 (2016). www.fasebj.org
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Circulating stem cells can contribute to adipogenesis.
“For example, circulating stem cells can contribute to adipogenesis (19).”
From The ominous triad of adipose tissue dysfunction: inflammation, fibrosis, and impaired angiogenesis. (2017) · cites this paper as [19]
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Modulation of Energy Expenditure by Estrogens and Exercise in Women
Gavin, K. M., Kohrt, W. M., Klemm, D. J., & Melanson, E. L. (2018) · Exercise and Sport Sciences Reviews · doi:10.1249/jes.0000000000000160 · Cited by 43 · see citing works
Self-citationIdentifying the Critical Gaps in Research on Sex Differences in Metabolism Across the Life Span
Reusch, J. E. B., Kumar, T. R., Regensteiner, J. G., Zeitler, P. S., Participants, C., Arany, Z. P., Merz, C. N. B., Barrett-Connor, E. L., Boyle, K. E., Brown, L., et al. (2017) · Endocrinology · doi:10.1210/en.2017-03019 · Cited by 38 · see citing works
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Flow cytometric single cell analysis reveals heterogeneity between adipose depots
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Ovarian Hormones Regulate the Production of Adipocytes From Bone Marrow-Derived Cells
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Self-citationAdipose Organ Development and Remodeling
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Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D
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Single‐Cell Sorting of Non‐human Primate Adipocytes with Large‐particle Flow Cytometry
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Self-citation
- Authors
- Gavin, K. M., Gutman, J. A., Kohrt, W. M., Wei, Q., Shea, K. L., Miller, H. L., Sullivan, T. M., Erickson, P. F., Helm, K. M., Acosta, A. S., Childs, C. R., Musselwhite, E., Varella‐Garcia, M., Kelly, K., Majka, S. M., & Klemm, D. J.
- Affiliations
- University of Colorado Anschutz (US)
- Children's Hospital Colorado (US)
- University of Colorado Cancer Center (US)
- Vanderbilt University (US)
- Corresponding author
- Klemm, Dwight J.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2015-11-18
- Volume
- 30
- Issue
- 3
- Pages
- 1096-1108
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- PMC
- PMC4750419
- OpenAlex
- W2177636187
- Cited by
- 55
- References
- 69
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 9 · 2020: 3 · 2019: 4 · 2018: 9 · 2017: 9 · 2016: 6
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The FASEB Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK078966, R21 DK092718)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- white adipocytes, bone marrow progenitor cells, hematopoietic stem cell transplantation, adipose tissue, chimerism, adipocyte origin, human adipose tissue
- MeSH terms
- Adipose Tissue, Animals, Bone Marrow Cells, Cell Differentiation, Cell Fusion, Hematopoietic Stem Cells, Humans, Male, Mice, Transgenic, Promoter Regions, Genetic, Stem Cells, Cell Lineage, Mice, Adipocytes, White
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