Cited by 454Adipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and MetabolismPeroxisome Proliferator-Activated Receptors

Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression

Jernås, Margareta, Palming, Jenny, Sjöholm, Kajsa, Jennische, Eva, Svensson, Per-Arne, Gabrielsson, Britt G., Levin, Max, Sjögren, Anders +18 more·2006·The FASEB Journal

Abstract

Enlarged adipocytes are associated with insulin resistance and are an independent predictor of type 2 diabetes. To understand the molecular link between these diseases and adipocyte hypertrophy, we developed a technique to separate human adipocytes from an adipose tissue sample into populations of small cells (mean 57.6±3.54 pm) and large cells (mean 100.1±3.94 pm). Microarray analysis of the cell populations separated from adipose tissue from three subjects identified 14 genes, of which five immune‐related, with more than fourfold higher expression in large cells than small cells. Two of these genes were serum amyloid A (SAA) and transmembrane 4 L six family member 1 (TM4SF1). Real‐time RT‐PCR analysis of SAA and TM4SF1 expression in adipocytes from seven subjects revealed 19‐fold and 22‐fold higher expression in the large cells, respectively, and a correlation between adipocyte size and both SAA and TM4SF1 expression. The results were verified using immunohistochemistry. In comparison with 17 other human tissues and cell types by microarray, large adipocytes displayed by far the highest SAA and TM4SF1 expression. Thus, we have identified genes with markedly higher expression in large, compared with small, human adipocytes. These genes may link hypertrophic obesity to insulin resistance/type 2 diabetes.—Jernås, M., Palming, J., Sjöholm, K., Jennische, E., Svensson, P.‐A., Gabrielsson, B. G., Levin, M., Sjögren, A., Rudemo, M., Lystig, T. C., Carlsson, B., Carlsson, L. M. S., Lönn, M. Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression. FASEB J. 20, E832–E839 (2006)

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Crossref · 10.1096/fj.05-5678fje

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  • Weight loss is accompanied by decreased circulating CRP, TNF-a, IL-6, and sICAM-1.

    “Weight loss is accompanied by decreased concentrations of circulating mediators of inflammation, such as CRP, TNF-a, IL-6 and sICAM-1 (155,268 -270) , although it is difficult to dissect whether this effect is due to the weight loss per se or to the nature of the diet used to induce weight loss.”

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Authors
Jernås, M., Palming, J., Sjöholm, K., Jennische, E., Svensson, P.-A., Gabrielsson, B. G., Levin, M., Sjögren, A., Rudemo, M., Lystig, T. C., Carlsson, B., Carlsson, L. M. S., Lonn, M., Jernås, M., Palming, J., Sjöholm, K., Jennische, E., Svensson, P.-A., Gabrielsson, B. G., Levin, M., Sjögren, A., Rudemo, M., Lystig, T. C., Carlsson, B., Carlsson, L. M. S., & Lonn, M.
Venue
The FASEB Journal
Publisher
Wiley
Published
2006-06-05
Volume
20
Issue
9
Pages
1540-1542
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2166193349
Cited by
454
References
55
Citations by year
2026: 7 · 2025: 13 · 2024: 8 · 2023: 17 · 2022: 17 · 2021: 31 · 2020: 18 · 2019: 27 · 2018: 23 · 2017: 18 · 2016: 25 · 2015: 26 · 2014: 28 · 2013: 36 · 2012: 28
FWCI
18.55
Type
journal article
Language
en
Open access
closed
Locations
  • The FASEB Journal (publishedVersion)
  • PubMed (publishedVersion)
  • Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
  • Chalmers Research (Chalmers University of Technology) (submittedVersion)
Funders
  • Vetenskapsrådet (521-2002-6480, 529-2002-6671, 521-2002-6356, 521-2005-6736, 11285)
  • Diabetesförbundet
  • Socialstyrelsen
  • Stiftelserna Wilhelm och Martina Lundgrens
  • Adlerbertska Stiftelserna
  • Åke Wiberg Stiftelse
  • Magnus Bergvalls Stiftelse
  • Fredrik och Ingrid Thurings Stiftelse
  • Tore Nilsons Stiftelse för Medicinsk Forskning
Topics
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
adipocyte hypertrophy, adipocyte size, human adipocytes, serum amyloid A, TM4SF1, gene expression, insulin resistance, type 2 diabetes, obesity, immune-related genes, adipose tissue, size-based cell separation
MeSH terms
Female, Gene Expression Regulation, Humans, Hypertrophy, Insulin Resistance, Male, Adipocytes, Postmenopause, Reverse Transcriptase Polymerase Chain Reaction, Leptin, Cell Size

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