Single-nucleus transcriptomics reveal disrupted pathways in the prefrontal cortex of Scn2a -deficient mice
Open accessAbstract
Truncating variants in SCN2A , which encodes the NaV1.2 sodium channel critical for action potential initiation and propagation, are associated with autism spectrum disorder (ASD) and epilepsy. To investigate SCN2A deficiency–related phenotypes, we developed a preclinical mouse model with under 50% NaV1.2 expression, exhibiting neuronal hyperexcitability and social deficits. However, the neuronal populations and molecular alterations underlying these phenotypes at single-cell resolution have not been investigated. In this study, we conducted single-nucleus RNA sequencing (snRNA-seq) of wild-type (WT), homozygous Scn2a -deficient (HOM) mice, and HOM mice with Scn2a restoration (HOM-FlpO) to examine the effects of Scn2a level on gene expression in the medial prefrontal cortex (mPFC), a critical brain region related to ASD development. Differential expression analysis in GABAergic and glutamatergic neurons between genotypes revealed gene expression enriched in neurotransmitter activity regulation and synapse organization. Lastly, snRNA-seq results in HOM-FlpO identified genes that were rescued after Scn2a restoration. These results reveal that reduced Scn2a expression disrupts RNA transcriptomes in multiple neuronal subtypes, providing insight into cell type–specific mechanisms underlying SCN2A -related disorders.
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- Authors
- Yoo, Y.-E., Mandal, P., Tang, Z., Zhang, Z., Zhang, J., Chen, X., Robinson, M., Eaton, M., Deming, B., Halurkar, M., Kothandaraman, H., Dabin, L. C., Jiang, B., Gao, H., Yuan, C., Lanman, N., Liu, Y., Kim, J., Baloni, P., & Yang, Y.
- Affiliations
- Purdue University West Lafayette (US)
- Indiana University Indianapolis (US)
- Indiana University School of Medicine
- Davidson College (US)
- Corresponding author
- Yang, Yang
- ORCID
- Venue
- bioRxiv (Cold Spring Harbor Laboratory)
- Publisher
- openRxiv
- Published
- 2025-09-17
- PMC
- PMC12458409
- OpenAlex
- W4414369925
- Cited by
- 1
- References
- 74
- Citations by year
- 2025: 1
- Type
- posted content
- Language
- en
- Open access
- green
- doi.org
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- Locations
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (submittedVersion, cc-by-nc-nd)
- PubMed Central (submittedVersion, cc-by-nc-nd)
- Funders
- National Institute of Neurological Disorders and Stroke (R01NS117585, R01NS123154)
- Indiana University Melvin and Bren Simon Comprehensive Cancer Center (Grant P30CA082709)
- Purdue University Institute for Cancer Research (Grant P30CA023168)
- Walther Cancer Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
- Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
- Keywords
- SCN2A, Nav1.2 sodium channel, autism spectrum disorder, epilepsy, medial prefrontal cortex, neuronal hyperexcitability, social dysfunction, single-nucleus transcriptomics, GABAergic neurons, glutamatergic neurons, synaptic organization, neuronal transcriptome, gene rescue, mouse model
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