NAD + supplementation rejuvenates aged gut adult stem cells
Open accessAbstract
The tissue decline due to aging is associated with the deterioration of adult stem cell function. Here we show the number and proliferative activity of intestinal stem cells (ISCs) but not Paneth cells decline during aging, as does ISC function assessed ex vivo. Levels of SIRT1 and activity of mTORC1 also decline with aging. The treatment with the NAD(+) precursor nicotinamide riboside (NR) rejuvenates ISCs from aged mice and reverses an impaired ability to repair gut damage. The effect of NR is blocked by the mTORC1 inhibitor rapamycin or the SIRT1 inhibitor EX527. These findings demonstrate that small molecules affecting the NAD/SIRT1/mTORC1 axis may guide a translational path for maintenance of the intestine during aging.
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NAD precursors such as nicotinamide riboside can restore ageing defects in ISCs, as well as in MuSCs, NSCs and melanocyte stem cells.
“NAD precursors such as nicotinamide riboside can restore ageing defects in ISCs120, as well as in MuSCs, NSCs and melanocyte stem cells39.”
From Ageing and rejuvenation of tissue stem cells and their niches (2023) · cites this paper as [120]
Nicotinamide riboside administration reverses the age-related impairment of ISC ability to repair injury in the gut.
“Nicotinamide riboside administration on ISCs revealed a reversal of the age-related impairment of the ability of these cells to repair injury in the gut120.”
From Ageing and rejuvenation of tissue stem cells and their niches (2023) · cites this paper as [120]
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- Authors
- Igarashi, M., Miura, M., Williams, E., Jaksch, F., Kadowaki, T., Yamauchi, T., & Guarente, L.
- Affiliations
- The University of Tokyo (JP)
- Massachusetts Institute of Technology (US)
- ChromaDex (United States) (US)
- Corresponding author
- Yamauchi, Toshimasa
- Guarente, Leonard
- Venue
- Aging Cell
- Publisher
- Wiley
- Published
- 2019-03-27
- Volume
- 18
- Issue
- 3
- Pages
- e12935-e12935
- ISSN
- 1474-9718 (ISSN-L)
- 1474-9726
- PMC
- PMC6516145
- OpenAlex
- W2932874347
- Cited by
- 138
- References
- 32
- Citations by year
- 2026: 10 · 2025: 17 · 2024: 15 · 2023: 16 · 2022: 20 · 2021: 27 · 2020: 24 · 2019: 9
- FWCI
- 10.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Aging Cell (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Funders
- Japan Society for the Promotion of Science (JP17H06631)
- Ube Industries
- Glenn Foundation for Medical Research
- Takeda Science Foundation
- Manpei Suzuki Diabetes Foundation
- Foundation for the National Institutes of Health
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Keywords
- NAD+ supplementation, nicotinamide riboside, intestinal stem cells, aging, SIRT1, mTORC1, Paneth cells, stem cell rejuvenation, rapamycin, EX527
- MeSH terms
- Aging, Animals, Carbazoles, Cells, Cultured, Intestinal Mucosa, Male, Mice, Inbred C57BL, NAD, Niacinamide, Pyridinium Compounds, Rejuvenation, Dextran Sulfate, Sirolimus, Cell Proliferation, Mice, Adult Stem Cells
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