Subchronic treatment of rats with oxytocin results in improved adipocyte differentiation and increased gene expression of factors involved in adipogenesis
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BACKGROUND AND PURPOSE: Treatment with thiazolidinediones, insulin-sensitizing drugs, enhances adipogenesis, which may result in unwanted increase in adiposity. Based on the suggested metabolic effects of oxytocin, the aims of the present study were to: (i) determine whether chronic treatment with oxytocin exerts positive effects on white adipose tissue growth without increasing adiposity; (ii) investigate possible mechanisms of action of oxytocin by measuring the level of gene expression of adipogenic factors; and (iii) test the hypothesis that oxytocin's effect on adipose tissue involves specific activation of eukaryotic elongation factor 2 (eEF2). EXPERIMENTAL APPROACH: Adult rats were subcutaneously treated with oxytocin (3.6 µg·100 g⁻¹ body weight day⁻¹) via osmotic minipumps for 2 weeks. Adipocytes from epididymal adipose tissue were isolated and their size evaluated by light microscopy. Gene expression of adipogenic and angiogenic factors was determined by real-time PCR and dephosphorylation of eEF2 by immunoblotting. KEY RESULTS: Oxytocin treatment decreased the diameter of adipocytes and increased the epididymal adipose tissue protein content without changing the adipose tissue mass. Increases in fatty acid binding protein, peroxisome proliferator-activated receptor γ, insulin-sensitive glucose transporter 4, leptin and CD31 mRNA levels were noted in the epididymal and/or retroperitoneal fat tissue of oxytocin-treated rats. Oxytocin enhanced the dephosphorylation of eEF2 in the epididymal adipose tissue. CONCLUSIONS AND IMPLICATIONS: The present results demonstrate that subchronic treatment with oxytocin induces adipogenic and angiogenic effects and that the eEF2 signalling pathway is involved in these effects of oxytocin on adipose tissue in vivo. These findings are likely to motivate further research and indicate new approaches for modulating adipose tissue morphology and metabolism.
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Peripheral oxytocin administration in rats increases insulin-sensitive glucose transporter 4 mRNA in fat, suggesting a mechanism for increasing insulin sensitivity.
“Peripheral oxytocin administration in rats increases insulin-sensitive glucose transporter 4 mRNA in fat, suggesting a mechanism for increasing insulin sensitivity(39).”
From Oxytocin reduces caloric intake in men (2015) · cites this paper as [39]
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- Authors
- Eckertova, M., Ondrejcakova, M., Krskova, K., Zorad, S., & Jezova, D.
- Venue
- British Journal of Pharmacology
- Publisher
- Wiley
- Published
- 2010-12-14
- Volume
- 162
- Issue
- 2
- Pages
- 452-463
- ISSN
- 0007-1188 (ISSN-L)
- 1476-5381
- PMC
- PMC3031065
- OpenAlex
- W1522228430
- Cited by
- 68
- References
- 51
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 4 · 2020: 5 · 2019: 8 · 2018: 6 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 4 · 2013: 5 · 2012: 2
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal of Pharmacology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
- Topics
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- oxytocin, adipogenesis, white adipose tissue, elongation factor 2, PPARγ, fatty acid binding protein, glucose transporter 4, leptin, CD31, angiogenesis, epididymal adipose tissue, retroperitoneal fat, adiposity, thiazolidinediones
- MeSH terms
- Animals, Drug Evaluation, Preclinical, Male, Oxytocin, RNA, Messenger, Time Factors, Gene Expression, Rats, Wistar, Adipocytes, Peptide Elongation Factor 2, PPAR gamma, Adipogenesis, Fatty Acid-Binding Proteins, Rats, Adipose Tissue, White
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