Whole genome sequence analysis and in vitro probiotic characteristics of a Lactobacillus strain Lactobacillus paracasei ZFM54
Abstract
AIM: The aim was to identify a Lactobacillus strain with potential probiotic characteristics by whole-genome sequence analysis and in vitro experimental studies. METHODS AND RESULTS: The whole-genome sequencing was carried out using PacBio RSII sequencing method and Illumina's paired-end sequencing technology. Gene prediction and annotation were achieved using GlimmerVersion 3.02 and NCBI prokaryotic Genome Annotation Pipeline. Identification was done by biochemical tests and 16S rRNA sequence analysis. mega 6 software was used to build phylogenetic tree. Antagonism against pathogen was determined by agar well diffusion method. Resistance and stability to bile, simulated gastric acid, different salt concentration and thermostability were investigated. Hydrophobicity assay, aggregation assay and anti-oxidation assay were performed to check further probiotic traits. Finally antibiotic susceptibility and acute oral toxicity of the strain in mice were investigated to check its safety status. The strain showed >99% similarity to Lactobacillus paracasei which was further confirmed by biochemical tests. It significantly inhibited pathogens in agar well diffusion assay. It showed tolerance to simulated gastric acid (pH 3), 0·3% bile salt and 10% NaCl. Significant hydrophobic, aggregation and anti-oxidizing activities were observed. No resistance to antibiotics tested was observed and no adverse effects during acute oral toxicity in mice were detected. CONCLUSIONS: Lactobacillus paracasei ZFM 54, a new and safe Lactobacillus strain was identified with numerous probiotic-associated genes and characteristics confirmed by experimental studies. SIGNIFICANCE AND IMPACT OF THE STUDY: A new probiotic strain has been identified which is highly stable, safe and suitable to be used in health and food industries.
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WGS analysis provides a better understanding of the relation between strains' genotypic and phenotypic profiles and is required to better understand strain features.
“The WGS analysis also provides a better understanding of the relation between strains’ genotypic and phenotypic profiles and, thus, is required to better understand strain features [17].”
From Genomic Characterization of Lactiplantibacillus plantarum Strains: Potential Probiotics from Ethiopian Traditional Fermented Cottage Cheese (2024) · cites this paper as [17]
Cites
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013) · Molecular biology and evolution · doi:10.1093/molbev/mst197 · Cited by 41,174 · see citing works
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
Lowe, T. M., & Eddy, S. R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.5.955 · Cited by 11,120 · see citing works
Hill (2014) · doi:10.1038/nrgastro.2014.66 · Cited by 9,592 · see citing works
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Lagesen, K., Hallin, P., Rødland, E., Stærfeldt, H., Rognes, T., & Ussery, D. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm160 · Cited by 5,965 · see citing works
Identifying bacterial genes and endosymbiont DNA with Glimmer
Delcher, A. L., Bratke, K. A., Powers, E. C., & Salzberg, S. L. (2007) · Bioinformatics · doi:10.1093/bioinformatics/btm009 · Cited by 3,141 · see citing works
Bacteriocins: Developing innate immunity for food
Cotter, P. D., Hill, C., & Ross, R. P. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1273 · Cited by 2,380 · see citing works
PacBio Sequencing and its Applications
Rhoads, A., & Au, K. F. (2015) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2015.08.002 · Cited by 2,338 · see citing works
Probiotics: an overview of beneficial effects
Ouwehand, A. C., Salminen, S., & Isolauri, E. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020620607611 · Cited by 1,045 · see citing works
Dunne, C., O’Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O’Halloran, S., Feeney, M., Flynn, S., Fitzgerald, G., Daly, C., et al. (2001) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.386s · Cited by 922 · see citing works
Antioxidant Properties of Probiotic Bacteria
Wang, Y., Wu, Y., Wang, Y., Xu, H., 梅小强, Yu, D., Wang, Y., & Li, W. (2017) · Nutrients · doi:10.3390/nu9050521 · Cited by 922 · see citing works
(FEEDAP), E. P. O. A. A. P. O. S. U. I. A. F. (2012) · EFSA Journal · doi:10.2903/j.efsa.2012.2740 · Cited by 904 · see citing works
Benefaction of probiotics for human health: A review
George Kerry, R., Patra, J. K., Gouda, S., Park, Y., Shin, H.-S., & Das, G. (2018) · Journal of Food and Drug Analysis · doi:10.1016/j.jfda.2018.01.002 · Cited by 898 · see citing works
Kos, B., Šušković, J., Vuković, S., Šimpraga, M., Frece, J., & Matošić, S. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01915.x · Cited by 880 · see citing works
Probiotic species in the modulation of gut microbiota: An overview
Azad, M. A. K., Sarker, M., Li, T., & Yin, J. (2018) · BioMed Research International · doi:10.1155/2018/9478630 · Cited by 803 · see citing works
Charteris, Kelly, Morelli, & Collins (1998) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1998.00407.x · Cited by 774 · see citing works
Teuber, M. (2001) · Wiley eBooks · doi:10.1002/9783527620999.ch10 · Cited by 737 · see citing works
Probiotic Bacterium Prevents Cytokine-induced Apoptosis in Intestinal Epithelial Cells*
Yan, F., & Polk, D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m207050200 · Cited by 599 · see citing works
Lactic acid bacteria contribution to gut microbiota complexity: lights and shadows.
Pessione, E. (2012) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2012.00086 · Cited by 592 · see citing works
Probiotics: determinants of survival and growth in the gut
Bezkorovainy, A. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.399s · Cited by 533 · see citing works
Immunomodulatory Effects of Probiotics on Cytokine Profiles
Azad, M. A. K., Sarker, M., & Wan, D. (2018) · BioMed Research International · doi:10.1155/2018/8063647 · Cited by 521 · see citing works
Son, S. M., & Lewis, B. A. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf010830b · Cited by 511 · see citing works
Antioxidative Ability of Lactic Acid Bacteria
Lin, M., & Yen, C.-L. (1999) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf981149l · Cited by 511 · see citing works
Cholesterol Assimilation by Lactic Acid Bacteria and Bifidobacteria Isolated from the Human Gut
Pereira, D. I. A., & Gibson, G. R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.9.4689-4693.2002 · Cited by 481 · see citing works
Bacteriocins Produced by Lactic Acid Bacteria a Review Article
Zacharof, M., & Lovitt, R. W. (2012) · APCBEE Procedia · doi:10.1016/j.apcbee.2012.06.010 · Cited by 431 · see citing works
Gardiner, G. E., O'Sullivan, E., Kelly, J., Auty, M. A. E., Fitzgerald, G. F., Collins, J. K., Ross, R. P., & Stanton, C. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2605-2612.2000 · Cited by 414 · see citing works
Discovering probiotic microorganisms: in vitro, in vivo, genetic and omics approaches
Papadimitriou, K., Ζουμποπούλου, Γ., Foligné, B., Alexandraki, V., Κάζου, Μ., Pot, B., & Tsakalidou, E. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00058 · Cited by 388 · see citing works
Angmo, K., Kumari, A., Savitri, & Bhalla, T. C. (2015) · LWT · doi:10.1016/j.lwt.2015.10.057 · Cited by 381 · see citing works
Nagpal, R., Wang, S., Ahmadi, S., Hayes, J., Gagliano, J., Subashchandrabose, S., Kitzman, D. W., Becton, T., Read, R., & Yadav, H. (2018) · Scientific Reports · doi:10.1038/s41598-018-30114-4 · Cited by 355 · see citing works
Vizoso-Pinto, M. G., Franz, C. M. A. P., Schillinger, U., & Holzapfel, W. H. (2006) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2006.01.029 · Cited by 328 · see citing works
Polysaccharide Production by Lactic Acid Bacteria: From Genes to Industrial Applications
Zeidan, A. A., Poulsen, V. K., Janzen, T., Buldo, P., Derkx, P. M. F., Øregaard, G., & Neves, A. R. (2017) · FEMS Microbiology Reviews · doi:10.1093/femsre/fux017 · Cited by 326 · see citing works
Bergonzelli, G. E., Granato, D., Pridmore, R. D., Marvin, L., Donnicola, D., & Corthesy-Theulaz, I. E. (2005) · Infection and Immunity · doi:10.1128/iai.74.1.425-434.2006 · Cited by 302 · see citing works
Functional and Probiotic Attributes of an Indigenous Isolate of Lactobacillus plantarum
Kaushik, J. K., Kumar, A., Duary, R. K., Mohanty, A. K., Grover, S., & Batish, V. K. (2009) · PLoS ONE · doi:10.1371/journal.pone.0008099 · Cited by 295 · see citing works
BAGEL: a web-based bacteriocin genome mining tool
de Jong, A., van Hijum, S. A. F. T., Bijlsma, J. J. E., Kok, J., & Kuipers, O. P. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl237 · Cited by 289 · see citing works
Strategies to improve the functionality of probiotics in supplements and foods
Champagne, C. P., da Cruz, A. G., & Daga, M. (2018) · Current Opinion in Food Science · doi:10.1016/j.cofs.2018.04.008 · Cited by 263 · see citing works
Prasad, J., McJarrow, P., & Gopal, P. K. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.2.917-925.2003 · Cited by 231 · see citing works
Boza, a natural source of probiotic lactic acid bacteria
Todorov, S. D., Botes, M., Guigas, C., Schillinger, U., Wiid, I. J. F., Wachsman, M. B., Holzapfel, W. H., & Dicks, L. M. T. (2007) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2007.03558.x · Cited by 209 · see citing works
In vitro testing of commercial and potential probiotic lactic acid bacteria
Jensen, H., Grimmer, S., Naterstad, K., & Axelsson, L. T. (2011) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2011.11.020 · Cited by 205 · see citing works
Axelsson, L. T. (2004) · CRC Press eBooks · doi:10.1201/9780824752033.ch1 · Cited by 178 · see citing works
An overview of the mosaic bacteriocin pln loci from Lactobacillus plantarum
Diep, D. B., Straume, D., Kjos, M., Torres, C., & Nes, I. F. (2009) · Peptides · doi:10.1016/j.peptides.2009.05.014 · Cited by 177 · see citing works
Oh, Y. J., & Jung, D. S. (2015) · LWT · doi:10.1016/j.lwt.2015.03.005 · Cited by 172 · see citing works
Ren, D., Li, C., Qin, Y., Yin, R., Du, S., Ye, F., Liu, C., Liu, H., Wang, M., Li, Y., et al. (2014) · Anaerobe · doi:10.1016/j.anaerobe.2014.07.004 · Cited by 163 · see citing works
Adnan, A. F. M., & Tan, I. K. P. (2006) · Bioresource Technology · doi:10.1016/j.biortech.2006.05.034 · Cited by 150 · see citing works
Wang, Y., Wu, Y., Wang, Y., Fu, A., Gong, L., Li, W., & Li, Y. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-8032-4 · Cited by 145 · see citing works
Curto, A. L., Pitino, I., Mandalari, G., Dainty, J. R., Faulks, R. M., & Wickham, M. S. J. (2011) · Food Microbiology · doi:10.1016/j.fm.2011.06.007 · Cited by 144 · see citing works
MASUDA, M., Mariko, I. D. E., Utsumi, H., NIIRO, T., Shimamura, Y., & Murata, M. (2012) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.120414 · Cited by 131 · see citing works
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak
Khalil, E. S., Manap, M. Y. A., Mustafa, S., Alhelli, A. M., & Shokryazdan, P. (2018) · Molecules · doi:10.3390/molecules23020398 · Cited by 128 · see citing works
Novel isolates of lactobacilli from fermented Portuguese olive as potential probiotics
Peres, C. M., Alves, M., Hernández‐Mendoza, A., Moreira, L., da Silva, S. R. A., Bronze, M. R., Vilas-Boas, L., Peres, C., & Malcata, F. X. (2014) · LWT · doi:10.1016/j.lwt.2014.03.003 · Cited by 119 · see citing works
Pan, W.-H., Li, P., & Liu, Z. (2006) · Anaerobe · doi:10.1016/j.anaerobe.2006.03.001 · Cited by 107 · see citing works
Self-citationIzquierdo, E., Horvatovich, P. L., Marchioni, E., Werner, D., Sanz, Y., & Ennahar, S. (2009) · Electrophoresis · doi:10.1002/elps.200800399 · Cited by 106 · see citing works
Ku, S., Park, M., Ji, G., & You, H. J. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17091544 · Cited by 106 · see citing works
Buriti, F. C. A., Cardarelli, H. R., Filisetti, T. M. C. C., & Saad, S. M. I. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.03.001 · Cited by 103 · see citing works
Functional Analysis of the p40 and p75 Proteins from Lactobacillus casei BL23
Bäuerl, C., Pérez‐Martínez, G., Yan, F., Polk, D. B., & Monedero, V. (2010) · Microbial Physiology · doi:10.1159/000322233 · Cited by 103 · see citing works
Characterization of the intestinal microbiota and its interaction with probiotics and health impacts
de Almada, C. N., de Almada, C. N., Martinez, R. C. R., & Sant’Ana, A. S. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-6582-5 · Cited by 101 · see citing works
Hu, C.-B., Malaphan, W., Zendo, T., Nakayama, J., & Sonomoto, K. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.02264-09 · Cited by 92 · see citing works
Antimicrobial and antidiabetic potential of synbiotic fermented milk: A functional dairy product
Shafi, A., Raja, H. N., Farooq, U., Akram, K., Hayat, Z., Naz, A., & Nadeem, H. R. (2018) · International Journal of Dairy Technology · doi:10.1111/1471-0307.12555 · Cited by 80 · see citing works
Recent Advances in the Role of Probiotics in Human Inflammation and Gut Health
Rupa, P., & Mine, Y. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf301903t · Cited by 77 · see citing works
[44] Measurement of microbial adhesion to hydrophobic substrata
Doyle, R. J., & Rosenberg, M. (1995) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(95)53046-0 · Cited by 68 · see citing works
Xu, Y., Tian, Y., Cao, Y., Li, J., Guo, H., Su, Y., Tian, Y., Wang, C., Wang, T., & Zhang, L. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00937 · Cited by 62 · see citing works
Peng, X., Zhang, R., Duan, G., Wang, C., Sun, N., Zhang, L., Chen, S., Fan, Q., & Xi, Y. (2018) · Scientific Reports · doi:10.1038/s41598-018-24879-x · Cited by 56 · see citing works
Chondrou, P., Karapetsas, A., Kiousi, D. E., Tsela, D., Tiptiri-Kourpeti, A., Anestopoulos, I., Kotsianidis, Ι., Bezirtzoglou, E., Pappa, A., & Galanis, Α. (2018) · Beneficial Microbes · doi:10.3920/bm2017.0183 · Cited by 55 · see citing works
Probiotic Prato cheese attenuates cigarette smoke-induced injuries in mice
Vasconcelos, F. M., Silva, H. L. A., Poso, S. M. V., Barroso, M. V., Lanzetti, M., Rocha, R. S., da Graça, J. S., Esmerino, E. A., Freitas, M. Q., Silva, M. C., et al. (2019) · Food Research International · doi:10.1016/j.foodres.2019.06.001 · Cited by 53 · see citing works
Characterization of Indigenous Lactobacillus Strains for Probiotic Properties
Mojgani, N., Hussaini, F., & Vaseji, N. (2015) · Jundishapur Journal of Microbiology · doi:10.5812/jjm.17523 · Cited by 50 · see citing works
Cordeiro, M. A., Souza, E. L. S., Arantes, R. M. E., Balthazar, C. F., Guimarães, J. T., Scudino, H., Silva, H. L. A., Rocha, R. S., Freitas, M. Q., Esmerino, E. A., et al. (2019) · Journal of Dairy Science · doi:10.3168/jds.2019-16340 · Cited by 47 · see citing works
Hamon, E., Horvatovich, P. L., Marchioni, E., Werner, D., & Ennahar, S. (2013) · Journal of Applied Microbiology · doi:10.1111/jam.12339 · Cited by 46 · see citing works
Stefanovic, E., & McAuliffe, O. E. (2018) · BMC Genomics · doi:10.1186/s12864-018-4586-0 · Cited by 46 · see citing works
Surachat, K., Sangket, U., Deachamag, P., & Chotigeat, W. (2017) · PLoS ONE · doi:10.1371/journal.pone.0183548 · Cited by 43 · see citing works
Salzillo, M., Vastano, V., Capri, U., Muscariello, L., Sacco, M., & Marasco, R. (2015) · Journal of Basic Microbiology · doi:10.1002/jobm.201400942 · Cited by 37 · see citing works
Gu, Q., Song, D., & Muyuan, Z. (2009) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.2009.00566.x · Cited by 37 · see citing works
Self-citation[Bacteriocins produced by lactic acid bacteria].
Bilková, A., Sepová, H. K., Bilka, F., & Balážová, A. (2011) · PubMed · Cited by 33 · see citing works
Jones, R. M. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-804024-9.00009-4 · Cited by 30 · see citing works
Deng, K., Chen, T., Wu, Q., Xin, H., Wei, Q., Hu, P., Wang, X., Wang, X., Wei, H., & Shah, N. P. (2015) · Journal of Dairy Science · doi:10.3168/jds.2015-9761 · Cited by 29 · see citing works
Liu, W., Chen, Y. F., Kwok, L. Y., Li, M., Sun, T., Sun, C., Wang, X., Dan, T., Menghebilige, Zhang, H. P., et al. (2013) · Journal of Dairy Science · doi:10.3168/jds.2013-6582 · Cited by 29 · see citing works
Glutathione-mediated response to acid stress in the probiotic bacterium, Lactobacillus salivarius
Lee, K., Pi, K., Kim, E. B., Rho, B., Kang, S., Lee, H., & Choi, Y. (2010) · Biotechnology Letters · doi:10.1007/s10529-010-0244-6 · Cited by 26 · see citing works
Assessment of potential probiotic bacteria isolated from milk of domestic animals.
Tambekar, D. H., Bhutada, S. A., Choudhary, S., & Khond, M. (2009) · Journal of Applied Biosciences · Cited by 19 · see citing works
Flagged first, then by citations
2 of the 76 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Castro-López, C., García, H. S., Guadalupe Martínez-Ávila, G. C., González-Córdova, A. F., Vallejo-Cordoba, B., & Hernández-Mendoza, A. (2021) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2020.12.017 · Cited by 72 · see citing works
Purification and characterization of a novel bacteriocin from Lactobacillus paracasei ZFM54
Ye, P., Wang, J., Liu, M., Li, P., & Gu, Q. (2021) · LWT · doi:10.1016/j.lwt.2021.111125 · Cited by 69 · see citing works
Self-citationEmerging pre-clinical safety assessments for potential probiotic strains: a review
Nataraj, B. H., Behare, P. V., Yadav, H., & Srivastava, A. K. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2197066 · Cited by 71 · see citing works
Somashekaraiah, R., Mottawea, W., Gunduraj, A., Joshi, U., Hammami, R., & Sreenivasa, M. Y. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.696267 · Cited by 66 · see citing works
Jiang, Y., Yang, R.-S., Lin, Y., Xin, W., Zhou, H.-Y., Wang, F., Zhang, Q., & Lin, L. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1120263 · Cited by 46 · see citing works
Kim, E., Yang, S.-M., Kim, D., & Kim, H. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.794315 · Cited by 42 · see citing works
Zhao, X., Liang, Q., Song, X., & Zhang, Y. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1146566 · Cited by 35 · see citing works
In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
Zhang, Q., Wang, M., Ma, X., Li, Z., Jiang, C., Pan, Y., & Zeng, Q. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2022.984537 · Cited by 38 · see citing works
Chen, X., Zhu, Z., Zhang, X., Chen, L., Gu, Q., & Li, P. (2024) · Journal of Dairy Science · doi:10.3168/jds.2023-24332 · Cited by 30 · see citing works
Self-citationZhou, Q., Qureshi, N., Xue, B., Xie, Z., Li, P., & Gu, Q. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.972569 · Cited by 30 · see citing works
Self-citationNshimiyimana, S., Mustopa, A. Z., Budiarti, S., Meilina, L., Hertati, A., & Handayani, I. (2023) · Journal of Genetic Engineering and Biotechnology · doi:10.1186/s43141-023-00503-y · Cited by 29 · see citing works
Yu, J., Chen, Z., Zhou, Q., Li, P., Wu, S., Zhou, T., & Gu, Q. (2024) · Frontiers in Nutrition · doi:10.3389/fnut.2024.1426358 · Cited by 27 · see citing works
Self-citationRaj, C. T. D., Suryavanshi, M. V., Kandaswamy, S., Ramasamy, K. P., & James, R. A. (2023) · Genomics · doi:10.1016/j.ygeno.2023.110637 · Cited by 24 · see citing works
Dışhan, A., & Gönülalan, Z. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10216-4 · Cited by 24 · see citing works
Joishy, T. K., Bhattacharya, A., Singh, C. T., Mukherjee, A. K., & Khan, M. R. (2024) · New Biotechnology · doi:10.1016/j.nbt.2024.07.005 · Cited by 23 · see citing works
Gizachew, S., & Engidawork, E. (2024) · Genes · doi:10.3390/genes15111389 · Cited by 15 · see citing works
“The WGS analysis also provides a better understanding of the relation between strains’ genotypic and phenotypic profiles and, thus, is required to better understand strain features [17].”
Chen, T., Zhao, Y., Fan, Y., Dong, Y., & Gai, Z. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1501502 · Cited by 16 · see citing works
Lee, M., Bang, W.-Y., Lee, H.-B., Yang, S., Lee, K., Kang, H., Hong, S. M., & Yang, J. (2024) · Microorganisms · doi:10.3390/microorganisms12102063 · Cited by 16 · see citing works
Colautti, A., Ginaldi, F., Camprini, L., Comi, G., Reale, A., & Iacumin, L. (2024) · Nutrients · doi:10.3390/nu16142212 · Cited by 15 · see citing works
Sun, C., Liang, Q., Zhao, B., Zhang, Y., & Chen, X. (2025) · Food Research International · doi:10.1016/j.foodres.2025.116183 · Cited by 14 · see citing works
Ruiz, M. J., Medina, L. M., Palacio, M. I., Vega, M. F., Etcheverría, S. G., Frizzo, L. S., & Etcheverría, A. I. (2024) · Small Ruminant Research · doi:10.1016/j.smallrumres.2024.107329 · Cited by 13 · see citing works
Basharat, S., Zhai, L., Jiang, F., Asjad, T., Khan, A. N., & Liao, X. (2025) · Microorganisms · doi:10.3390/microorganisms13020216 · Cited by 12 · see citing works
El-Hosseny, M. F., Seadawy, M. G., Abdel-Monem, M. O., & Hassan, M. G. (2025) · BMC Microbiology · doi:10.1186/s12866-025-03757-3 · Cited by 12 · see citing works
Tang, C., Tao, J., Sun, J. Y., Lv, F., Lu, Z., & Lu, Y. (2021) · Journal of Food Biochemistry · doi:10.1111/jfbc.13925 · Cited by 12 · see citing works
Lee, H. B., Bang, W. Y., Shin, G. R., Jeon, H. J., Jung, Y. H., & Yang, J. (2023) · Microorganisms · doi:10.3390/microorganisms12010085 · Cited by 11 · see citing works
In vitro evaluation of probiotic and antioxidant potential of Lacticaseibacillus paracasei ED25
Dasdemir, E., Arslan, N. P., Örtücü, S., Aykutoğlu, G., Özkan, H., Adıgüzel, A., & Taşkın, M. (2024) · Biologia · doi:10.1007/s11756-024-01720-7 · Cited by 11 · see citing works
Probiotic potential and safety assessment of bacteriocinogenic Enterococcus faecalis CAUM157
Elnar, A. G., & Kim, G. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1563444 · Cited by 11 · see citing works
Zhan, C., Wu, M., Fang, H., Liu, X., Pan, J., Fan, X., Wang, M., & Matsumoto, H. (2023) · Food Quality and Safety · doi:10.1093/fqsafe/fyad005 · Cited by 11 · see citing works
Raj, C. T. D., Kandaswamy, S., Suryavanshi, M. V., Ramasamy, K. P., Rajasabapathy, R., & James, R. A. (2023) · Gene · doi:10.1016/j.gene.2023.147356 · Cited by 10 · see citing works
Adedeji, O. E., Chae, S. A., Ban, O., Bang, W. Y., Kim, H., Jeon, H. J., Chinma, C. E., Yang, J., & Jung, Y. H. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-03097-0 · Cited by 8 · see citing works
El-Hosseny, M. F., Hassan, M. G., Abdel-Monem, M. O., & SEADAWY, A. R. (2025) · Scientific Reports · doi:10.1038/s41598-025-90262-2 · Cited by 7 · see citing works
Screening and Phenotyping of Lactic Acid Bacteria in Boza
Zhao, X., Pei, L., Wang, X., Luo, M., Hou, S., Ye, X., Liu, W., & Zhou, Y. (2025) · Microorganisms · doi:10.3390/microorganisms13081767 · Cited by 7 · see citing works
Genomics analysis of Lactobacillus paracasei SLP16
Zhao, X., Hu, R., Liu, Y., He, Y., Li, S., Yang, J.-G., Zhou, J., & Zhang, J. (2022) · Letters in Applied Microbiology · doi:10.1111/lam.13734 · Cited by 6 · see citing works
Characterization of a Potential Probiotic Strain in Koumiss
Zhao, H., Song, Q.-J., Guo, H., Liu, C., Yang, C. Y., Li, X., Wang, Y., Ma, Z.-P., Wang, F.-X., & Yong-jun, W. (2023) · Fermentation · doi:10.3390/fermentation9020087 · Cited by 5 · see citing works
Özdemir, N. (2023) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-023-04393-1 · Cited by 5 · see citing works
Sağiroğlu, A., Özdemir, N., & Çon, A. H. (2022) · Letters in Applied Microbiology · doi:10.1093/lambio/ovac012 · Cited by 5 · see citing works
Yogeswara, I. B. A., Mariyatun, Pramesi, P. C., & Rahayu, E. S. (2023) · Microbiology Resource Announcements · doi:10.1128/mra.00513-22 · Cited by 4 · see citing works
Functional properties of lactic acid bacteria isolated from raw goat milk and cottage cheese
Ikombayev, T., Ospanova, A., Омарова, А. Б., Horáčková, Š., Tuganbay, A., Касенова, Г. Т., Zhamurova, V. S., Sharipova, S., & Suleimenova, Z. (2025) · Journal of Agriculture and Food Research · doi:10.1016/j.jafr.2025.101822 · Cited by 3 · see citing works
Zamojska, D., Rosicka‐Kaczmarek, J., Macierzyńska-Piotrowska, E., & Nowak, A. (2025) · Microorganisms · doi:10.3390/microorganisms13020317 · Cited by 2 · see citing works
Herrera-Cardoso, E. D., Tapia-Cervantes, K. A., Cepeda–Negrete, J., Gutiérrez‐Vargas, S., & León‐Galván, M. F. (2026) · BMC Genomics · doi:10.1186/s12864-026-12798-5 · Cited by 2 · see citing works
Kim, S. J., Shin, J. S., Park, H. S., Song, J. S., Lee, K. W., Bang, W., & Cho, T. J. (2022) · Current Topic in Lactic Acid Bacteria and Probiotics · doi:10.35732/ctlabp.2022.8.1.1 · Cited by 2 · see citing works
Гаврилова, Н. Н., Ратникова, И. А., САДАНОВ, А. К., ОРАЗЫМБЕТ, С. Э., ШОРАБАЕВ, Е. Ж., Baimakhanova, B. B., & Protasiuk, L. E. (2023) · Research Journal of Pharmacy and Technology · doi:10.52711/0974-360x.2023.00682 · Cited by 3 · see citing works
Gu, Q. (2023) · Springer eBooks · doi:10.1007/978-981-99-2661-9_6 · Cited by 2 · see citing works
Self-citationElHadedy, D. E., Kim, C., Yousuf, A. B., Wang, Z., & Ndegwa, E. N. (2023) · Animals · doi:10.3390/ani13162669 · Cited by 2 · see citing works
Pan, W., Yang, J., Zhu, Y., Li, R., Qiao, M., Zhu, R.-Y., & Wang, S. (2023) · Journal of Applied Microbiology · doi:10.1093/jambio/lxad069 · Cited by 1 · see citing works
Lee, S.-Y., Kim, D., Yang, S.-M., Kim, E., & Kim, H. (2023) · Microbiology and Biotechnology Letters · doi:10.48022/mbl.2309.09003 · Cited by 1 · see citing works
Wang, Y., Ma, J., Lao, J., Tan, L., Zou, J., Mei, Y., Mao, Y., Li, Y., Chen, M., Ju, X., et al. (2025) · 3 Biotech · doi:10.1007/s13205-025-04476-2 · Cited by 1 · see citing works
Getachew, T., Ashenafi, M., Tigu, F., Birri, D. J., Naoli, A., & Desalegn, A. (2025) · BMC Microbiology · doi:10.1186/s12866-025-04326-4 · Cited by 1 · see citing works
Effect of xylo‐oligosaccharide on the in vitro proliferation of lactic acid bacteria from rabbits
Yang, J., Guo, H., Zhang, K., Zuo, Y., Zhu, Y., Zeng, Y., Gong, G., Zeng, L., Wang, Y., Chen, L., et al. (2025) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.70162 · Cited by 1 · see citing works
Xu, J., Sun, M., Chang, J., Xie, Q., Wang, Y., & Chen, L. (2025) · Archives of Microbiology · doi:10.1007/s00203-025-04495-w · Cited by 1 · see citing works
Huang, F., Lu, Y., Xiong, J., Zeng, X., Huang, Y., & Wang, L. (2025) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10834-6 · Cited by 1 · see citing works
Assessment of Probiotic Properties and Technological Potential of Lacticaseibacillus paracasei
Khalid, I., Sellam, A., Imane, E. Y., Ezrari, S., Elayachi, M., AL-Farga, A. M., Meziane, M., & Bouchra, E. G. (2025) · Industrial Biotechnology · doi:10.1177/15509087251399213 · Cited by 1 · see citing works
Sun, S., Feng, L., Sun, L., Zhu, X., Zhou, M., Li, X., & Mu, G. (2026) · Foods · doi:10.3390/foods15020271 · Cited by 1 · see citing works
Mai, Z., Wang, B., Hu, Q., Jia, Z., Wang, J., Liu, B. Y., & Ge, J. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-026-10979-y · Cited by 1 · see citing works
Chen, Y., Yang, J., Yv, T., Zhang, X.-R., Gong, G., Zhu, Y.-T., Yao, J., Wu, Y., & Zhang, Y.-H. (2025) · Journal of Applied Microbiology · doi:10.1093/jambio/lxaf246 · Cited by 1 · see citing works
Effect of xylo-oligosaccharide on in vitro proliferation of lactic acid bacteria from rabbitsPreprint
Guo, H., Yang, J., Gong, G.-Z., Zeng, L., Zhu, Y., Wang, Y., Chen, L., Chen, Y., & Zuo, Y. (2023) · Research Square · doi:10.21203/rs.3.rs-3215079/v1 · Cited by 1 · see citing works
Yang, J., Li, L., Kang, R., Zhu, Y., Zhu, R., Gong, G., Chen, Y., & Zhang, X. (2025) · Carbohydrate Polymer Technologies and Applications · doi:10.1016/j.carpta.2025.100882
Fonseca, B. R., Sabino, Y. N. V., Rocha, B. M. O., Azevedo, V. A. C., Brenig, B., Soares, S. D. C., Lemos, L. N., Tonucci, L. B., Luquetti, S. C. P. D., Paiva, A. D., et al. (2026) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.70441
Abbas, N., Ait-meddour, A., Ouled-Haddar, H., Sifour, M., Mühle, E., & Clermont, D. (2026) · Biology Bulletin · doi:10.1134/s1062359025609723
Xu, T., Chen, Q., Ma, J., Wu, K., Su, Z., You, D., Wen, R., Zhang, T., Wang, H., & 张安云 (2026) · Poultry Science · doi:10.1016/j.psj.2026.107024
Yin, Y., Huang, Y., Li, C., Pei, Z., Li, C., Chen, Z., Bai, H., Y, C., Li, J., Chen, H., et al. (2026) · Microorganisms · doi:10.3390/microorganisms14061290
Saeed, E., Korma, S. A., Li, F., Amer, A., & Li, L. (2026) · International Dairy Journal · doi:10.1016/j.idairyj.2026.106739
Chen, Y., Shi, H., Zhang, H., Chen, M., Cao, Z., Ma, C., & Xu, J. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-026-11191-8
Behbahani, B. A., Ghasemi, P., Vasiee, A., & Mousavi, Z. E. (2026) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-026-04882-x
Jaglan, P., Buttar, H. S., & Sobti, R. C. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-45154-6.00022-2
Genomic insights: Probiotics strain-specific benefits
Ramesh, Y., & Dhamodhar, P. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-45154-6.00024-6
- Authors
- Qureshi, N., Gu, Q., & Li, P.
- Affiliations
- Zhejiang Gongshang University (CN)
- Corresponding author
- Qureshi, N.
- ORCID
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-03-17
- Volume
- 129
- Issue
- 2
- Pages
- 422-433
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W3009622181
- Cited by
- 64
- References
- 76
- Citations by year
- 2026: 10 · 2025: 17 · 2024: 9 · 2023: 17 · 2022: 7 · 2021: 3 · 2020: 1
- FWCI
- 4.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (318775, 31601449, LY16C200002)
- Natural Science Foundation of Zhejiang Province
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Lactobacillus paracasei, probiotic strains, whole genome sequencing, bacterial identification, pathogen antagonism, bile tolerance, salt tolerance, hydrophobicity, cell aggregation, antioxidant activity, antibiotic susceptibility, acute oral toxicity, probiotic safety
- MeSH terms
- Lacticaseibacillus paracasei, Animals, Antibiosis, Anti-Bacterial Agents, Bile Acids and Salts, Phylogeny, RNA, Ribosomal, 16S, Genome, Bacterial, Sequence Analysis, DNA, Probiotics, Mice, Food Safety
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