Biofilm formation by Candida auris isolated from colonising sites and candidemia cases
Abstract
BACKGROUND: Candida auris, an emerging nosocomial pathogen, exhibits phenotypic variation. Non-aggregating C. auris isolates display greater biofilm-forming capacity and virulence than aggregate-forming isolates. Most of the studies till date have focused on clinical isolates. The biofilm-forming capacity of colonising isolates remains uninvestigated. OBJECTIVES: The present study aimed to elucidate the biofilm-forming capacity of the colonising isolates of C. auris, correlate it with their aggregation behaviour and antifungal susceptibility, and compare it with that of the isolates from blood-stream infection. METHODS: Colonising and clinical (candidemia) isolates of C. auris were screened for aggregation behaviour, biofilm-forming capacity and antifungal susceptibility testing. Aggregation behaviour was assessed microscopically. Biofilm-forming capacity was determined on 96-well flat-bottomed microtitre plates. Antifungal susceptibility testing was performed by broth microdilution assay. RESULTS: Aggregative and non-aggregative phenotypes were found to be predominantly associated with colonising and clinical isolates, respectively, with the former ones being stronger biofilm producers in the colonising group. Non-aggregative isolates in the colonising group showed lower susceptibility to amphotericin B and fluconazole than aggregative isolates. In contrast, no association was noted between biofilm formation, aggregation behaviour and antifungal susceptibility amongst the clinical isolates. CONCLUSION: Biofilm formation is a strain-dependent trait in C. auris, strongly associated with the type and phenotypic behaviour of the isolates. Colonising isolates of this fungus were found to be predominantly aggregative in nature, with a higher biofilm-forming capacity than non-aggregative ones.
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Aggregating strains demonstrate higher biofilm-forming capacity.
“Aggregation seems to play an important role in C. auris biofilm formation, since aggregating strains demonstrated higher biofilm-forming capacity, which was associated with distinct transcriptomic profiles compared to the non-aggregative strains (13, 23, 25, 57).”
From Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i> (2024) · cites this paper as [23]
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Conserved Role for Biofilm Matrix Polysaccharides in <i>Candida auris</i> Drug Resistance
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Candida auris: Epidemiology, biology, antifungal resistance, and virulence
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Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
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Rapid evolution of an adaptive multicellular morphology of Candida auris during systemic infection
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Mechanisms of pathogenicity for the emerging fungus Candida auris
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Ume6-dependent pathways of morphogenesis and biofilm formation in <i>Candida auris</i>
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- Authors
- Singh, R., Kaur, M., Chakrabarti, A., Shankarnarayan, S. A., & Rudramurthy, S. M.
- Affiliations
- Panjab University (IN)
- Post Graduate Institute of Medical Education and Research (IN)
- Corresponding author
- Singh, Rachna
- ORCID
- Venue
- Mycoses
- Publisher
- Wiley
- Published
- 2019-06-09
- Volume
- 62
- Issue
- 8
- Pages
- 706-709
- ISSN
- 0027-5557 (ISSN-L)
- 0933-7407
- 1439-0507
- OpenAlex
- W2947537849
- Cited by
- 86
- References
- 5
- Citations by year
- 2026: 5 · 2025: 15 · 2024: 14 · 2023: 20 · 2022: 6 · 2021: 8 · 2020: 14 · 2019: 4
- FWCI
- 6.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mycoses (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida auris, biofilm formation, fungal colonization, candidemia, cell aggregation, antifungal susceptibility, amphotericin B, fluconazole, nosocomial fungal infections, strain-dependent virulence
- MeSH terms
- Antifungal Agents, Candida, Humans, Microbial Sensitivity Tests, Virulence, Biofilms, Candidemia
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