The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Early weaning stress induces chronic functional diarrhea, intestinal barrier defects, and increased mast cell activity in a porcine model of early life adversity
Abstract
Background: Early life adversity ( ELA ) is a risk factor for development of gastrointestinal disorders later in life. The underlying mechanisms through which ELA and sex interact to influence disease susceptibility remains poorly understood. Methods: Utilizing a porcine early weaning stress ( EWS ) model to mimic ELA , we investigated the long‐term effects of EWS on functional diarrhea, ileal permeability, mast cell activity and mast cell relationship with enteric ganglia. Key Results: Juvenile and adult EWS pigs exhibited chronic, functional diarrhea ( EWS 43.6% vs late wean control( LWC ) 4.8%, P <.0001), increased intestinal permeability (2 fold increase EWS vs LWC , P <.0001), and mast cell numbers (at 7 weeks and 20 weeks ~1.6 fold increase EWS vs LWC , P <.05). Compared with EWS male castrates (Male‐C), females EWS pigs exhibited more frequent diarrhea (58.8% vs 29.9%, P =.0016), and increased intestinal permeability (1‐2 fold higher in EWS females, P <.001). Increased mast cell numbers and their enhanced co‐localization with neuronal ganglia were observed in both Male‐C and female EWS pigs; however, female pigs exhibited greater release of mast cell tryptase upon activation with c48/80 (~1.5 fold increase, P <.05), compared with Male‐C pigs. Conclusions and Inferences: These data demonstrate that pigs exposed to ELA exhibit increased vulnerability to functional diarrhea, intestinal permeability and mast cell activity. Further, these studies also showed that EWS female and Male‐C pigs exhibited dimorphic responses to EWS with female piglets exhibited greater susceptibility and severity of diarrhea, intestinal permeability and mast cell tryptase release. Together, these findings mimic some of the key pathophysiologic findings in human functional GI disorders functional gastrointestinal disorders ( FGID s) suggesting that the EWS porcine model could be a valuable preclinical translational model for FGID research associated with ELA .
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The combination of maternal separation and early weaning induces symptoms of IBS in pigs, and these correlate with increased numbers and activation of intestinal mast cells. 203”
From Mast cell ontogeny: From fetal development to life‐long health and disease (2023) · cites this paper as [203]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Intestinal mucosal barrier function in health and disease
Turner, J. R. (2009) · Nature reviews. Immunology · doi:10.1038/nri2653 · Cited by 3,952
Drossman, D. A., Li, Z., Andruzzi, E., Temple, R. D., Talley, N. J., Grant Thompson, W., Whitehead, W. E., Janssens, J., Funch-Jensen, P., Corazziari, E., et al. (1993) · Digestive Diseases and Sciences · doi:10.1007/bf01303162 · Cited by 2,198
Global Prevalence of and Risk Factors for Irritable Bowel Syndrome: A Meta-analysis
Lovell, R. M., & Ford, A. C. (2012) · Clinical Gastroenterology and Hepatology · doi:10.1016/j.cgh.2012.02.029 · Cited by 2,155
Barbara, G., Stanghellini, V., De Giorgio, R., Cremon, C., Cottrell, G. S., Santini, D., Pasquinelli, G., Morselli-Labate, A. M., Grady, E. F., Bunnett, N. W., et al. (2004) · Gastroenterology · doi:10.1053/j.gastro.2003.11.055 · Cited by 1,475
Clinical Characteristics of Women With a History of Childhood Abuse: Unhealed Wounds
McCauley, J. (1997) · JAMA · doi:10.1001/jama.1997.03540410040028 · Cited by 904
Piche, T., Barbara, G., Aubert, P., Bruley des Varannes, S., Dainese, R., Nano, J. L., Cremon, C., Stanghellini, V, De Giorgio, R., Galmiche, J. P., et al. (2009) · Gut · doi:10.1136/gut.2007.140806 · Cited by 540
Terminal ileal mucosal mast cells in irritable bowel syndrome
Weston, A. P., Biddle, W. L., Bhatia, P. S., & Miner, P. B. (1993) · Digestive Diseases and Sciences · doi:10.1007/bf01303164 · Cited by 338
Talley, N. J., Zinsmeister, A. R., & Melton, L. J. (1995) · American Journal of Epidemiology · doi:10.1093/oxfordjournals.aje.a117548 · Cited by 327
Martínez, C., Lobo, B., Pigrau, M., Ramos, L., González-Castro, A. M., Alonso, C., Guilarte, M., Guilá, M., de Torres, I., Azpiroz, F., et al. (2013) · Gut · doi:10.1136/gutjnl-2012-302093 · Cited by 283
Estradiol activates mast cells via a non-genomic estrogen receptor-α and calcium influx
Zaitsu, M., Narita, S.-I., Lambert, K. C., Grady, J. J., Estes, D. M., Curran, E. M., Brooks, E. G., Watson, C. S., Goldblum, R. M., & Midoro-Horiuti, T. (2007) · Molecular Immunology · doi:10.1016/j.molimm.2006.09.030 · Cited by 262
CRF induces intestinal epithelial barrier injury via the release of mast cell proteases and TNF-α
Overman, E. L., Rivier, J. E., & Moeser, A. J. (2012) · PLoS ONE · doi:10.1371/journal.pone.0039935 · Cited by 207
Self-citationPARK, J. H., RHEE, P., KIM, H. S., LEE, J. H., KIM, Y., KIM, J. J., & RHEE, J. C. (2006) · Journal of Gastroenterology and Hepatology · doi:10.1111/j.1440-1746.2005.04143.x · Cited by 196
Alonso, C., Guilarte, M., Vicario, M., Ramos, L., Ramadan, Z., Antolín, M., Martínez, C., Rezzi, S., Saperas, E., Kochhar, S., et al. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.03.036 · Cited by 128
Kanuri, N., Cassell, B., Bruce, S. E., White, K. S., Gott, B. M., Gyawali, C. P., & Sayuk, G. S. (2016) · Neurogastroenterology & Motility · doi:10.1111/nmo.12848 · Cited by 59
Research works printed but not linked: 47
- 10.1111/j.1572-0241.2007.01722.x · DOI · Paper page
- 10.1053/j.gastro.2005.09.071 · DOI
- 10.1016/j.cgh.2011.12.018 · DOI
- 10.1111/j.1572-0241.2006.00672.x · DOI · Paper page
- 10.1159/000333083 · DOI
- 10.1152/ajpgi.00168.2011 · DOI
- 10.1016/j.jpeds.2008.04.062 · DOI · Paper page
- 10.1111/apt.12829 · DOI
- 10.1053/j.gastro.2006.11.039 · DOI · Paper page
- 10.1038/ajg.2008.94 · DOI · Paper page
- 10.1111/apt.12080 · DOI
- 10.1053/j.gastro.2015.01.042 · DOI
- 10.3346/jkms.2003.18.2.204 · DOI
- 10.1152/ajpgi.00155.2012 · DOI
- 10.1136/gut.2010.213108 · DOI
- 10.1586/egh.10.88 · DOI
- 10.1152/ajpgi.00206.2015 · DOI
- 10.1152/ajpgi.00197.2006 · DOI
- 10.1152/ajpgi.00304.2006 · DOI
- 10.1152/ajpgi.00081.2009 · DOI
- 10.1111/nmo.12828 · DOI
- National Research Council (U.S.). Subcommittee on Swine Nutrition, 1998, Nutrient requirements of swine
- 10.1038/ajg.2014.181 · DOI
- 10.1097/MIB.0b013e3182802a4e · DOI
- Johnson DA, 2006, Human mast cell proteases: activity assays using thiobenzyl ester substrates, Methods Mol Biol, 315, 193
- 10.1186/1471-2172-9-45 · DOI
- 10.1038/ajg.2015.158 · DOI
- 10.1371/journal.pone.0059838 · DOI
- Barrett KE, 1991, Immune‐related intestinal chloride secretion. III. Acute and chronic effects of mast cell mediators on chloride secretion by a human colonic epithelial cell line, J Immunol, 147, 959, 10.4049/jimmunol.147.3.959 · DOI
- 10.1073/pnas.0906372106 · DOI
- 10.1111/j.1365-2036.2010.04409.x · DOI
- 10.1111/j.1365-2982.2007.00925.x · DOI
- 10.1016/j.coph.2009.06.022 · DOI
- 10.1186/s12876-014-0189-7 · DOI · Paper page
- 10.1111/j.1365-2982.2012.01928.x · DOI
- 10.1186/s13293-016-0113-7 · DOI
- 10.1111/nmo.12828 · DOI
- 10.1113/jphysiol.2009.169300 · DOI
- 10.1152/ajpgi.00274.2010 · DOI
- 10.1097/TA.0000000000000499 · DOI
- 10.1136/gut.2007.126680 · DOI
- 10.1371/journal.pone.0171617 · DOI
- 10.1111/j.1572-0241.2002.05913.x · DOI
- 10.1016/0016-5085(90)91023-Y · DOI
- 10.1111/j.1460-9568.2010.07511.x · DOI · Paper page
- 10.1016/j.expneurol.2014.01.021 · DOI
- 10.1111/j.1572-0241.2000.02314.x · DOI
Cited by
Sorted by citations
Moeser, A. J., Pohl, C. S., & Rajput, M. (2017) · Animal nutrition · doi:10.1016/j.aninu.2017.06.003 · Cited by 452
“Similar to human IBS, Pohl et al. (2017) demonstrated that adult pigs that were previously early weaned (16 d wean age) exhibited enhanced co-localization of intestinal mast cells with enteric nerves, compared with late weaned pigs (weaned at 28 d of age) (Pohl et al., 2017).”
BackgroundResultInfluentialMast cell ontogeny: From fetal development to life‐long health and disease
Chia, S. L., Kapoor, S., Carvalho, C., Bajénoff, M., & Gentek, R. (2023) · Immunological Reviews · doi:10.1111/imr.13191 · Cited by 60
Ziegler, A. L., & Blikslager, A. T. (2017) · Neurogastroenterology & Motility · doi:10.1111/nmo.13216 · Cited by 9
Metadata
- Authors
- Pohl, C. S., Medland, J. E., Mackey, E., Edwards, L. L., Bagley, K. D., DeWilde, M. P., Williams, K. J., & Moeser, A. J.
- Affiliations
- Department of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University East Lansing MI USA
- Department of Population Health and Pathobiology College of Veterinary Medicine North Carolina State University Raleigh NC USA
- Department of Pathobiology and Diagnostic Investigation College of Veterinary Medicine Michigan State University East Lansing MI USA
- ORCID
- Moeser, A. J.
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2017-06
- Volume
- 29
- Issue
- 11
- ISSN
- 1350-1925
- 1365-2982
- PMC
- PMC5650513
- Cited by
- 100
- References
- 14
- Language
- en
- Funders
- National Institutes of Health (HD072968, DK097462, R03 DK097462, OD011070)
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
