Efficacy and connectivity of intracolumnar pairs of layer 2/3 pyramidal cells in the barrel cortex of juvenile rats.
Open accessAbstract
Synaptically coupled layer 2/3 (L2/3) pyramidal neurones located above the same layer 4 barrel ('barrel-related') were investigated using dual whole-cell voltage recordings in acute slices of rat somatosensory cortex. Recordings were followed by reconstructions of biocytin-filled neurones. The onset latency of unitary EPSPs was 1.1 +/- 0.4 ms, the 20-80% rise time was 0.7 +/- 0.2 ms, the average amplitude was 1.0 +/- 0.7 mV and the decay time constant was 15.7 +/- 4.5 ms. The coefficient of variation (c.v.) of unitary EPSP amplitudes decreased with increasing EPSP peak and was 0.33 +/- 0.18. Bursts of APs in the presynaptic pyramidal cell resulted in EPSPs that, over a wide range of frequencies (5-100 Hz), displayed amplitude depression. Anatomically the barrel-related pyramidal cells in the lower half of layer 2/3 have a long apical dendrite with a small terminal tuft, while pyramidal cells in the upper half of layer 2/3 have shorter and often more 'irregularly' shaped apical dendrites that branch profusely in layer 1. The number of putative excitatory synaptic contacts established by the axonal collaterals of a L2/3 pyramidal cell with a postsynaptic pyramidal cell in the same column varied between 2 and 4, with an average of 2.8 +/- 0.7 (n = 8 pairs). Synaptic contacts were established predominantly on the basal dendrites at a mean geometric distance of 91 +/- 47 mum from the pyramidal cell soma. L2/3-to-L2/3 connections formed a blob-like innervation domain containing 2.8 mm of the presynaptic axon collaterals with a bouton density of 0.3 boutons per mum axon. Within the supragranular layers of its home column a single L2/3 pyramidal cell established about 900 boutons suggesting that 270 pyramidal cells in layer 2/3 are innervated by an individual pyramidal cell. In turn, a single pyramidal cell received synaptic inputs from 270 other L2/3 pyramidal cells. The innervation domain of L2/3-to-L2/3 connections superimposes almost exactly with that of L4-to-L2/3 connections. This suggests that synchronous feed-forward excitation of L2/3 pyramidal cells arriving from layer 4 could be potentially amplified in layer 2/3 by feedback excitation within a column and then relayed to the neighbouring columns.
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Horizontal, trans-columnar connections target other layer 2/3 pyramidal cells and correspond to the sequence memory circuits described above.
“The horizontal, trans-columnar connections target other layer 2/3 pyramidal cells [52],[53] and correspond to the sequence memory circuits that were described above.”
From Towards a mathematical theory of cortical micro-circuits (2009) · cites this paper as [52]
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- Authors
- Feldmeyer, D., Lübke, J., & Sakmann, B.
- Affiliations
- Forschungszentrum Jülich (DE)
- University of Freiburg (DE)
- Max Planck Institute for Medical Research (DE)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2006-08-24
- Volume
- 575
- Issue
- 2
- Pages
- 583-602
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC1819447
- OpenAlex
- W2124727398
- Cited by
- 314
- References
- 56
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 20 · 2023: 15 · 2022: 13 · 2021: 20 · 2020: 12 · 2019: 15 · 2018: 15 · 2017: 14 · 2016: 8 · 2015: 26 · 2014: 15 · 2013: 15 · 2012: 16
- FWCI
- 5.56
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- JuSER (Forschungszentrum Jülich) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Volkswagen Foundation
- Bundesministerium für Bildung und Forschung
- Max-Planck-Gesellschaft
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- layer 2/3 pyramidal cells, barrel cortex, axon collaterals, synaptic efficacy, synaptic depression, dendritic morphology, apical dendrites, somatosensory cortex, young rats, synaptic plasticity, synaptic facilitation
- MeSH terms
- Action Potentials, Animals, Animals, Newborn, Axons, Cell Communication, Dendrites, Synaptic Transmission, Neurons, Somatosensory Cortex, Synapses, Time Factors, Rats, Wistar, Pyramidal Cells, Excitatory Postsynaptic Potentials, Rats
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