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Cited by 222Metal and Thin Film MechanicsBone health and osteoporosis researchCellular Mechanics and Interactions

An Application of Nanoindentation Technique to Measure Bone Tissue Lamellae Properties

Hoffler, C. Edward, Guo, X. Edward, Zysset, Philippe K., Goldstein, Steven A.·2005·Journal of Biomechanical Engineering
DOI 10.1115/1.2073671
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Abstract

Measuring the microscopic mechanical properties of bone tissue is important in support of understanding the etiology and pathogenesis of many bone diseases. Knowledge about these properties provides a context for estimating the local mechanical environment of bone related cells that coordinate the adaptation to loads experienced at the whole organ level. The objective of this study was to determine the effects of experimental testing parameters on nanoindentation measures of lamellar-level bone mechanical properties. Specifically, we examined the effect of specimen preparation condition, indentation depth, repetitive loading, time delay, and displacement rate. The nanoindentation experiments produced measures of lamellar elastic moduli for human cortical bone (average value of 17.7±4.0GPa for osteons and 19.3±4.7GPa for interstitial bone tissue). In addition, the hardness measurements produced results consistent with data in the literature (average 0.52±0.15GPa for osteons and 0.59±0.20GPa for interstitial bone tissue). Consistent modulus values can be obtained from a 500-nm-deep indent. The results also indicated that the moduli and hardnesses of the dry specimens are significantly greater (22.6% and 56.9%, respectively) than those of the wet and wet and embedded specimens. The latter two groups were not different. The moduli obtained at a 5‐nm∕s loading rate were significantly lower than the values at the 10- and 20‐nm∕s loading rates while the 10- and 20‐nm∕s rates were not significantly different. The hardness measurements showed similar rate-dependent results. The preliminary results indicated that interstitial bone tissue has significantly higher modulus and hardness than osteonal bone tissue. In addition, a significant correlation between hardness and elastic modulus was observed.

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  • The difference between nanoscale moduli and those at greater length scales may be due to most nanoindentation measurements on bone being conducted under dry conditions.

    “Although the nanoscale moduli appear higher than those at greater length scales (Table 1), this difference may be due to the fact that most nanoindentation measurements on bone are conducted under dry conditions (38).”

    From Bone Mechanical Properties in Healthy and Diseased States (2018) · cites this paper as [38]

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Authors
Hoffler, C. E., Guo, X. E., Zysset, P. K., & Goldstein, S. A.
Affiliations
  • University of Michigan (US)
  • Orthopaedic Research Laboratories, Orthopaedic Surgery, The University of Michigan, Ann Arbor, MI 48109-0486
ORCID
Venue
Journal of Biomechanical Engineering
Publisher
ASME International
Published
2005-08-01
Volume
127
Issue
7
Pages
1046-1053
ISSN
  • 0148-0731 (ISSN-L)
  • 1528-8951
DOI
10.1115/1.2073671
OpenAlex
W1999426010
Cited by
222
References
61
Citations by year
2026: 3 · 2025: 11 · 2024: 7 · 2023: 11 · 2022: 11 · 2021: 4 · 2020: 12 · 2019: 15 · 2018: 12 · 2017: 9 · 2016: 12 · 2015: 8 · 2014: 15 · 2013: 15 · 2012: 15
FWCI
11.23
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Biomechanical Engineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ar034399-12, 1f32ar008338-01a1)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR34399, AR08338)
Topics
  • Physical Sciences › Engineering › Mechanics of Materials › Metal and Thin Film Mechanics
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
nanoindentation, bone lamellae, cortical bone, elastic modulus, hardness, osteons, specimen preparation, indentation depth, loading rate, bone mechanical properties
MeSH terms
Aged, Aged, 80 and over, Bone and Bones, Cadaver, Elasticity, Female, Hardness, Hardness Tests, Humans, Male, Middle Aged, Physical Stimulation, Sensitivity and Specificity, Stress, Mechanical, Reproducibility of Results, Weight-Bearing, Anisotropy, Compressive Strength, Nanotechnology, In Vitro Techniques

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