Cited by 712MicroRNA in disease regulationRNA and protein synthesis mechanismsRNA Research and Splicing

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Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish

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Bazzini, Ariel A., Lee, Miler T., Giraldez, Antonio J.·2012·Science

DOI 10.1126/science.1215704PMC PMC3547538

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Abstract

Translation Block: MicroRNAs (miRNAs) are small, noncoding RNA genes that are found in the genomes of most eukaryotes, where they play an important role in the regulation of gene expression. Although whether gene activity is repressed by blocking translation of messenger RNA (mRNA) targets, or by promoting their deadenylation and then degradation, has been open to debate. Bazzini et al. (p. 233 , published online 15 March) and Djuranovic et al. (p. 237 ) looked at early points in the repression reaction in the zebrafish embryo or in Drosophila tissue culture cells, respectively, and found that translation was blocked before target mRNAs were significantly deadenylated and degraded. Thus, miRNAs appear to interfere with the initiation step of translation.

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Self-citation
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Background
The proteome under translational control
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BackgroundMethod
Rules for functional microRNA targeting
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Background
microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development
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Background
Translation efficiency is a determinant of the magnitude of miRNA-mediated repression
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Background
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“translational repression and mRNA decay, others stating decay occurs cotranslationally, and still others stating silencing and decay can be uncoupled (Bazzini et al., 2012; Béthune et al., 2012; Bose et al., 2017; Djuranovic et al., 2012; Eichhorn et al., 2014; Guo et al., 2010; Pillai et al., 2005; Subtelny et al., 2…”
Background
miR-96 is required for normal development of the auditory hindbrain
Schlüter, T., Berger, C., Rosengauer, E., Fieth, P., Krohs, C., Ushakov, K., Steel, K. P., Avraham, K. B., Hartmann, A. K., Felmy, F., et al. (2018) · Human Molecular Genetics · doi:10.1093/hmg/ddy007 · Cited by 36
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Background
Conserved mRNA-granule component Scd6 targets Dhh1 to repress translation initiation and activates Dcp2-mediated mRNA decay in vivo
Zeidan, Q., He, F., Zhang, F., Zhang, H., Jacobson, A., & Hinnebusch, A. G. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007806 · Cited by 39
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Background
Fishing forward and reverse: Advances in zebrafish phenomics
Fuentes, R., Letelier, J., Tajer, B. J., Valdivia, L. E., & Mullins, M. C. (2018) · Mechanisms of Development · doi:10.1016/j.mod.2018.08.007 · Cited by 38
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Background
Micro RNA expression profiling of the developing murine upper lip
Warner, D. R., Mukhopadhyay, P., Brock, G. N., Webb, C., Pisano, M., & Greene, R. M. (2014) · Development Growth & Differentiation · doi:10.1111/dgd.12140 · Cited by 35
“MiRNAs regulate expression of genes post-transcriptionally by binding to and then inhibiting the translation of, and/or destabilizing, their target mRNAs (Bartel 2009; Bazzini et al. 2012; Djuranovic et al. 2012).”
Background
Detecting translational regulation by change point analysis of ribosome profiling data sets
Županič, A., Méplan, C., Grellscheid, S. N., Mathers, J. C., Kirkwood, T. B. L., Hesketh, J. E., & Shanley, D. P. (2014) · RNA · doi:10.1261/rna.045286.114 · Cited by 34
“Several studies have used Ribo-Seq to study translational regulation after different interventions, and most have used translational efficiency of groups of transcripts as their measure of interest (Ingolia et al. 2011; Bazzini et al. 2012).”
GW182-Free microRNA Silencing Complex Controls Post-transcriptional Gene Expression during Caenorhabditis elegans Embryogenesis
Jannot, G., Michaud, P., Huberdeau, M. Q., Morel-Berryman, L., Brackbill, J. A., Piquet, S., McJunkin, K., Nakanishi, K., & Simard, M. J. (2016) · PLoS Genetics · doi:10.1371/journal.pgen.1006484 · Cited by 35
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Background
Elevated MMP10/13 mediated barrier disruption and NF-κB activation aggravate colitis and colon tumorigenesis in both individual or full miR-148/152 family knockout mice
Tang, K., Wu, Z., Sun, M., Huang, X., Sun, J., Shi, J., Wang, X., Miao, Z., Gao, P., Song, Y., et al. (2022) · Cancer Letters · doi:10.1016/j.canlet.2021.12.033 · Cited by 35
Short poly(A) tails are protected from deadenylation by the LARP1–PABP complex
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Background
The combination of transcriptomics and informatics identifies pathways targeted by miR-204 during neurogenesis and axon guidance
Conte, I., Merella, S., García-Manteiga, J. M., Migliore, C., Lazarević, D., Carrella, S., Marco-Ferreres, R., Avellino, R., Davidson, N. P., Emmett, W., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku498 · Cited by 33
“Recent studies have demonstrated the importance of translation inhibition as a mode of microRNA targeting (47,48), which cannot be addressed through standard RNA-Seq approaches, but require ribosome profiling, which was beyond the scope of this study.”
Background
Robust partitioning of microRNA targets from downstream regulatory changes
Patel, R. K., West, J. D., Jiang, Y., Fogarty, E. A., & Grimson, A. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa687 · Cited by 34
“Therefore, CARP is well-suited for the identification of true miRNA targets, except for the rare contexts such as the Zebrafish embryo (64,65) where miRNA direct targets are subjected exclusively to translational repression without”
Background
miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development
Olena, A. F., Rao, M. B., Thatcher, E. J., Wu, S.-Y., & Patton, J. G. (2015) · Developmental Biology · doi:10.1016/j.ydbio.2015.01.016 · Cited by 32
“This results in the recruitment of the RNA Induced Silencing Complex (RISC), the effector complex that mediates translation repression, deadenylation, and decay of target mRNAs (Bazzini et al., 2012; Djuranovic et al., 2012; Giraldez et al., 2006).”
Background
RNA sequencing: from tag-based profiling to resolving complete transcript structure
de Klerk, E., den Dunnen, J. T., & Hoen, P. A. C. ’. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1637-9 · Cited by 35
“A gene may be transcribed into (1) an alternatively spliced variant targeted for nonsense-mediated decay [9], or (2) an alternatively polyadenylated variant which has gained or lost regulatory sequences recognized by stabilizing RNA-binding proteins or microRNAs [10–13].”
Background
Small Interfering RNA–Mediated Translation Repression Alters Ribosome Sensitivity to Inhibition by Cycloheximide inChlamydomonas reinhardtii
Ma, X., Kim, E., Kook, I., Ma, F., Voshall, A., Moriyama, E. N., & Cerutti, H. D. (2013) · The Plant Cell · doi:10.1105/tpc.113.109256 · Cited by 35
“…timing of different events suggest that miRNA targets in Danio rerio, Drosophila, and HeLa cell lines are first subject to translational inhibition, followed by effects on deadenylation and mRNA degradation (Bazzini et al., 2012; Béthune et al., 2012; Djuranovic et al., 2012; Mishima et al., 2012).”
Influential
A Polysome-Based microRNA Screen Identifies miR-24-3p as a Novel Promigratory miRNA in Mesothelioma
Oliveto, S., Alfieri, R. R., Miluzio, A., Scagliola, A., Secli, R. S., Gasparini, P., Grosso, S., Cascione, L., Mutti, L., & Biffo, S. (2018) · Cancer Research · doi:10.1158/0008-5472.can-18-0655 · Cited by 33
“We exploited the well-known observation that miRNA translational repressionofmRNA targets is followedby their degradation (4, 28).”
Background
MicroRNA governs bistable cell differentiation and lineage segregation via a noncanonical feedback
Li, C.-J., Liau, E. S., Lee, Y., Huang, Y., Liu, Z., Willems, A. R., Garside, V. C., McGlinn, E., Chen, J., & Hong, T. (2021) · Molecular Systems Biology · doi:10.15252/msb.20209945 · Cited by 28
“…limitation of using signed directed graphs to describe GRNs. mRNA–miRNA feedback and the diverse molecular functions of miRNA The canonical molecular functions of miRNA include mRNA destabilization and translational inhibition (Petersen et al, 2006; Bazzini et al, 2012; Djuranovic et al, 2012).”
Background
Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization
Gillen, S. L., Giacomelli, C., Hodge, K., Zanivan, S. R., Bushell, M., & Wilczynska, A. (2021) · Genome biology · doi:10.1186/s13059-021-02494-w · Cited by 34
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MiR-125b-5p Is Involved in Sorafenib Resistance through Ataxin-1-Mediated Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma
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Transcriptome dynamics and diversity in the early zebrafish embryo
Aanes, H., Collas, P., & Aleström, P. (2013) · Briefings in Functional Genomics · doi:10.1093/bfgp/elt049 · Cited by 30
“RNA-seq of ribosome protected RNAs from wildtype and maternal and zygotic dicer (MZdicer) mutant embryos revealed no difference in ribosome bound mRNAs between the two groups pre-ZGA [23].”
BackgroundInfluential
MicroRNA miR-181—A Rheostat for TCR Signaling in Thymic Selection and Peripheral T-Cell Function
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“The rules governing the choice of repressive mechanism remain unclear, but both processes may also be kinetically linked [24].”
Background
Genomic analysis of RNA localization
Taliaferro, J. M., Wang, E. T., & Burge, C. B. (2014) · RNA Biology · doi:10.4161/rna.32146 · Cited by 32
Ribosome Profiling: A Tool for Quantitative Evaluation of Dynamics in mRNA Translation
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Identification of long non-coding transcripts with feature selection: a comparative study
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“We collected the zebrafish ribo-seq profile provided by GWIPS-viz database, which is an aggregate of two ribo-seq studies [70, 71], and the mRNA-seq profile provided by Pauli et al. [21].”
Method
Translation Initiation Regulated by RNA-Binding Protein in Mammals: The Modulation of Translation Initiation Complex by Trans-Acting Factors
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Critical contribution of 3′ non-seed base pairing to the in vivo function of the evolutionarily conserved let-7a microRNA
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“miRNA-mediated gene regulation can occur via translational repression or mRNA destabilization, indicated by decreased translational efficiency (TE) or reduced mRNA abundance, respectively (Bazzini et al., 2012; Giraldez et al., 2006).”
Background
MicroRNAs in vascular tissue engineering and post-ischemic neovascularization
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“This interaction usually leads to translational inhibition, deadenylation and subsequent RNA degradation [11].”
Background
Nanog safeguards early embryogenesis against global activation of maternal β-catenin activity by interfering with TCF factors
He, M., Zhang, R., Zhang, F., Jiao, S., Ding, Y., Wang, H., & Sun, Y. (2020) · PLoS Biology · doi:10.1371/journal.pbio.3000561 · Cited by 27
“On the other hand, the expression of sod1, a 342 maternal mRNA targeted by miR-430 [58], was accumulated in nanog 343 morphants and the En-Nanog overexpressed embryos, indicating the defects 344 of maternal mRNA clearance (Fig.”
Functional viromic screens uncover regulatory RNA elements
Seo, J. J., Jung, S. J., Yang, J.-H., Choi, D.-E., & Kim, V. N. (2023) · Cell · doi:10.1016/j.cell.2023.06.007 · Cited by 29
Salidroside induces neuronal differentiation of mouse mesenchymal stem cells through Notch and BMP signaling pathways
Zhao, H., Qi, S.-N., Dong, J., Ha, X., Li, X., Zhang, Q., Yang, Y., Bai, J., & Zhao, L. (2014) · Food and Chemical Toxicology · doi:10.1016/j.fct.2014.05.031 · Cited by 27
Cap homeostasis is independent of poly(A) tail length
Kiss, D., Oman, K. M., Dougherty, J. A., Mukherjee, C., Bundschuh, R. A., & Schoenberg, D. R. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv1460 · Cited by 28
“Poly(A) tail length on individual transcripts was determined by GI tailing using a modification of the approach in (23).”
Method
Using microRNA as an Alternative Treatment for Hyperlipidemia and Cardiovascular Disease: cardio-miRs in the Pipeline
Hennessy, E., & Moore, K. (2013) · Journal of Cardiovascular Pharmacology · doi:10.1097/fjc.0b013e31829d48bf · Cited by 32
“However, subsequent studies using Drosophila melanogaster and zebra fish as models demonstrated that the effects of miRNAs on translational repression precede the effects on mRNA target deadenylation or decay.(30,31)”
Result
Reduced Expression of Ribosomal Proteins Relieves MicroRNA-Mediated Repression
Janas, M. M., Wang, E. T., Love, T. M., Harris, A., Stevenson, K. E., Semmelmann, K., Shaffer, J. M., Chen, P., Doench, J. G., Subrahmanyam, Y. V. B. K., et al. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.04.008 · Cited by 27
“More recent ribosome footprinting analysis at earlier time points demonstrated that repression of translation initiation precedes deadenylation for the majority of miR-430-targeted mRNAs in zebrafish and that mRNA decay is likely a consequence of these earlier activities (Bazzini et al., 2012).”
Background
A tale of two sequences: microRNA-target chimeric reads
Broughton, J. P., & Pasquinelli, A. E. (2016) · Genetics Selection Evolution · doi:10.1186/s12711-016-0209-x · Cited by 27
“Techniques applied to the identification of miRNA targets include stable isotope labeling by amino acids in cell culture (SILAC) [17–19] and ribosome profiling [20, 21].”
Method
Genome-Wide Posttranscriptional Dysregulation by MicroRNAs in Human Asthma as Revealed by Frac-seq
Martínez-Nuñez, R. T., Rupani, H., Platé, M., Niranjan, M., Chambers, R. C., Howarth, P. H., & Sánchez-Elsner, T. (2018) · The Journal of Immunology · doi:10.4049/jimmunol.1701798 · Cited by 28
“results are also consequent with microRNAs affecting both mRNA degradation (4, 24) and translation inhibition (2, 5).”
Background
Role of microRNAs in the Development of Cardiovascular Disease in Systemic Autoimmune Disorders
López-Pedrera, C., Barbarroja, N., Patiño-Trives, A. M., Luque-Tévar, M., Torres-Granados, C., Aguirre, M. A., Collantes‐Estévez, E., & Pérez‐Sánchez, C. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21062012 · Cited by 28
MicroRNA-24-3p regulates neuronal differentiation by controlling hippocalcin expression
Kang, M., Park, S., & Han, J. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03290-3 · Cited by 27
Proteolytic Restriction of Chordin Range Underlies BMP Gradient Formation
Tuazon, F. B., Wang, X., Andrade, J. L., Umulis, D. M., & Mullins, M. C. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108039 · Cited by 27
Plant bioactive compounds driven microRNAs (miRNAs): A potential source and novel strategy targeting gene and cancer therapeutics
Sahreen, S., Vijayarathna, S., Hemagirri, M., Adnan, M., Hassan, M. I., Patel, M. B., Gupta, R., Shanmugapriya, S., Chen, Y., Gopinath, S. C. B., et al. (2024) · Non-coding RNA Research · doi:10.1016/j.ncrna.2024.06.003 · Cited by 27
“Nevertheless, target mRNA degradation does not seem the only way miRNA-dependent gene silencing works [183,184].”
Background
Divergent MicroRNA Targetomes of Closely Related Circulating Strains of a Polyomavirus
Chen, C., Cox, J. E., Kincaid, R. P., Martinez, A., & Sullivan, C. S. (2013) · Journal of Virology · doi:10.1128/jvi.01711-13 · Cited by 26
Translating Extracellular Microrna into Clinical Biomarkers for Drug-Induced Toxicity: from High-Throughput Profiling to Validation
Wang, W., Shi, Q., Mattes, W. B., Mendrick, D. L., & Yang, X. (2015) · Biomarkers in Medicine · doi:10.2217/bmm.15.86 · Cited by 26
The Role of MicroRNAs in Development and Function of Regulatory T Cells – Lessons for a Better Understanding of MicroRNA Biology
Kunze‐Schumacher, H., & Krueger, A. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.02185 · Cited by 26
“However, both mechanisms may also be kinetically linked (19).”
Background
Role of microRNAs in Semaphorin function and neural circuit formation
Baudet, M., Bellon, A., & Holt, C. E. (2012) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2012.11.004 · Cited by 26
miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1
Poon, V. Y., Gu, M., Ji, F., VanDongen, A. M. J., & Fivaz, M. L. (2016) · BMC Genomics · doi:10.1186/s12864-016-3139-7 · Cited by 25
Small non-coding RNAs-based bone regulation and targeting therapeutic strategies
Yang, Y., & Fang, S. (2016) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2016.11.018 · Cited by 26
“…target mRNAs through complementary base pairing to different sites in the 3’-untranslated regions, and they exert their biological functions through translational repression and/or being followed by deadenylation and degradation of their mRNA targets (Bazzini et al., 2012; Djuranovic et al., 2012).”
Background
Biomarkers Associated with Atrial Fibrosis and Remodeling
Dilaveris, P., Antoniou, C. K., Manolakou, P., Tsiamis, E. G., Gatzoulis, K. A., & Tousoulis, D. (2018) · Current Medicinal Chemistry · doi:10.2174/0929867324666170918122502 · Cited by 26
Intracytoplasmic Re-localization of miRISC Complexes
Akgül, B., & Erdoğan, İ. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00403 · Cited by 26
Genome‐wide identification of microRNAs regulating the human prion protein
Pease, D., Scheckel, C., Schaper, E., Eckhardt, V., Emmenegger, M., Xénarios, I., & Aguzzi, A. (2018) · Brain Pathology · doi:10.1111/bpa.12679 · Cited by 24
“RNA was extracted at the same 72 h time point at which we measured PrPC protein levels, as miRNA-target interactions often initially result in translational repression followed by mRNA degradation (6).”
Method
Temporospatial guidance of activity-dependent gene expression by microRNA: mechanisms and functional implications for neural plasticity
Kiltschewskij, D. J., & Cairns, M. J. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky1235 · Cited by 26
“(56) additionally incorporated ribosome profiling to show that miR-430 induces translational repression prior to target degradation in zebrafish embryos, again further obscuring the functional role of miRNA regulation of translation.”
Background
Cis to trans: small ORF functions emerging through evolution
Baena-Angulo, C., Platero, A. I., & Couso, J. P. (2024) · Trends in Genetics · doi:10.1016/j.tig.2024.10.012 · Cited by 25
Altered Gene Expression Associated with microRNA Binding Site Polymorphisms
Võsa, U., Esko, T., Kasela, S., & Annilo, T. (2015) · PLoS ONE · doi:10.1371/journal.pone.0141351 · Cited by 26
“LU /Q P /W E [4 5]”
BackgroundInfluential
Acute kidney injury: a paradigm for miRNA regulation of the cell cycle
Khalid, U., Bowen, T., Fraser, D. J., & Jenkins, R. L. (2014) · Biochemical Society Transactions · doi:10.1042/bst20140093 · Cited by 25
Comparative Analysis of microRNA Binding Site Distribution and microRNA-Mediated Gene Expression Repression of Oncogenes and Tumor Suppressor Genes
Tian, S., Wang, J., Zhang, F., & Wang, D. (2022) · Genes · doi:10.3390/genes13030481 · Cited by 23
The molecular mechanisms underpinning maternal mRNA dormancy
Lorenzo‐Orts, L., & Pauli, A. (2024) · Biochemical Society Transactions · doi:10.1042/bst20231122 · Cited by 28
The microRNAs in an Ancient Protist Repress the Variant-Specific Surface Protein Expression by Targeting the Entire Coding Sequence
Saraiya, A. A., Li, W., Jesse, W., Chang, C. H., & Wang, C. C. (2014) · PLoS Pathogens · doi:10.1371/journal.ppat.1003791 · Cited by 25
T-cells require post-transcriptional regulation for accurate immune responses
Salerno, F., & Wolkers, M. C. (2015) · Biochemical Society Transactions · doi:10.1042/bst20150154 · Cited by 24
Improving microRNA target prediction with gene expression profiles
Ovando‐Vázquez, C., Lepe-Soltero, D., & Abreu‐Goodger, C. (2016) · BMC Genomics · doi:10.1186/s12864-016-2695-1 · Cited by 26
“We selected another zebrafish experiment, this time using high-throughput sequencing to measure expression [2].”
BackgroundMethodInfluential
Literature review of baseline information to support the risk assessment of RNAi‐based GM plants
Pačes, J., Nič, M., Novotny, T., & Svoboda, P. (2017) · EFSA Supporting Publications · doi:10.2903/sp.efsa.2017.en-1246 · Cited by 27
Implications of MicroRNAs in Oncolytic Virotherapy
Ros, X. B., Rovira-Rigau, M., & Fillat, C. (2017) · Frontiers in Oncology · doi:10.3389/fonc.2017.00142 · Cited by 27
“In consequence, the repression of miRNAs target genes can be evidenced by depletion of the mRNA content (10, 11).”
Background
AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke
Kobayashi, M., Benakis, C., Anderson, C. J., Moore, M. J., Poon, C., Uekawa, K., Dyke, J. P., Fak, J. J., Mele, A., Park, C. Y., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.06.075 · Cited by 25
A single-nucleotide resolution capillary gel electrophoresis workflow for poly(A) tail characterization in the development of mRNA therapeutics and vaccines
Grandi, D. D., Dayeh, D. M., Kaur, K., Chen, Y., Henderson, S., Moon, Y., Bhowmick, A., Ihnat, P. M., Fu, Y., Muthusamy, K., et al. (2023) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2023.115692 · Cited by 23
miR-430 regulates zygotic mRNA during zebrafish embryogenesis
Amaral, D. B., Egidy, R., Perera, A. G., & Bazzini, A. (2024) · Genome biology · doi:10.1186/s13059-024-03197-8 · Cited by 23
“Fourth, previously reported miR-430 targets showed higher total and unlabeled (Maternal) mRNA fold changes in miR430-LNA with respect to the LNA-control embryos compared to a control group of genes lacking miR-430 target site [40]; Fig.”
BackgroundInfluentialSelf-citation
Computational Prediction of microRNA Targets
Laganà, A. (2015) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-22380-3_12 · Cited by 24
MicroTrout: A comprehensive, genome-wide miRNA target prediction framework for rainbow trout, Oncorhynchus mykiss
Mennigen, J. A., & Zhang, D. (2016) · Comparative Biochemistry and Physiology Part D Genomics and Proteomics · doi:10.1016/j.cbd.2016.07.002 · Cited by 24
“Since biological function of miRNAs is mediated at the mRNA and protein level, through the miRNAs action of blocking translation and decreasing target mRNA stability (Bazzini et al., 2012), the annotated mRNA coding sequences (Supplemental File B) linked to their respective 3’UTRs (Supplemental File C) by matching seq…”
Background
Integrated Transcriptomic and Translatomic Inquiry of the Role of Betaine on Lipid Metabolic Dysregulation Induced by a High-Fat Diet
Huang, T., Yu, L., Pan, H., Ma, Z., Wu, T., Zhang, L., Liu, K., Qi, Q., Miao, W., Song, Z., et al. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.751436 · Cited by 24
“Ribo-seq enables gene identification and quantification at the genomic translational level, which has been widely used in different species including human (19), mouse (20), zebrafish (21), drosophila (22), rice (23), and maize (24).”
Background
Microtubule-associated protein 9 (Map9/Asap) is required for the early steps of zebrafish development
Fontenille, L., Rouquier, S., Lutfalla, G., & Giorgi, D. (2014) · Cell Cycle · doi:10.4161/cc.27944 · Cited by 24
Personalized Medicine: Applying ‘omics’ to Lung Fibrosis
Herazo‐Maya, J. D., & Kaminski, N. (2012) · Biomarkers in Medicine · doi:10.2217/bmm.12.38 · Cited by 24
MicroRNAs, HIV and HCV: a complex relation towards pathology
Piedade, D., & Azevedo‐Pereira, J. M. (2016) · Reviews in Medical Virology · doi:10.1002/rmv.1881 · Cited by 24
The effects of a MAP2K5 microRNA target site SNP on risk for anxiety and depressive disorders
Jensen, K. P., Kranzler, H. R., Stein, M. B., & Gelernter, J. (2014) · American Journal of Medical Genetics Part B: Neuropsychiatric Genetics · doi:10.1002/ajmg.b.32219 · Cited by 25
“MicroRNAs interact with mRNA by base pairing, primarily with the 30UTR, and function to reduce protein expression [Guo et al., 2010; Bazzini et al., 2012].”
Background
Trip to ER
Ma, X., Cao, X., Mo, B., & Chen, X. (2013) · RNA Biology · doi:10.4161/rna.26313 · Cited by 22
“However, using zebrafish embryos as well as Drosophila and human cultured cells, recent studies examined the two effects of miRNAs (mRNA decay and translational repression) with temporal resolution and revealed that miRNAs first cause translational repression, which may be followed by deadenylation and decay of the tr…”
Method
Weak 5′-mRNA Secondary Structures in Short Eukaryotic Genes
Ding, Y., Shah, P., & Plotkin, J. B. (2012) · Genome Biology and Evolution · doi:10.1093/gbe/evs082 · Cited by 24
“Key words: translation initiation, ribosome density, codon bias, gene length.”
BackgroundResult
Single‐cell mRNA profiling reveals the hierarchical response of miRNA targets to miRNA induction
Rzepiela, A. J., Viña‐Vilaseca, A., Breda, J., Ghosh, S., Syed, A. P., Gruber, A. J., Grandy, W. A., Eschbach, K., Beisel, C., Nimwegen, E. V., et al. (2018) · Molecular Systems Biology · doi:10.15252/msb.20188266 · Cited by 24
“However, time series of mRNA and protein-level measurements after miRNA transfection also revealed that repression of target translation precedes the increase in its degradation rate (Bazzini et al, 2012; Hausser et al, 2013; Eichhorn et al, 2014).”
Background
Modeling neurodevelopmental disorder-associated human AGO1 mutations in Caenorhabditis elegans Argonaute alg-1
Duan, Y., Li, L., Panzade, G. P., Piton, A., Zinovyeva, A., & Ambros, V. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2308255121 · Cited by 22
“NEXTflex Small RNA Library Prep kit v3 (PerkinElmer, cat# NOVA-5132) was used to prepare libraries according to the manufacturer’s instructions, followed by size selection of final PCR products as previously described(6).”
Method

Authors: Bazzini, A. A., Lee, M. T., & Giraldez, A. J.
Affiliations: Yale University (US)
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
Corresponding author: Bazzini, Ariel A.
Lee, Miler T.
ORCID: Bazzini, Ariel A.
Lee, Miler T.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2012-04-13
Volume: 336
Issue: 6078
Pages: 233-237
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1215704
PMC: PMC3547538
OpenAlex: W2026570758
Cited by: 709
References: 30
Citations by year: 2026: 11 · 2025: 22 · 2024: 30 · 2023: 19 · 2022: 31 · 2021: 43 · 2020: 33 · 2019: 34 · 2018: 67 · 2017: 70 · 2016: 59 · 2015: 54 · 2014: 81 · 2013: 97 · 2012: 56
FWCI: 30.58
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute of General Medical Sciences (R01GM081602, R01GM102251)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (F32HD071697)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: miR-430, microRNA, protein synthesis inhibition, mRNA degradation, deadenylation, ribosome profiling, ribosome occupancy, translation initiation, Dicer, zebrafish embryos, embryonic development
MeSH terms: Animals, Embryo, Nonmammalian, Peptide Chain Initiation, Translational, Poly A, Ribosomes, RNA, Messenger, Protein Biosynthesis, Zebrafish, Gene Expression Regulation, Developmental, 3' Untranslated Regions, RNA Stability, Zebrafish Proteins, MicroRNAs, Ribonuclease III, Embryonic Development

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Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish

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