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Cited by 1,104

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Wound Macrophages Express TGF-α and Other Growth Factors in Vivo: Analysis by mRNA Phenotyping

Rappolee, Daniel A., Mark, David, Banda, Michael J., Werb, Zena·1988·Science
DOI 10.1126/science.3041594
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Abstract

The presence of macrophages is required for the regeneration of many cell types during wound healing. Macrophages have been reported to express a wide range of mitogenic factors and cytokines, but none of these factors has been shown in vivo to sustain all the wound-healing processes. It has been suggested that transforming growth factor-α (TGF-α) may mediate angiogenesis, epidermal regrowth, and formation of granulation tissue in vivo. Macrophages isolated from a wound site, and not exposed to cell culture conditions, expressed messenger RNA transcripts for TGF-α, TGF-β, platelet-derived growth factor A-chain, and insulin-like growth factor-1. The expression of these transcripts was determined by a novel method for RNA analysis in which low numbers of mouse macrophages were isolated from wound cylinders, their RNA was purified and reverse-transcribed, and the complementary DNA was amplified in a polymerase chain reaction primed with growth factor sequence-specific primers. This single-cell RNA phenotyping procedure is rapid and has the potential for quantification, and mRNA transcripts from a single cell or a few cells can be unambiguously demonstrated, with the simultaneous analysis of several mRNA species. Macrophages from wounds expressed TGF-α antigen, and wound fluids contained TGF-α. Elicited macrophages in culture also expressed TGF-α transcripts and polypeptide in a time-dependent manner after stimulation with modified low-density lipoproteins and lipopolysaccharide endotoxin, which are characteristic of the activators found in injured tissues.

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Metadata

Authors
Rappolee, D. A., Mark, D., Banda, M. J., & Werb, Z.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1988-08-05
Volume
241
Issue
4866
Pages
708-712
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.3041594
OpenAlex
W1970516412
Cited by
1,104
References
2
FWCI
142.66
Type
journal article
Language
en
Open access
closed

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