Engineered CRISPR-Cas9 nuclease with expanded targeting space

Nishimasu, Hiroshi, Shi, Xi, Ishiguro, Soh, Gao, Linyi, Hirano, Seiichi, Okazaki, Sae, Noda, Taichi, Abudayyeh, Omar O. +13 more·2018·Science

DOI 10.1126/science.aas9129

Cited by (1,001)

Items 301–400 of 1,001

Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience
Muhammad Naeem Sattar, Zafar Iqbal, Jameel Mohammed Al-Khayri, Shri Mohan Jain · 2021 · Plants
Machine learning-coupled combinatorial mutagenesis enables resource-efficient engineering of CRISPR-Cas9 genome editor activities
Dawn Thean, Hoi Yee Chu, John H.C. Fong, Becky K.C. Chan, Peng Zhou, Cynthia C. S. Kwok, Yee Man Chan, Silvia Y. L. Mak, Gigi C.G. Choi, Joshua W. K. Ho et al. · 2022 · Nature Communications
Structure and engineering of the minimal type VI CRISPR-Cas13bt3
Ryoya Nakagawa, Soumya Kannan, Han Altae-Tran, Satoru N. Takeda, Atsuhiro Tomita, Hisato Hirano, Tsukasa Kusakizako, Tomohiro Nishizawa, Keitaro Yamashita, Feng Zhang et al. · 2022 · Molecular Cell
Altered DNA repair pathway engagement by engineered CRISPR-Cas9 nucleases
Vikash P. Chauhan, Phillip A. Sharp, Róbert Langer · 2023 · Proceedings of the National Academy of Sciences
Increasing intracellular dNTP levels improves prime editing efficiency
Pengpeng Liu, Karthikeyan Ponnienselvan, Thomas Nyalile, Sarah Oikemus, Anya T. Joynt, Sukanya Iyer, Karen L. Kelly, Dongsheng Guo, Pyae Phyo Kyawe, Emma Vanderleeden et al. · 2024 · Nature Biotechnology
Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations
Muna Alariqi, Mohamed Ramadan, Lu Yu, Fengjiao Hui, Amjad Hussain, Xiaofeng Zhou, Yu Yu, Xianlong Zhang, Shuangxia Jin · 2025 · Advanced Science
Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice
Ying Chieh Wu, Wen Xu, Feipeng Wang, Si Zhao, Feng Feng, Jinling Song, Chengwei Zhang, Jinxiao Yang · 2019 · Frontiers in Genetics
Cas Endonuclease Technology—A Quantum Leap in the Advancement of Barley and Wheat Genetic Engineering
Iris Koeppel, Christian W. Hertig, Robert E. Hoffie, Jochen Kumlehn · 2019 · International Journal of Molecular Sciences
Battling CRISPR-Cas9 off-target genome editing
Daisy Li, Hong Zhou, Xiao Zeng · 2019 · Cell Biology and Toxicology
A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants
Xiong, Xiangyu, Li, Zhenxiang, Liang, Jieping, Liu, Kehui, Li, Chenlong, Li, Jian-Feng · 2022 · Nucleic Acids Research
CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding
Changtian Pan, Yiping Qi · 2023 · Nature Protocols
Multiplexed assays of variant effect for clinical variant interpretation
Abbye E. McEwen, Malvika Tejura, Shawn Fayer, Lea M. Starita, Douglas M. Fowler · 2025 · Nature Reviews Genetics
Combined Computational–Experimental Approach to Explore the Molecular Mechanism of SaCas9 with a Broadened DNA Targeting Range
Binquan Luan, Guangxue Xu, Mei Feng, Le Cong, Ruhong Zhou · 2019 · Journal of the American Chemical Society
Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine
Kutubuddin A. Molla, Subhasis Karmakar, Tofazzal Islam · 2020 · Springer protocols handbooks/Springer protocols
Structure of the type V-C CRISPR-Cas effector enzyme
Nina Kurihara, Ryoya Nakagawa, Hisato Hirano, Sae Okazaki, Atsuhiro Tomita, Kan Kobayashi, Tsukasa Kusakizako, Tomohiro Nishizawa, Keitaro Yamashita, David A. Scott et al. · 2022 · Molecular Cell
The Design and Application of DNA-Editing Enzymes as Base Editors
Kartik Lakshmi Rallapalli, Alexis C. Komor · 2023 · Annual Review of Biochemistry
Elimination of CaMKIIδ Autophosphorylation by CRISPR-Cas9 Base Editing Improves Survival and Cardiac Function in Heart Failure in Mice
Simon Lebek, Xurde M. Caravia, Francesco Chemello, Wei Hong Tan, John McAnally, Kenian Chen, Lin Xu, Ning Liu, Rhonda Bassel‐Duby, Eric N. Olson · 2023 · Circulation
Engineered prime editors with minimal genomic errors
Vikash P. Chauhan, Phillip A. Sharp, Róbert Langer · 2025 · Nature
Pseudomonas putida in the quest of programmable chemistry
Esteban Martínez‐García, Vı́ctor de Lorenzo · 2019 · Current Opinion in Biotechnology
CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi
Mati Ullah, Lin Xia, Shangxian Xie, Su Sun · 2020 · Biotechnology and Applied Biochemistry
Base editing and prime editing in laboratory animals
Federico Caso, Benjamin Davies · 2021 · Laboratory Animals
A most formidable arsenal: genetic technologies for building a better mouse
James F. Clark, Colin J. Dinsmore, Philippe M. Soriano · 2020 · Genes & Development
The Mechanisms of Genome Editing Technologies in Crop Plants
Yumna Ahmad, Saqlain Haider, Javed Iqbal, Banzeer Ahsan Abbasi, Tabassum Yaseen, Tariq Mahmood · 2022 · Springer eBooks
Base editor screens for in situ mutational scanning at scale
Nicholas Z. Lue, Brian B. Liau · 2023 · Molecular Cell
Engineered IscB–ωRNA system with expanded target range for base editing
Qingquan Xiao, Guoling Li, Dingyi Han, Haoqiang Wang, Mingyu Yao, Tingting Ma, Jingxing Zhou, Yu Zhang, Xiumei Zhang, Bingbing He et al. · 2024 · Nature Chemical Biology
Advances in CRISPR/Cas9-Based Gene Editing in Filamentous Fungi
Bin Ma, Yimiao Li, Ting‐Hui Wang, Dongming Li, Shuang Jia · 2025 · Journal of Fungi
Multiplex precise base editing in cynomolgus monkeys
Wenhui Zhang, Tomomi Aida, Ricardo C.H. del Rosario, Jonathan J. Wilde, Chenhui Ding, Xiaohui Zhang, Zulqarnain Baloch, Yan Huang, Yu Tang, Duanduan Li et al. · 2020 · Nature Communications
Therapeutic applications of CRISPR/Cas9 in breast cancer and delivery potential of gold nanomaterials
Jananee Padayachee, Moganavelli Singh · 2020 · Nanobiomedicine
Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range
Ryoya Nakagawa, Soh Ishiguro, Sae Okazaki, Hideto Mori, Mamoru Tanaka, Hiroyuki Aburatani, Nozomu Yachie, Hiroshi Nishimasu, Osamu Nureki · 2022 · Communications Biology
The Bibliometric Landscape of Gene Editing Innovation and Regulation in the Worldwide
Xun Wei, Aqing Pu, Qianqian Liu, Quancan Hou, Yong Zhang, Xueli An, Yan Long, Yilin Jiang, Zhenying Dong, Suowei Wu et al. · 2022 · Cells
Utilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality
Shen, Qiqing, Ruan, Haihua, Zhang, Hongyang, Wu, Tao, Zhu, Kexin, Han, Wenying, Dong, Rui, Ming, Tianwei, Qi, Haikun, Zhang, Yan · 2024 · Frontiers in Microbiology
Research progress in gene editing technology
Sheng Huang, Yali Yan, Fei Su, Xiaorui Huang, Dandan Xia, Xinxin Jiang, Yinghui Dong, Ping Lv, Fangyuan Chen, Yanwei Lv · 2021 · Frontiers in Bioscience-Landmark
Recent advances and challenges in potato improvement using CRISPR/Cas genome editing
Izabela Chincinska, Magdalena Miklaszewska, Dorota Sołtys‐Kalina · 2022 · Planta
Shuttle peptide delivers base editor RNPs to rhesus monkey airway epithelial cells in vivo
Katarina Kulhánková, Soumba Traore, Xue Cheng, Hadrien Benk-Fortin, Stéphanie Hallée, Mario Harvey, Joannie Roberge, Frédéric Couture, Sajeev Kohli, Thomas J Gross et al. · 2023 · Nature Communications
Allele-specific genome targeting in the development of precision medicine
Junjiao Wu, Beisha Tang, Yu Tang · 2020 · Theranostics
A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing
Li Chun Yang, Jia Chen · 2020 · Trends in Biochemical Sciences
A short overview of CRISPR-Cas technology and its application in viral disease control
Abozar Ghorbani, Shima Hadifar, Roya Salari, K. Izadpanah, Michał Burmistrz, Alireza Afsharifar, Mohammad Hadi Eskandari, Ali Niazi‎, Christopher E. Denes, G. Gregory Neely · 2021 · Transgenic Research
Mutation-specific reporter for optimization and enrichment of prime editing
Imre Firmin Schene, Indi P. Joore, Jan H. L. Baijens, Remi Stevelink, Gautam Kok, Sawsan Shehata, Eveline F. Ilcken, E. C. M. Nieuwenhuis, Dian Bolhuis, R. C. M. van Rees et al. · 2022 · Nature Communications
Prime Editing: An All-Rounder for Genome Editing
Chenyu Lu, Jingyu Kuang, Tong Shao, Sisi Xie, Ming Li, Lingyun Zhu, Lvyun Zhu · 2022 · International Journal of Molecular Sciences
Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells
Wen Xu, Yongxing Yang, Ya Liu, Guiting Kang, Feipeng Wang, Lu Li, Xinxin Lv, Si Zhao, Shuang Yuan, Jinling Song et al. · 2019 · Molecular Plant
Precise CAG repeat contraction in a Huntington’s Disease mouse model is enabled by gene editing with SpCas9-NG
Seiya Oura, Taichi Noda, Naoko Morimura, Seiji Hitoshi, Hiroshi Nishimasu, Yoshitaka Nagai, Osamu Nureki, Masahito Ikawa · 2021 · Communications Biology
Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes
Rhiannon B. Werder, Joseph E. Kaserman, Michael S. Packer, Jonathan Lindstrom-Vautrin, Carlos Villacorta-Martín, Lauren E. Young, Yvonne Aratyn-Schaus, Francine M. Gregoire, Andrew A. Wilson · 2021 · Molecular Therapy
Strategies for High-Efficiency Mutation Using the CRISPR/Cas System
Shuying Feng, Zilong Wang, Aifang Li, Xin Xie, Junjie Liu, Shuxuan Li, Yalan Li, Baiyan Wang, Lina Hu, Lianhe Yang et al. · 2022 · Frontiers in Cell and Developmental Biology
Knockout of circRNAs by base editing back-splice sites of circularized exons
Xiang Gao, Xu-Kai Ma, Xiang Li, Guo‐Wei Li, Chu‐Xiao Liu, Jun Zhang, Ying Wang, Jia Wei, Jia Chen, Ling‐Ling Chen et al. · 2022 · Genome biology
Manipulating the 3D organization of the largest synthetic yeast chromosome
Weimin Zhang, Luciana Lazar‐Stefanita, Hitoyoshi Yamashita, Michael J. Shen, LESLIE ANN MITCHELL, Hikaru Kurasawa, Evgenii Lobzaev, Viola Fanfani, Max A. B. Haase, Xiaoji Sun et al. · 2023 · Molecular Cell
Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)
Minghui Guo, Kuan Ren, Yuwei Zhu, Z. Huang · 2018 · Worldwide Protein Data Bank
Targeted Knockout of the Vegfa Gene in the Retina by Subretinal Injection of RNP Complexes Containing Cas9 Protein and Modified SgRNAs
Holmgaard, Andreas Braae, Askou, Anne Louise, Jensen, Emilie Grarup, Alsing, Sidsel, Bak, Rasmus O., Mikkelsen, Jacob Giehm, Corydon, Thomas J. · 2021 · Molecular Therapy
Engineering APOBEC3A deaminase for highly accurate and efficient base editing
Lei Yang, Yanan Huo, Man Wang, Dan Zhang, Tianai Zhang, Hao Wu, Xichen Rao, Haowei Meng, Shuming Yin, Jiale Mei et al. · 2024 · Nature Chemical Biology
Assessing and engineering the IscB–ωRNA system for programmed genome editing
Hao Yan, Xiaoqing Tan, Siyuan Zou, Yihong Sun, Ailong Ke, Weixin Tang · 2024 · Nature Chemical Biology
Restore natural fertility of Kitw/Kitwv mouse with nonobstructive azoospermia through gene editing on SSCs mediated by CRISPR-Cas9
Xiaoyu Li, Tie‐Cheng Sun, Xiuxia Wang, Ji‐Xin Tang, Yixun Liu · 2019 · Stem Cell Research & Therapy
CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)
Yoshihiko Nanasato, Masafumi Mikami, Norihiro Futamura, M. Endo, Mitsuru Nishiguchi, Yasunori Ohmiya, Ken-ichi Konagaya, Toru Taniguchi · 2021 · Scientific Reports
Improved base editor for efficient editing in GC contexts in rabbits with an optimized AID‐Cas9 fusion
Zhiquan Liu, Huanhuan Shan, Siyu Chen, Mao Chen, Quanjun Zhang, Liangxue Lai, Zhanjun Li · 2019 · The FASEB Journal
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness
Chong Cui, Shengyi Wang, Daqi Wang, Jingjing Zhao, Bowei Huang, Biyun Zhu, Yuxin Chen, Honghai Tang, Yu Han, Ye Cheng et al. · 2024 · Nature Biomedical Engineering
The ScCas9++ variant expands the CRISPR toolbox for genome editing in plants
Taoli Liu, Dongchang Zeng, Zhiye Zheng, Zhansheng Lin, Yang Xue, Tie Li, Xianrong Xie, Genglu Ma, Yao‐Guang Liu, Qinlong Zhu · 2021 · Journal of Integrative Plant Biology
A large-scale genome and transcriptome sequencing analysis reveals the mutation landscapes induced by high-activity adenine base editors in plants
Shaofang Li, Lang Liu, Wenxian Sun, Xueping Zhou, Huanbin Zhou · 2022 · Genome biology
Experimental demonstration of tethered gene drive systems for confined population modification or suppression
Matthew Metzloff, Emily Yang, Sumit Dhole, Andrew G. Clark, Philipp W. Messer, Jackson Champer · 2022 · BMC Biology
Closely related type II-C Cas9 orthologs recognize diverse PAMs
Jingjing Wei, Linghui Hou, Jingtong Liu, Ziwen Wang, Siqi Gao, Tao Qi, Song Gao, Shuna Sun, Yongming Wang · 2022 · eLife
DNA topology regulates PAM-Cas9 interaction and DNA unwinding to enable near-PAMless cleavage by thermophilic Cas9
Ya-Jing Shi, Min Duan, Junmei Ding, Fan-Qi Wang, Li-Li Bi, Cai-Xiang Zhang, Yizhou Zhang, Jun-Yi Duan, An-Hui Huang, Xinlin Lei et al. · 2022 · Molecular Cell
CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering
Katja Glinšek, Krištof Bozovičar, Tomaž Bratkovič · 2023 · International Journal of Molecular Sciences
A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid
Tiantian Chang, Weichao Ding, Shirui Yan, Yun Wang, Haoling Zhang, Yu Zhang, Zhi Ping, Huiming Zhang, Yijian Huang, Jiahui Zhang et al. · 2023 · Nature Communications
Base editing of trinucleotide repeats that cause Huntington’s disease and Friedreich’s ataxia reduces somatic repeat expansions in patient cells and in mice
Żaneta Matuszek, Mandana Arbab, Maheswaran Kesavan, Alvin Hsu, Jennie C. L. Roy, Jing Zhao, Tian Yu, Ben Weisburd, Gregory A. Newby, Neil J. Doherty et al. · 2025 · Nature Genetics
Single crossover-mediated targeted nucleotide substitution and knock-in strategies with CRISPR/Cas9 system in the rice blast fungus
Tohru Yamato, Ai Handa, Takayuki Arazoe, Misa Kuroki, Akihito Nozaka, Takashi Kamakura, Shuichi Ohsato, Tsutomu Arie, Shigeru Kuwata · 2019 · Scientific Reports
Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells
Lianchao Tang, Fayu Yang, Xiaoxue He, Haihua Xie, Xiaoyu Liu, Junhao Fu, Haitao Xi, Xiaosheng Lu, Changbao Liu, Zongming Song et al. · 2019 · Cell Regeneration
Rational engineering of a modular bacterial CRISPR–Cas activation platform with expanded target range
Maria Claudia Villegas Kcam, Annette J. Tsong, James Chappell · 2021 · Nucleic Acids Research
Using CRISPR to understand and manipulate gene regulation
Ersin Akıncı, Marisa C. Hamilton, Benyapa Khowpinitchai, Richard I. Sherwood · 2021 · Development
Engineering Cas9 for human genome editing
Ian M. Slaymaker, Nicole M. Gaudelli · 2021 · Current Opinion in Structural Biology
Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
Jian Wang, Yuxi Teng, Ruihua Zhang, Yifei Wu, Lei Lou, Yusong Zou, Michelle Li, Zhong‐Ru Xie, Yajun Yan · 2021 · Nature Communications
Revisiting CRISPR/Cas-mediated crop improvement: special focus on nutrition
Kaul, Tanushri, Sony, Sonia Khan, Verma, Rachana, Motelb, Khaled Fathy Abdel, Prakash, Arul T, Eswaran, Murugesh, Bharti, Jyotsna, Nehra, Mamta, Kaul, Rashmi · 2020 · Journal of Biosciences
Expanding base editing scope to near-PAMless with engineered CRISPR/Cas9 variants in plants
Chengwei Zhang, Yao Wang, Feipeng Wang, Si Zhao, Jinling Song, Feng Feng, Jiuran Zhao, Jinxiao Yang · 2020 · Molecular Plant
The efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana
Min‐Kyeung Choi, Jae‐Young Yun, Jun‐Hyuk Kim, Sang‐Tae Kim, Sang‐Tae Kim · 2021 · Scientific Reports
A humanized knockin mouse model of Duchenne muscular dystrophy and its correction by CRISPR-Cas9 therapeutic gene editing
Yu Zhang, Hui zi Li, Takahiko Nishiyama, John McAnally, Efrain Sanchez‐Ortiz, Jian Hua Huang, Pradeep P.A. Mammen, Rhonda Bassel‐Duby, Eric N. Olson · 2022 · Molecular Therapy — Nucleic Acids
Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli
Rahul Mahadev Shelake, Dibyajyoti Pramanik, Jae‐Yean Kim · 2023 · mBio
Base editors: development and applications in biomedicine
Yanhui Liang, Fangbing Chen, Kepin Wang, Liangxue Lai · 2023 · Frontiers of Medicine
CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications
Diego Alba Burbano, Cholpisit Kiattisewee, Ava V. Karanjia, Ryan A. L. Cardiff, Ian Faulkner, Widianti Sugianto, James M. Carothers · 2024 · Annual Review of Chemical and Biomolecular Engineering
CRISPR/Cas-Mediated Genome Engineering in Plants: Application and Prospectives
Swetaleena Mishra, Subhendu Nayak, Narendra Tuteja, Sowmya Poosapati, Durga Madhab Swain, Ranjan Kumar Sahoo · 2024 · Plants
Harnessing the evolving CRISPR/Cas9 for precision oncology
Tianye Li, Shuiquan Li, Yue Kang, Jianwei Zhou, Ming Yi · 2024 · Journal of Translational Medicine
Competitive dCas9 binding as a mechanism for transcriptional control
Daniel A. Anderson, Christopher A. Voigt · 2021 · Molecular Systems Biology
Allele-specific silencing of the gain-of-function mutation in Huntington’s disease using CRISPR/Cas9
Jun Wan Shin, Eun Pyo Hong, Seri S. Park, Doo Eun Choi, Ihn Sik Seong, Madelynn N. Whittaker, Benjamin P. Kleinstiver, Richard Z. Chen, Jong‐Min Lee · 2022 · JCI Insight
CRISPR-Based Therapeutic Gene Editing for Duchenne Muscular Dystrophy: Advances, Challenges and Perspectives
Guofang Chen, Tingyi Wei, Hui Yang, Guoling Li, Haisen Li · 2022 · Cells
Reversing the Central Dogma: RNA-guided control of DNA in epigenetics and genome editing
Howard Y. Chang, Lei S. Qi · 2023 · Molecular Cell
Improving recombinant protein production in CHO cells using the CRISPR-Cas system
Ali Kerem Kalkan, Fahreddin Palaz, Sofija Semeniuk, Nada Elmousa, Yuri Ledezma, Élise Cachat, Leonardo Rios‐Solis · 2023 · Biotechnology Advances
Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors
Ao Zhang, Tiaofeng Shan, Yan Sun, Zhipeng Chen, Jianjian Hu, Zhichao Hu, Ziheng Ming, Zhitao Zhu, Xue Li, Jun He et al. · 2023 · Plant Biotechnology Journal
Programmable adenine deamination in bacteria using a Cas9–adenine-deaminase fusion
Ya Ping Zhang, Hongyuan Zhang, Zhipeng Wang, Zhaowei Wu, Yu Wang, Na Tang, Xuexia Xu, Suwen Zhao, Weizhong Chen, Quanjiang Ji · 2020 · Chemical Science
PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells
Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono et al. · 2023 · Communications Medicine
Developing ABEmax-NG with Precise Targeting and Expanded Editing Scope to Model Pathogenic Splice Site Mutations In Vivo
Shisheng Huang, Zhaodi Liao, Xiangyang Li, Zhen Liu, Guanglei Li, Jianan Li, Zongyang Lu, Yu Zhang, Xiajun Li, Xu Ma et al. · 2019 · iScience
CRISPR-Cas nucleases and base editors for plant genome editing
Filiz Gürel, Yingxiao Zhang, Simon Sretenovic, Yiping Qi · 2019 · aBIOTECH
Single-swap editing for the correction of common Duchenne muscular dystrophy mutations
Andreas C. Chai, Francesco Chemello, Hui zi Li, Takahiko Nishiyama, Kenian Chen, Yu Zhang, Efrain Sanchez‐Ortiz, Adeeb Alomar, Lin Xu, Ning Liu et al. · 2023 · Molecular Therapy — Nucleic Acids
Next-generation forward genetic screens: uniting high-throughput perturbations with single-cell analysis
John A. Morris, Jennifer S. Sun, Neville E. Sanjana · 2023 · Trends in Genetics
Breaking genetic shackles: The advance of base editing in genetic disorder treatment
Fang Xu, Caiyan Zheng, Weihui Xu, Shiyao Zhang, Shanshan Liu, Xiaopeng Chen, Kai Yao · 2024 · Frontiers in Pharmacology
Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing
Honglue Shi, Noor Al-Sayyad, Kevin M. Wasko, Marena I. Trinidad, Erin Doherty, Kamakshi Vohra, Ron S. Boger, David Colognori, Joshua C. Cofsky, Petr Skopintsev et al. · 2025 · Molecular Cell
LIM and SH3 protein 1 induces glioma growth and invasion through PI3K/AKT signaling and epithelial-mesenchymal transition
Chuanhong Zhong, Xianglong Li, Bei Tao, Lilei Peng, Tangming Peng, Xiaobo Yang, Xiangguo Xia, Ligang Chen · 2019 · Biomedicine & Pharmacotherapy
Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice
Jin-Jing Li, Xiang Lin, Cheng Tang, Ying‐Qian Lu, Xinde Hu, Erwei Zuo, He Li, Wenqin Ying, Yidi Sun, Lu‐Lu Lai et al. · 2019 · National Science Review
CRISPR/Cas System: A Potential Technology for the Prevention and Control of COVID-19 and Emerging Infectious Diseases
Ronghua Ding, Jinzhao Long, Mingzhu Yuan, Yuefei Jin, Haiyan Yang, Mengshi Chen, Shuaiyin Chen, Guangcai Duan · 2021 · Frontiers in Cellular and Infection Microbiology
Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease
Weizhong Chen, Hongyuan Zhang, Yifei Zhang, Yu Wang, Jianhua Gan, Quanjiang Ji · 2019 · PLoS Biology
Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington’s disease
Doo Eun Choi, Jun Wan Shin, Sophia Zeng, Eun Pyo Hong, Jae-Hyun Jang, Jacob M. Loupe, Vanessa C. Wheeler, Hannah E. Stutzman, Ben Kleinstiver, Jong‐Min Lee · 2023 · eLife
Expanding plant genome editing scope and profiles with CRISPR‐FrCas9 systems targeting palindromic TA sites
Yao He, Yangshuo Han, Yanqin Ma, Shishi Liu, Tingting Fan, Yanling Liang, Xu Tang, Xuelian Zheng, Yuechao Wu, Tao Zhang et al. · 2024 · Plant Biotechnology Journal
ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish
Wei Qin, Fang Liang, Sheng‐Jia Lin, Cassidy Petree, Kevin Huang, Yu Zhang, Lin Li, Pratishtha Varshney, Philippe Mourrain, Yanmei Liu et al. · 2024 · Nature Communications
CRISPR Diagnosis and Therapeutics with Single Base Pair Precision
Seung Hwan Lee, Young‐Ho Park, Yeung Bae Jin, Sun‐Uk Kim, Junho K. Hur · 2019 · Trends in Molecular Medicine
A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis
Shuta Kurokawa, Hafizur Rahman, Naoshi Yamanaka, Chisato Ishizaki, Shaikhul Islam, Tsuyoshi Aiso, Shunya Hirata, Mayuka Yamamoto, Kappei Kobayashi, Hidetaka Kaya · 2021 · Plant and Cell Physiology
Expansion of the prime editing modality with Cas9 from Francisella novicida
Yeounsun Oh, Wi-jae Lee, Junho K. Hur, Woojeung Song, Youngjeon Lee, Hanseop Kim, Lee Wha Gwon, Younghyun Kim, Young‐Ho Park, Chan Hyoung Kim et al. · 2022 · Genome biology