Engineered CRISPR-Cas9 nuclease with expanded targeting space

Coot : model-building tools for molecular graphics

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XDS

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Multiplex Genome Engineering Using CRISPR/Cas Systems

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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

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DNA targeting specificity of RNA-guided Cas9 nucleases

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How good are my data and what is the resolution?

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Molecular replacement with MOLREP

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A base editing strategy using mRNA-LNPs for in vivo correction of the most frequent phenylketonuria variant

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In vivo continuous evolution of metabolic pathways for chemical production

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Transient cotransformation of CRISPR /Cas9 and oligonucleotide templates enables efficient editing of target loci in Physcomitrella patens

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CrisPam: SNP-Derived PAM Analysis Tool for Allele-Specific Targeting of Genetic Variants Using CRISPR-Cas Systems

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CRISPR-dependent base editing as a therapeutic strategy for rare monogenic disorders

Cabré-Romans, J.-J., & Cuella-Martin, R. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1553590 · Cited by 33

CRISPR-based therapeutic genome editing for inherited blood disorders

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Genome editing using CRISPR/Cas9 to treat hereditary hematological disorders

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Versatile and efficient in vivo genome editing with compact Streptococcus pasteurianus Cas9

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Development of Lipid Nanoparticles for the Delivery of Macromolecules Based on the Molecular Design of pH-Sensitive Cationic Lipids

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Cas12a-Based Diagnostics for Potato Purple Top Disease Complex Associated with Infection by ‘ Candidatus Phytoplasma trifolii’-Related Strains

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Bibliometric Analysis of Functional Crops and Nutritional Quality: Identification of Gene Resources to Improve Crop Nutritional Quality through Gene Editing Technology

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Generation of induced pluripotent stem cells-derived hepatocyte-like cells for ex vivo gene therapy of primary hyperoxaluria type 1

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Quantification of the affinities of CRISPR–Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences

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Genetic control of invasive sea lamprey in the Great Lakes

Ferreira-Martins, D., Champer, J., McCauley, D. W., Zhang, Z., & Docker, M. F. (2021) · Journal of Great Lakes Research · doi:10.1016/j.jglr.2021.10.018 · Cited by 28

Prime Editing: A Revolutionary Technology for Precise Treatment of Genetic Disorders

Li, M., Lin, Y., Cheng, Q., & Wei, T. (2025) · Cell Proliferation · doi:10.1111/cpr.13808 · Cited by 28

Production of Herbicide-Sensitive Strain to Prevent Volunteer Rice Infestation Using a CRISPR-Cas9 Cytidine Deaminase Fusion

Komatsu, A., Ohtake, M., Shimatani, Z., & Nishida, K. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00925 · Cited by 27

Base editing in rice: current progress, advances, limitations, and future perspectives

Yarra, R., & Sahoo, L. (2021) · Plant Cell Reports · doi:10.1007/s00299-020-02656-3 · Cited by 29

Role of macrophage polarization in osteoarthritis (Review)

Chen, B., Hong, H., Sun, Y., Chen, C., Wu, C., Xu, G., Bao, G., & Cui, Z. (2022) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2022.11693 · Cited by 27

Recent advances in therapeutic CRISPR-Cas9 genome editing: mechanisms and applications

Zhou, L., & Yao, S. (2023) · Molecular Biomedicine · doi:10.1186/s43556-023-00115-5 · Cited by 29

Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses

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Automated design of CRISPR prime editors for 56,000 human pathogenic variants

Morris, J. A., Rahman, J. A., Guo, X., & Sanjana, N. E. (2021) · iScience · doi:10.1016/j.isci.2021.103380 · Cited by 28

Machine learning-based prediction models to guide the selection of Cas9 variants for efficient gene editing

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Development of multiplexed orthogonal base editor (MOBE) systems

Cowan, Q. T., Gu, S., Gu, W., Ranzau, B. L., Simonson, T. S., & Komor, A. C. (2025) · Nature Biotechnology · doi:10.1038/s41587-024-02240-0 · Cited by 26

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

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Exploring the potential of cell-derived vesicles for transient delivery of gene editing payloads

Leandro, K., Rufino‐Ramos, D., Breyne, K., Ianni, E. D., Lopes, S., Nobre, R. J., Kleinstiver, B. P., Perdigão, P. R. L., Breakefield, X. O., & de Almeida, L. P. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115346 · Cited by 27

The CRISPR-Cas system in molecular diagnostics

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Gene editing: an instrument for practical application of gene biology to plant breeding

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Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs

Wang, Y., Bi, D., Qin, G., Song, R., Yao, J. T., Cao, C., Zheng, Q., Hou, N., Wang, Y., & Zhao, J. (2020) · Frontiers in Genetics · doi:10.3389/fgene.2020.592623 · Cited by 28

Basic Principles and Clinical Applications of CRISPR-Based Genome Editing

Lim, J. M., & Kim, S. (2022) · Yonsei Medical Journal · doi:10.3349/ymj.2022.63.2.105 · Cited by 26

Engineering Cas9: next generation of genomic editors

Kovalev, M. A., Davletshin, A. I., & Karpov, D. S. (2024) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-024-13056-y · Cited by 29

Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

Zhu, Y., & Huang, Z. (2018) · National Science Review · doi:10.1093/nsr/nwy150 · Cited by 26

Plant genome editing using xCas9 with expanded PAM compatibility

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Engineered dual selection for directed evolution of SpCas9 PAM specificity

Goldberg, G. W., Spencer, J. M., Giganti, D. O., Camellato, B. R., Agmon, N., Ichikawa, D. M., Boeke, J. D., & Noyes, M. B. (2021) · Nature Communications · doi:10.1038/s41467-020-20650-x · Cited by 29

CRISPR-Cas9 and beyond: what’s next in plant genome engineering

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Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells

Escobar, M., Li, J., Patel, A., Liu, S., Xu, Q., & Hilton, I. B. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00156 · Cited by 25

Future Perspectives of Prime Editing for the Treatment of Inherited Retinal Diseases

Hansen, S., McClements, M. E., Corydon, T. J., & MacLaren, R. E. (2023) · Cells · doi:10.3390/cells12030440 · Cited by 25

Advances in therapeutic application of CRISPR-Cas9

Sun, J., Wang, J., Zheng, D., & Hu, X. (2019) · Briefings in Functional Genomics · doi:10.1093/bfgp/elz031 · Cited by 25

Efficient Genome Editing in Bacillus licheniformis Mediated by a Conditional CRISPR/Cas9 System

Li, Y., Wang, H., Zhang, L. N., Ding, Z., Xu, S., Gu, Z., & Shi, G. (2020) · Microorganisms · doi:10.3390/microorganisms8050754 · Cited by 25

Emerging Gene-Editing Modalities for Osteoarthritis

Tanikella, A. S., Hardy, M. J., Frahs, S. M., Cormier, A. G., Gibbons, K. D., Fitzpatrick, C. K., & Oxford, J. T. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176046 · Cited by 25

Correction of human nonsense mutation via adenine base editing for Duchenne muscular dystrophy treatment in mouse

Jin, M., Lin, J., Li, H., Li, Z., Dong, Y., Wang, Y., Yu, Y., Shao, Z., Chen, L., Wang, Z., et al. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102165 · Cited by 27

Functional Genomics in Pancreatic β Cells: Recent Advances in Gene Deletion and Genome Editing Technologies for Diabetes Research

Hu, M., Cherkaoui, I., Misra, S., & Rutter, G. A. (2020) · Frontiers in Endocrinology · doi:10.3389/fendo.2020.576632 · Cited by 24

CRISPR-based genome editing technology and its applications in oil crops

He, J., Zhang, K., Tang, M., Zhou, W., Chen, L., Chen, Z., & Li, M. (2021) · Oil Crop Science · doi:10.1016/j.ocsci.2021.07.002 · Cited by 24

Gene-by-environment interactions are pervasive among natural genetic variants

Chen, S.-A. A., Kern, A., Ang, R. M. L., Xie, Y., & Fraser, H. B. (2023) · Cell Genomics · doi:10.1016/j.xgen.2023.100273 · Cited by 25

Establishing chromosomal design-build-test-learn through a synthetic chromosome and its combinatorial reconfiguration

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Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNAGln-processing system in human cells

He, X., Wang, Y., Yang, F., Wang, B., Xie, H., Gu, L., Zhao, T., Liu, X., Zhang, D., Ren, Q., et al. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.007791 · Cited by 24

Generation of imidazolinone herbicide resistant trait in Arabidopsis

Dong, H., Wang, D., Bai, Z., Yuan, Y., Yang, W., Zhang, Y., Ni, H., & Jiang, L. (2020) · PLoS ONE · doi:10.1371/journal.pone.0233503 · Cited by 24

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

Collias, D., Leenay, R. T., Slotkowski, R., Zuo, Z., Collins, S. P., McGirr, B. A., Liu, J., & Beisel, C. L. (2020) · Science Advances · doi:10.1126/sciadv.abb4054 · Cited by 24

Gene therapy in cardiac and vascular diseases: a review of approaches to treat genetic and common cardiovascular diseases with novel gene-based therapeutics

Musolino, P. L., Rosser, S. J., Brittan, M., Newby, D. E., Berry, C., Riley, P. R., Giacca, M., Hajjar, R. J., & Baker, A. H. (2025) · Cardiovascular Research · doi:10.1093/cvr/cvaf109 · Cited by 23

Research progress of base editing and prime editing tools based on the CRISPR/Cas system

Gao, H., Gao, S., Kan, G., Valentovich, L. N., & An, Y. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102771 · Cited by 26

Compact SchCas9 Recognizes the Simple NNGR PAM

Wang, S., Mao, H., Hou, L., Hu, Z., Wang, Y., Qi, T., Tao, C., Yang, Y., Zhang, C., Li, M., et al. (2021) · Advanced Science · doi:10.1002/advs.202104789 · Cited by 24

Increasing the Targeting Scope of CRISPR Base Editing System Beyond NGG

Yu, S.-Y., Birkenshaw, A., Thomson, T., Carlaw, T., Zhang, L., & Ross, C. J. (2022) · The CRISPR Journal · doi:10.1089/crispr.2021.0109 · Cited by 23

In Vivo Hematopoietic Stem Cell Genome Editing: Perspectives and Limitations

Psatha, N., Paschoudi, K., Παπαδοπούλου, Α., & Yannaki, E. (2022) · Genes · doi:10.3390/genes13122222 · Cited by 24

Bioadhesive and Injectable Hydrogels and Their Correlation with Mesenchymal Stem Cells Differentiation for Cartilage Repair: A Mini-Review

Kováč, J., Priščáková, P., Gbelcová, H., Heydari, A., & Žiaran, S. (2023) · Polymers · doi:10.3390/polym15214228 · Cited by 23

SpRY: Engineered CRISPR/Cas9 Harnesses New Genome-Editing Power

Zhang, D., & Zhang, B. (2020) · Trends in Genetics · doi:10.1016/j.tig.2020.05.001 · Cited by 23

Domain-inlaid Nme2Cas9 adenine base editors with improved activity and targeting scope

Bamidele, N., Zhang, H., Dong, X., Cheng, H., Gaston, N., Feinzig, H., Cao, H., Kelly, K. L., Watts, J. K., Xie, J., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-45763-5 · Cited by 24

CRISPR/Cas system-mediated base editing in crops: recent developments and future prospects

Hillary, V. E., & Ceasar, S. A. (2024) · Plant Cell Reports · doi:10.1007/s00299-024-03346-0 · Cited by 24

Recent advances in CRISPR-Cas system for Saccharomyces cerevisiae engineering

Wu, X., Wan, X., Yu, H., & Liu, H. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108557 · Cited by 23

The evolving CRISPR technology

Yan, M., & Li, J. (2019) · Protein & Cell · doi:10.1007/s13238-019-0645-9 · Cited by 22

CRISPR applications for Duchenne muscular dystrophy: From animal models to potential therapies

Chey, Y. C. J., Arudkumar, J., Aartsma-Rus, A. M., Adikusuma, F., & Thomas, P. Q. (2022) · WIREs Mechanisms of Disease · doi:10.1002/wsbm.1580 · Cited by 24

Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells

Li, C., Liu, Z., Anderson, J. D., Liu, Z., Tang, L., Yao, L., Peng, N., Chen, J., Liu, X., Fu, L., et al. (2023) · PLoS ONE · doi:10.1371/journal.pone.0295009 · Cited by 21

Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM

Qi, T., Wang, Y., Yang, Y., Gao, S., Liu, J., Huang, Q., Tian, Y., Tang, J., Zheng, W., & Wang, Y. (2023) · Nature Chemical Biology · doi:10.1038/s41589-023-01481-5 · Cited by 23

Potential therapeutic strategies for osteoarthritis via CRISPR/Cas9 mediated gene editing

Vlashi, R., Zhang, X., Li, H., & Chen, G. (2023) · Reviews in Endocrine and Metabolic Disorders · doi:10.1007/s11154-023-09860-y · Cited by 24

Label-Free Nuclease Assay with Long-Term Stability

Liu, R., Hu, J., Chen, Y., Jiang, M., & Lv, Y. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b02467 · Cited by 22

CRISPR-Cas “Non-Target” Sites Inhibit On-Target Cutting Rates

Moreb, E. A., Hutmacher, M., & Lynch, M. (2020) · The CRISPR Journal · doi:10.1089/crispr.2020.0065 · Cited by 23

Progress and challenges in CRISPR-mediated therapeutic genome editing for monogenic diseases

Konishi, C. T., & Long, C. (2021) · Journal of Biomedical Research · doi:10.7555/jbr.34.20200105 · Cited by 26

How to use CRISPR/Cas9 in plants: from target site selection to DNA repair

Přibylová, A., & Fischer, L. (2024) · Journal of Experimental Botany · doi:10.1093/jxb/erae147 · Cited by 24

Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology

Menon, A. V., Song, B., Chao, L., Sriram, D., Chansky, P. A., Bakshi, I., Ulianova, J., & Li, W. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1565387 · Cited by 21

Knock-in and precise nucleotide substitution using near-PAMless engineered Cas9 variants in Dictyostelium discoideum

Asano, Y., Yamashita, K., Hasegawa, A., Ogasawara, T., Iriki, H., & Muramoto, T. (2021) · Scientific Reports · doi:10.1038/s41598-021-89546-0 · Cited by 24

Gradients in gene essentiality reshape antibacterial research

Hogan, A. M., & Cardona, S. T. (2022) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuac005 · Cited by 24

Improvements of nuclease and nickase gene modification techniques for the treatment of genetic diseases

Lu, Y., Mbakam, C. H., Song, B., Bendavid, E., & Tremblay, J. P. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.892769 · Cited by 22

Multiplex base editing to convert TAG into TAA codons in the human genome

Chen, Y., Hysolli, E., Chen, A., Casper, S., Liu, S., Yang, Y., Liu, C., & Church, G. M. (2022) · Nature Communications · doi:10.1038/s41467-022-31927-8 · Cited by 22

Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants

Kiattisewee, C., Karanjia, A. V., Legut, M., Daniloski, Z., Koplik, S. E., Nelson, J., Kleinstiver, B. P., Sanjana, N. E., Carothers, J. M., & Zalatan, J. G. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00405 · Cited by 21

Multiple waves of fetal‐derived immune cells constitute adult immune system

Kobayashi, M., & Yoshimoto, M. (2023) · Immunological Reviews · doi:10.1111/imr.13192 · Cited by 21

Advances in CRISPR/Cas gene therapy for inborn errors of immunity

Liu, X., Li, G., Liu, Y., Zhou, F., Huang, X., & Li, K. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1111777 · Cited by 21

Massively parallel evaluation and computational prediction of the activities and specificities of 17 small Cas9s

Seo, S.-Y., Min, S., Lee, S., Seo, J. H., Park, J., Kim, H. K., Song, M., Baek, D., Cho, S., & Kim, S. (2023) · Nature Methods · doi:10.1038/s41592-023-01875-2 · Cited by 21

Efficient base editing with expanded targeting scope using an engineered Spy-mac Cas9 variant

Liu, Z., Shan, H., Chen, S., Chen, M., Song, Y., Lai, L., & Li, Z. (2019) · Cell Discovery · doi:10.1038/s41421-019-0128-4 · Cited by 21

Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope

Villiger, L., Schmidheini, L., Mathis, N., Rothgangl, T., Marquart, K. F., & Schwank, G. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.08.025 · Cited by 21

Hybrid Cas12a Variants with Relaxed PAM Requirements Expand Genome Editing Compatibility

Liu, Z., Liu, H., Huang, C., Zhou, Q., & Luo, Y. (2024) · ACS Synthetic Biology · doi:10.1021/acssynbio.4c00103 · Cited by 20

CRISPR Technology and Its Emerging Applications

Zhang, X. E., Ma, D., & Liu, F. (2025) · Genomics Proteomics & Bioinformatics · doi:10.1093/gpbjnl/qzaf034 · Cited by 21

High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase

Wu, Y., Xiao, Y., & Tang, W. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02678-w · Cited by 23

CRISPR screening strategies for microRNA target identification

Yang, B., & McJunkin, K. (2020) · FEBS Journal · doi:10.1111/febs.15218 · Cited by 20

Sophisticated CRISPR/Cas tools for fine-tuning plant performance

Capdeville, N., Merker, L., Schindele, P., & Puchta, H. (2020) · Journal of Plant Physiology · doi:10.1016/j.jplph.2020.153332 · Cited by 21

Improving the Microbial Production of Amino Acids: From Conventional Approaches to Recent Trends

Zhang, G., Ren, X., Liang, X., Wang, Y., Feng, D., Zhang, Y., Xian, M., & Zou, H. (2021) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-020-0390-1 · Cited by 20

New Hope for Genome Editing in Cultivated Grasses: CRISPR Variants and Application

Riaz, A., Kanwal, F., Ahmad, I., Ahmad, S., Farooq, A., Madsen, C. K., Brinch‐Pedersen, H., Bekalu, Z. E., Dai, F., Zhang, G., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.866121 · Cited by 20

Current therapies for osteoarthritis and prospects of CRISPR-based genome, epigenome, and RNA editing in osteoarthritis treatment

Chen, Y., Luo, X. L., Kang, R., Cui, K., Ou, J., Zhang, X., & Liang, P. (2023) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2023.07.007 · Cited by 23

Genetic Databases and Gene Editing Tools for Enhancing Crop Resistance against Abiotic Stress

Joshi, A. B., Yang, S.-Y., Song, H.-G., Min, J., & Lee, J.-H. (2023) · Biology · doi:10.3390/biology12111400 · Cited by 21

CrnnCrispr: An Interpretable Deep Learning Method for CRISPR/Cas9 sgRNA On-Target Activity Prediction

Zhu, W., Xie, H., Chen, Y., & Zhang, G. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25084429 · Cited by 22

Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces

Wang, J., Wang, K., Deng, Z., Zhong, Z., Sun, G., Mei, Q., Zhou, F., Deng, Z., & Sun, Y. (2024) · Nature Communications · doi:10.1038/s41467-024-49987-3 · Cited by 20

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Clarissa, E. M., Karmacharya, M., Choi, H., Kumar, S., & Cho, Y. (2025) · Small · doi:10.1002/smll.202409353 · Cited by 23

Modeling human point mutation diseases in Xenopus tropicalis with a modified CRISPR/Cas9 system

Shi, Z., Xin, H., Tian, D., Lian, J., Wang, J., Liu, G., Ran, R., Shi, S., Zhang, Z., Shi, Y., et al. (2019) · The FASEB Journal · doi:10.1096/fj.201802661r · Cited by 22

Expanding the base editing scope to GA and relaxed NG PAM sites by improved xC as9 system

Zhang, C., Xu, W., Wang, F., Kang, G., Yuan, S., Lv, X., Li, L., Liu, Y., & Yang, J. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13259 · Cited by 20

GTR 2.0: gRNA-tRNA Array and Cas9-NG Based Genome Disruption and Single-Nucleotide Conversion in Saccharomyces cerevisiae

Gong, G., Zhang, Y., Wang, Z., Liu, L., Shi, S., Siewers, V., Yuan, Q., Nielsen, J., Zhang, X., & Liu, Z. (2021) · ACS Synthetic Biology · doi:10.1021/acssynbio.0c00560 · Cited by 22

Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency

Bermudez-Cabrera, H. C., Culbertson, S. J., Barkal, S. A., Holmes, B., Shen, M. W., Zhang, S., Gifford, D. K., & Sherwood, R. I. (2021) · Nature Communications · doi:10.1038/s41467-021-25415-8 · Cited by 21

Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System

Ipoutcha, T., Rideau, F., Gourgues, G., Arfi, Y., Lartigue, C., Blanchard, A., & Sirand-Pugnet, P. (2022) · Applied and Environmental Microbiology · doi:10.1128/aem.00996-22 · Cited by 19

Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications

Clauer, P. R., Nou, A., Toth, T., Yu, Q., Chemla, Y., Boo, A., Yoon, K. S., & Voigt, C. A. (2026) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00687 · Cited by 21

Customizing CRISPR–Cas PAM specificity with protein language models

Nayfach, S., Bhatnagar, A., Novichkov, A., Kim, N., Hoffnagle, A. M., Hussain, R., Estevam, G. O., Hill, E., Ruffolo, J. A., Silverstein, R. A., et al. (2026) · Nature Biotechnology · doi:10.1038/s41587-025-02995-0 · Cited by 19

Therapeutic gene editing strategies using CRISPR-Cas9 for the β-hemoglobinopathies

Papizan, J. B., Porter, S. N., Sharma, A., & Pruett‐Miller, S. M. (2020) · Journal of Biomedical Research · doi:10.7555/jbr.34.20200096 · Cited by 21

CRISPR Toolbox for Genome Editing in Dictyostelium

Yamashita, K., Iriki, H., Kamimura, Y., & Muramoto, T. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.721630 · Cited by 23

Mechanistic insights into the versatile class II CRISPR toolbox

Zhang, F., & Huang, Z. (2021) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2021.11.007 · Cited by 20

Dissecting Plant Gene Functions Using CRISPR Toolsets for Crop Improvement

Zhang, R., Li, B., Yang, Z.-G., Huang, J., Sun, W.-H., Bhanbhro, N., Liu, W., & Chen, K. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c01754 · Cited by 20

Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM

Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 186

Expanding the targeting space of Cas9

Mao, S. (2018) · Science · doi:10.1126/science.361.6408.1211-g

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science · doi:10.1126/science.aba8853 · Cited by 1,233

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71626-2 · Cited by 2

Functional Characterization and Improved Engineering of the PAM-flexible Cas9 Variant, SpG-Cas9

Limbasia, S. (2023) · Texas Digital Library (University of Texas) · doi:10.26153/tsw/60656