Engineered CRISPR-Cas9 nuclease with expanded targeting space
Nishimasu, Hiroshi, Shi, Xi, Ishiguro, Soh, Gao, Linyi, Hirano, Seiichi, Okazaki, Sae, Noda, Taichi, Abudayyeh, Omar O., Gootenberg, Jonathan S., Mori, Hideto, Oura, Seiya, Holmes, Benjamin, Tanaka, Mamoru, Seki, Motoaki, Hirano, Hisato, Aburatani, Hiroyuki, Ishitani, Ryuichiro, Ikawa, Masahito, Yachie, Nozomu, Zhang, Feng, Nureki, Osamu·2018·Science
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- Nucleic acid-based therapeutic strategies for modulator-refractory cystic fibrosis-causing variantsMark D Leahy, Immacolata Zollo, Luka A. Clarke, Carlos Miguel Farinha · 2026 · Human Molecular Genetics
- Editing around the target: epitope engineering to protect stem cell graftsJoanne Seoyoung Baek, Gabriele Casirati, Pietro Genovese, Saar I. Gill · 2026 · Blood Advances
- Precision genetic manipulation: CRISPR toolkits reshape plant biologyZhenxiang Li, Xiangyu Xiong, Renqi Cao, Jianfeng Li · 2026 · PLANT PHYSIOLOGY
- Translating base editors: a decade of progress from the bench to the clinicNicole M. Gaudelli, Alexis C. Komor · 2026 · Nature Reviews Drug Discovery
- Efficient multinucleotide deletions using deaminase-Cas9 fusions in human cellsSiyu Chen, Zhiquan Liu, Hao Yu, Liangxue Lai, Zhanjun Li · 2022 · Journal of genetics and genomics/Journal of Genetics and Genomics
- Gene Editing in Hematopoietic Stem CellsJiaoyang Liao, Yuxuan Wu · 2023 · Advances in experimental medicine and biology
- CRISPR/Cas systems and techniquesAnuj Kumar Poonia, Bhavika Batra, Milan Kumar Lal, Vijay Gahlaut · 2024 · Elsevier eBooks
- Role of CRISPR-Cas and Its Application in Mitigating Plant StressSharmistha Ganguly, Akshata G. Athreya, Dhavalkumar Patel · 2024 · Springer eBooks
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- Application of CRISPR-Cas Technique in Enhancing the Phytochemicals ProductionHarshita Sahoo, Manisha Malhotra, Vikas Kumar Yadav, Vikash Maurya, Shweta ., Akhilesh Kumar · 2024 · BENTHAM SCIENCE PUBLISHERS eBooks
- CRISPR-Cas9 for therapy: the challenges and ways to overcome themSundaram Acharya, Souvik Maiti, Debojyoti Chakraborty · 2020 · Elsevier eBooks
- Functional Assessment of BRCA1 variants using CRISPR-Mediated Base EditorsJi-Eun See, Ha Rim Shin, Gayoung Jang, Jiyeon Kweon, Yongsub Kim · 2021 · Journal of Visualized Experiments
- Applications of CRISPR-Cas System in Tumor BiologyMengdan Ma, Yuchen Liu, Weiren Huang · 2021 · ONCOLOGIE
- CRISPR Genome Editing: Into the Second DecadeGeulah Livshits · 2022 · GEN Biotechnology
- Expanding the Scope of Base Editing in Crops Using Cas9 VariantsRukmini Mishra, Muntazir Mushtaq, Raj Kumar Joshi · 2022 · Springer eBooks
- Multiplexed Genome Editing in Plants Using CRISPR/Cas-Based Endonuclease SystemsNagaveni Budhagatapalli, Göetz Hensel · 2022 · Springer eBooks
- Introduction and Perspectives of DNA Base EditorsBeomjong Song, Sangsu Bae · 2023 · Methods in molecular biology
- Expanding the RNA- and RNP-Based Regulatory World in Mammalian CellsShunsuke Kawasaki, Moe Hirosawa, Hirohide Saito · 2023 · Springer eBooks
- Discovery and engineering of Tsp2Cas9 for genome editingHuilin Mao, Yuwen Tian, Ziwen Wang, Jingtong Liu, Jingjing Wei, Yao Wang, Tao Chen, Miaomiao Li, Shengzhou Wang, Li Shen et al. · 2024 · Cell Discovery
- CRISPR-guided programmable self-assembly of artificial virus-like nucleocapsidsCarlos Calcines-Cruz, Ilya J. Finkelstein, Armando T. Hernández-García · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
- Preface: More than two decades of modern tumor immunologyLorenzo Galluzzi, Nils-Petter Rudqvist · 2019 · Methods in enzymology on CD-ROM/Methods in enzymology
- Preface: More than two decades of modern tumor immunologyLorenzo Galluzzi, Nils-Petter Rudqvist · 2020 · Methods in enzymology on CD-ROM/Methods in enzymology
- SafeChassis: engineering biosafety for industrial biotechnologyEnrique Asin‐Garcia · 2022
- Minimized combinatorial CRISPR screens identify genetic interactions in autophagyValentina Diehl · 2022 · Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt)
- Advancement in Delivery Systems and Vector Selection for CRISPR/Cas‐Mediated Genome Editing in PlantsSanskriti Vats, Sukhmandeep Kaur, Amit Chauhan, Dipul Kumar Biswas, Rupesh Kailasrao Deshmukh · 2023 · Wiley eBooks
- Mitigating Heat Stress Response in CRISPR/Cas‐Mediated Edited Crops by Altering the Expression Pattern of Noncoding DNAEmanpreet Kaur, Louie Cris Lopos, Andriy Bilichak · 2025 · Wiley eBooks
- DeepEmbCas9: Cas9 coevolution and sgRNA structural information for CRISPR-Cas9 cleavage activity predictionJeffrey Kelvin Mak, Péter Mináry · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- Single residues within the YTHDF intrinsically disordered region control m 6 A-dependent mRNA decayBenedetta Ricci, Danika Sommer, Daymieri Narvaez, Ruqi Zhang, Jacob B. Geri, Sara Zaccara · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
- Pin-point base editing for next generation breedingKeiji Nishida · 2019 · Japanese Journal of Pesticide Science
- Modelling Niemann-Pick disease type C in a human haploid cell line allows for patient variant characterization and clinical interpretationSteven Erwood, Reid A. Brewer, Teija M.I. Bily, Eleonora Maino, Liangchi Zhou, Ronald D. Cohn, Evgueni A. Ivakine · 2019 · bioRxiv (Cold Spring Harbor Laboratory)
- Application of CRISPR-Cas9 gene editing to treat HBVKun Yan, Yu Chen, Jiangpeng Feng, Yong Xiong · 2019 · Chinese Science Bulletin (Chinese Version)
- Targeted genome editing using CRISPR/Cas9 system in fungiTakayuki Arazoe, Osamu Mizutani · 2020 · Elsevier eBooks
- Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scopeLukas Villiger, Lukas Schmidheini, Nicolas Mathis, Tanja Rothgangl, Kim Fabiano Marquart, Gerald Schwank · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
- Efficient GNE myopathy disease modeling with mutation specific phenotypes in human pluripotent stem cells by base editorsJu-Chan Park, Jumee Kim, Hyun-Ki Jang, Seung-Yeon Lee, Keun-Tae Kim, Seokwoo Park, Hyun Sik Lee, Hee‐Jung Choi, Soon‐Jung Park, Sung‐Hwan Moon et al. · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
- Establishment of an efficient genotyping technique based on targeted DNA endonuclease in vitro activity of CRISPR/Cas9 ribonucleoproteinNan Wang, Xiantao Qi, Changlin Liu, Chuanxiao Xie, Jinjie Zhu · 2020 · ACTA AGRONOMICA SINICA
- Full-Spectrum Targeted Mutagenesis in Plant and Animal CellsBrian J. Iaffaldano, Jakob Reiser · 2021 · International Journal of Molecular Sciences
- Choosing a nuclease, guide RNA, and repair templateKiran Musunuru · 2021 · Elsevier eBooks
- Optimizing streptomyces sp. MD102 for heterologous expression of the cannabinoid biosynthetic pathwayRachel Yuen Fong Chea · 2020 · DR-NTU (Nanyang Technological University)
- Genetic engineering in plants using CRISPRsAndriy Bilichak · 2021 · Elsevier eBooks
- Chassis engineering of E. coli Nissle 1917 for development of live biotherapeuticsHalimatun Sakdiah binti Zainuddin · 2021
- CRISPR/CAS: do sistema imune de procariotos ao controle de doenças de plantasLeila Lourenço Furtado, Camila M. Rêgo-Machado, Gustavo Adolfo Marín-Ramírez, Natália Chagas Freitas, Hugo Bruno Correa Molinari, Thaís Ribeiro Santiago · 2021 · Revisão Anual de Patologia de Plantas
- CRISPR-mediated dense mutagenesis: a tool for rational targeting of multiprotein complexes and the noncoding genomeFalak Sher · 2021 · Elsevier eBooks
- 神先先生の古稀及び定年を迎えられるにあたって (神先秀雄・薄田桂・友松芳郎三教授古稀記念特輯)宏 井上 · 1981 · 関西大学社会学部紀要
- Determination of the Length of Target Recognition Sequence in sgRNA Required for CRISPR InterferenceBum-Joon Kim, Byeong Chan Kim, Ho Joung Lee, Sang Jun Lee · 2021 · Microbiology and Biotechnology Letters
- Faculty Opinions recommendation of Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.Yousin Suh · 2020 · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
- Preface: More than two decades of modern tumor immunologyLorenzo Galluzzi, Nils-Petter Rudqvist · 2020 · Methods in enzymology on CD-ROM/Methods in enzymology
- Preface: More than two decades of modern tumor immunologyLorenzo Galluzzi, Nils-Petter Rudqvist · 2020 · Methods in enzymology on CD-ROM/Methods in enzymology
- Development and Vision of CRISPR-Based TechnologyZiheng Zhang, Ping Wang, Ji‐Long Liu · 2022 · Springer eBooks
- Extension and Improvement of CRISPR-Based TechnologyZiheng Zhang, Ping Wang, Ji‐Long Liu · 2022 · Springer eBooks
- CRISPR CasSaša Šviković · 2022 · Wiley eBooks
- Computational Tools for Target Design and AnalysisGue‐Ho Hwang, Sangsu Bae · 2022 · Wiley eBooks
- DNA Base Editing Strategies for Genome EditingMatthew A. Coelho, Songyuan Li, Ben Taylor · 2022 · Wiley eBooks
- Emerging Technologies for Genome EditingMartin Peterka · 2022 · Wiley eBooks
- Biology of Rice (Oryza sativa)OECD · 2022 · Harmonisation of regulatory oversight in biotechnology
- ForewordOECD · 2022 · Harmonisation of regulatory oversight in biotechnology
- Revised points to consider on plant biology consensus documentsOECD · 2022 · Harmonisation of regulatory oversight in biotechnology
- Executive summaryOECD · 2022 · Harmonisation of regulatory oversight in biotechnology
- Faculty Opinions recommendation of Expanding C-T base editing toolkit with diversified cytidine deaminases.Yousin Suh · 2020 · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
- CRISPR/Cas rendszerek és kis RNS-eik expressziójára alkalmazott promóterek vizsgálataNóra Weinhardt · 2021 · SZTE Repository of Dissertations (University of Szeged)
- How to increase precision in Xenopus gene editing and base editing experimentsStuart J. Smith · 2021 · OSF Preprints (OSF Preprints)
- Traditional and Novel Testing Methods for COVID-19Siyu Yu · 2022 · International Conference on Biotechnology, Life Science and Medical Engineering (BLSME)
- Genome Editing Tools for Potato ImprovementKarl Ravet, François Sevestre, Laura Chauvin, Jean-Éric Chauvin, Gisèle Lairy-Joly, Andrew Katz, Pierre Devaux, Nicolas Szydlowski, Jean‐Luc Gallois, Stephen P. Pearce et al. · 2022 · Springer eBooks
- Quantitative assaying of SpCas9-NG with fluorescent reportersAlexandre Baccouche, Kévin Montagne, Nozomu Yachie, Teruo Fujii, Anthony J. Genot · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Discovery and biosynthesis of specialized metabolites produced by an indigenous streptomyces strainHartono Candra · 2022
- Molecular Mechanisms of Type II CRISPR ‐ Cas SystemsTautvydas Karvelis, Virginijus Šikšnys · 2022 · Wiley eBooks
- Genome Editing with CRISPR‐Cas SystemsPeter Lotfy, Patrick D. Hsu · 2022 · Wiley eBooks
- Gene editing hPSCs for modeling neurological disordersAndrew J. Petersen · 2022 · Elsevier eBooks
- CRISPR/Cas9, a decade of genome editing tools to fix the DNAL. Santos · 2022 · Science Reviews Biology
- Plant Genome EditingNaoki Wada, Yuriko Osakabe, Keishi Osakabe · 2022 · CABI eBooks
- New Cas Endonuclease Variants Broadening the Scope of the CRISPR ToolboxGöetz Hensel · 2022 · Springer eBooks
- Recent Advances and Application of CRISPR Base Editors for Improvement of Various Traits in CropsP. Sushree Shyamli, Sandhya Suranjika, Seema Pradhan, Ajay Kumar Parida · 2022 · Springer eBooks
- Data from Efficient Correction of Oncogenic KRAS and TP53 Mutations through CRISPR Base EditingShady Sayed, Olga Alexandra Sidorova, Alexander Hennig, Martina Augsburg, Catherine P. Cortés Vesga, Moustafa Abohawya, Lukas Theo Schmitt, Duran Sürün, Daniel E. Stange, Jovan Mircetic et al. · 2023
- Gene and base editing tools to accelerate cereal improvementΝικόλαος Τσακιρπάλογλου, Kurniawan Rudi Trijatmiko, Endang M. Septiningsih, Michael J. Thomson · 2023 · Elsevier eBooks
- Expanding the RNA and RNP-Based Regulatory World in Mammalian CellsShunsuke Kawasaki, Moe Hirosawa, Hirohide Saito · 2023 · Springer eBooks
- Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 SystemMeng-Fei Xu, Jie Chen, Yue Xu, Jing-Lian Zhang, Yi Zhou, Jie‐Jie He, Shan Wu, Ya‐Lan Wei, Zhen‐Yu She · 2023 · Journal of Visualized Experiments
- Genome Editing for Microbial Pathogens Resistance in CropsMudasir Ahmad Bhat, Saima Jan, Sumreen Amin Shah, Arif Tasleem Jan · 2023 · Wiley eBooks
- eLife Assessment: Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington's diseaseGene Wei-Ming Yeo · 2023 · eLife
- Reviewer #3 (Public Review): Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington's disease2023 · eLife
- History, Development and Characteristics of CRISPR-Cas SystemTaha Nazir, Hameed A. Mirza, Nida Taha · 2024 · Preprints.org
- Applications and associated challenges of CRISPR-Cas technology in agricultureSangeeta Rathore, Rohi Bhatt, Swati M. Joshi, Kavya Ajit Pandya, Neeru Singh · 2024 · Elsevier eBooks
- Gene Editing Approaches for Haematological DisordersSamuele Ferrari, Daniele Canarutto · 2024 · Elsevier eBooks
- CRISPR-based precision breeding of fruits, vegetables, and ornamental plantsMohamed A. M. Atia, Arwa Salah, Aisha Elsawah, Ahmed Karam, Taghreed Khaled Abdelmoneim · 2024 · Elsevier eBooks
- Additional file 3 of Knockout of circRNAs by base editing back-splice sites of circularized exonsXiang Gao, Xu-Kai Ma, Xiang Li, Guo‐Wei Li, Chu‐Xiao Liu, Jun Zhang, Ying Wang, Jia Wei, Jia Chen, Ling‐Ling Chen et al. · 2022 · Figshare
- Additional file 2 of Knockout of circRNAs by base editing back-splice sites of circularized exonsXiang Gao, Xu-Kai Ma, Xiang Li, Guo‐Wei Li, Chu‐Xiao Liu, Jun Zhang, Ying Wang, Jia Wei, Jia Chen, Ling‐Ling Chen et al. · 2022 · Figshare
- Author response: Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington’s diseaseDoo Eun Choi, Jun Wan Shin, Sophia Zeng, Eun Pyo Hong, Jae-Hyun Jang, Jacob M. Loupe, Vanessa C. Wheeler, Hannah E. Stutzman, Ben Kleinstiver, Jong‐Min Lee · 2024 · eLife