Engineered CRISPR-Cas9 nuclease with expanded targeting space

Coot : model-building tools for molecular graphics

Emsley, P., & Cowtan, K. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904019158 · Cited by 30,571

PHENIX : a comprehensive Python-based system for macromolecular structure solution

Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

XDS

Kabsch, W. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909047337 · Cited by 16,463

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

DNA targeting specificity of RNA-guided Cas9 nucleases

Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741

How good are my data and what is the resolution?

Evans, P. R., & Murshudov, G. N. (2013) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444913000061 · Cited by 4,713

Molecular replacement with MOLREP

Vagin, A. A., & Teplyakov, A. (2009) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909042589 · Cited by 3,580

Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2

Sangree, A. K., Griffith, A. L., Szegletes, Z. M., Roy, P., DeWeirdt, P. C., Hegde, M., McGee, A. V., Hanna, R. E., & Doench, J. G. (2022) · Nature Communications · doi:10.1038/s41467-022-28884-7 · Cited by 64

Recent advances in CRISPR-based functional genomics for the study of disease-associated genetic variants

Kim, H. S., Kweon, J., & Kim, Y. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01212-3 · Cited by 68

Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing

Li, J., Hong, S., Chen, W., Zuo, E., & Yang, H. (2019) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2019.11.002 · Cited by 66

CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Guo, M., Chen, H., Dong, S., Zhang, Z., & Luo, H. (2022) · Chinese Medicine · doi:10.1186/s13020-022-00584-w · Cited by 66

A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies

Hoberecht, L., Perampalam, P., Lun, A. T. L., & Fortin, J. (2022) · Nature Communications · doi:10.1038/s41467-022-34320-7 · Cited by 66

Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9

Hibshman, G. N., Bravo, J. P. K., Hooper, M. M., Dangerfield, T. L., Zhang, H., Finkelstein, I. J., Johnson, K. A., & Taylor, D. W. (2024) · Nature Communications · doi:10.1038/s41467-024-47830-3 · Cited by 70

Recent advances in therapeutic gene-editing technologies

Liu, D., Cao, D., & Han, R. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.03.026 · Cited by 70

Gene disruption through base editing‐induced messenger RNA missplicing in plants

Li, Z., Xiong, X., Wang, F., Liang, J., & Li, J. (2018) · New Phytologist · doi:10.1111/nph.15647 · Cited by 62

Assigning functionality to cysteines by base editing of cancer dependency genes

Li, H., Ma, T., Remsberg, J. R., Won, S. J., DeMeester, K. E., Njomen, E., Ogasawara, D., Zhao, K. T., Huang, T. P., Lu, B., et al. (2023) · Nature Chemical Biology · doi:10.1038/s41589-023-01428-w · Cited by 62

Multiple gene substitution by Target-AID base-editing technology in tomato

Hunziker, J., Nishida, K., Kondo, A., Kishimoto, S., Ariizumi, T., & Ezura, H. (2020) · Scientific Reports · doi:10.1038/s41598-020-77379-2 · Cited by 61

Prediction of CRISPR/Cas9 single guide RNA cleavage efficiency and specificity by attention-based convolutional neural networks

Zhang, G., Zeng, T., Dai, Z., & Dai, X. (2021) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2021.03.001 · Cited by 64

Functional correction of CFTR mutations in human airway epithelial cells using adenine base editors

Krishnamurthy, S., Traore, S., Cooney, A. L., Brommel, C. M., Kulhánková, K., Sinn, P. L., Newby, G. A., Liu, D. R., & McCray, P. B. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab788 · Cited by 63

Visual function restoration in a mouse model of Leber congenital amaurosis via therapeutic base editing

Jo, D. H., Jang, H., Cho, C. S., Han, J. H., Ryu, G., Jung, Y., Bae, S., & Kim, J. H. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.11.021 · Cited by 61

GO: a functional reporter system to identify and enrich base editing activity

Katti, A., Foronda, M., Zimmerman, J., Diaz, B. J., Zafra, M. P., Goswami, S., & Dow, L. E. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa124 · Cited by 60

Therapeutic base editing and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosa

Hong, S.-A., Kim, S., Lee, A.-Y., Hwang, G., Kim, J. H., Iwata, H., Kim, S., Bae, S., & Lee, S. E. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.06.005 · Cited by 64

SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish

Liang, F., Zhang, Y., Li, L., Yang, Y., Fei, J., Liu, Y., & Qin, W. (2022) · Nature Communications · doi:10.1038/s41467-022-31034-8 · Cited by 61

Engineered Cas9 variant tools expand targeting scope of genome and base editing in rice

Zeng, D., Li, X., Huang, J., Li, Y., Cai, S., Yu, W., Li, Y., Huang, Y., Xie, X., Gong, Q., et al. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13293 · Cited by 60

The rapidly advancing Class 2 CRISPR‐Cas technologies: A customizable toolbox for molecular manipulations

Wang, J., Zhang, C., & Feng, B. (2020) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.15039 · Cited by 61

PAM-flexible genome editing with an engineered chimeric Cas9

Zhao, L., Koseki, S. R. T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C., Stan, T., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41829-y · Cited by 59

In situ CRISPR‐Cas9 base editing for the development of genetically engineered mouse models of breast cancer

Annunziato, S., Lutz, C., Henneman, L., Bhin, J., Wong, K., Siteur, B., van Gerwen, B., de Korte‐Grimmerink, R., Zafra, M. P., Schatoff, E. M., et al. (2020) · The EMBO Journal · doi:10.15252/embj.2019102169 · Cited by 62

Integrating machine learning and genome editing for crop improvement

Chen, L., Liu, G., & Zhang, T. (2024) · aBIOTECH · doi:10.1007/s42994-023-00133-5 · Cited by 60

Epigenome editing for targeted DNA (de)methylation: a new perspective in modulating gene expression

Seem, K., Kaur, S., Kumar, S., & Mohapatra, T. (2024) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409238.2024.2320659 · Cited by 59

Engineered xCas9 and SpCas9‐NG variants broaden PAM recognition sites to generate mutations in Arabidopsis plants

Ge, Z., Zheng, L., Zhao, Y., Jiang, J., Zhang, E. J., Liu, T., Gu, H., & Qu, L. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13148 · Cited by 61

Expanding the CRISPR Toolbox in P. patens Using SpCas9-NG Variant and Application for Gene and Base Editing in Solanaceae Crops

Veillet, F., Perrot, L., Guyon‐Debast, A., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., Mazier, M., & Nogué, F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031024 · Cited by 59

An adenine base editor with expanded targeting scope using SpCas9‐NG v1 in rice

Negishi, K., Kaya, H., Abe, K., Hara, N., Saika, H., & Toki, S. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13120 · Cited by 57

Delivery of nucleic acid based genome editing platforms via lipid nanoparticles: Clinical applications

Masarwy, R., Stotsky‐Oterin, L., Elisha, A., Hazan‐Halevy, I., & Peer, D. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115359 · Cited by 59

Multiplex Gene Disruption by Targeted Base Editing of Yarrowia lipolytica Genome Using Cytidine Deaminase Combined with the CRISPR/Cas9 System

Bae, S., Park, B. G., Kim, B., & Hahn, J. (2019) · Biotechnology Journal · doi:10.1002/biot.201900238 · Cited by 57

Super-Treg: Toward a New Era of Adoptive Treg Therapy Enabled by Genetic Modifications

Amini, L., Greig, J., Schmueck‐Henneresse, M., Volk, H., Bézie, S., Reinke, P., Guillonneau, C., Wagner, D. L., & Anegón, I. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2020.611638 · Cited by 58

Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice

Wu, Y., Ren, Q., Zhong, Z., Liu, G., Han, Y., Bao, Y., Liu, L., Xiang, S., Liu, S., Tang, X., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13838 · Cited by 56

Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

Rose, J. C., Popp, N. A., Richardson, C. D., Stephany, J. J., Mathieu, J., Wei, C. T., Corn, J. E., Maly, D. J., & Fowler, D. M. (2020) · Nature Communications · doi:10.1038/s41467-020-16542-9 · Cited by 61

Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing

Liu, R., Liang, L., Freed, E. F., & Gill, R. T. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.07.005 · Cited by 58

Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression

Cheng, L., Li, Y., Qi, Q., Xu, P., Feng, R., Palmer, L. E., Chen, J., Wu, R., Yee, T., Zhang, J., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00861-8 · Cited by 59

Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods

Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., Ying, W., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25217-y · Cited by 55

Methods and applications of CRISPR/Cas system for genome editing in stem cells

Yang, G., & Huang, X. (2019) · Cell Regeneration · doi:10.1016/j.cr.2019.08.001 · Cited by 55

Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases

Wang, W., Tian, B., Pan, Q., Chen, Y., He, F., Bai, G., Akhunova, A. R., Trick, H. N., & Akhunov, E. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13669 · Cited by 57

DNA base editing in nuclear and organellar genomes

Tan, J., Forner, J., Karcher, D., & Bock, R. (2022) · Trends in Genetics · doi:10.1016/j.tig.2022.06.015 · Cited by 57

High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes

Ikeda, A., Fujii, W., Sugiura, K., & Naito, K. (2019) · Communications Biology · doi:10.1038/s42003-019-0627-8 · Cited by 57

CRISPR technologies for the treatment of Duchenne muscular dystrophy

Choi, E., & Koo, T. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.04.002 · Cited by 57

Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing

Rabinowitz, R., & Offen, D. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.11.009 · Cited by 58

Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system

Zhong, Z., Liu, G., Tang, Z., Xiang, S., Yang, L., Huang, L., He, Y., Fan, T., Liu, S., Zheng, X., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41802-9 · Cited by 58

SpCas9-NG self-targets the sgRNA sequence in plant genome editing

Qin, R., Li, J., Liu, X., Xu, R., Yang, J., & Wei, P. (2020) · Nature Plants · doi:10.1038/s41477-020-0603-9 · Cited by 55

Base editing strategy for insertion of the A673T mutation in the APP gene to prevent the development of AD in vitro

Guyon, A., Rousseau, J., Bégin, F.-G., Bertin, T., Lamothe, G., & Tremblay, J. P. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.02.032 · Cited by 55

CRISPR mediated genome engineering to develop climate smart rice: Challenges and opportunities

Biswal, A. K., Mangrauthia, S. K., Reddy, M. R., & Yugandhar, P. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.04.005 · Cited by 56

Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

Liu, Z., Chen, S., Shan, H., Jia, Y., Chen, M., Song, Y., Lai, L., & Li, Z. (2020) · BMC Biology · doi:10.1186/s12915-020-00849-6 · Cited by 53

A FLASH pipeline for arrayed CRISPR library construction and the gene function discovery of rice receptor-like kinases

Chen, K., Ke, R., Du, M., Yi, Y., Chen, Y., Wang, X., Lu, Y., Liu, H., Hou, X., Xiong, L., et al. (2021) · Molecular Plant · doi:10.1016/j.molp.2021.09.015 · Cited by 54

CRISPR-derived genome editing technologies for metabolic engineering

Nishida, K., & Kondo, A. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2020.12.002 · Cited by 54

Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences

Sretenovic, S., Yin, D., Levav, A., Selengut, J., Mount, S. M., & Qi, Y. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100101 · Cited by 53

Recent Progress and Challenges in CRISPR-Cas9 Engineered Algae and Cyanobacteria

Patel, V. K., Das, A., Kumari, R., & Kajla, S. (2023) · Algal Research · doi:10.1016/j.algal.2023.103068 · Cited by 50

A CRISPR way for accelerating cereal crop improvement: Progress and challenges

Basu, U., Riaz Ahmed, S., Bhat, B. A., Anwar, Z., Ali, A., Ijaz, A., Gulzar, A., Bibi, A., Tyagi, A., Nebapure, S. M., et al. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2022.866976 · Cited by 51

Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9

Jeong, Y. K., Yu, J., & Bae, S. (2019) · Scientific Reports · doi:10.1038/s41598-019-41356-1 · Cited by 51

Gene-Editing Technologies Paired With Viral Vectors for Translational Research Into Neurodegenerative Diseases

Rittiner, J. E., Moncalvo, M., Chiba‐Falek, O., & Kantor, B. (2020) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2020.00148 · Cited by 53

Disruption of HIV-1 co-receptors CCR5 and CXCR4 in primary human T cells and hematopoietic stem and progenitor cells using base editing

Knipping, F., Newby, G. A., Eide, C. R., McElroy, A. N., Nielsen, S. C., Smith, K. D., Fang, Y., Cornu, T. I., Costa, C., Gutiérrez-Guerrero, A., et al. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.10.026 · Cited by 53

Recent Advances in Genome-Editing Technology with CRISPR/Cas9 Variants and Stimuli-Responsive Targeting Approaches within Tumor Cells: A Future Perspective of Cancer Management

Allemailem, K. S., Almatroodi, S. A., Almatroudi, A., Alrumaihi, F., Abdulmonem, W. A., Al‐Megrin, W. A. I., Aljamaan, A. N., Rahmani, A. H., & Khan, A. A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24087052 · Cited by 53

In vivo prime editing rescues alternating hemiplegia of childhood in mice

Sousa, A. A., Terrey, M., Sakai, H. A., Simmons, C. Q., Arystarkhova, E., Morsci, N. S., Anderson, L. C., Xie, J., Suri‐Payer, F., Laux, L. C., et al. (2025) · Cell · doi:10.1016/j.cell.2025.06.038 · Cited by 54

Impact of CRISPR interference on strain development in biotechnology

Schultenkämper, K., Brito, L. F., & Wendisch, V. F. (2020) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1901 · Cited by 57

In the business of base editors: Evolution from bench to bedside

Porto, E. M., & Komor, A. C. (2023) · PLoS Biology · doi:10.1371/journal.pbio.3002071 · Cited by 53

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

Song, L., & Xie, K. (2020) · Microbiome · doi:10.1186/s40168-020-00859-0 · Cited by 53

De Novo Engineering of Corynebacterium glutamicum for l ‑Proline Production

Zhang, J., Qian, F., Dong, F., Wang, Q., Yang, J., Jiang, Y., & Yang, S. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.0c00249 · Cited by 50

Massively parallel assessment of human variants with base editor screens

Hanna, R. E., Hegde, M., Fagre, C. R., DeWeirdt, P. C., Sangree, A. K., Szegletes, Z., Griffith, A., Feeley, M. N., Sanson, K. R., Baidi, Y., et al. (2020) · doi:10.1101/2020.05.17.100818 · Cited by 314

CRISPR‐Cas9 Based Genome Editing in Wheat

Smedley, M. A., Hayta, Ş., Clarke, M., & Harwood, W. (2021) · Current Protocols · doi:10.1002/cpz1.65 · Cited by 51

Efficient Correction of Oncogenic KRAS and TP53 Mutations through CRISPR Base Editing

Sayed, S., Sidorova, O. A., Hennig, A., Augsburg, M., Vesga, C. P. C., Abohawya, M., Schmitt, L. T., Sürün, D., Stange, D. E., Mircetic, J., et al. (2022) · Cancer Research · doi:10.1158/0008-5472.can-21-2519 · Cited by 51

Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB

Lampson, B. L., Ramίrez, A. S., Baro, M., He, L., Hegde, M., Koduri, V., Pfaff, J. L., Hanna, R. E., Kowal, J., Shirole, N. H., et al. (2024) · Cell · doi:10.1016/j.cell.2024.03.022 · Cited by 49

CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis

Schoonenberg, V. A. C., Cole, M. A., Yao, Q., Macias-Treviño, C., Sher, F., Schupp, P. G., Canver, M. C., Maeda, T., Pinello, L., & Bauer, D. E. (2018) · Genome biology · doi:10.1186/s13059-018-1563-5 · Cited by 49

Expanding the scope of CRISPR/Cas9‐mediated genome editing in plants using an xCas9 and Cas9‐NG hybrid

Niu, Q., Wu, S., Li, Y., Yang, X., Liu, P., Xu, Y., & Lang, Z. (2019) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12886 · Cited by 51

Catalytic-state structure and engineering of Streptococcus thermophilus Cas9

Zhang, Y., Zhang, H., Xu, X., Wang, Y., Chen, W., Wang, Y., Wu, Z., Tang, N., Wang, Y., Zhao, S., et al. (2020) · Nature Catalysis · doi:10.1038/s41929-020-00506-9 · Cited by 49

Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing

Chen, Y., Hu, Y., Wang, X., Luo, S., Yang, N., Chen, Y., Li, Z., Zhou, Q., & Li, W. (2022) · The Innovation · doi:10.1016/j.xinn.2022.100264 · Cited by 49

New Possibilities on the Horizon: Genome Editing Makes the Whole Genome Accessible for Changes

Kawall, K. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00525 · Cited by 50

Recent developments and applications of genetic transformation and genome editing technologies in wheat

Wang, K., Gong, Q., & Ye, X. (2019) · Theoretical and Applied Genetics · doi:10.1007/s00122-019-03464-4 · Cited by 49

Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

Zhao, Y., Tian, J., Zheng, G., Chen, J., Sun, C., Yang, Z., Zimin, A. A., Jiang, W., Deng, Z., Wang, Z., et al. (2019) · Science China Life Sciences · doi:10.1007/s11427-019-1559-y · Cited by 50

Base Editing: The Ever Expanding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Tool Kit for Precise Genome Editing in Plants

Monsur, M. B., Shao, G., Lv, Y., Ahmad, S., Wei, X., Hu, P., & Tang, S. (2020) · Genes · doi:10.3390/genes11040466 · Cited by 48

Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees

Chen, Z., Traniello, I. M., Rana, S., Cash-Ahmed, A. C., Sankey, A. L., Yang, C., & Robinson, G. E. (2021) · Journal of Neurogenetics · doi:10.1080/01677063.2021.1887173 · Cited by 52

Benchmarking deep learning methods for predicting CRISPR/Cas9 sgRNA on- and off-target activities

Zhang, G., Luo, Y., Dai, X., & Dai, Z. (2023) · Briefings in Bioinformatics · doi:10.1093/bib/bbad333 · Cited by 47

Gene Editing of Hematopoietic Stem Cells: Hopes and Hurdles Toward Clinical Translation

Ferrari, S., Vavassori, V., Canarutto, D., Jacob, A., Castiello, M. C., Omer, A., & Genovese, P. (2021) · Frontiers in Genome Editing · doi:10.3389/fgeed.2021.618378 · Cited by 52

CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement

Zegeye, W. A., Tsegaw, M., Zhang, Y., & Cao, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23084454 · Cited by 49

Efficient base editing with high precision in rabbits using YFE-BE4max

Liu, Z., Chen, S., Shan, H., Jia, Y., Chen, M., Song, Y., Lai, L., & Li, Z. (2020) · Cell Death and Disease · doi:10.1038/s41419-020-2244-3 · Cited by 47

Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction

Huang, C., Li, G., Wu, J., Liang, J., & Wang, X. (2021) · Genome biology · doi:10.1186/s13059-021-02305-2 · Cited by 48

Plant genome editing: ever more precise and wide reaching

Sukegawa, S., Saika, H., & Toki, S. (2021) · The Plant Journal · doi:10.1111/tpj.15233 · Cited by 48

Highly efficient base editing with expanded targeting scope using SpCas9‐NG in rabbits

Liu, Z., Shan, H., Chen, S., Chen, M., Song, Y., Lai, L., & Li, Z. (2019) · The FASEB Journal · doi:10.1096/fj.201901587r · Cited by 46

Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture

Wang, G., Wang, F., Xu, Z., Wang, Y., Zhang, C., Zhou, Y., Hui, F., Yang, X., Nie, X., Zhang, X., et al. (2024) · Genome biology · doi:10.1186/s13059-024-03189-8 · Cited by 46

Next Generation Precision Medicine: CRISPR-mediated Genome Editing for the Treatment of Neurodegenerative Disorders

Raikwar, S. P., Kikkeri, N. S., Sakuru, R. C., Saeed, D., Zahoor, H., Premkumar, K., Mentor, S., Thangavel, R., Dubova, I., Ahmed, M. E., et al. (2019) · Journal of Neuroimmune Pharmacology · doi:10.1007/s11481-019-09849-y · Cited by 48

beditor : A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing

Dandage, R., Després, P. C., Yachie, N., & Landry, C. R. (2019) · Genetics · doi:10.1534/genetics.119.302089 · Cited by 46

Microbial CRISPRi and CRISPRa Systems for Metabolic Engineering

Zheng, Y., Su, T., & Qi, Q. (2019) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-019-0107-5 · Cited by 46

Ocular delivery of CRISPR/Cas genome editing components for treatment of eye diseases

Yu, W., & Wu, Z. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.011 · Cited by 48

Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes

Vicencio, J., Sánchez-Bolaños, C., Moreno-Sánchez, I., Brena, D., Vejnar, C. E., Kukhtar, D., Ruiz-López, M., Cots-Ponjoan, M., Rubio, A., Melero, N. R., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-30228-4 · Cited by 47

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′ G-extended sgRNAs

Kulcsár, P. I., Tálas, A., Tóth, E., Nyeste, A., Ligeti, Z., Welker, Z., & Welker, E. (2020) · Nature Communications · doi:10.1038/s41467-020-15021-5 · Cited by 45

An adenine base editor variant expands context compatibility

Xiao, Y., Wu, Y., & Tang, W. (2024) · Nature Biotechnology · doi:10.1038/s41587-023-01994-3 · Cited by 48

Precise Genome Editing in Poultry and Its Application to Industries

Park, J. S., Lee, K. Y., & Han, J. Y. (2020) · Genes · doi:10.3390/genes11101182 · Cited by 46

Engineering of efficiency-enhanced Cas9 and base editors with improved gene therapy efficacies

Yin, S., Zhang, M., Liu, Y., Sun, X., Guan, Y., Chen, X., Yang, L., Huo, Y., Yang, J., Zhang, X., et al. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.11.014 · Cited by 47

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

May, D., Paldi, K., & Altpeter, F. (2023) · The Plant Genome · doi:10.1002/tpg2.20298 · Cited by 47

PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics

Acharya, S., Ansari, A. H., Das, P. K., Hirano, S., Aich, M., Rauthan, R., Mahato, S., Maddileti, S., Sarkar, S., Kumar, M., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49233-w · Cited by 47

CRISPR-Pass: Gene Rescue of Nonsense Mutations Using Adenine Base Editors

Lee, C., Jo, D. H., Hwang, G., Yu, J., Kim, J., Park, S.-E., Kim, J., Kim, J. H., & Bae, S. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.05.013 · Cited by 47

Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding

Okafor, I. C., Singh, D., Wang, Y., Jung, M., Wang, H., Mallon, J. P., Bailey, S., Lee, J. K., & Ha, T. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz1058 · Cited by 45

CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus

Kuroiwa, K., Thenault, C., Nogué, F., Perrot, L., Mazier, M., & Gallois, J. (2021) · Plant Science · doi:10.1016/j.plantsci.2021.111160 · Cited by 44

The CRISPR/Cas System: A Customizable Toolbox for Molecular Detection

He, Y., Yan, W., Long, L., Dong, L., Ma, Y., Li, C., Xie, Y., Liu, N., Xing, Z., Xia, W., et al. (2023) · Genes · doi:10.3390/genes14040850 · Cited by 47

Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

Grajcarek, J., Monlong, J., Nishinaka‐Arai, Y., Nakamura, M., Nagai, M., Matsuo, S., Lougheed, D. R., Sakurai, H., Saito, M. K., Bourque, G., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-12829-8 · Cited by 45

Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease

Qi, T., Wu, F., Xie, Y., Gao, S., Li, M., Pu, J., Li, D., Lan, F., & Wang, Y. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.590581 · Cited by 45

Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide

Liu, Z., Chen, S., Jia, Y., Shan, H., Chen, M., Song, Y., Lai, L., & Li, Z. (2021) · Science China Life Sciences · doi:10.1007/s11427-020-1775-2 · Cited by 35

Novel Wx alleles generated by base editing for improvement of rice grain quality

Huang, X., Su, F., Huang, S., Mei, F., Niu, X., Ma, C., Zhang, H., Zhu, X., Zhu, J., & Zhang, J. (2021) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13098 · Cited by 43

Prime editing primarily induces undesired outcomes in mice

Aida, T., Wilde, J. J., Yang, L., Hou, Y., Li, M., Xu, D., Lin, J., Qi, P., Lu, Z., & Feng, G. (2020) · doi:10.1101/2020.08.06.239723 · Cited by 43

BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity

Wang, P., Xu, L. M., Gao, Y., & Han, R. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.04.009 · Cited by 42

Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM

Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 186

Expanding the targeting space of Cas9

Mao, S. (2018) · Science · doi:10.1126/science.361.6408.1211-g

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science · doi:10.1126/science.aba8853 · Cited by 1,233

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71626-2 · Cited by 2

Functional Characterization and Improved Engineering of the PAM-flexible Cas9 Variant, SpG-Cas9

Limbasia, S. (2023) · Texas Digital Library (University of Texas) · doi:10.26153/tsw/60656