Engineered CRISPR-Cas9 nuclease with expanded targeting space

Coot : model-building tools for molecular graphics

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Multiplex Genome Engineering Using CRISPR/Cas Systems

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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

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DNA targeting specificity of RNA-guided Cas9 nucleases

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How good are my data and what is the resolution?

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Molecular replacement with MOLREP

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Massively parallel CRISPR-assisted homologous recombination enables saturation editing of full-length endogenous genes in yeast

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Navigating the CRISPR/Cas Landscape for Enhanced Diagnosis and Treatment of Wilson’s Disease

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Creating large-scale genetic diversity in Arabidopsis via base editing-mediated deep artificial evolution

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CRISPR/Cas9 and its derivatives to improve crop biotic stress resistance: Current status and prospects

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Prime Editing: Mechanistic Insights and DNA Repair Modulation

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Selecting patients with sickle cell disease for gene addition or gene editing‐based therapeutic approaches: Report on behalf of a joint EHA Specialized Working Group and EBMT Hemoglobinopathies Working Party consensus conference

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Predicting adenine base editing efficiencies in different cellular contexts by deep learning

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Microalgal bioengineering for futuristic applications in synthetic and space biology

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Case study of xylose conversion to glycolate in Corynebacterium glutamicum: Current limitation and future perspective of the CRISPR-Cas systems

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An overview of currently available molecular Cas-tools for precise genome modification

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Precision Genome Engineering Through Cytidine Base Editing in Rapeseed (Brassica napus. L)

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CRISPR technology for abiotic stress resistant crop breeding

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Evolutionary trade‐offs at the Arabidopsis WRR4A resistance locus underpin alternate Albugo candida race recognition specificities

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Improvements in the genetic editing technologies: CRISPR-Cas and beyond

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Nucleases in gene-editing technologies: past and prologue

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Modular vector assembly enables rapid assessment of emerging CRISPR technologies

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Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing

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Improved EGFR mutation detection sensitivity after enrichment by Cas9/sgRNA digestion and PCR amplification

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Genome editing to define the function of risk loci and variants in rheumatic disease

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PRIMA: a rapid and cost-effective genotyping method to detect single-nucleotide differences using probe-induced heteroduplexes

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CRISPR-Cas Technology: A Genome-Editing Powerhouse for Molecular Plant Breeding

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Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length

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How Crisp is CRISPR? CRISPR-Cas-mediated crop improvement with special focus on nutritional traits

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Evaluation and prediction of guide RNA activities in genome-editing tools

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Methods for detecting off-target effects of CRISPR/Cas9

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CRISPR–Cas-Mediated Reprogramming Strategies to Overcome Antimicrobial Resistance

Yoon, B., Kim, J. A., & Kang, Y. K. (2026) · Pharmaceutics · doi:10.3390/pharmaceutics18010095 · Cited by 12

The Evolution of Nucleic Acid–Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design

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Gene Editing in Ganoderma lucidum: Development, Challenges, and Future Prospects

He, S., Liu, Y., Zhang, Z., Cai, M., Hao, Y., & Hu, H. (2025) · Journal of Fungi · doi:10.3390/jof11040310 · Cited by 11

Prediction of CRISPR-Cas9 on-target activity based on a hybrid neural network

Li, C. T., Zou, Q., Li, J., & Feng, H. (2025) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2025.05.001 · Cited by 12

Charting the development and engineering of CRISPR base editors: lessons and inspirations

Zou, S., Sun, Y., & Tang, W. (2025) · Cell chemical biology · doi:10.1016/j.chembiol.2025.05.003 · Cited by 11

Targeted knock-in into the OVA locus of chicken cells using CRISPR/Cas9 system with homology-independent targeted integration

Shi, M., Kawabe, Y., Itô, A., & Kamihira, M. (2019) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2019.09.011 · Cited by 14

Making the cut with PAMless CRISPR-Cas enzymes

Christie, K. A., & Kleinstiver, B. P. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.09.002 · Cited by 11

Innovative cancer nanomedicine based on immunology, gene editing, intracellular trafficking control

Yamada, Y., Sato, Y., Nakamura, T., & Harashima, H. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.05.033 · Cited by 11

Zebrafish Models of Paediatric Brain Tumours

Basheer, F., Dhar, P., & Samarasinghe, R. M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23179920 · Cited by 12

Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays

Nazir, S. (2023) · Talanta Open · doi:10.1016/j.talo.2023.100225 · Cited by 12

Screening an Effective Dual-Adeno-Associated Virus Split-Cytosine Base Editor System for C-to-T Conversion In Vivo

Liu, Q., Chen, Y., Hu, S., Liu, W., Xie, D., Yang, X., Huang, W., Liu, S., Chen, X., Liu, H., et al. (2023) · Human Gene Therapy · doi:10.1089/hum.2023.055 · Cited by 11

Improving CRISPR–Cas9 directed faithful transgene integration outcomes by reducing unwanted random DNA integration

Hermantara, R., Richmond, L., Taqi, A. F., Chilaka, S., Jeantet, V., Guerrini, I., West, K. L., & West, A. G. (2024) · Journal of Biomedical Science · doi:10.1186/s12929-024-01020-x · Cited by 11

Novel CRISPR/SpRY system for rapid detection of CRISPR/Cas-mediated gene editing in rice

Su, Z., Wang, X., Chen, X., Ding, L., Zeng, X., Xu, J., & Peng, C. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.342519 · Cited by 11

High-throughput tagging of endogenous loci for rapid characterization of protein function

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CRISPR/Cas-based devices for mammalian synthetic biology

Kim, T., & Lu, T. K. (2019) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2019.04.015 · Cited by 11

Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant.

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Critical cancer vulnerabilities identified by unbiased CRISPR/Cas9 screens inform on efficient cancer Immunotherapy

Potts, M. A., McDonald, J. A., Sutherland, K. D., & Herold, M. J. (2020) · European Journal of Immunology · doi:10.1002/eji.202048712 · Cited by 11

Deciphering the role of Enterococcus faecium cytidine deaminase in gemcitabine resistance of gallbladder cancer

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Precise Gene Knock‐In Tools with Minimized Risk of DSBs: A Trend for Gene Manipulation

Liu, Y. H., Kong, J., Liu, G., Li, Z., & Xiao, Y. (2024) · Advanced Science · doi:10.1002/advs.202401797 · Cited by 10

Comprehensive evaluation and prediction of editing outcomes for near-PAMless adenine and cytosine base editors

Zhou, X., Gao, J., Luo, L., Huang, C., Wu, J., & Wang, X. (2024) · Communications Biology · doi:10.1038/s42003-024-07078-5 · Cited by 11

DNA nanotechnology-based strategies for minimising hybridisation-dependent off-target effects in oligonucleotide therapies

Li, X., Hu, H., Wang, H., Liu, J., Jiang, W., Zhou, F., & Zhang, J. (2024) · Materials Horizons · doi:10.1039/d4mh01158a · Cited by 12

Plant base editing: a decade of progress and future applications

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DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage

Wu, X., Lam, W. H., Zhao, Z., Cao, Y., Lin, H., Feng, X., Zhai, Y., & Hsing, I. (2026) · Nature Biotechnology · doi:10.1038/s41587-026-03120-5 · Cited by 12

Patents and technology transfer in CRISPR technology

Grobler, L., Suleman, E., & Raj, D. B. T. G. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.009 · Cited by 12

Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation

Edmondson, C., Zhou, Q., & Liu, X. (2021) · BMC Biotechnology · doi:10.1186/s12896-021-00707-5 · Cited by 10

dCas9 techniques for transcriptional repression in mammalian cells: Progress, applications and challenges

Li, Y., & Zhou, L. (2021) · BioEssays · doi:10.1002/bies.202100086 · Cited by 11

Expanding the target range of base editing in plants without loss of efficiency by blocking RNA‐silencing

Mao, Y., Wang, M., Zhao, Y., Huang, B., Wu, Q., Zheng, Q., Botella, J. R., & Zhu, J. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13712 · Cited by 10

Phenotypic Characterization of High Carotenoid Tomato Mutants Generated by the Target-AID Base-Editing Technology

Hunziker, J., Nishida, K., Kondo, A., Ariizumi, T., & Ezura, H. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.848560 · Cited by 10

Applying CRISPR-Cas9 screens to dissect hematological malignancies

Iyer, D. N., Schimmer, A. D., & Chang, H. (2022) · Blood Advances · doi:10.1182/bloodadvances.2022008966 · Cited by 10

Adenine base editor‐based correction of the cardiac pathogenic Lmna c. 1621C > T mutation in murine hearts

Yang, L., Liu, Z., Sun, J., Chen, Z., Gao, F., & Guo, Y. (2024) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.18145 · Cited by 10

More precise, more universal and more specific – the next generation of RNA‐guided endonucleases for genome editing

Marzec, M., & Hensel, G. (2019) · FEBS Journal · doi:10.1111/febs.15079 · Cited by 10

Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

Thompson, M. K., Sobol, R. W., & Prakash, A. (2021) · Biology · doi:10.3390/biology10060530 · Cited by 10

Expanding the range of CRISPR/Cas9-directed genome editing in soybean

He, R., Zhang, P., Yan, Y., Chen, Y., Jiang, L., Zhu, Y., & Wang, D. (2021) · aBIOTECH · doi:10.1007/s42994-021-00051-4 · Cited by 11

Rare immune diseases paving the road for genome editing-based precision medicine

Pavel-Dinu, M., Borna, Š., & Bacchetta, R. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1114996 · Cited by 10

Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7)

Liu, Z., Chen, M., Shan, H., Chen, S., Xu, Y., Song, Y., Zhang, Q., Yuan, H., Ouyang, H., Li, Z., et al. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03110-8 · Cited by 10

Targeted gene disruption by CRISPR/xCas9 system in Drosophila melanogaster

Ni, X., Zhou, Z., Huang, J., & Qiao, X. (2020) · Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.21662 · Cited by 10

A highly efficient gene disruption strategy reveals lipid co-regulatory networksPreprint

原山, 武., Hashidate‐Yoshida, T., Fleuriot, L., Aguilera-Romero, A., Hamano, F., Ri, K., Morimoto, R., Debayle, D., Shimizu, T., & Riezman, H. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.11.24.395632 · Cited by 11

Expanding the flexibility of base editing for high-throughput genetic screens in bacteria

Gawlitt, S., Collins, S. P., Yu, Y., Blackman, S. A., Barquist, L., & Beisel, C. L. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae174 · Cited by 9

High-Throughput Screening of PAM-Flexible Cas9 Variants for Expanded Genome Editing in the Silkworm (Bombyx mori)

Sun, L., Zhang, T., Lan, X., Zhang, N., Wang, R., Ma, S., Zhao, P., & Xia, Q. (2024) · Insects · doi:10.3390/insects15040241 · Cited by 9

PAMless SpRY exhibits a preference for the seed region for efficient targeting

Yang, C., Zhou, Z., Sun, X., Ju, H., Yue, X., Rao, S., & Xue, C. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114225 · Cited by 9

Systematic pegRNA design with PRIDICT2.0 and ePRIDICT for efficient prime editing

Mathis, N., Marquart, K. F., Allam, A., Krauthammer, M. O., & Schwank, G. (2025) · Nature Protocols · doi:10.1038/s41596-025-01244-7 · Cited by 9

Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae

Teng, X., Wang, Z., Zhang, Y., Wang, B., Gong, G., Hu, J., Zhu, Y., Peng, B., Wang, J., Chen, J. L., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-62886-5 · Cited by 12

Activity-based selection for enhanced base editor mutational scanning

Kaplan, E. L., Steger, R. J., Shah, S. T., Drepanos, L. M., Griffith, A. L., Reint, G., & Doench, J. G. (2025) · Nature Genetics · doi:10.1038/s41588-025-02366-0 · Cited by 9

In vivo base editing rescues ADPKD in a humanized mouse model

Cheng, A. S., Li, L. X., Zhou, J. X., Harris, P. C., Calvet, J. P., & Li, X. (2025) · Nature Communications · doi:10.1038/s41467-025-65997-1 · Cited by 9

CRISPR/Cas gene editing for plant resistance to pests, diseases, and herbicide: application, risk assessment, and safety evaluation

Song, X., Dang, C., Wang, F., Yao, H., Song, Q., & Yè, G. (2026) · GM crops & food · doi:10.1080/21645698.2026.2641893 · Cited by 9

Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility

Zhang, C., Kang, G., Liu, X., Zhao, S., Yuan, S., Li, L., Yang, Y., Wang, F., Zhang, X., & Yang, J. (2020) · Frontiers in Genome Editing · doi:10.3389/fgeed.2020.618385 · Cited by 10

CRISPR-Cas systems for genome editing of mammalian cells

Mani, I., Arazoe, T., & Singh, V. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.011 · Cited by 9

CRISPR/Cas: The New Frontier in Plant Improvement

Maximiano, M. R., & Franco, O. L. (2022) · ACS Agricultural Science & Technology · doi:10.1021/acsagscitech.1c00279 · Cited by 9

Discovery in CRISPR-Cas9 system.

Pan, S., & Zhang, H. (2021) · PubMed Central · doi:10.11817/j.issn.1672-7347.2021.210169 · Cited by 9

Advances in CRISPR/Cas technologies and their application in plants

Chao, D., Xi, Y., Satheesh, V., & Lei, M. (2023) · Tropical Plants · doi:10.48130/tp-2023-0002 · Cited by 9

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Zheng, S., Liang, F., Zhang, Y., Fei, J., Qin, W., & Liu, Y. (2023) · Journal of Visualized Experiments · doi:10.3791/64977 · Cited by 9

Developing a PAM-Flexible CRISPR-Mediated Dual-Deaminase Base Editor to Regulate Extracellular Electron Transport in Shewanella oneidensis

Wang, T., Zhang, J., Liang, W., Zhao, D., Bi, C., Liu, Q., Xu, N., & Liu, J. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00045 · Cited by 9

CRISPR-based precision medicine for hematologic disorders: Advancements, challenges, and prospects

Sahu, S., Poplawska, M., Lim, S. H., & Dutta, D. (2023) · Life Sciences · doi:10.1016/j.lfs.2023.122165 · Cited by 9

Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope

Zhao, D., Gao, X., Zhou, J., Li, J., Qian, Y., Wang, D., Niu, W., Zhang, T., Hu, M., Xiong, H., et al. (2023) · BMC Biology · doi:10.1186/s12915-023-01754-4 · Cited by 9

Deconstructing cancer with precision genome editing

Johnson, G. A., Gould, S. I., & Rivera, F. J. S. (2024) · Biochemical Society Transactions · doi:10.1042/bst20230984 · Cited by 11

AlPaCas: allele-specific CRISPR gene editing through a protospacer-adjacent-motif (PAM) approach

Rosignoli, S., Lustrino, E., Conci, A., Fabrizi, A., Rinaldo, S., Latella, M. C., Enzo, E., Prosseda, G., Rosa, L. D., Luca, M. D., et al. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae419 · Cited by 9

Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins

Cai, Z., Xie, W., & Bao, Z. (2024) · Trends in Microbiology · doi:10.1016/j.tim.2024.05.004 · Cited by 12

Upregulated microRNA‑330‑3p promotes calcification in the bicuspid aortic valve via targeting CREBBP

Zheng, R., Liu, H., Gu, J., Ni, B., Sun, H., Guo, Y., Chen, S., He, K., Du, J., & Shao, Y. (2020) · Molecular Medicine Reports · doi:10.3892/mmr.2020.11297 · Cited by 9

Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference

Kim, B.-J., Kim, H. J., & Lee, S. J. (2020) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2008.08058 · Cited by 9

Current technological interventions and applications of CRISPR/Cas for crop improvement

Shah, P., Magar, N. D., & Barbadikar, K. M. (2021) · Molecular Biology Reports · doi:10.1007/s11033-021-06926-5 · Cited by 9

CRISPR-Cas Assisted Shotgun Mutagenesis Method for Evolutionary Genome Engineering

Zhao, M., Gao, M., Xiong, L., Liu, Y., Tao, X., Gao, B., Liu, M., Wang, F., & Wei, D. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00112 · Cited by 9

Mammalian synthetic biology by CRISPRs engineering and applications

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Convergence of human pluripotent stem cell, organoid, and genome editing technologies

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Functional Characterization and Improved Engineering of the PAM-flexible Cas9 Variant, SpG-Cas9

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