Engineered CRISPR-Cas9 nuclease with expanded targeting space

Coot : model-building tools for molecular graphics

Emsley, P., & Cowtan, K. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904019158 · Cited by 30,571

PHENIX : a comprehensive Python-based system for macromolecular structure solution

Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

XDS

Kabsch, W. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909047337 · Cited by 16,463

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

DNA targeting specificity of RNA-guided Cas9 nucleases

Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741

How good are my data and what is the resolution?

Evans, P. R., & Murshudov, G. N. (2013) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444913000061 · Cited by 4,713

Molecular replacement with MOLREP

Vagin, A. A., & Teplyakov, A. (2009) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909042589 · Cited by 3,580

Expanding PAM recognition and enhancing base editing activity of Cas9 variants with non‐PI domain mutations derived from xCas9

Xie, L., Hu, Y. F., Li, L., Jiang, L., Jiao, Y., Wang, Y., Zhou, L., Tao, R. X., Qu, J., Chen, Q., et al. (2022) · FEBS Journal · doi:10.1111/febs.16457 · Cited by 9

In Vitro Production of Bioactive Compounds from Plant Cell Culture

Lakshmaiah, V. V., Banadka, A., Thirumoorthy, G., Naik, P. M., Al-Khayri, J. M., & Nagella, P. (2022) · Springer eBooks · doi:10.1007/978-981-16-8858-4_2 · Cited by 8

Application of Nicotinamide to Culture Medium Improves the Efficiency of Genome Editing in Hexaploid Wheat

Wang, W., Huang, P., Dai, W., Tang, H., Qiu, Y., Chang, Y., Han, Z., Li, X., Du, L., Ye, X., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24054416 · Cited by 8

Advancements in pre-clinical development of gene editing-based therapies to treat inherited retinal diseases

Chirco, K. R., Martinez, C., & Lamba, D. A. (2023) · Vision Research · doi:10.1016/j.visres.2023.108257 · Cited by 8

Engineering a Streptococcus Cas9 Ortholog with an RxQ PAM-Binding Motif for PAM-Free Gene Control in Bacteria

Teng, Y., Wang, J., Jiang, T., Zou, Y., & Yan, Y. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00366 · Cited by 8

A Novel Eukaryote‐Like CRISPR Activation Tool in Bacteria: Features and Capabilities

Liu, Y., & Wang, B. (2020) · BioEssays · doi:10.1002/bies.201900252 · Cited by 8

Prime editing enables precise genome editing in mouse liver and retinaPreprint

Jang, H., Shin, J. H., Jo, D. H., Seo, J. H., Yu, G., Gopalappa, R., Cho, S., Kim, J. H., & Kim, S. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.01.08.425835 · Cited by 8

Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome editing

Jeong, S. H., Lee, H. J., & Lee, S. J. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2024.1471720 · Cited by 7

Functionalized chitosan as nano-delivery platform for CRISPR-Cas9 in cancer treatment

Nawaz, A., Ariffin, N. S., & Wong, T. W. (2025) · Asian Journal of Pharmaceutical Sciences · doi:10.1016/j.ajps.2025.101041 · Cited by 7

The path to biotechnological singularity: Current breakthroughs and outlook

Wen, Z., Yang, D., Yang, Y., Hu, J., Parviainen, A., Chen, X., Li, Q., VanDeusen, E. A., Ma, J., & Tay, F. R. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108667 · Cited by 7

CRISPR-Cas technologies: Emerging tools from research to clinical application

Hyeon, H., Hwang, S., Luo, Y., Shin, E., Yeom, J., Kim, H., Ryu, M., & Lee, K. (2025) · The Journal of Microbiology · doi:10.71150/jm.2504012 · Cited by 7

ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient

Krausz, S. L., Simon, D. A., Bartos, Z., Biczók, Z., Varga, É., Huszár, K., Kulcsár, P. I., Tálas, A., Ligeti, Z., & Welker, E. (2025) · Nature Catalysis · doi:10.1038/s41929-025-01406-6 · Cited by 7

A long journey towards genome editing technologies in plants: a technical and critical review of genome editing technologies

Gallo, D., Meunier, A., & Périn, C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1663352 · Cited by 10

Therapeutic gene editing is here, can regulations keep up?

Kannan, S., & Najjar, D. (2020) · MIT Science Policy Review · doi:10.38105/spr.czm9c2w8ig · Cited by 7

Gene Editing in Dimorphic Fungi Using CRISPR/Cas9

Kujoth, G. C., Sullivan, T. D., & Klein, B. S. (2020) · Current Protocols in Microbiology · doi:10.1002/cpmc.132 · Cited by 8

Genome editing in plants

Wada, N., Osakabe, K., & Osakabe, Y. (2022) · Gene and Genome Editing · doi:10.1016/j.ggedit.2022.100020 · Cited by 7

Generation of Endogenous Promoter-Driven Luciferase Reporter System Using CRISPR/Cas9 for Investigating Transcriptional Regulation of the Core Clock Gene BMAL1

Sun, C., Li, C., Liu, W., & Schiöth, H. B. (2022) · Biomedicines · doi:10.3390/biomedicines10123108 · Cited by 8

When push comes to shove - RNA polymerase and DNA-bound protein roadblocks

Hao, N., Donnelly, A. J., Dodd, I. B., & Shearwin, K. E. (2023) · Biophysical Reviews · doi:10.1007/s12551-023-01064-7 · Cited by 7

CRISPR/Cas9 improves targeted knock-in efficiency in Aspergillus oryzae

Todokoro, T., Hata, Y., & Ishida, H. (2024) · Biotechnology Notes · doi:10.1016/j.biotno.2024.03.002 · Cited by 7

CRISPR technology in human diseases

Feng, Q., Li, Q., Zhou, H., Wang, Z., Lin, C., Jiang, Z., Liu, T., & Wang, D. (2024) · MedComm · doi:10.1002/mco2.672 · Cited by 8

Efficient Rescue of Retinal Degeneration in Pde6a Mice by Engineered Base Editing and Prime Editing

Liu, Z., Chen, S., Davis, A. E., Lo, C., Wang, Q. K., Li, T., Ning, K., Zhāng, Q., Zhao, J., Wang, S., et al. (2024) · Advanced Science · doi:10.1002/advs.202405628 · Cited by 7

Research on CRISPR/system in major cancers and its potential in cancer treatments

Liu, Z., Liao, Z., Chen, Y., Zhou, L., Huangting, W., & Xiao, H. (2020) · Clinical & Translational Oncology · doi:10.1007/s12094-020-02450-3 · Cited by 7

The use of base editing technology to characterize single nucleotide variants

McDaniel, S., Komor, A. C., & Goren, A. (2022) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2022.03.031 · Cited by 7

nCas9 Engineering for Improved Target Interaction Presents an Effective Strategy to Enhance Base Editing

Zhang, G., Song, Z., Huang, S., Wang, Y., Sun, J., Qiao, L., Li, G., Feng, Y., Han, W., Tang, J., et al. (2024) · Advanced Science · doi:10.1002/advs.202405426 · Cited by 7

Evolution at the Cutting Edge: CRISPR-Mediated Directed Evolution

Abbott, T. R., & Qi, L. S. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.10.027 · Cited by 7

CRISPR/Cas9 Editing: Sparking Discussion on Safety in Light of the Need for New Therapeutics

Carlaw, T., Zhang, L., & Ross, C. J. (2020) · Human Gene Therapy · doi:10.1089/hum.2020.111 · Cited by 7

Modulating Cas9 activity for precision gene editing

Uslu, M., Si̇yah, P., Harvey, A. J., & Kocabaş, F. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.015 · Cited by 7

Harnessing the power of directed evolution to improve genome editing systems

Su, Q., Zhou, M., Cheng, C., & Niu, J. (2021) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2021.02.004 · Cited by 8

Lb2Cas12a and its engineered variants mediate genome editing in human cells

Liu, X., Li, L., Tang, L., Xie, H., Gu, L., Lv, X., Liu, C., Zhao, J., Deng, R., Liu, Y., et al. (2021) · The FASEB Journal · doi:10.1096/fj.202001013rr · Cited by 7

Accurate Detection of Rare Mutant Alleles by Target Base-Specific Cleavage with the CRISPR/Cas9 System

Lee, D., Lee, J. H., & Bang, D. (2021) · ACS Synthetic Biology · doi:10.1021/acssynbio.1c00056 · Cited by 7

Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9

Hänggeli, K. P. A., Hemphill, A. F., Müller, N., Schimanski, B., Olias, P., Müller, J., & Boubaker, G. (2022) · PLoS ONE · doi:10.1371/journal.pone.0271011 · Cited by 7

Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing

Carrozzo, I., Maule, G., Gentile, C., Umbach, A., Ciciani, M., Guidone, D., Santis, M. D., Petris, G., Galietta, L. J. V., Arosio, D., et al. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.01.011 · Cited by 6

Genome editing with programmable base editors in human cells

Osgood, N. R. B., Zawalick, N. M., Sawyer, C. B., Cowan, Q. T., Gu, S., Mawson, S. J., Ranzau, B. L., Li, L., Gymrek, M., Goren, A., et al. (2025) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2025.01.001 · Cited by 6

Recent Advances in CRISPR/Cas9 Technology for Engineering Disease Resistance in Plants

Bhalerao, S., Okoń, S., Rai, M., Jadhav, P., & Prokisch, J. (2025) · Acta Agrobotanica · doi:10.5586/aa/201389 · Cited by 6

Guarding the genome: strategies for off-target detection and minimization in CRISPR/Cas9 system

Lotfi, M., Yazdi, Z. F., Hashemi, M. R., Abbaszadegan, M. R., & Sharif, S. (2025) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2025.2581677 · Cited by 6

A primer on prime: A prime editing update from advances to first-in-human trial

Lushington, C., Thomas, P. Q., & Adikusuma, F. (2026) · Molecular Therapy · doi:10.1016/j.ymthe.2026.04.033 · Cited by 6

Treatment of Huntington’s disease with a pan-HTT-targeting CRISPR nuclease

Tan, K., Siller, D. D. B., Xiong, A. Y., Wang, A. X. A., McCallister, T. X., Mummadi, S., John, L. A. S., Lee, T. K., Carrillo, A., Renshaw, D. G., et al. (2026) · Molecular Therapy · doi:10.1016/j.ymthe.2026.04.038 · Cited by 6

Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM

Liu, Y., Liang, F., Dong, Z., Li, S., Ye, J., & Qin, W. (2021) · Cells · doi:10.3390/cells10082099 · Cited by 7

Whole-Genome Sequencing Reveals Rare Off-Target Mutations in MC1R -Edited Pigs Generated by Using CRISPR-Cas9 and Somatic Cell Nuclear Transfer

Li, Z., Lan, J., Shi, X., Lu, T., Hu, X., Liu, X., Chen, Y., & He, Z. (2024) · The CRISPR Journal · doi:10.1089/crispr.2023.0034 · Cited by 6

Navigating the prime editing strategy to treat cardiovascular genetic disorders in transforming heart health

Hosseini, S. Y., Mallick, R., Mäkinen, P., & Ylä‐Herttuala, S. (2024) · Expert Review of Cardiovascular Therapy · doi:10.1080/14779072.2024.2328642 · Cited by 6

Deciphering the Role of CRISPR/Cas9 in the Amelioration of Abiotic and Biotic Stress Conditions

Singh, S., Chaudhary, R., Chaturvedi, S., & Tiwari, S. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_8 · Cited by 6

SpRY-mediated screens facilitate functional dissection of non-coding sequences at single-base resolution

Yao, Y., Zhou, Z., Wang, X., Liu, Z., Zhai, Y., Chi, X., Du, J., Luo, L., Zhao, Z., Wang, X., et al. (2024) · Cell Genomics · doi:10.1016/j.xgen.2024.100583 · Cited by 6

Modeling and correction of protein conformational disease in iPSC-derived neurons through personalized base editing

Konishi, C. T., Mulaiese, N., Butola, T., Zhang, Q., Kagan, D., Yang, Q., Pressler, M., Dirvin, B. G., Devinsky, O., Basu, J., et al. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102441 · Cited by 6

Generating novel plant genetic variation via genome editing to escape the breeding lottery

Schleif, N., Kaeppler, S. M., & Kaeppler, H. F. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10213-0 · Cited by 6

Origin of the genome editing systems: application for crop improvement

Viviani, A., Spada, M., Giordani, T., Fambrini, M., & Pugliesi, C. (2022) · Biologia · doi:10.1007/s11756-022-01142-3 · Cited by 6

Unbiased prediction of off‐target sites in genome‐edited rice using SITE‐Seq analysis on a web‐based platform

Narushima, J., Kimata, S., Shiwa, Y., Gondo, T., Akimoto, S., Soga, K., Yoshiba, S., Nakamura, K., Shibata, N., & Kondo, K. (2022) · Genes to Cells · doi:10.1111/gtc.12985 · Cited by 7

Enabling Precision Medicine with CRISPR-Cas Genome Editing Technology: A Translational Perspective

Ilahibaks, N. F., Hulsbos, M. J., Lei, Z., Vader, P., & Sluijter, J. P. G. (2022) · Advances in experimental medicine and biology · doi:10.1007/978-981-19-5642-3_20 · Cited by 7

Prime Editing in Mammals: The Next Generation of Precision Genome Editing

Wang, D., Fan, X., Li, M., Liu, T., Lü, P., Wang, G., Li, Y., Han, J., & Zhao, J. (2022) · The CRISPR Journal · doi:10.1089/crispr.2022.0084 · Cited by 6

Induced Pluripotent Stem Cells and Genome-Editing Tools in Determining Gene Function and Therapy for Inherited Retinal Disorders

Benati, D., Leung, A., Perdigão, P. R. L., Toulis, V., van der Spuy, J., & Recchia, A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232315276 · Cited by 7

Computational analysis of cas proteins unlocks new potential in HIV-1 targeted gene therapy

Dampier, W., Berman, R. E., Nonnemacher, M. R., & Wigdahl, B. L. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1248982 · Cited by 6

A zero-background CRISPR binary vector system for construction of sgRNA libraries in plant functional genomics applications

Yun, J., Kim, S., Kim, S., & Kim, J. (2019) · Plant Biotechnology Reports · doi:10.1007/s11816-019-00567-8 · Cited by 6

Base editing in pigs for precision breeding

Song, Y., Yu, W. A. N. G., Yanfang, W. A. N. G., & Jianguo, Z. H. A. O. (2020) · Frontiers of Agricultural Science and Engineering · doi:10.15302/j-fase-2019308 · Cited by 6

Expansion of CRISPR Targeting Sites Using an Integrated Gene-Editing System in Apis mellifera

Liang, L., Li, Z., Li, Q., Wang, X., Su, S., & Nie, H. (2021) · Insects · doi:10.3390/insects12100954 · Cited by 6

Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies

Lee, K. Z., Mechikoff, M. A., Parasa, M. K., Rankin, T. J., Pandolfi, P., Fitzgerald, K., Hillman, E. T., & Solomon, K. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.1c00340 · Cited by 6

Versatile and efficient genome editing with Neisseria cinerea Cas9

Liu, Z., Chen, S., Xie, W., Yu, H., Lai, L., & Li, Z. (2022) · Communications Biology · doi:10.1038/s42003-022-04258-z · Cited by 7

Reduced off-target effect of NG-BE4max by using NG-HiFi system

Shan, H., Liu, Z., Jia, Y., Chen, S., Chen, M., Song, Y., Sui, T., Lai, L., & Li, Z. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.05.012 · Cited by 6

A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutationsPreprint

Sakata, R. C., Ishiguro, S., Mori, H., Tanaka, M., Seki, M., Masuyama, N., Nishida, K., Nishimasu, H., Kondo, A., Nureki, O., et al. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/729269 · Cited by 6

Targeted genome editing in grape using multiple CRISPR-guided editing systemsPreprint

Ren, C., Lin, Y., Li, H., Li, S., & Liang, Z. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.08.22.504768 · Cited by 6

The Advancement of Prime Editing Technology

Zhang, L., Zhao, D., Wei, Z., Zhu, X. G., Sha, T., Tang, W., Bi, C., & Zhang, X. (2025) · ChemBioChem · doi:10.1002/cbic.202500193 · Cited by 5

Gene Therapy for Inherited Retinal Disease: Current Strategies, Personalized Medicine, and Future Implications—A Comprehensive Review

Butt, F. R., Dhivagaran, T., Li, B., Ashamalla, M., Tao, B. K.-L., Balas, M., Pereira, A., Yan, P., & Arjmand, P. (2025) · Journal of Personalized Medicine · doi:10.3390/jpm15120619 · Cited by 6

A Minimal and Portable CRISPR Platform Based on Bifidobacterial Cas9 Enables Genome Editing in E. coli Nissle 1917

Liu, Q., Huang, Y., Zhou, R., Ding, H., Nie, Q., Gong, X., Zuo, T., Wang, S., Liang, Y., & Li, Y. (2026) · ACS Synthetic Biology · doi:10.1021/acssynbio.5c00911 · Cited by 5

Application of novel CRISPR tools in brain therapy

Shang, J., Song, F., Zhang, Z., Chen, D., & Yang, S. (2024) · Life Sciences · doi:10.1016/j.lfs.2024.122855 · Cited by 5

Gene Editing: Paving the Way for Enhancing Plant Tolerance to Abiotic Stresses-Mechanisms, Breakthroughs, and Future Prospects

Gupta, D., Saini, A., van der Vyver, C., & Panda, S. K. (2024) · Journal of Plant Growth Regulation · doi:10.1007/s00344-024-11395-8 · Cited by 5

Advancing CRISPR base editing technology through innovative strategies and ideas

Fan, X., Lei, Y., Wang, L., Wu, X., & Li, D. (2024) · Science China Life Sciences · doi:10.1007/s11427-024-2699-5 · Cited by 5

The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants

Tan, W. Z., Wang, Z., & Liu, L. (2024) · Agriculture · doi:10.3390/agriculture15010029 · Cited by 5

General and robust sample preparation strategies for cryo-EM studies of CRISPR-Cas9 and Cas12 enzymes

Omura, S. N., & Nureki, O. (2025) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2025.01.052 · Cited by 5

The applications of CRISPR/Cas-mediated genome editing in genetic hearing loss

Wu, J., Tao, Y., Deng, D., Meng, Z., & Zhao, Y. (2023) · Cell & Bioscience · doi:10.1186/s13578-023-01021-7 · Cited by 5

Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations

Seys, F. M., Humphreys, C. M., Tomi-Andrino, C., Li, Q., Millat, T., Yang, S., & Minton, N. P. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1211197 · Cited by 5

Protein engineering technologies for development of next-generation genome editors

Winter, J., Shirguppe, S., & Pérez‐Piñera, P. (2023) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2023.100514 · Cited by 5

The Development and Application of a Base Editor in Biomedicine

Wang, F., Zeng, Y., Wang, Y., & Niu, Y. (2020) · BioMed Research International · doi:10.1155/2020/2907623 · Cited by 5

Advances in base editing with an emphasis on an AAV-based strategy

Kuang, J., Lyu, Q., Wang, J., Cui, Y., & Zhao, J. (2021) · Methods · doi:10.1016/j.ymeth.2021.03.015 · Cited by 5

Repurposing CRISPR-Cas Systems as Genetic Tools for the Enterobacteriales

Backes, N., & Phillips, G. J. (2021) · EcoSal Plus · doi:10.1128/ecosalplus.esp-0006-2020 · Cited by 5

Two Compact Cas9 Ortholog-Based Cytosine Base Editors Expand the DNA Targeting Scope and Applications In Vitro and In Vivo

Wu, S., Li, L., Li, M., Sun, S., Zhao, Y., Xue, X., Chen, F., Zhong, J., Guo, J., Qu, Q., et al. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.809922 · Cited by 5

CRISPR-Cas Based Precision Breeding in Date Palm: Future Applications

Sattar, M. N., Iqbal, Z., & Al-Khayri, J. M. (2021) · Compendium of plant genomes · doi:10.1007/978-3-030-73750-4_9 · Cited by 5

Utilization of CRISPR-Mediated Tools for Studying Functional Genomics in Hematological Malignancies: An Overview on the Current Perspectives, Challenges, and Clinical Implications

Solayappan, M., Azlan, A., Khor, K. Z., Yik, M. Y., Khan, M., Yusoff, N. M., & Moses, E. J. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2021.767298 · Cited by 5

Dead Cas Systems: Types, Principles, and Applications

Brezgin, S., Kostyusheva, A., Kostyushev, D. S., & Chulanov, V. (2020) · Vide Leaf, Hyderabad eBooks · doi:10.37247/pamb.1.2020.14 · Cited by 5

Functional dissection of human mitotic genes using CRISPR–Cas9 tiling screensPreprint

Herman, J., Carter, D. L., Arora, S., Zhu, J., Biggins, S., & Paddison, P. J. (2022) · Genes & Development · doi:10.1101/gad.349319.121 · Cited by 5

Genome editing

McGrail, M. A., Sakuma, T., & Bleris, L. G. (2022) · Scientific Reports · doi:10.1038/s41598-022-24850-x · Cited by 5

Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and FunctionPreprint

Lane, D. D., Gottimukkala, K. S. V., Cunningham, R. A., Jwa, Y., Cassidy, M. E., Castelli, J. M. P., & Adair, J. E. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.04.02.586657 · Cited by 5

Prime editing using paired pegRNAs targeting NG- or NGG-PAM in rice

Nishizawa‐Yokoi, A., Iida, K., Mori, A., & Toki, S. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1550308 · Cited by 4

Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiS

Takahashi, G., & Miyaoka, Y. (2023) · STAR Protocols · doi:10.1016/j.xpro.2023.102364 · Cited by 4

Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes

Yuan, Y., Liao, X., Li, S., Xing, X., & Zhang, C. (2024) · Science China Life Sciences · doi:10.1007/s11427-023-2468-y · Cited by 4

Energetics of Expanded PAM Readability by Engineered Cas9-NG

Bhattacharya, S., & Satpati, P. (2025) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.5c00011 · Cited by 4

Directed Evolution of OgeuIscB With Enhanced Activity in Human Cells

Lv, J., Jin, J., Ding, L., Xiang, L., Xie, B., Wu, K., & Chen, Q. (2025) · The FASEB Journal · doi:10.1096/fj.202500082r · Cited by 4

Optimizing gene editing in pigs: The role of electroporation and lipofection

Piñeiro-Silva, C., & Gadea, J. (2025) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2025.107874 · Cited by 4

Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean

Jeong, Y. Y., Han, J. H., Yu, J., Bae, S., & Seo, P. J. (2025) · BMB Reports · doi:10.5483/bmbrep.2024-0102 · Cited by 4

Visualization of a multi-turnover Cas9 after product release

Kiernan, K. A., & Taylor, D. W. (2025) · Nature Communications · doi:10.1038/s41467-025-60668-7 · Cited by 4

Off-target interactions in the CRISPR-Cas9 Machinery: mechanisms and outcomes

Kanazhevskaya, L. Y., Zhdanova, P. V., Chernonosov, A. А., & Koval, V. V. (2025) · Biochemistry and Biophysics Reports · doi:10.1016/j.bbrep.2025.102134 · Cited by 5

GenomePAM directs PAM characterization and engineering of CRISPR-Cas nucleases using mammalian genome repeats

Yu, M., Ai, L., Wang, B., Lian, S., Ip, L., Liu, J., Li, L. X., Tsai, S. Q., Kleinstiver, B. P., & Zheng, Z. (2025) · Nature Biomedical Engineering · doi:10.1038/s41551-025-01464-y · Cited by 5

Coiled-coil heterodimer-mediated split base editing systems enable flexible and robust nucleotide substitutions

Mu, S., Li, Q., Chen, M., Li, Z., Ma, Y., Li, Y., Song, Y., Hou, S., Ding, Y., Ju, J., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-68469-2 · Cited by 4

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Zhang, R., Tang, X., He, Y., Wang, W., Ren, Q., Qi, Y., & Zhang, Y. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70155 · Cited by 4

Ablation of PKCα Phosphorylation by CRISPR-Cas9 Base Editing Rescues Heart Failure

Tadokoro, T., Li, H., Gan, P., Xu, Z., Tan, W. H., Alzhanov, D., Sanchez‐Ortiz, E., McAnally, J., Guo, L. M., Xu, L., et al. (2026) · Circulation Research · doi:10.1161/circresaha.125.326738 · Cited by 4

Application and Research Prospects of CRISPR/Cas Gene Editing Technology in Lactic Acid Bacteria

张二红, Yan, J., Du, J., Chu, X., & Chen, D. (2026) · Microorganisms · doi:10.3390/microorganisms14040739 · Cited by 4

CRISPR in clinical oncology: translational advances from molecular diagnostics to therapeutics

Grigg, S. E., Shembrey, C., Fareh, M., Blombery, P. A., Corn, J. E., Seymour, J. F., & Casan, J. M. L. (2026) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-026-01179-2 · Cited by 4

Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast

Ishikawa, K., & Saitoh, S. (2023) · Biomolecules · doi:10.3390/biom13040716 · Cited by 4

Base editing correction of OCRL in Lowe syndrome: ABE-mediated functional rescue in patient-derived fibroblasts

Chen, S., Lo, C., Liu, Z., Wang, Q. K., Ning, K., Li, T., & Sun, Y. (2024) · Human Molecular Genetics · doi:10.1093/hmg/ddae045 · Cited by 4

Insights into existing and futuristic treatment approach for chronic myeloid leukaemia

Tyagi, S., Singh, A. T., Sharma, N., Chaturvedi, R., & Kushwaha, H. R. (2024) · The Indian Journal of Medical Research · doi:10.25259/ijmr_1716_22 · Cited by 4

A catalog of gene editing sites and genetic variations in editing sites in model organisms

Yang, W., Zhu, J., & Jin, W. (2024) · BMC Genomics · doi:10.1186/s12864-024-11073-9 · Cited by 4

Progress of genome editing technology and developmental biology useful for radiation research

Miura, K., Ogura, A., Kobatake, K., Honda, H., & Kaminuma, O. (2020) · Journal of Radiation Research · doi:10.1093/jrr/rraa127 · Cited by 4

CRISPR-Cas Gene Perturbation and Editing in Human Induced Pluripotent Stem Cells

van Essen, M. J., Riepsaame, J., & Jacob, J. (2021) · The CRISPR Journal · doi:10.1089/crispr.2021.0063 · Cited by 4

Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM

Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 186

Expanding the targeting space of Cas9

Mao, S. (2018) · Science · doi:10.1126/science.361.6408.1211-g

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science · doi:10.1126/science.aba8853 · Cited by 1,233

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71626-2 · Cited by 2

Functional Characterization and Improved Engineering of the PAM-flexible Cas9 Variant, SpG-Cas9

Limbasia, S. (2023) · Texas Digital Library (University of Texas) · doi:10.26153/tsw/60656