Engineered CRISPR-Cas9 nuclease with expanded targeting space

Coot : model-building tools for molecular graphics

Emsley, P., & Cowtan, K. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904019158 · Cited by 30,571

PHENIX : a comprehensive Python-based system for macromolecular structure solution

Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

XDS

Kabsch, W. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909047337 · Cited by 16,463

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

DNA targeting specificity of RNA-guided Cas9 nucleases

Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741

How good are my data and what is the resolution?

Evans, P. R., & Murshudov, G. N. (2013) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444913000061 · Cited by 4,713

Molecular replacement with MOLREP

Vagin, A. A., & Teplyakov, A. (2009) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909042589 · Cited by 3,580

Precise somatic genome editing for treatment of inborn errors of immunity

Meng, Q., Sun, H., & Liu, J. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.960348 · Cited by 4

The PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity

Sapozhnikov, D. M., & Szyf, M. (2023) · Scientific Reports · doi:10.1038/s41598-023-29332-2 · Cited by 4

Genome-wide in-locus epitope tagging of Arabidopsis proteins using prime editors

Hong, C., Han, J. H., Hwang, G., Bae, S., & Seo, P. J. (2024) · BMB Reports · doi:10.5483/bmbrep.2023-0055 · Cited by 4

Molecular Basis and Genome Editing Applications of a Compact Eubacterium ventriosum CRISPR-Cas9 System

Tang, N., Wu, Z., Gao, Y., Chen, W., Wang, Z., Su, M., Ji, W., & Ji, Q. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00501 · Cited by 4

Genetic Diversity for Barley Adaptation to Stressful Environments

Fricano, A., Battaglia, R., Mica, E., Tondelli, A., Crosatti, C., Guerra, D., & Cattivelli, L. (2021) · Springer eBooks · doi:10.1007/978-3-030-75875-2_4 · Cited by 4

Safety Assessment of Transgenic Organisms in the Environment, Volume 9

OECD (2022) · Harmonisation of regulatory oversight in biotechnology · doi:10.1787/e49bd2e8-en · Cited by 4

Plant genome editing branches out

Hille, L. T., & Kleinstiver, B. P. (2021) · Nature Plants · doi:10.1038/s41477-020-00840-7 · Cited by 4

PAM-interacting domain swapping is extensively utilized in nature to evolve CRISPR-Cas9 nucleases with altered PAM specificitiesPreprint

Wang, Q., Bian, X., Du, J., Lv, Y., Tao, L., & Xie, T. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.05.01.442224 · Cited by 4

How CRISPR Is Revolutionizing the Generation of New Models for Cancer ResearchPreprint

Rivera, F. J. S., & Dow, L. E. (2023) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a041384 · Cited by 4

Enhanced Genome Editing Activity with Novel Chimeric ScCas9 Variants in Rice

Liang, Z., Wu, Y., Deng, S., Wei, S., Zhang, K., & Guo, Y. (2025) · Advanced Science · doi:10.1002/advs.202411549 · Cited by 3

RNA-DNA hybrid binding domain broadens the editing window of base editors

Yang, Y., Fu, Z., Deng, S., Wu, G., Wang, C., Luo, X., Kang, R., Chen, Y., Peng, C., Zhang, P., et al. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.06.024 · Cited by 4

Therapeutic base editing to generate a gain-of-function F9 variant for hemophilia B

Baatartsogt, N., Kashiwakura, Y., Hiramoto, T., Ito, R., Sato, R., Nagao, Y., Naruoka, H., Takata, H., Hayakawa, M., Batjargal, K., et al. (2025) · Blood · doi:10.1182/blood.2024027870 · Cited by 3

Design Rules for Expanding PAM Compatibility in CRISPR-Cas9 from the VQR, VRER and EQR variants

Vieyra, F., Pindi, C., Lisi, G. P., Morzan, U. N., & Palermo, G. (2025) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.5c06153 · Cited by 3

Chimeric antigen receptor natural killer cell therapy for solid tumors: mechanisms, clinical progress, and strategies to overcome the tumor microenvironment

Yu, X. M., Dong, J., Shao, L., & Chen, S. (2025) · Experimental Biology and Medicine · doi:10.3389/ebm.2025.10841 · Cited by 4

Viral genome editing methods and applications in the CRISPR era

Shin, K., & Kim, E. T. (2026) · Journal of Virology · doi:10.1128/jvi.02048-25 · Cited by 3

Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger

Yuan, G., Deng, S., Dai, Z., Hofstad, B. A., & Pomraning, K. (2026) · Microbial Cell Factories · doi:10.1186/s12934-026-02979-y · Cited by 4

Sequence Display Enables Large-Scale Sequence–Activity Datasets for Rapid Protein Evolution

Cheng, L.-Q., Zheng, X., Jiang, S., Hu, Y., Liu, Y.-J., Yang, K.-Q., Rui, J.-Y., Ding, H.-X., Zhang, M., Yuan, T., et al. (2026) · Nature Biotechnology · doi:10.1038/s41587-026-03087-3 · Cited by 2

Boosting genome editing of non-coding sequences in plants with glycosylase-mediated multi-nucleotide deletion editors

Liao, S., He, Y., Liu, G., Li, Y., Tang, X., Zheng, X., Qi, Y., Zhang, T., & Zhang, Y. (2026) · 中国科学通报：英文版 · doi:10.1016/j.scib.2026.05.046 · Cited by 3

Engineered ADARs enable precision A-to-G base editing of DNA

Im, H. W., Jeong, B., Lee, Y., Oh, Y. E., Jung, C., Kim, Y. W., Kim, D. Y., Lee, S., Uhm, H., Kim, Y., et al. (2026) · Nature Biotechnology · doi:10.1038/s41587-026-03223-z · Cited by 3

Mechanism of Genome Editing Tools and Their Application on Genetic Inheritance Disorders

Oh, D. H. (2024) · Global Medical Genetics · doi:10.1055/s-0044-1790558 · Cited by 3

CRISPR single base-editing: in silico predictions to variant clonal cell lines

Dickson, K.-A., Field, N. R., Blackman, T., Ma, Y., Xie, T., Kurangil, E., Idrees, S., Rathnayake, S. N. H., Mahbub, R. M., Faiz, A., et al. (2023) · Human Molecular Genetics · doi:10.1093/hmg/ddad105 · Cited by 3

Developing small Cas9 hybrids using molecular modeling

Mangin, A., Dion, V., & Menzies, G. E. (2024) · Scientific Reports · doi:10.1038/s41598-024-68107-1 · Cited by 5

Precise Base Substitution Using CRISPR/Cas-Mediated Base Editor in Rice

Endo, M., Negishi, K., & Toki, S. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-4204-7_11 · Cited by 3

KOnezumi-AID: Automation Software for Efficient Multiplex Gene Knockout Using Target-AID

Taki, T., Morimoto, K., Mizuno, S., & Kuno, A. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms252413500 · Cited by 3

Engineering of Peptide‐Inserted Base Editors with Enhanced Accuracy and Security

Chen, Q., Sun, Y., Yao, J., Lu, Y., Qiu, R., Zhou, F., Deng, Z., & Sun, Y. (2025) · Small · doi:10.1002/smll.202411583 · Cited by 3

Molecular Switch Engineering for Precise Genome Editing

Matsumoto, D., & Nomura, W. (2021) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.1c00088 · Cited by 3

Off-target effects in genome editing

Kovalchuk, I. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-85679-9.00038-6 · Cited by 3

Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes

Liu, X., Zhou, X., Li, G., Huang, S., Sun, W., Sun, Q., Li, L., Huang, X., Liu, J., & Wang, L. (2021) · The CRISPR Journal · doi:10.1089/crispr.2021.0036 · Cited by 3

Application of CRISPR-Cas-Based Genome Editing for Precision Breeding in Wheat

Wang, W., & Akhunov, E. (2022) · Springer eBooks · doi:10.1007/978-3-030-90673-3_29 · Cited by 3

Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences

Li, M., Zhao, Y., Xue, X., Zhong, J., Lin, J., Zhou, J., Yu, W., Chen, J., & Qiao, Y. (2023) · Biotechnology Journal · doi:10.1002/biot.202200533 · Cited by 3

Genome editing of patient-derived iPSCs identifies a deep intronic variant causing aberrant splicing in hemophilia A

Hiramoto, T., Inaba, H., Baatartsogt, N., Kashiwakura, Y., Hayakawa, M., Kamoshita, N., Nishimasu, H., Nureki, O., Kinai, E., & Ohmori, T. (2023) · Blood Advances · doi:10.1182/bloodadvances.2023010838 · Cited by 3

An SpG-Cas9-based cytosine base editor expands the scope of genome editing in carrot plants

Yarra, R., & Krysan, P. J. (2024) · Plant Cell Reports · doi:10.1007/s00299-024-03173-3 · Cited by 3

Engineered transcription-associated Cas9 targeting in eukaryotic cells

Goldberg, G. W., Kogenaru, M., Keegan, S., Haase, M. A. B., Kagermazova, L., Arias, M. A., Onyebeke, K., Adams, S., Beyer, D. K., Fenyö, D., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-54629-9 · Cited by 3

Enhanced genome editing with a Streptococcus equinus Cas9

Liu, J., Wang, Y., Wei, J., Wang, S., Li, M., Huang, Z., Zhang, S., Liu, H., Huang, J., & Wang, Y. (2025) · Communications Biology · doi:10.1038/s42003-025-07593-z · Cited by 3

Molecular tools for genome editing in plants: a synthetic overview

da Cunha, N. B., Leite, M. L., Rodrigues, J. C. M., Carrijo, J., Costa, F. F., Dias, S. C., Rech, E. L., & Vianna, G. R. (2025) · Vegetable Research · doi:10.48130/vegres-0025-0005 · Cited by 3

Macrobrachium rosenbergii Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors

Li, G., Zhou, X., Zhu, G., Pan, Y., Yan, J., Meng, J., Ye, T., Cheng, Y., Liu, C., & Gu, Z. (2025) · Animals · doi:10.3390/ani15152161 · Cited by 3

Targeted genome editing for the correction or alleviation of primary Immunodeficiencies

Sipe, C. J., Vázquez, P. N. C., Skeate, J. G., McIvor, R. S., & Moriarity, B. S. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.03.001 · Cited by 4

Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells

Ishiguro, S., & Yachie, N. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1441-9_12 · Cited by 3

Nanomedicine enables efficient CRISPR-Cas9 genome editing for disease treatment

Ma, L., Dong, C., Yu, M., Song, X., Yu, Y., & Chen, Y. (2021) · 中国科学通报：英文版 · doi:10.1016/j.scib.2021.11.016 · Cited by 3

Tapping into the Unsung Potential of CRISPR/CAS Technology in Agriculture

Rehman, R. S., Ali, M., Zafar, S. A., Ahmad, M., Pasha, A. N., Bashir, H., Rashid, F., & Hussain, M. (2022) · Asian Journal of Biochemistry Genetics and Molecular Biology · doi:10.9734/ajbgmb/2022/v10i430249 · Cited by 3

Can SpRY recognize any PAM in human cells?

Ye, J., Xi, H., Chen, Y., Chen, Q., Lu, X., Lv, J., Chen, Y., Gu, F., & Zhao, J. (2022) · Journal of Zhejiang University SCIENCE B · doi:10.1631/jzus.b2100710 · Cited by 3

CRISPR/Cas9: Contemporary designer nucleases for efficient genome editing in phytopathogenic fungi

Harishchandra, D. L., Chethana, K., Zhang, W., Xing, Q., Peng, J., Brooks, S., Hyde, K., Li, X., & Yan, J. (2021) · Current Research in Environmental & Applied Mycology · doi:10.5943/cream/11/1/26 · Cited by 3

Contribution of CRISPRable DNA to human complex traits

Zhai, R., Zheng, C., Yang, Z., Li, T., Chen, J., & Shen, X. (2022) · Communications Biology · doi:10.1038/s42003-022-03969-7 · Cited by 3

Genom-Editierung mit CRISPR/Cas9: Vorzeichen einer neuen Epoche der Medizin?

Schermer, B., & Benzing, T. R. (2019) · DMW - Deutsche Medizinische Wochenschrift · doi:10.1055/a-0759-7180 · Cited by 3

A Guide to Understanding “State-of-the-Art” Basic Research Techniques in Anesthesiology

Obal, D., Wu, S., McKinstry-Wu, A. R., & Tawfik, V. L. (2020) · Anesthesia & Analgesia · doi:10.1213/ane.0000000000004801 · Cited by 3

Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT)

Lee, N.-E., Kim, S. H., Yu, D., Woo, E., Kim, M.-I., Seong, G.-S., Lee, S. M., Ko, J., & Kim, Y.-S. (2020) · Communications Biology · doi:10.1038/s42003-020-01363-9 · Cited by 3

Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9Preprint

Agudelo, D., Carter, S., Velimirovic, M., Duringer, A., Levesque, S., Rivest, J.-F., Loehr, J., Mouchiroud, M., Cyr, D., Waters, P. J., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/321208 · Cited by 3

Engineered PAM-flexible FnCas9 variants for robust and specific genome editing and diagnosticsPreprint

Acharya, S., Ansari, A. H., Hirano, S., Paul, D., Rauthan, R., Kumar, M., Phutela, R., Sarkar, S., Gulati, S., Mahato, S., et al. (2021) · Research Square · doi:10.21203/rs.3.rs-990232/v2 · Cited by 3

Comprehensive Analysis of CRISPR Base Editing Outcomes for Multimeric ProteinPreprint

Kabra, M., Moosajee, M., Newby, G. A., Molugu, K., Saha, K., Liu, D. R., & Pattnaik, B. R. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.06.20.496792 · Cited by 3

High-frequency sorghum transformation toolkit enhances Cas9 efficiency and expands promoter-editing capability with SpRYPreprint

Shen, J., Aregawi, K., Anwar, S., Miller, T. I., Groover, E. D., Rajkumar, M., Savage, D. F., & Lemaux, P. G. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.01.21.634149 · Cited by 3

Adenine base editor for knockout of proteins: A practical guide from design to analysis with updated MultiEditRbatch

Eaton, E. J., Wick, B. J., Chacón, J. S., Wang, A. J., Kluesner, M., Barnes, J. T., Kar, B., Wang, M., Johnson, M. J., Skeate, J. G., et al. (2026) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2026.102908 · Cited by 2

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71626-2 · Cited by 2

Bacterial directed evolution of CRISPR base editors

Mach, R. Q., & Miller, S. M. (2025) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2025.01.003 · Cited by 2

BEstimate: a computational tool for the design and interpretation of CRISPR base editing experiments

Dinçer, C., Fussing, B., Garnett, M. J., & Coelho, M. A. (2026) · Genome biology · doi:10.1186/s13059-026-04077-z · Cited by 3

Mutational scanning reveals substrate-assisted autoregulation of the WNT destruction complex

Padmanarayana, M., Sakalas, S., Sarkar, P., Ma, M., Garvin, E. R., Lee, E. N., Corsello, S. M., Guettler, S., Pusapati, G. V., & Rohatgi, R. (2026) · Nature Genetics · doi:10.1038/s41588-026-02662-3 · Cited by 3

Loop engineering of AtCas9 for effective and broad genome editing

Zhang, Y., Huang, D.-C., Duan, M., Zhang, Y.-M., Huang, A.-H., Yin, H., & Zhang, Y. (2025) · Cell Insight · doi:10.1016/j.cellin.2025.100286 · Cited by 2

LNP‐mediated in vivo base editing corrects Agxt to cure primary hyperoxaluria type 1

Zhang, D., Zheng, R., Chen, Z., Chen, X., Yang, L., Huo, Y., Zhao, Y., Huang, J., Zhang, D., Yin, S., et al. (2025) · Clinical and Translational Medicine · doi:10.1002/ctm2.70533 · Cited by 2

CRISPR/AID-mediated multiplex genome editing in Halomonas spp.: A robust toolbox for industrial strain development

Guan, Y., Wen, R., Zhao, C., Zhang, L., Wu, F., Yang, F., & Chen, G. (2025) · Chemical Engineering Journal · doi:10.1016/j.cej.2025.171411 · Cited by 2

High-efficiency TadA cytosine base editors for precise modelling of human disease variants

Qin, W., Lin, S., Zhang, Y., Huang, K., Petree, C., Varshney, P., & Varshney, G. K. (2026) · Nature Biomedical Engineering · doi:10.1038/s41551-025-01607-1 · Cited by 2

ABE9 fused to SpRY Cas9 nickase enables precise generation of bystander free mouse models

Ong, J. K., Bhunia, S., Hilbert, B., Kirschner, V., Duglosz, S., Zimmermann, F., Freichel, M., & Cornean, A. (2026) · Scientific Reports · doi:10.1038/s41598-026-40642-z · Cited by 2

Optimizing linker length of base editors for precise crop breeding and gene therapy

Shi, Y., Yuan, Y., Qin, L., Zhou, F., Wu, G., Li, B., Yao, P., Shi, M., Ma, L., Wang, Y., et al. (2026) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2026.02.021 · Cited by 2

CRISPR Base Editing Correction of TGFBI Mutations in Autosomal Dominant Corneal Dystrophies

Chen, J., Davison, C. W., Ellis, J., Blevins, B., Presley, W., Myers, M., Kong, D., Hou, Z., Mian, S. I., Prasov, L., et al. (2026) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.67.2.60 · Cited by 2

Gene Editing Strategies for Duchenne Muscular Dystrophy: From Molecular Mechanisms to Clinical Translation

Siddika, A., Rousseau, J., Veillette, F., Bouchard, C., Lu, Y., & Tremblay, J. P. (2026) · Cells · doi:10.3390/cells15100852 · Cited by 2

From mechanism to medicine: CRISPR‒Cas9 delivery strategies, therapeutic applications and translation challenges

Makhijani, S., Alasiri, G. A., Quadri, M. S. A., & Ingle, R. G. (2026) · Discover Nano · doi:10.1186/s11671-026-04653-z · Cited by 4

Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing

Luo, X., Qu, Y., Ye, Z., Li, Z. L., Zhang, Y., Luo, L., Li, S., Wei, Z., Wang, M., Bock, R., et al. (2026) · Advanced Science · doi:10.1002/advs.76047 · Cited by 2

CRISPR–Cas systems for plant virus management: detection, surveillance, and host resistance

Kumar, S., Singh, R. M., & Gadhave, K. R. (2026) · Frontiers in Plant Science · doi:10.3389/fpls.2026.1804262 · Cited by 2

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Li, S., Li, H., Chen, B., Jiang, L., Long, K., Lang, J., Pu, X., & Xiong, E. (2026) · Interdisciplinary medicine · doi:10.1002/inmd.70162 · Cited by 2

Base editing for precision therapeutics

Regmi, M., Ma, K., Bi, C., Zhang, X., Zhao, D., Yu, L., Huihui, Y., Wang, N., & Yang, C. (2026) · Cell Genomics · doi:10.1016/j.xgen.2026.101298 · Cited by 2

Engineering DNA-Targeting CRISPR and CRISPR-Like Effectors: Advances from Rational Design and High-Throughput Screening to AI-Driven Development

She, L., Liang, Z., Zou, Q., & Huo, Y. (2026) · ACS Synthetic Biology · doi:10.1021/acssynbio.6c00386 · Cited by 2

Target residence of Cas9: challenges and opportunities in genome editing

Feng, Y.-L., Wang, M., & Xie, A. (2022) · Genome Instability & Disease · doi:10.1007/s42764-022-00066-1 · Cited by 2

CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells

Shahbazi, R., Lipson, P., Gottimukkala, K. S. V., Lane, D. D., & Adair, J. E. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2679-5_4 · Cited by 2

Design of SaCas9-HF for In Vivo Gene Therapy

Tiwari, K., Kumar, R., & Saudagar, P. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2716-7_12 · Cited by 2

Combining Off‐flow, a Nextflow‐coded program, and whole genome sequencing reveals unintended genetic variation in CRISPR/Cas-edited iPSCs

Shum, C., Han, S. Y., Thiruvahindrapuram, B., Wang, Z., de Rijke, J., Zhang, B., Sundberg, M., Chen, C.-D., Buttermore, E. D., Makhortova, N. R., et al. (2023) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2023.12.036 · Cited by 2

Genetic modification of mice using CRISPR-Cas9: Best practices and practical concepts explained

Hosur, V., Low, B. E., & Wiles, M. V. (2024) · Elsevier eBooks · doi:10.1016/b978-0-12-817218-6.00018-8 · Cited by 3

Rapid Generation Advancement for Accelerated Plant Improvement

Hamwieh, A., Abdallah, N. A., Kumar, S., Baum, M., Fouad, N., Istanbuli, T., Tawkaz, S., Kumar, T., Radwan, K. H., Maalouf, F., et al. (2024) · Sustainability Sciences in Asia and Africa · doi:10.1007/978-981-99-4673-0_5 · Cited by 2

CRISPR-Cas9 genome editing of crops: Food and nutritional security

Krishnan, R. R., Sivakumar, B., Jagannath, N., Rao, N. R., Suresh, N., Nagella, P., Al-Khayri, J. M., & Jain, S. M. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13229-2.00024-7 · Cited by 2

Genome Editing Based CRISPR System in Crop Improvement

Zamani, K., Mohsenpour, M., & Shariatpanahi, M. E. (2024) · Sustainable landscape planning and natural resources management · doi:10.1007/978-3-031-50729-8_5 · Cited by 2

Structure and engineering of Brevibacillus laterosporus Cas9

Nakane, T., Nakagawa, R., Ishiguro, S., Okazaki, S., Mori, H., Shuto, Y., Yamashita, K., Yachie, N., Nishimasu, H., & Nureki, O. (2024) · Communications Biology · doi:10.1038/s42003-024-06422-z · Cited by 3

Microcrystal Delivery Using a Syringe and Syringe Pump Method for Serial Crystallography

Nam, K. H. (2025) · Analytica—A Journal of Analytical Chemistry and Chemical Analysis · doi:10.3390/analytica6010005 · Cited by 2

Protein Engineering of DNA-Dependent Enzymes

Yudkina, A. V., & Zharkov, D. O. (2020) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-41283-8_2 · Cited by 2

Rational Selection of CRISPR-Cas Triggering Homology-Directed Repair in Human Cells

Li, F., Zhou, C., Tu, T., Liu, Y., Lv, X., Bang, W., Song, Z., Zhao, Q., Liu, C., Gu, F., et al. (2020) · Human Gene Therapy · doi:10.1089/hum.2020.247 · Cited by 2

Base editing-coupled survival screening enabled high-sensitive analysis of PAM compatibility and finding of the new possible off-target

Su, T., Guo, Q. J., Zheng, Y., Chang, Y., Gu, F., Lu, X., & Qi, Q. (2021) · iScience · doi:10.1016/j.isci.2021.102769 · Cited by 2

RiboJ-assisted non-repeated sgRNA arrays for enhanced CRISPR multiplex genome engineering in Escherichia coli

Woo, S., Kim, S. K., Kim, T. H., Kim, S., Kim, Y., Lee, S., & Lee, D. (2025) · Chemical Engineering Journal · doi:10.1016/j.cej.2025.162336 · Cited by 2

Rewired Cas9s with Minimal Sequence Constraints

Levesque, S., Agudelo, D., & Doyon, Y. (2020) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2020.04.009 · Cited by 2

Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Collins, S. P., & Beisel, C. L. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.07.030 · Cited by 2

Transversion Expansion of Base Editing

Nishida, K., & Kondo, A. (2021) · The CRISPR Journal · doi:10.1089/crispr.2021.29134.kni · Cited by 2

Genome Editing and CRISPR-Cas Technology for Enhancing Abiotic Stress Tolerance in Cereals

Chowdhury, N., Das, D., Sarki, Y. N., Sharma, M., Singha, D. L., & Chikkaputtaiah, C. (2022) · Springer eBooks · doi:10.1007/978-981-19-0140-9_11 · Cited by 2

Developing Crop Varieties by Physiological Breeding for Improving Plant Nutrition

Taria, S., Meena, S., Nagar, S., Kumar, S., & Arora, A. K. (2023) · Springer eBooks · doi:10.1007/978-981-19-7498-4_4 · Cited by 2

Base Editing

Basturea, G. N. (2019) · Materials and Methods · doi:10.13070/mm.en.9.2800 · Cited by 2

Robust Genome Editing of Single-Base PAM Targets with Engineered ScCas9 VariantsPreprint

Chatterjee, P., Jakimo, N., & Jacobson, J. M. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/620351 · Cited by 2

Preface: More than two decades of modern tumor immunology

Galluzzi, L., & Rudqvist, N.-P. (2020) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(20)30020-3 · Cited by 2

CrisPam: SNP-derived PAM analysis web tool and human pathogenic SNPs database for CRISPR allele-specific targetingPreprint

Rabinowitz, R., Darnell, R., & Offen, D. (2019) · Research Square · doi:10.21203/rs.2.9413/v1 · Cited by 2

Metabolic Engineering ofCorynebacterium glutamicum

Becker, J., & Wittmann, C. (2021) · Metabolic Engineering · doi:10.1002/9783527823468.ch12 · Cited by 2

Engineered PAM-flexible FnCas9 variants for robust and specific genome editing and diagnosticsPreprint

Acharya, S., Ansari, A. H., Hirano, S., Paul, D., Rauthan, R., Kumar, M., Phutela, R., Sarkar, S., Gulati, S., Mahato, S., et al. (2021) · Research Square · doi:10.21203/rs.3.rs-990232/v1 · Cited by 2

Frame Editors for Precise, Template-Free FrameshiftingPreprint

Nakade, S., Nakamae, K., Tang, T., Yu, D., Sakuma, T., Yamamoto, T., & Lu, T. K. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.12.05.518807 · Cited by 2

Hijacking and recording intracellular RNAs in human cells using eukaryotic reprogrammed tracrRNAsPreprint

Lee, S.-H., Park, J., Jung, C., & Bae, S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.28.620760 · Cited by 2

Molecular engineering and dynamic activation mechanism of a PAM-flexible compact CRISPR-Cas9Preprint

Nureki, O., Nakagawa, R., Omura, S. N., Kajimoto, S., Okazaki, S., Hirano, H., Yamashita, K., Nishimasu, H., Ishiguro, S., Mori, H., et al. (2024) · Research Square · doi:10.21203/rs.3.rs-4619764/v1 · Cited by 2

A comprehensive benchmark for multiple highly efficient base editors with broad targeting scopePreprint

Wang, X., Cheng, X., Li, Z., Ma, S., Zhang, H., Chen, Z., Yao, Y., Li, Z., Zhong, C., Li, Y., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.17.628899 · Cited by 2

Multiplexed in vivo base editing identifies functional gene-variant-context interactionsPreprint

Acosta, J., Johnson, G. A., Gould, S. I., Dong, K., Lendner, Y., Detrés, D., Atwa, O., Bulkens, J., Gruber, S., Contreras, M. E., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.02.23.639770 · Cited by 2

Intravitreal adenine base editing of RS1 improves vision in a preclinical mouse model of retinoschisis

Jo, D. H., Jang, H., Cho, C. S., Lee, S. J., Heo, J. H., Kim, J. A., Kim, S. J., Ryu, W., Park, C.-W., Kang, B.-C., et al. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.04.021 · Cited by 1

Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM

Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 186

Expanding the targeting space of Cas9

Mao, S. (2018) · Science · doi:10.1126/science.361.6408.1211-g

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science · doi:10.1126/science.aba8853 · Cited by 1,233

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71626-2 · Cited by 2

Functional Characterization and Improved Engineering of the PAM-flexible Cas9 Variant, SpG-Cas9

Limbasia, S. (2023) · Texas Digital Library (University of Texas) · doi:10.26153/tsw/60656