Cited by 652Visual perception and processing mechanismsNeural dynamics and brain functionPhotoreceptor and optogenetics research

Cortical layer-specific critical dynamics triggering perception

Open access
Marshel, James H., Kim, Yoon Seok, Machado, Timothy A., Quirin, Sean, Benson, Brandon, Kadmon, Jonathan, Raja, Cephra, Chibukhchyan, Adelaida +8 more·2019·Science

Abstract

Perceptual experiences may arise from neuronal activity patterns in mammalian neocortex. We probed mouse neocortex during visual discrimination using a red-shifted channelrhodopsin (ChRmine, discovered through structure-guided genome mining) alongside multiplexed multiphoton-holography (MultiSLM), achieving control of individually specified neurons spanning large cortical volumes with millisecond precision. Stimulating a critical number of stimulus-orientation-selective neurons drove widespread recruitment of functionally related neurons, a process enhanced by (but not requiring) orientation-discrimination task learning. Optogenetic targeting of orientation-selective ensembles elicited correct behavioral discrimination. Cortical layer-specific dynamics were apparent, as emergent neuronal activity asymmetrically propagated from layer 2/3 to layer 5, and smaller layer 5 ensembles were as effective as larger layer 2/3 ensembles in eliciting orientation discrimination behavior. Population dynamics emerging after optogenetic stimulation both correctly predicted behavior and resembled natural internal representations of visual stimuli at cellular resolution over volumes of cortex.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1126/science.aaw5202

Cites

81 of 81 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Optogenetics can stimulate neural tissue with high bandwidth, reaching single-cell resolution.

    “Researchers are also developing other methods to stimulate neuronal tissue with a high bandwidth, such as optogenetics with up to singlecell resolution (Marshel et al., 2019).”

    From Writing to the Mind’s Eye of the Blind (2020) · cites this paper as Marshel et al., 2019

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. [19]Hebb DO, Organization of Behavior (Wiley, 1949). 10643472

Cited by

Sorted by citations

Authors
Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K.
Affiliations
  • Stanford University (US)
  • Boulder Nonlinear Systems, Lafayette, CO 80026, USA.
  • The University of Tokyo (JP)
  • Howard Hughes Medical Institute (US)
Corresponding author
Deisseroth, Karl
Venue
Science
Published
2019-08-09
Volume
365
Issue
6453
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2956728914
Cited by
652
References
81
Citations by year
2026: 69 · 2025: 95 · 2024: 90 · 2023: 126 · 2022: 100 · 2021: 86 · 2020: 71 · 2019: 13
FWCI
39.23
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Howard Hughes Medical Institute
  • Wellcome Trust
  • Japan Society for the Promotion of Science (18H04136)
  • National Institute of Mental Health (R37MH075957)
  • National Institute on Drug Abuse (R13DA041796, R37DA035377, P50DA042012)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Visual perception and processing mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
Keywords
perception, orientation discrimination, orientation-selective neurons, layer 2/3, layer 5, neuronal ensembles, optogenetic stimulation, visual discrimination, internal representations, mouse neocortex
MeSH terms
Channelrhodopsins, Animals, Cells, Cultured, Holography, Neurons, Orientation, Photic Stimulation, Visual Perception, Neocortex, Mice, Opsins, Molecular Imaging, Aquatic Organisms, Optogenetics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review