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Improved Medium for Lactic Streptococci and Their Bacteriophages
Open accessAbstract
Incorporation of 1.9% β-disodium glycerophosphate (GP) into a complex medium resulted in improved growth by lactic streptococci at 30 C. The medium, called M17, contained: Phytone peptone, 5.0 g; polypeptone, 5.0 g; yeast extract, 2.5 g; beef extract, 5.0 g; lactose, 5.0 g; ascorbic acid, 0.5 g; GP, 19.0 g; 1.0 M MgSO 4 ·7H 2 O, 1.0 ml; and glass-distilled water, 1,000 ml. Based on absorbance readings and total counts, all strains of Streptococcus cremoris, S. diacetilactis , and S. lactis grew better in M17 medium than in a similar medium lacking GP or in lactic broth. Enhanced growth was probably due to the increased buffering capacity of the medium, since pH values below 5.70 were not reached after 24 h of growth at 30 C by S. lactis or S. cremoris strains. The medium also proved useful for isolation of bacterial mutants lacking the ability to ferment lactose; such mutants formed minute colonies on M17 agar plates, whereas wild-type cells formed colonies 3 to 4 mm in diameter. Incorporation of sterile GP into skim milk at 1.9% final concentration resulted in enhanced acid-producing activity by lactic streptococci when cells were inoculated from GP milk into skim milk not containing GP. M17 medium also proved superior to other media in demonstrating and distinguishing between lactic streptococcal bacteriophages. Plaques larger than 6 mm in diameter developed with some phage-host combinations, and turbid plaques, indicative of lysogeny, were also easily demonstrated for some systems.
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The main growth media used in the 64 reviewed studies were MRS (55 studies), M17 (40 studies), and PCA (31 studies).
“The main growth media used in the 64 reviewed studies were de Man-Rogosa-Sharpe (MRS; De Man et al., 1960) elective for lactobacilli (55 studies), M17 (Terzaghi and Sandine, 1975) elective for coccoid LAB (40 studies), and PCA (31 studies) for total bacterial count.”
From Lactic acid bacteria in cow raw milk for cheese production: Which and how many? (2023) · cites this paper as Terzaghi and Sandine, 1975
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Metadata
- Authors
- Terzaghi, B. E., & Sandine, W. E.
- Affiliations
- New Zealand Dairy Research Institute, Palmerston North, New Zealand
- Venue
- Applied Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1975-06
- Volume
- 29
- Issue
- 6
- Pages
- 807-813
- ISSN
- 0003-6919 (ISSN-L)
- PMC
- PMC187084
- OpenAlex
- W2139325759
- Cited by
- 2,339
- References
- 23
- Citations by year
- 2026: 9 · 2025: 18 · 2024: 20 · 2023: 24 · 2022: 32 · 2021: 34 · 2020: 32 · 2019: 33 · 2018: 36 · 2017: 35 · 2016: 38 · 2015: 42 · 2014: 39 · 2013: 54 · 2012: 61
- FWCI
- 22.73
- Type
- journal article
- Language
- en
- Update notices
- correction (1975-08-01)
- Open access
- diamond
- doi.org
- Locations
- Applied Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- lactic streptococci, Streptococcus cremoris, Streptococcus diacetilactis, Lactococcus lactis, culture medium optimization, low-temperature growth, buffering capacity, phage isolation, bacteriophages, lysogeny
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