Polydextrose, Lactitol, and Fructo-Oligosaccharide Fermentation by Colonic Bacteria in a Three-Stage Continuous Culture System
Open accessAbstract
In vitro fermentations were carried out by using a model of the human colon to simulate microbial activities of lower gut bacteria. Bacterial populations (and their metabolic products) were evaluated under the effects of various fermentable substrates. Carbohydrates tested were polydextrose, lactitol, and fructo-oligosaccharide (FOS). Bacterial groups of interest were evaluated by fluorescence in situ hybridization as well as by species-specific PCR to determine bifidobacterial species and percent-G+C profiling of the bacterial communities present. Short-chain fatty acids (SCFA) produced during the fermentations were also evaluated. Polydextrose had a stimulatory effect upon colonic bifidobacteria at concentrations of 1 and 2% (using a single and pooled human fecal inoculum, respectively). The bifidogenic effect was sustained throughout all three vessels of the in vitro system ( P = 0.01 seen in vessel 3), as corroborated by the bacterial community profile revealed by %G+C analysis. This substrate supported a wide variety of bifidobacteria and was the only substrate where Bifidobacterium infantis was detected. The fermentation of lactitol had a deleterious effect on both bifidobacterial and bacteroides populations ( P = 0.01) and decreased total cell numbers. SCFA production was stimulated, however, particularly butyrate (beneficial for host colonocytes). FOS also had a stimulatory effect upon bifidobacterial and lactobacilli populations that used a single inoculum ( P = 0.01 for all vessels) as well as a bifidogenic effect in vessels 2 and 3 ( P = 0.01) when a pooled inoculum was used. A decrease in bifidobacteria throughout the model was reflected in the percent-G+C profiles.
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Polydextrose acts as a substrate for beneficial endogenous microbes, increasing their levels and activity in the intestinal lumen.
“It also acts as a substrate for beneficial endogenous microbes, allowing an increase in their levels and activity in the intestinal lumen [7,8].”
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Cites
Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics
Gibson, G. R., & Roberfroid, M. B. (1995) · Journal of Nutrition · doi:10.1093/jn/125.6.1401 · Cited by 7,659 · see citing works
Self-citationAFRC, R. F. (1989) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1989.tb05105.x · Cited by 4,831 · see citing works
Probiotics in man and animals.
Fuller, R. (1989) · PubMed · Cited by 1,292 · see citing works
Manz, W., Amann, R., Ludwig, W., Vancanneyt, M., & SCHLEIFER, K. H. (1996) · Microbiology · doi:10.1099/13500872-142-5-1097 · Cited by 1,176 · see citing works
Franks, A. H., Harmsen, H. J. M., Raangs, G. C., Jansen, G. J., Schut, F., & Welling, G. W. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.9.3336-3345.1998 · Cited by 1,055 · see citing works
Langendijk-Genevaux, P. S., Schut, F., Jansen, G. J., Raangs, G. C., Kamphuis, G. R., Wilkinson, M. H. F., & Welling, G. W. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.8.3069-3075.1995 · Cited by 974 · see citing works
The Genera of Lactic Acid Bacteria
(1995) · doi:10.1007/978-1-4615-5817-0 · Cited by 685 · see citing works
Regulatory effects of bifidobacteria on the growth of other colonic bacteria
Gibson, G. R., & Wang, X. (1994) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1994.tb03443.x · Cited by 682 · see citing works
Self-citationImmunomodulation of Human Blood Cells Following the Ingestion of Lactic Acid Bacteria
Schiffrin, E. J., Rochat, F., Link‐Amster, H., Aeschlimann, J., & Donnet‐Hughes, A. (1995) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(95)76659-0 · Cited by 553 · see citing works
Macfarlane, G. T., Macfarlane, S., & Gibson, G. R. (1998) · Microbial Ecology · doi:10.1007/s002489900072 · Cited by 539 · see citing works
Self-citationMatsuki, T., Watanabe, K., Tanaka, R., Fukuda, M., & Oyaizu, H. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.10.4506-4512.1999 · Cited by 457 · see citing works
Link‐Amster, H., Rochat, F., Saudan, K.-Y., Mignot, O., & Aeschlimann, J. (1994) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.1994.tb00011.x · Cited by 432 · see citing works
Bouhnik, Y., Vahedi, K., Achour, L., Attar, A., Salfati, J., Pochart, P., Marteau, P. R., Flourié, B., Bornet, F. R. J., & Rambaud, J. (1999) · Journal of Nutrition · doi:10.1093/jn/129.1.113 · Cited by 384 · see citing works
Harmsen, P. E. H. J. M. (1999) · Microbial Ecology in Health and Disease · doi:10.1080/089106099435862 · Cited by 372 · see citing works
Holben, W. E., Williams, P. M., Saarinen, M., Särkilahti, L. K., & Apajalahti, J. (2002) · Microbial Ecology · doi:10.1007/s00248-002-1011-6 · Cited by 370 · see citing works
Self-citationEfficacy ofLactobacillus GGas a Diarrheal Preventive in Travelers
Hilton, E., Kolakowski, P., Singer, C., & Smith, M. A. M. (1997) · Journal of Travel Medicine · doi:10.1111/j.1708-8305.1997.tb00772.x · Cited by 322 · see citing works
Sgorbati, B., Biavati, B., & Palenzona, D. L. (1995) · Springer eBooks · doi:10.1007/978-1-4615-5817-0_8 · Cited by 248 · see citing works
Apajalahti, J., Särkilahti, L. K., Mäki, B. R. E., Heikkinen, J. P., Nurminen, P. H., & Holben, W. E. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.10.4084-4088.1998 · Cited by 209 · see citing works
Self-citationStudies on the effects of polydextrose intake on physiologic functions in Chinese people
Jie, Z., Bang-yao, L., Ming-jie, X., Hai-wei, L., Zu-kang, Z., Ting-song, W., & Craig, S. A. (2000) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/72.6.1503 · Cited by 207 · see citing works
Bifidogenic properties of different types of fructo-oligosaccharides
Gibson, G. R., & Wang, X. (1994) · Food Microbiology · doi:10.1006/fmic.1994.1055 · Cited by 179 · see citing works
Self-citationTuohy, K. M., Ziemer, C. J., Klinder, A., Knöbel, Y., Pool-Zobel, B. L., & Gibson, G. R. (2002) · Microbial Ecology in Health and Disease · doi:10.1080/089106002320644357 · Cited by 177 · see citing works
Self-citationThe prebiotic properties of oligofructose at low intake levels
Rao, V. A. (2001) · Nutrition Research · doi:10.1016/s0271-5317(01)00284-6 · Cited by 174 · see citing works
Fn-type Chicory Inulin Hydrolysate Has a Prebiotic Effect in Humans
Menne, E., Guggenbuhl, N., & Roberfroid, M. B. (2000) · Journal of Nutrition · doi:10.1093/jn/130.5.1197 · Cited by 164 · see citing works
Colonic Microbiota, Nutrition and Health
Gibson, G. R., & Roberfroid, M. B. (1999) · Springer eBooks · doi:10.1007/978-94-017-1079-4 · Cited by 143 · see citing works
Self-citationApajalahti, J., Kettunen, H., Kettunen, A., Holben, W. E., Nurminen, P. H., Rautonen, N., & Mutanen, M. L. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.10.4986-4995.2002 · Cited by 134 · see citing works
Self-citationLactic acid bacteria with health claims—interactions and interference with gastrointestinal flora
Mattila, T., Mättö, J., & Saarela, M. (1999) · International Dairy Journal · doi:10.1016/s0958-6946(99)00041-2 · Cited by 134 · see citing works
Butyrate inhibits colon carcinoma cell growth through two distinct pathways
Archer, S. Y., Meng, S., Wu, J. T., et al. (1998) · Surgery · doi:10.1016/s0039-6060(98)70127-8 · Cited by 121 · see citing works
DNA‐based monitoring of total bacterial community structure in environmental samples
Holben, W. E., & Harris, D. J. (1995) · Molecular Ecology · doi:10.1111/j.1365-294x.1995.tb00263.x · Cited by 119 · see citing works
Effect of Lactitol and Lactulose Administration on the Fecal Flora in Cirrhotic Patients
Riggio, O., Varriale, M., Testore, G. P., Rosa, R. D., Rosa, E. D., Merli, M., Romiti, A., Candiani, C., & Capocaccia, L. (1990) · Journal of Clinical Gastroenterology · doi:10.1097/00004836-199008000-00016 · Cited by 108 · see citing works
Caloric utilization and disposition of [14C]polydextrose in man
Figdor, S. K., & Bianchine, J. R. (1983) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00116a051 · Cited by 74 · see citing works
The Effect of Lactose Derivatives on Intestinal Lactic Acid Bacteria
Kontula, P., Suihko, M. ‐., von Wright, A., et al. (1999) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(99)75230-6 · Cited by 60 · see citing works
Assimilation of lactitol, an 'unabsorbed' disaccharide in the normal human colon.
GRIMBLE, G. K., Patil, D. H., & Silk, D. B. A. (1988) · Gut · doi:10.1136/gut.29.12.1666 · Cited by 56 · see citing works
Gastrointestinal effects and energy value of polydextrose in healthy nonobese men
Achour, L., Flourié, B., Briet, F., Pellier, P., Marteau, P., & Rambaud, J. (1994) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/59.6.1362 · Cited by 52 · see citing works
Caloric utilization and disposition of [14C]polydextrose in the rat
Figdor, S. K., & Rennhard, H. H. (1981) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00108a022 · Cited by 51 · see citing works
Short-chain fatty acids induce cell cycle inhibitors in colonocytes☆
Wang, J., & Friedman, E. A. (1998) · Gastroenterology · doi:10.1016/s0016-5085(98)70313-0 · Cited by 43 · see citing works
Koutsou, G. A., Storey, D. M., Lee, A., Zumbé, A., Flourié, B., Bot, Y. L., & Olivier, P. H. (1996) · PubMed · Cited by 42 · see citing works
Butyric acid: a role in cancer prevention
German, J. B. (1999) · Nutrition Bulletin · doi:10.1111/j.1467-3010.1999.tb00910.x · Cited by 42 · see citing works
Livesey, G., Johnson, I. T., Gee, J. M. W., Smith, T., Lee, W. E., Hillan, K. A., Meyer, J., & Turner, S. (1993) · PubMed · Cited by 29 · see citing works
Deoxyribonucleic acid base composition of certain species of the genus Bacteroides
Reddy, C. A., & Bryant, M. P. (1977) · Canadian Journal of Microbiology · doi:10.1139/m77-187 · Cited by 29 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [16]Hilton, E., P. Kolakowski, C. Singer, and M. Smith. 1997. Efficacy of Lactobacillus GG as a diarrheal preventive in travelers. J. Travel Med. 4:41-43. [DOI] [PubMed] [Google Scholar]
Cited by
Sorted by citations
Nondigestible carbohydrates, butyrate, and butyrate-producing bacteria
Fu, X., Liu, Z., Zhu, C., Mou, H., & Kong, Q. (2019) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2018.1542587 · Cited by 497 · see citing works
Lindsay, J. O. (2006) · Gut · doi:10.1136/gut.2005.074971 · Cited by 471 · see citing works
Benjamin, J. L., Hedin, C. R. H., Koutsoumpas, A., Ng, S. C., McCarthy, N. E., Hart, A. L., Kamm, M. A., Sanderson, J. D., Knight, S. C., Forbes, A., et al. (2011) · Gut · doi:10.1136/gut.2010.232025 · Cited by 361 · see citing works
Prebiotics and other microbial substrates for gut functionality
Ouwehand, A. C., Derrien, M., de Vos, W., Tiihonen, K., & Rautonen, N. (2005) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2005.01.007 · Cited by 192 · see citing works
Self-citationStenman, L. K., Lehtinen, M. J., Meland, N., Christensen, J. E., Yeung, N., Saarinen, M. T., Courtney, M., Burcelin, R., Lähdeaho, M.-L., Linros, J., et al. (2016) · EBioMedicine · doi:10.1016/j.ebiom.2016.10.036 · Cited by 177 · see citing works
In Vitro Prebiotic Evaluation of Exopolysaccharides Produced by Marine Isolated Lactic Acid Bacteria
Hongpattarakere, T., Cherntong, N., Wichienchot, S., Kolida, S., & Rastall, R. A. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.08.085 · Cited by 158 · see citing works
Magro, D. O., de Oliveira, L. M. R., Bernasconi, I., de Souza Ruela, M., Credidio, L., Barcelos, I. K., Leal, R. F., Ayrizono, M. D. L. S., Fagundes, J. J., Teixeira, L. D. B., et al. (2014) · Nutrition Journal · doi:10.1186/1475-2891-13-75 · Cited by 105 · see citing works
Effects of polydextrose supplementation on different faecal parameters in healthy volunteers
Hengst, C., Ptok, S., Roessler, A., Fechner, A., & Jahreis, G. (2009) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637480802526760 · Cited by 63 · see citing works
- Authors
- Probert, H. M., Apajalahti, J. H. A., Rautonen, N., Stowell, J., & Gibson, G. R.
- Affiliations
- University of Reading (GB)
- Danisco Innovation, FIN-02460 Kantvik, Finland
- Danisco Sweeteners, Redhill, United Kingdom
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-08
- Volume
- 70
- Issue
- 8
- Pages
- 4505-4511
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC492322
- OpenAlex
- W2167949743
- Cited by
- 147
- References
- 39
- Citations by year
- 2024: 1 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 6 · 2017: 8 · 2016: 6 · 2015: 9 · 2014: 9 · 2013: 3 · 2012: 5
- FWCI
- 8.70
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- polydextrose, lactitol, fructooligosaccharides, colonic fermentation, Bifidobacterium, Bifidobacterium infantis, short-chain fatty acids, butyrate, lactobacilli, Bacteroides, bifidogenic effect, prebiotics, bacterial community composition
- MeSH terms
- Bacteria, Bifidobacterium, Colon, Culture Media, Fatty Acids, Volatile, Fermentation, Glucans, Humans, Oligosaccharides, Species Specificity, Sugar Alcohols, Polymerase Chain Reaction, In Situ Hybridization, Fluorescence, Ecosystem
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