Cited by 799Escherichia coli research studiesEnterobacteriaceae and Cronobacter ResearchBacterial Genetics and Biotechnology

The Link between Phylogeny and Virulence in Escherichia coli Extraintestinal Infection

Open access
Picard, Bertrand, Garcia, José Sevali, Gouriou, Stéphanie, Duriez, Patrick, Brahimi, Naïma, Bingen, Edouard, Elion, Jacques, Denamur, Erick·1999·Infection and Immunity

Abstract

Previous studies suggesting a link between Escherichia coli phylogenetic groups and extraintestinal virulence have been hampered by the difficulty in establishing the intrinsic virulence of a bacterial strain. Indeed, unidentified virulence factors do exist, and the susceptibility of the host to infection is highly variable. To overcome these difficulties, we have developed a mouse model of extraintestinal virulence to test the virulence of the strains under normalized conditions. We then assessed the phylogenetic relationships compared to the E. coli reference (ECOR) collection, the presence of several known virulence determinants, and the lethality to mice of 82 human adult E. coli strains isolated from normal feces and during the course of extraintestinal infections. Commensal strains belong mainly to phylogenetic groups A and B1, are devoid of virulence determinants, and do not kill the mice. Strains exhibiting the same characteristics as the commensal strains can be isolated under pathogenic conditions, thus indicating the role of host-dependent factors, such as susceptibility linked to underlying disease, in the development of infection. Some strains of phylogenetic groups A, B1, and D are able to kill the mice, their virulence being most often correlated with the presence of virulence determinants. Lastly, strains of the B2 phylogenetic group represent a divergent lineage of highly virulent strains which kill the mice at high frequency and possess the highest level of virulence determinants. The observed link between virulence and phylogeny could correspond to the necessity of virulence determinants in a genetic background that is adequate for the emergence of a virulent clone, an expression of the interdependency of pathogenicity and metabolic activities in pathogenic bacteria.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/iai.67.2.546-553.1999

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. [21]Lebart L, Morineau A, Warwick K M. Multivariate descriptive analysis: correspondence analysis and related technique for large matrices. New York, N.Y: Wiley-Interscience; 1984. [Google Scholar]

Cited by

Sorted by citations

Authors
Picard, B., Garcia, J. S., Gouriou, S., Duriez, P., Brahimi, N., Bingen, E., Elion, J., & Denamur, E.
Affiliations
  • Centre Hospitalier Régional Universitaire de Brest (FR)
  • <!--label omitted: 2-->Laboratoire de Microbiologie, Hôpital Cochin, Assistance Publique—Hôpitaux de Paris,2
  • Inserm (FR)
  • <!--label omitted: 4-->Laboratoire de Recherche en Bactériologie Pédiatrique (JE 2255), Université Denis Diderot-Paris 7,4 Hôpital Robert Debré, Paris, France
  • <!--label omitted: 3-->INSERM U 4583 and
Venue
Infection and Immunity
Published
1999-02
Volume
67
Issue
2
Pages
546-553
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W2125201618
Cited by
794
References
42
Citations by year
2026: 13 · 2025: 17 · 2024: 32 · 2023: 27 · 2022: 43 · 2021: 39 · 2020: 46 · 2019: 33 · 2018: 29 · 2017: 34 · 2016: 39 · 2015: 46 · 2014: 52 · 2013: 38 · 2012: 36
FWCI
8.54
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Escherichia coli, phylogenetic groups, extraintestinal infections, bacterial virulence, virulence factors, commensal strains, host susceptibility, pathogenicity, virulent clones
MeSH terms
Adult, Animals, Disease Models, Animal, Escherichia coli, Escherichia coli Infections, Female, Humans, Phylogeny, Virulence, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review