The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Functional Heterogeneity and High Frequencies of Cytomegalovirus-Specific CD8+T Lymphocytes in Healthy Seropositive Donors
Open accessAbstract
Human cytomegalovirus (HCMV) infection is largely asymptomatic in the immunocompetent host, but remains a major cause of morbidity in immunosuppressed individuals. Using the recently described technique of staining antigen-specific CD8+T cells with peptide-HLA tetrameric complexes, we have demonstrated high levels of antigen-specific cells specific for HCMV peptides and show that this may exceed 4% of CD8+T cells in immunocompetent donors. Moreover, by staining with tetramers in combination with antibodies to cell surface markers and intracellular cytokines, we demonstrate functional heterogeneity of HCMV-specific populations. A substantial proportion of these are effector cytotoxic T lymphocytes, as demonstrated by their ability to lyse peptide-pulsed targets in “fresh” killing assays. These data suggest that the immune response to HCMV is periodically boosted by a low level of HCMV replication and that sustained immunological surveillance contributes to the maintenance of host-pathogen homeostasis. These observations should improve our understanding of the immunobiology of persistent viral infection.
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Cites
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“Our results, together with those obtained with intracellular cytokine assays (18) and tetramer staining of CMV-specific CTLs in patients with HIV infection (17), suggest that large populations of HCMV-specific CD81 T lymphocytes explain this increment in the lymphocyte count.”
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Cited by
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HIV-specific CD8(+) T cells produce antiviral cytokines but are impaired in cytolytic function
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Self-citationFour functionally distinct populations of human effector-memory CD8+ T lymphocytes
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Self-citationMemory T cell homeostasis and senescence during aging
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New tools for classification and monitoring of autoimmune diseases
Maecker, H. T., Lindstrom, T. M., Robinson, W. H., Utz, P. J., Hale, M., Boyd, S. D., Shen-Orr, S. S., & Fathman, C. G. (2012) · Nature Reviews Rheumatology · doi:10.1038/nrrheum.2012.66 · Cited by 95
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Self-citationImpact of CMV upon immune aging: facts and fiction
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Metadata
- Authors
- Gillespie, G. M. A., Wills, M. R., Appay, V., O'Callaghan, C., Murphy, M., Smith, N., Sissons, P., Rowland-Jones, S., Bell, J. I., & Moss, P. A. H.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2000-09
- Volume
- 74
- Issue
- 17
- Pages
- 8140-8150
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2161911625
- Cited by
- 420
- References
- 2
- FWCI
- 25.24
- Type
- journal article
- Language
- en
- Open access
- green
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