Cited by 100DNA Repair MechanismsDNA and Nucleic Acid ChemistryGenetic factors in colorectal cancer

Separate domains of Rev1 mediate two modes of DNA damage bypass in mammalian cells

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Jansen, Jacob G., Tsaalbi-Shtylik, Anastasia, Hendriks, Giel, Gali, Himabindu, Hendel, Ayal, Johansson, Fredrik, Erixon, Klaus, Livneh, Zvi +3 more·2009·Molecular and Cellular Biology

Abstract

The Y family DNA polymerase Rev1 has been proposed to play a regulatory role in the replication of damaged templates. To elucidate the mechanism by which Rev1 promotes DNA damage bypass, we have analyzed the progression of replication on UV light-damaged DNA in mouse embryonic fibroblasts that contain a defined deletion in the N-terminal BRCT domain of Rev1 or that are deficient for Rev1. We provide evidence that Rev1 plays a coordinating role in two modes of DNA damage bypass, i.e., an early and a late pathway. The cells carrying the deletion in the BRCT domain are deficient for the early pathway, reflecting a role of the BRCT domain of Rev1 in mutagenic translesion synthesis. Rev1-deficient cells display a defect in both modes of DNA damage bypass. Despite the persistent defect in the late replicational bypass of fork-blocking (6-4)pyrimidine-pyrimidone photoproducts, overall replication is not strongly affected by Rev1 deficiency. This results in almost completely replicated templates that contain gaps encompassing the photoproducts. These gaps are inducers of DNA damage signaling leading to an irreversible G(2) arrest. Our results corroborate a model in which Rev1-mediated DNA damage bypass at postreplicative gaps quenches irreversible DNA damage responses.

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  1. [42]Nelson, J. R., P. E. M. Gibbs, A. M. Nowicka, D. C. Hinkle, and C. W. Lawrence. 2000. Evidence for a second function for Saccharomyces cerevisiae Rev1p. Mol. Microbiol. 37549-554. [DOI] [PubMed] [Google Scholar]
  2. [60]Van Zeeland, A. A., and A. R. Filon. 1982. Post-replication repair: elongation of daughter strand DNA in UV-irradiated mammalian cells in culture. Prog. Mutat. Res. 4375-384. [Google Scholar]

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Authors
Jansen, J. G., Tsaalbi-Shtylik, A., Hendriks, G., Gali, H., Hendel, A., Johansson, F., Erixon, K., Livneh, Z., Mullenders, L. H. F., Haracska, L., & de Wind, N.
Affiliations
  • Leiden University Medical Center (NL)
  • Department of Toxicogenetics, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands
  • HUN-REN Szegedi Biológiai Kutatóközpont (HU)
  • Weizmann Institute of Science (IL)
  • Stockholm University (SE)
Venue
Molecular and Cellular Biology
Published
2009-06-01
Volume
29
Issue
11
Pages
3113-3123
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2110668790
Cited by
99
References
67
Citations by year
2026: 1 · 2025: 2 · 2024: 5 · 2023: 3 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 11 · 2016: 7 · 2015: 10 · 2014: 6 · 2013: 8 · 2012: 12
FWCI
4.57
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute (55005612)
  • Flight Attendant Medical Research Institute
  • European Commission (512113)
  • Nemzeti Kutatási és Technológiai Hivatal
  • Israel Science Foundation
  • KWF Kankerbestrijding (512113)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
Keywords
Rev1, Y-family DNA polymerases, DNA damage bypass, BRCT domain, UV-induced DNA damage, translesion synthesis, G2 cell cycle arrest, DNA damage signaling, mouse embryonic fibroblasts
MeSH terms
Y-Family DNA Polymerases, Animals, DNA, DNA Damage, DNA-Directed DNA Polymerase, Embryo, Mammalian, Fibroblasts, Mutation, Nucleotidyltransferases, Pyrimidine Dimers, Ultraviolet Rays, Signal Transduction, G2 Phase, S Phase, Protein Structure, Tertiary, Mice

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