Separate domains of Rev1 mediate two modes of DNA damage bypass in mammalian cells
Open accessAbstract
The Y family DNA polymerase Rev1 has been proposed to play a regulatory role in the replication of damaged templates. To elucidate the mechanism by which Rev1 promotes DNA damage bypass, we have analyzed the progression of replication on UV light-damaged DNA in mouse embryonic fibroblasts that contain a defined deletion in the N-terminal BRCT domain of Rev1 or that are deficient for Rev1. We provide evidence that Rev1 plays a coordinating role in two modes of DNA damage bypass, i.e., an early and a late pathway. The cells carrying the deletion in the BRCT domain are deficient for the early pathway, reflecting a role of the BRCT domain of Rev1 in mutagenic translesion synthesis. Rev1-deficient cells display a defect in both modes of DNA damage bypass. Despite the persistent defect in the late replicational bypass of fork-blocking (6-4)pyrimidine-pyrimidone photoproducts, overall replication is not strongly affected by Rev1 deficiency. This results in almost completely replicated templates that contain gaps encompassing the photoproducts. These gaps are inducers of DNA damage signaling leading to an irreversible G(2) arrest. Our results corroborate a model in which Rev1-mediated DNA damage bypass at postreplicative gaps quenches irreversible DNA damage responses.
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If ssDNA gaps are left unfilled, checkpoint signals are activated and lead to G2-phase cell cycle arrest.
“If the ssDNA gaps are left unfilled, checkpoint signals are activated leading to a G2-phase cell cycle arrest [17][18][19].”
From Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome (2014) · cites this paper as [18]
In UVC-irradiated XP-C cells, ATR activation might be triggered by accumulation of ssDNA gaps during replication, leading to mild and temporary G2-phase arrest.
“In UVC-irradiated XP-C cells, ATR activation might be triggered by the accumulation of ssDNA gaps during DNA replication, leading to a mild and temporary G2-phase arrest, in agreement with previous work [18,19,24,50]”
From Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome (2014) · cites this paper as [18]
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- Authors
- Jansen, J. G., Tsaalbi-Shtylik, A., Hendriks, G., Gali, H., Hendel, A., Johansson, F., Erixon, K., Livneh, Z., Mullenders, L. H. F., Haracska, L., & de Wind, N.
- Affiliations
- Leiden University Medical Center (NL)
- Department of Toxicogenetics, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands
- HUN-REN Szegedi Biológiai Kutatóközpont (HU)
- Weizmann Institute of Science (IL)
- Stockholm University (SE)
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2009-06-01
- Volume
- 29
- Issue
- 11
- Pages
- 3113-3123
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC2682010
- OpenAlex
- W2110668790
- Cited by
- 99
- References
- 67
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 5 · 2023: 3 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 11 · 2016: 7 · 2015: 10 · 2014: 6 · 2013: 8 · 2012: 12
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute (55005612)
- Flight Attendant Medical Research Institute
- European Commission (512113)
- Nemzeti Kutatási és Technológiai Hivatal
- Israel Science Foundation
- KWF Kankerbestrijding (512113)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Keywords
- Rev1, Y-family DNA polymerases, DNA damage bypass, BRCT domain, UV-induced DNA damage, translesion synthesis, G2 cell cycle arrest, DNA damage signaling, mouse embryonic fibroblasts
- MeSH terms
- Y-Family DNA Polymerases, Animals, DNA, DNA Damage, DNA-Directed DNA Polymerase, Embryo, Mammalian, Fibroblasts, Mutation, Nucleotidyltransferases, Pyrimidine Dimers, Ultraviolet Rays, Signal Transduction, G2 Phase, S Phase, Protein Structure, Tertiary, Mice
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