Ectopic Expression of Hel-N1, an RNA-Binding Protein, Increases Glucose Transporter (GLUT1) Expression in 3T3-L1 Adipocytes
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3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member of the ELAV-like family of proteins associated with the induction and maintenance of differentiation in various species. ELAV proteins are known to bind in vitro to short stretches of uridylates in the 3' untranslated regions (3'UTRs) of unstable mRNAs encoding growth-regulatory proteins involved in transcription and signal transduction. GLUT1 mRNA also contains a large 3'UTR with a U-rich region that binds specifically to Hel-N1 in vitro. Analysis of the altered GLUT1 expression at the translational and posttranscriptional levels suggested a mechanism involving both mRNA stabilization and accelerated formation of translation initiation complexes. These findings are consistent with the hypothesis that the Hel-N1 family of proteins modulate gene expression at the level of mRNA in the cytoplasm.
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HuB enhances GLUT1 mRNA half-life and translation.
“The initial demonstration that Hu proteins regulate mRNA stability was based on the observation that HuB enhances GLUT1 mRNA half-life and, incidentally, translation (Jain et al. 1997).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Jain et al. 1997
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- Authors
- Jain, R. G., Andrews, L. G., McGowan, K. M., Pekala, P. H., & Keene, J. D.
- Affiliations
- East Carolina University (US)
- Duke Medical Center (US)
- Corresponding author
- Pekala, Phillip H.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1997-02
- Volume
- 17
- Issue
- 2
- Pages
- 954-962
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC231821
- OpenAlex
- W2122744980
- Cited by
- 199
- References
- 1
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 7 · 2013: 6 · 2012: 1
- FWCI
- 7.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The Scholarship East Carolina University's Institutional Repository (East Carolina University) (submittedVersion)
- Funders
- North Carolina Biotechnology Center
- National Institutes of Health (5r01ca060083-04, 5t32mh015177-19, 5r01gm032892-10)
- National Institute of Mental Health (T32MH15177)
- National Cancer Institute (CA60083)
- National Institute of General Medical Sciences (GM32892)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- RNA-binding proteins, ELAV-like proteins, GLUT1, glucose transporters, 3T3-L1 preadipocytes, adipocytes, glucose uptake, mRNA stabilization, 3' untranslated region, translational regulation, post-transcriptional regulation, adipogenesis
- MeSH terms
- ELAV-Like Protein 2, Animals, Base Sequence, Cell Differentiation, Cell Membrane, DNA, Gene Expression Regulation, Molecular Sequence Data, Monosaccharide Transport Proteins, Muscle Proteins, Nerve Tissue Proteins, Polyribosomes, RNA, Messenger, Transfection, 3T3 Cells, RNA-Binding Proteins, Adipocytes, Glucose Transporter Type 1, Glucose Transporter Type 4, Mice, ELAV Proteins
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