Diverse molecular functions of Hu proteins

Hinman, M. N.; Lou, H.

doi:10.1007/s00018-008-8252-6

Cited by 587RNA Research and SplicingRNA regulation and diseaseRNA and protein synthesis mechanisms

Diverse molecular functions of Hu proteins

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Hinman, M. N., Lou, H.·2008·Cellular and Molecular Life Sciences

DOI 10.1007/s00018-008-8252-6PMC PMC2580827

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Abstract

Hu proteins are RNA-binding proteins involved in diverse biological processes. The neuronal members of the Hu family, HuB, HuC, and HuD play important roles in neuronal differentiation and plasticity, while the ubiquitously expressed family member, HuR, has numerous functions mostly related to cellular stress response. The pivotal roles of Hu proteins are dictated by their molecular functions affecting a large number of target genes. Hu proteins affect many post-transcriptional aspects of RNA metabolism, from splicing to translation. In this communication, we will focus on these molecular events and review our current understanding of how Hu proteins mediate them. In particular, emphasis will be put on the nuclear functions of these proteins, which were recently discovered. Three examples including calcitonin/calcitonin gene-related peptide, neurofibromatosis type 1, and Ikaros will be discussed in detail. In addition, an intriguing theme of antagonism between Hu proteins and other AU-rich sequence binding proteins will be discussed.

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There are a few known AUBPs that enhance mRNA stability, with Hu proteins being the most notable.
“Nevertheless, there are a few known AUBPs that enhance mRNA stability, with Hu proteins being the most notable (Brennan and Steitz 2001; Hinman and Lou 2008; Pascale et al. 2008).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Hinman and Lou 2008
HuD mediates alternative splicing of the Ikaros gene, possibly by suppressing exon 4 inclusion.
“Furthermore, overexpression and down-regulation of HuD in T-cell lines demonstrated that it mediates alternative splicing of the Ikaros gene, possibly by suppressing exon 4 inclusion (Bellavia et al. 2007; Hinman and Lou 2008).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Hinman and Lou 2008
The ELAVL family consists of four highly homologous members.
“The ELAVL (also known as Hu) family of proteins consists of four highly homologous members109–111”
From The neurogenetics of alternative splicing (2016) · cites this paper as [111]
ELAVLs regulate mRNA stability and translation efficiency through binding to 3′ UTRs.
“The ELAVLs have primarily been studied as regulators of mRNA stability and translation efficiency through their binding to 3′ UTRs110,111,123”
From The neurogenetics of alternative splicing (2016) · cites this paper as [111]

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In vivo post‐transcriptional regulation of GAP‐43 mRNA by overexpression of the RNA‐binding protein HuD
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ELAV
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Self-citation
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Regulation of mRNA Translation and Stability by microRNAs
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Authors: Hinman, M. N., & Lou, H.
Affiliations: Case Western Reserve University (US)
Case Comprehensive Cancer Center (US)
Corresponding author: Lou, H.
ORCID: Hinman, M. N.
Venue: Cellular and Molecular Life Sciences
Publisher: Springer Science and Business Media LLC
Published: 2008-06-26
Volume: 65
Issue: 20
Pages: 3168-3181
ISSN: 1420-682X (ISSN-L)
1420-9071
DOI: 10.1007/s00018-008-8252-6
PMC: PMC2580827
OpenAlex: W2064492560
Cited by: 587
References: 116
Citations by year: 2026: 12 · 2025: 23 · 2024: 30 · 2023: 36 · 2022: 26 · 2021: 26 · 2020: 35 · 2019: 30 · 2018: 35 · 2017: 29 · 2016: 34 · 2015: 26 · 2014: 37 · 2013: 45 · 2012: 44
FWCI: 16.16
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Cellular and Molecular Life Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute of General Medical Sciences (T32GM008613)
National Institute of Neurological Disorders and Stroke (R01NS049103, NS-049103)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords: HU protein, RNA-binding proteins, HuR, hubs, HuC, HUD, post-transcriptional regulation, RNA splicing, translation (biology), neuronal differentiation, neuroplasticity, cellular stress response, calcitonin, calcitonin gene-related peptide, neurofibromatosis type 1, Ikaros, AU-rich elements
MeSH terms: Animals, Brain, Cell Nucleus, Humans, Protein Biosynthesis, Calcitonin Gene-Related Peptide, RNA Stability, ELAV Proteins

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