The DOI matches its Crossref record; 46 of the 47 references it cites were found in a registry or an index.
Dose-dependent responses of myofibrillar protein synthesis with beef ingestion are enhanced with resistance exercise in middle-aged men
Open accessAbstract
Aging impairs the sensitivity of skeletal muscle to anabolic stimuli, such as amino acids and resistance exercise. Beef is a nutrient-rich source of dietary protein capable of stimulating muscle protein synthesis (MPS) rates in older men at rest. To date, the dose–response of myofibrillar protein synthesis to graded ingestion of protein-rich foods, such as beef, has not been determined. We aimed to determine the dose–response of MPS with and without resistance exercise to graded doses of beef ingestion. Thirty-five middle-aged men (59 ± 2 years) ingested 0 g, 57 g (2 oz; 12 g protein), 113 g (4 oz; 24 g protein), or 170 g (6 oz; 36 g protein) of (15% fat) ground beef (n = 7 per group). Subjects performed a bout of unilateral resistance exercise to allow measurement of the fed state and the fed plus resistance exercise state within each dose. A primed constant infusion of l-[1-13 C]leucine was initiated to measure leucine oxidation and of l-[ring-13 C 6]phenylalanine was initiated to measure myofibrillar MPS. Myofibrillar MPS was increased with ingestion of 170 g of beef to a greater extent than all other doses at rest and after resistance exercise. There was more leucine oxidation with ingestion of 113 g of beef than with 0 g and 57 g, and it increased further after ingestion of 170 g of beef (all p < 0.05). Ingestion of 170 g of beef protein is required to stimulate a rise in myofibrillar MPS over and above that seen with lower doses. An isolated bout of resistance exercise was potent in stimulating myofibrillar MPS, and acted additively with feeding.
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“the observation of a dose-responsive relationship between protein intake and MPS82–85”
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“In contrast to the findings of Cuthbertson et al. (2005), our data suggest that older men are less sensitive to lower doses (<10 g) of protein than the young — a finding that is in line with recent findings using whey protein (Yang et al. 2012).”
InfluentialMoore, D. R., Robinson, M. J., Fry, J. L., Tang, J. E., Glover, E. I., Wilkinson, S. B., Prior, T., Tarnopolsky, M. A., & Phillips, S. M. (2009) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.2008.26401 · Cited by 973
Self-citationKatsanos, C. S., Kobayashi, H., Sheffield-Moore, M., Aarsland, A., & Wolfe, R. R. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00488.2005 · Cited by 923
An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein
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“Studies have consistently demonstrated that resistance exercise increases MPS in older adults (Yarasheski et al. 1993; Cuthbertson et al. 2005; Drummond et al. 2008), although the response is blunted, compared with that in younger adults (Kumar et al. 2009).”
Wilkinson, S. B., Tarnopolsky, M. A., MacDonald, M. J., MacDonald, J. R., Armstrong, D., & Phillips, S. M. (2007) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/85.4.1031 · Cited by 530
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Protein content and amino acid composition of commercially available plant-based protein isolates
Gorissen, S. H. M., Crombag, J. J. R., Senden, J. M. G., Waterval, W. A. H., Bierau, J., Verdijk, L. B., & van Loon, L. J. C. (2018) · Amino Acids · doi:10.1007/s00726-018-2640-5 · Cited by 1,169
Morton, R. W., Murphy, K. T., McKellar, S. R., Schoenfeld, B. J., Henselmans, M., Helms, E., Aragon, A. A., Devries, M. C., Banfield, L., Krieger, J. W., et al. (2018) · British Journal of Sports Medicine · doi:10.1136/bjsports-2017-097608 · Cited by 1,269
Self-citationThe Skeletal Muscle Anabolic Response to Plant- versus Animal-Based Protein Consumption
van Vliet, S., Burd, N. A., & van Loon, L. J. (2015) · The Journal of Nutrition · doi:10.3945/jn.114.204305 · Cited by 651
Self-citationWitard, O. C., Jackman, S. R., Breen, L., Smith, K., Selby, A., & Tipton, K. D. (2014) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.112.055517 · Cited by 517
Self-citationPaddon‐Jones, D., Campbell, W. W., Jacques, P. F., Kritchevsky, S. B., Moore, L. L., Rodriguez, N. R., & van Loon, L. J. C. (2015) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.114.084061 · Cited by 284
Metadata
- Authors
- Robinson, M. J., Burd, N. A., Breen, L., Rerecich, T., Yang, Y., Hector, A. J., Baker, S. K., & Phillips, S. M.
- Affiliations
- McMaster University (CA)
- Venue
- Applied Physiology, Nutrition, and Metabolism
- Publisher
- Canadian Science Publishing
- Published
- 2013-02
- Volume
- 38
- Issue
- 2
- Pages
- 120-125
- ISSN
- 1715-5312 (ISSN-L)
- 1715-5320
- OpenAlex
- W2071076747
- Cited by
- 109
- References
- 47
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 8 · 2021: 7 · 2020: 12 · 2019: 9 · 2018: 9 · 2017: 9 · 2016: 17 · 2015: 10 · 2014: 8 · 2013: 7
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied Physiology Nutrition and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Funders
- National Cattlemen's Beef Association
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Nutrition and Health in Aging
- Keywords
- myofibrillar protein synthesis, resistance training, dose-response relationship, dietary protein, leucine oxidation, skeletal muscle anabolism, middle-aged men, aging, fed state
- MeSH terms
- Amino Acids, Animals, Cattle, Gene Expression Regulation, Humans, Male, Meat, Middle Aged, Muscle Proteins, Exercise
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