Antioxidative and free radical scavenging effects of ecdysteroids from Serratula strangulata
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The antioxidative and free radical scavenging effects of four ecdysteroids, 20-hydroxyecdysone (E1), 25-deoxy-11,20-dihydroxyecdysone (E2), 24-(2-hydroxyethyl)-20-hydroxyecdysone (E3), and 20-hydroxyecdysone-20,22-monoacetonide (E4), isolated from the Chinese herb Serratula strangulata have been investigated in vitro. These ecdysteroids could protect human erythrocytes against oxidative hemolysis induced by a water-soluble azo initiator 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH). They could also inhibit the peroxidation of rat liver microsomes induced by hydroxyl radicals, as monitored by the formation of thiobarbituric acid reactive substances (TBARS), and prevent radical-induced decrease of membrane fluidity as determined by fluorescence polarization. They reacted with galvinoxyl radicals in homogeneous solution, and the pseudo-first-order rate constants were determined spectrophotometrically by following the disappearance of galvinoxyl radicals. Compounds E1 and (or) E3 were the most active in both antioxidative and radical-scavenging reactions.
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Several published reports have written about the ROS-scavenger and antioxidant properties of 20E in several in vitro and in vivo systems.
“Several published reports have written about the ROS-scavenger and antioxidant properties of 20E in several in vitro [30,31] and in vivo systems [32,33].”
From 20-Hydroxyecdysone Confers Antioxidant and Antineoplastic Properties in Human Non-Small Cell Lung Cancer Cells (2023) · cites this paper as [31]
20E has previously been shown to possess antioxidant capacities in both in vitro systems and non-cancer cells.
“As 20E has previously been shown to possess antioxidant capacities in both in vitro systems and non-cancer cells [30,31,32]”
From 20-Hydroxyecdysone Confers Antioxidant and Antineoplastic Properties in Human Non-Small Cell Lung Cancer Cells (2023) · cites this paper as [31]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Free radicals in biology and medicine
Gutteridge, J. M. C. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90478-o · Cited by 17,178 · see citing works
[30] Microsomal lipid peroxidation
Buege, J. A., & Aust, S. D. (1978) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(78)52032-6 · Cited by 13,437 · see citing works
Cancer chemopreventive activity of resveratrol, a natural product derived from grapes
Jang, M., Cai, L., Udeani, G. O., Slowing, K. V., Thomas, C. F., Beecher, C. W. W., Fong, H. H. S., Farnsworth, N. R., Kinghorn, A. D., Mehta, R. G., et al. (1997) · Science · doi:10.1126/science.275.5297.218 · Cited by 4,935 · cited 1× · see citing works
“Plant- and food-derived antioxidants, such as green-tea polyphenols and resveratrol, have been reported to be beneficial in protecting against these diseases (Ahmad and Mukhtar 1999; Jankun et al. 1997; Jang et al. 1997); hence, antioxidant therapy has become an attractive therapeutic strategy (Rice-Evans et al.”
BackgroundHandbook of methods for oxygen radical research
Greenwald, R. A. (1987) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(87)80012-6 · Cited by 2,384 · see citing works
Oxidative damage and mitochondrial decay in aging.
Shigenaga, M. K., Hagen, T. M., & Ames, B. N. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.23.10771 · Cited by 2,055 · see citing works
Antioxidant and redox regulation of gene transcription
Sen, C. K., & Packer, L. (1996) · The FASEB Journal · doi:10.1096/fasebj.10.7.8635688 · Cited by 1,996 · see citing works
Marnett, L. J. (2000) · Carcinogenesis · doi:10.1093/carcin/21.3.361 · Cited by 1,887 · see citing works
The biochemistry of programmed cell death
Kroemer, G., PETIT, P. X., Zamzami, N., Vayssière, J., & Mignotte, B. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.13.7557017 · Cited by 992 · see citing works
Shinitzky, M., & Barenholz, Y. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42780-4 · Cited by 865 · see citing works
Why drinking green tea could prevent cancer
Jankun, J., Selman, S. H., Swiercz, R., & Skrzypczak‐Jankun, E. (1997) · Nature · doi:10.1038/42381 · Cited by 720 · see citing works
Antioxidative and free radical scavenging activities of selected medicinal herbs
Liu, F., & Ng, T. B. (2000) · Life Sciences · doi:10.1016/s0024-3205(99)00643-8 · Cited by 504 · see citing works
Jenkins, R. R. (1988) · Sports Medicine · doi:10.2165/00007256-198805030-00003 · Cited by 470 · see citing works
Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins
Abuja, P. M., & Albertini, R. (2001) · Clinica Chimica Acta · doi:10.1016/s0009-8981(01)00393-x · Cited by 454 · see citing works
Current status of antioxidant therapy
Rice-Evans, C. A., & Diplock, A. T. (1993) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(93)90127-g · Cited by 428 · see citing works
Phytoecdysteroids: Biological aspects
Dinan, L. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00078-4 · Cited by 392 · cited 1× · see citing works
“Ecdysteroids were initially recognized as steroidal hormones controlling moulting and metamorphosis in insects, and it was realized recently that these steroids are present in all stages of insect development, regulating many biochemical and physiological processes (Dinan 2001).”
BackgroundGreen Tea Polyphenols and Cancer: Biologic Mechanisms and Practical Implications
Ahmad, N., & Mukhtar, H. (1999) · Nutrition Reviews · doi:10.1111/j.1753-4887.1999.tb06927.x · Cited by 373 · see citing works
Miki, M., Tamai, H., Mino, M., Yamamoto, Y., & Niki, E. (1987) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(87)90358-4 · Cited by 362 · see citing works
Nonaka, G., KAWAHARA, O., & Nishioka, I. (1983) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.31.3906 · Cited by 272 · see citing works
Effect of phytyl side chain of vitamin E on its antioxidant activity.
Niki, E., Kawakami, A., Saito, M., et al. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89536-9 · Cited by 219 · see citing works
Liu, Z., Ma, L., Zhou, B., Li, Y., & Liu, Z. (2000) · Chemistry and Physics of Lipids · doi:10.1016/s0009-3084(00)00133-x · Cited by 147 · see citing works
Self-citationChattopadhyay, A., Choudhury, T. D., Bandyopadhyay, D., et al. (2000) · Biochemical Pharmacology · doi:10.1016/s0006-2952(99)00277-4 · Cited by 145 · see citing works
Nakanishi, K., Koreeda, M., Sasaki, S., Chang, M. L., & Hsu, H. Y. (1966) · Chemical Communications (London) · doi:10.1039/c19660000915 · Cited by 121 · see citing works
Effect of β-carotene and α-tocopherol on radical-initiated peroxidation of microsomes
Palozza, P., Moulla, S., & Krinsky, N. I. (1992) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(92)90074-q · Cited by 118 · see citing works
Cancer Chemopreventive Agents (Antitumor-promoters) from <i>Ajuga </i><i>d</i><i>ecumbens</i>
Takasaki, M., Tokuda, H., Nishino, H., & Konoshima, T. (1999) · Journal of Natural Products · doi:10.1021/np990033w · Cited by 83 · see citing works
Liebler, D. C., Stratton, S. P., & Kaysen, K. L. (1997) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1996.9822 · Cited by 81 · see citing works
Oxidants and Antioxidants in Clinical Medicine: Past, Present and Future Potential
Bland, J. S. (1995) · Journal of Nutritional & Environmental Medicine · doi:10.3109/13590849509000226 · Cited by 80 · see citing works
Antioxidant activities of probucol against lipid peroxidations
Goton, N., Shimizu, K., Komuro, E., et al. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90300-k · Cited by 80 · see citing works
Zhou, B., Jia, Z., Chen, Z., et al. (2000) · Journal of the Chemical Society Perkin Transactions 2 · doi:10.1039/a908084h · Cited by 66 · see citing works
Sustained mammary gland‐directed, ponasterone A‐inducible expression in transgenic mice
Albanese, C., Reutens, A., Bouzahzah, B., Fu, M., D’Amico, M., Link, T., Nicholson, R., DePinho, R., & Pestell, R. (2000) · The FASEB Journal · doi:10.1096/fasebj.14.7.877 · Cited by 59 · see citing works
Tsuchiya, J., Yamada, T., Niki, E., et al. (1985) · Bulletin of the Chemical Society of Japan · doi:10.1246/bcsj.58.326 · Cited by 55 · see citing works
Inhibition of free radical induced oxidative hemolysis of red blood cells by green tea polyphenols
Ma, L., Liu, Z., Zhou, B., Yang, L., & Liu, Z. (2000) · Chinese Science Bulletin · doi:10.1007/bf03183525 · Cited by 42 · see citing works
Self-citationKuang, Z.-H., Wang, P., Zheng, R., et al. (1994) · Chemistry and Physics of Lipids · doi:10.1016/0009-3084(94)02307-7 · Cited by 38 · see citing works
20-hydroxyecdysone as a human lymphocyte and neutrophil modulator: In vitro evaluation
Trenin, D. S., & Володин, В. В. (1999) · Archives of Insect Biochemistry and Physiology · doi:10.1002/(sici)1520-6327(1999)41:3 · Cited by 33 · see citing works
Glyceroglycolipids from Serratula strangulata
Dai, J. Q., Zhu, Q. X., Zhao, C. Y., et al. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00308-9 · Cited by 27 · see citing works
Self-citationChen, Z., Zhou, B., Li, Y., et al. (2001) · Journal of the Chemical Society Perkin Transactions 2 · doi:10.1039/b101713f · Cited by 24 · see citing works
Prolactin modifies the fluidity of rat liver membranes
Dave, J. R., Knazek, R. A., & Liu, S. C. (1981) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(81)80060-5 · Cited by 23 · see citing works
Kinetic EPR studies on bio-antioxidants
Zhou, B., Chen, Z., Chen, Y., et al. (2000) · Applied Magnetic Resonance · doi:10.1007/bf03162153 · Cited by 22 · see citing works
Antioxidant activity of vitamin E and vitamin C derivatives in membrane mimetic systems
Liu, Z.-L. (1995) · Birkhäuser Basel eBooks · doi:10.1007/978-3-0348-9059-5_18 · Cited by 20 · see citing works
Self-citationESR equilibrium measurements of the OH bond energy in α‐tocopherol
Coronel, M. E. J., & Colussi, A. J. (1988) · International Journal of Chemical Kinetics · doi:10.1002/kin.550200908 · Cited by 16 · see citing works
Lüers, G. H., Jess, N., & Franz, T. (2000) · European Journal of Cell Biology · doi:10.1078/0171-9335-00086 · Cited by 15 · see citing works
2D NMR STUDY ON TEA POLYPHENOLS
Jing, D. (2002) · Bopuxue zazhi · Cited by 9 · see citing works
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Hu, J., Luo, C. X., Chu, W. H., Shan, Y. A., Qian, Z.-M., Zhu, G., Yu, Y. B., & Feng, H. (2012) · PLoS ONE · doi:10.1371/journal.pone.0050764 · Cited by 67 · see citing works
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Sun, Y., & Yasukawa, K. (2008) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2008.04.008 · Cited by 69 · see citing works
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Hu, J., Zhao, T. Z., Chu, W. H., Luo, C. X., Tang, W. H., Yi, L., & Feng, H. (2010) · Journal of Cellular Biochemistry · doi:10.1002/jcb.22877 · Cited by 26 · see citing works
Shuvalov, O., Kirdeeva, Y., Fefilova, E., Netsvetay, S., Zorin, M., Vlasova, Y., Fedorova, O., Daks, A., Parfenyev, S., & Barlev, N. (2023) · Metabolites · doi:10.3390/metabo13050656 · Cited by 21 · see citing works
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Lamhamdi, M., Lafont, R., Rharrabe, K., Sayah, F., Aarab, A., & Bakrim, A. (2016) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2015.11.006 · Cited by 18 · see citing works
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- Authors
- Cai, Y.-J., Dai, J.-Q., Fang, J.-G., Ma, L.-P., Hou, L.-F., Yang, L., & Liu, Z.-L.
- Affiliations
- Lanzhou University (CN)
- Venue
- Canadian Journal of Physiology and Pharmacology
- Publisher
- Canadian Science Publishing
- Published
- 2002-12-01
- Volume
- 80
- Issue
- 12
- Pages
- 1187-1194
- ISSN
- 0008-4212 (ISSN-L)
- 1205-7541
- OpenAlex
- W2095017505
- Cited by
- 43
- References
- 42
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 6 · 2022: 2 · 2021: 1 · 2020: 2 · 2018: 2 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- green
- 202.201.7.4:8080
- Licence
- Locations
- Canadian Journal of Physiology and Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Lanzhou University Institutional Repository (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- ecdysteroids, antioxidant activity, free radical scavenging, 20-hydroxyecdysone, AAPH, lipid peroxidation, liver microsomes, galvinoxyl radical, membrane fluidity, Chinese herbal medicine
- MeSH terms
- Animals, Antioxidants, Drugs, Chinese Herbal, Female, Humans, Microsomes, Liver, Phytosterols, Free Radical Scavengers, Rats, Wistar, Thiobarbituric Acid Reactive Substances, Asteraceae, Ecdysteroids, Rats
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