Adiponectin is expressed by skeletal muscle fibers and influences muscle phenotype and function
Open accessAbstract
Adiponectin (Ad) is linked to various disease states and mediates antidiabetic and anti-inflammatory effects. While it was originally thought that Ad expression was limited to adipocytes, we demonstrate here that Ad is expressed in mouse skeletal muscles and within differentiated L6 myotubes, as assessed by RT-PCR, Western blot, and immunohistochemical analyses. Serial muscle sections stained for fiber type, lipid content, and Ad revealed that muscle fibers with elevated intramyocellular Ad expression were consistently type IIA and IID fibers with detectably higher intramyocellular lipid (IMCL) content. To determine the effect of Ad on muscle phenotype and function, we used an Ad-null [knockout (KO)] mouse model. Body mass increased significantly in 24-wk-old KO mice [+5.5 +/- 3% relative to wild-type mice (WT)], with no change in muscle mass observed. IMCL content was significantly increased (+75.1 +/- 25%), whereas epididymal fat mass, although elevated, was not different in the KO mice compared with WT (+35.1 +/- 23%; P = 0.16). Fiber-type composition was unaltered, although type IIB fiber area was increased in KO mice (+25.5 +/- 6%). In situ muscle stimulation revealed lower peak tetanic forces in KO mice relative to WT (-47.5 +/- 6%), with no change in low-frequency fatigue rates. These data demonstrate that the absence of Ad expression causes contractile dysfunction and phenotypical changes in skeletal muscle. Furthermore, we demonstrate that Ad is expressed in skeletal muscle and that its intramyocellular localization is associated with elevated IMCL, particularly in type IIA/D fibers.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
ApN can also be a true myokine.
“Besides being an adipokine, ApN can also be a true myokine [36,37].”
From Adiponectin and Its Mimics on Skeletal Muscle: Insulin Sensitizers, Fat Burners, Exercise Mimickers, Muscling Pills … or Everything Together? (2020) · cites this paper as [37]
Muscle of ApN-KO mice showed contractile dysfunction in situ.
“Moreover, muscle of ApN-KO mice showed contractile dysfunction in situ [37].”
From Adiponectin and Its Mimics on Skeletal Muscle: Insulin Sensitizers, Fat Burners, Exercise Mimickers, Muscling Pills … or Everything Together? (2020) · cites this paper as [37]
Cites
Yamauchi, T., Kamon, J., Waki, H., Terauchi, Y., Kubota, N., Hara, K., Mori, Y., Ide, T., Murakami, K., Tsuboyama-Kasaoka, N., et al. (2001) · Nature Medicine · doi:10.1038/90984 · Cited by 4,915 · see citing works
Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in Obesity
Arita, Y., Kihara, S., Ouchi, N., Takahashi, M., Maeda, K., Miyagawa, J.-I., Hotta, K., Shimomura, I., Nakamura, T., Miyaoka, K., et al. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0255 · Cited by 4,856 · see citing works
Yamauchi, T., Kamon, J., Minokoshi, Y., Ito, Y., Waki, H., Uchida, S., Yamashita, S., Noda, M., Kita, S., Ueki, K., et al. (2002) · Nature Medicine · doi:10.1038/nm788 · Cited by 4,238 · see citing works
Cloning of adiponectin receptors that mediate antidiabetic metabolic effects
Yamauchi, T., Kamon, J., Ito, Y., Tsuchida, A., Yokomizo, T., Kita, S., Sugiyama, T., Miyagishi, M., Hara, K., Tsunoda, M., et al. (2003) · Nature · doi:10.1038/nature01705 · Cited by 3,176 · see citing works
Adiponectin and Adiponectin Receptors
Kadowaki, T., & Yamauchi, T. (2005) · Endocrine Reviews · doi:10.1210/er.2005-0005 · Cited by 2,634 · see citing works
The adipocyte-secreted protein Acrp30 enhances hepatic insulin action
Berg, A. H., Combs, T. P., Du, X., Brownlee, M., & Scherer, P. E. (2001) · Nature Medicine · doi:10.1038/90992 · Cited by 2,526 · see citing works
Diet-induced insulin resistance in mice lacking adiponectin/ACRP30
Maeda, N., Shimomura, I., Kishida, K., Nishizawa, H., Matsuda, M., Nagaretani, H., Furuyama, N., Kondo, H., Takahashi, M., Arita, Y., et al. (2002) · Nature Medicine · doi:10.1038/nm724 · Cited by 2,074 · see citing works
Ouchi, N., Kihara, S., Arita, Y., Nishida, M., Matsuyama, A., Okamoto, Y., Ishigami, M., Kuriyama, H., Kishida, K., Nishizawa, H., et al. (2001) · Circulation · doi:10.1161/01.cir.103.8.1057 · Cited by 1,275 · see citing works
Disruption of Adiponectin Causes Insulin Resistance and Neointimal Formation
Kubota, N., Terauchi, Y., Yamauchi, T., Kubota, T., Moroi, M., Matsui, J., Eto, K., Yamashita, T., Kamon, J., Satoh, H., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200251200 · Cited by 1,213 · see citing works
Pajvani, U. B., Hawkins, M., Combs, T. P., Rajala, M. W., Doebber, T., Berger, J. P., Wagner, J. A., Wu, M., Knopps, A., Xiang, A. H., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m311113200 · Cited by 1,163 · see citing works
Disruption of adiponectin causes insulin resistance and neointimal formation
Froguel, P. (2002) · HAL · Cited by 1,126 · see citing works
Yoon, M. J., Lee, G. Y., Chung, J.-J., Ahn, Y. H., Hong, S. H., & Kim, J. B. (2006) · Diabetes · doi:10.2337/db05-1322 · Cited by 546 · see citing works
Adiponectin Multimeric Complexes and the Metabolic Syndrome Trait Cluster
Lara‐Castro, C., Luo, N., Wallace, P., Klein, R. L., & Garvey, W. T. (2006) · Diabetes · doi:10.2337/diabetes.55.01.06.db05-1105 · Cited by 480 · see citing works
Xu, A., Chan, K. W., Hoo, R. L. C., Wang, Y., Tan, K. C. B., Zhang, J., Chen, B., Lam, M. C., Tse, C. A., Cooper, G. J. S., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m414231200 · Cited by 418 · see citing works
Self-citationAdiponectin is synthesized and secreted by human and murine cardiomyocytes
Piñeiro, R., Iglesias, M. J., Gallego, R., Raghay, K., Eiras, S., Rubio, J., Diéguez, C., Gualillo, O., González-Juanatey, J. R., & Lago, F. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.07.098 · Cited by 350 · see citing works
Shibata, R., Ouchi, N., Kihara, S., Sato, K., Funahashi, T., & Walsh, K. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m402558200 · Cited by 336 · see citing works
Increased β-Oxidation but No Insulin Resistance or Glucose Intolerance in Mice Lacking Adiponectin
Ma, K., Cabrero, À., Saha, P., Kojima, H., Li, L., Chang, B. H.-J., Paul, A., & Chan, L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200362200 · Cited by 284 · see citing works
Self-citationWang, Y., Lam, K. S. L., Chan, L., Chan, K. W., Lam, J. B. B., Lam, M. C., Hoo, R. L. C., Mak, W. W. N., Cooper, G. J. S., & Xu, A. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m513907200 · Cited by 254 · see citing works
Self-citationDelaigle, A. M., Jonas, J.-C., Bauche, I. B., Cornu, O., & Brichard, S. M. (2004) · Endocrinology · doi:10.1210/en.2004-0503 · Cited by 241 · see citing works
Fat content in individual muscle fibers of lean and obese subjects.
Malenfant, P., Joanisse, D., Thériault, R., Goodpaster, B., Kelley, D., & Simoneau, J.-A. (2001) · International Journal of Obesity · doi:10.1038/sj.ijo.0801733 · Cited by 227 · see citing works
Ding, G., Qin, Q., He, N., Francis, S., Hou, J., Liu, J., Ricks, E., & Yang, Q. (2007) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2007.04.014 · Cited by 150 · see citing works
Dual Roles of Adiponectin / Acrp30 In Vivo as an Anti-Diabetic and Anti- Atherogenic Adipokine
Yamauchi, T., Hara, K., Kubota, N., Terauchi, Y., Tobe, K., Froguel, P., Nagai, R., & Kadowaki, T. (2003) · Current Drug Targets - Immune Endocrine & Metabolic Disorders · doi:10.2174/1568008033340090 · Cited by 142 · see citing works
Hong, L., Rabaglia, M. E., Stoehr, J. P., Nadler, S. T., Schueler, K. L., Zou, F., Yandell, B. S., & Attie, A. (2003) · Diabetes · doi:10.2337/diabetes.52.3.688 · Cited by 141 · see citing works
Ogilvie, R. W., & Feeback, D. L. (1990) · Stain Technology · doi:10.3109/10520299009105613 · Cited by 116 · see citing works
Mechanisms regulating energy metabolism by adiponectin in obesity and diabetes
Fang, X., & Sweeney, G. (2006) · Biochemical Society Transactions · doi:10.1042/bst0340798 · Cited by 109 · see citing works
Self-citationZhou, Q., Du, J., Hu, Z., Walsh, K. X., & Wang, X. H. (2007) · Endocrinology · doi:10.1210/en.2007-0183 · Cited by 103 · see citing works
Liu, Y., Retnakaran, R. R., Hanley, A. J., et al. (2007) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2007-0890 · Cited by 86 · see citing works
Self-citationRosenblatt, J. D., Kuzon, W. M., Plyley, M. J., Pynn, B. R., & McKee, N. H. (1987) · Stain Technology · doi:10.3109/10520298709107973 · Cited by 84 · see citing works
Induction of adiponectin in skeletal muscle of type 2 diabetic mice: In vivo and in vitro studies
Delaigle, A. M., Senou, M., Guiot, Y., Many, M. C., & Brichard, S. M. (2006) · Diabetologia · doi:10.1007/s00125-006-0210-y · Cited by 79 · see citing works
Fang, X., Palanivel, R., Zhou, X., Liu, Y., Xu, A., Wang, Y., & Sweeney, G. (2005) · Journal of Molecular Endocrinology · doi:10.1677/jme.1.01877 · Cited by 76 · see citing works
Self-citationCardiac expression of adiponectin and its receptors in streptozotocin-induced diabetic rats
Guo, Z., Xia, Z., Yuen, V. G., & McNeill, J. H. (2007) · Metabolism · doi:10.1016/j.metabol.2007.05.005 · Cited by 76 · see citing works
Circulating adiponectin and adiponectin receptor expression in skeletal muscle: effects of exercise
Vu, V. P., Riddell, M. C., & Sweeney, G. (2007) · Diabetes/Metabolism Research and Reviews · doi:10.1002/dmrr.778 · Cited by 63 · see citing works
Self-citationChanges of skeletal muscle adiponectin content in diet-induced insulin resistant rats.
Yang, B.-C., Chen, L.-H., Qian, Y., Triantafillou, J., McNulty, J. A., Carrick, K., Clifton, L. G., Han, B., Geske, R., Strum, J., et al. (2006) · Biochemical and Biophysical Research Communications - BBRC · doi:10.1016/j.bbrc.2005.12.172 · Cited by 49 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Li, C., Yu, K., Shyh‐Chang, N., Li, G., Jiang, L., Yu, S., Xu, L., Liu, R., Guo, Z., Xie, H., et al. (2019) · Journal of Cachexia, Sarcopenia and Muscle · doi:10.1002/jcsm.12417 · Cited by 280 · see citing works
Adiponectin, driver or passenger on the road to insulin sensitivity?
Ye, R., & Scherer, P. E. (2013) · Molecular Metabolism · doi:10.1016/j.molmet.2013.04.001 · Cited by 251 · see citing works
Shortreed, K. E., Krause, M. P., Huang, J. H., Dhanani, D., Moradi, J., Ceddia, R. B., & Hawke, T. J. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007293 · Cited by 131 · see citing works
Self-citationFoucault, A., Mathé, V., Lafont, R., Even, P., Dioh, W., Veillet, S., Tomé, D., Huneau, J., Hermier, D., & Quignard‐Boulangé, A. (2012) · Obesity · doi:10.1038/oby.2011.257 · Cited by 122 · see citing works
Adiponectin—Consideration for its Role in Skeletal Muscle Health
Krause, M. P., Milne, K. J., & Hawke, T. J. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20071528 · Cited by 121 · see citing works
Self-citationAdiponectin resistance in skeletal muscle: Pathophysiological implications in chronic heart failure
Sente, T., Van Berendoncks, A. M., Hoymans, V. Y., & Vrints, C. J. (2016) · Journal of Cachexia, Sarcopenia and Muscle · doi:10.1002/jcsm.12086 · Cited by 95 · see citing works
Abou-Samra, M., Selvais, C. M., Dubuisson, N., & Brichard, S. M. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21072620 · Cited by 72 · see citing works
Adiponectin as a tissue regenerating hormone: More than a metabolic function
Fiaschi, T., Magherini, F., Gamberi, T., Modesti, P. A., & Modesti, A. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1537-4 · Cited by 66 · see citing works
Involvement of adiponectin in the pathogenesis of dystrophinopathy
Abou-Samra, M., Lecompte, S., Schakman, O., Noel, L., Many, M. C., Gailly, P., & Brichard, S. M. (2015) · Skeletal Muscle · doi:10.1186/s13395-015-0051-9 · Cited by 55 · see citing works
Adiponectin and Skeletal Muscle
Jortay, J., Senou, M., Abou-Samra, M., Noel, L., Robert, A., Many, M.-C., & Brichard, S. M. (2012) · The American Journal of Pathology · doi:10.1016/j.ajpath.2012.03.035 · Cited by 52 · see citing works
Quinn, L., Anderson, Conner, Pistilli, & Wolden-Hanson (2011) · International Journal of Interferon Cytokine and Mediator Research · doi:10.2147/ijicmr.s19007 · Cited by 47 · see citing works
Local induction of adiponectin reduces lipopolysaccharide-triggered skeletal muscle damage
Jortay, J., Senou, M., Delaigle, A., Noel, L., Funahashi, T., Maeda, N., Many, M. C., & Brichard, S. M. (2010) · Endocrinology · doi:10.1210/en.2009-1462 · Cited by 44 · see citing works
Sente, T., Van Berendoncks, A. M., Fransen, E., Vrints, C. J., & Hoymans, V. Y. (2016) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00831.2015 · Cited by 41 · see citing works
Role of Adipokines in Sarcopenia
Lu, W., Feng, W., Lai, J., Yuan, D., Xiao, W., & Li, Y. (2023) · Chinese Medical Journal · doi:10.1097/cm9.0000000000002255 · Cited by 34 · see citing works
Potential Therapeutic Action of Adiponectin in Duchenne Muscular Dystrophy
Abou-Samra, M., Boursereau, R., Lecompte, S., Noel, L., & Brichard, S. M. (2017) · The American Journal of Pathology · doi:10.1016/j.ajpath.2017.02.018 · Cited by 24 · see citing works
- Authors
- Krause, M. P., Liu, Y., Vu, V., Chan, L., Xu, A., Riddell, M. C., Sweeney, G., & Hawke, T. J.
- Affiliations
- York University (CA)
- ORCID
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2008-07
- Volume
- 295
- Issue
- 1
- Pages
- C203-C212
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- PMC
- PMC2493546
- OpenAlex
- W2072137305
- Cited by
- 168
- References
- 33
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 4 · 2023: 8 · 2022: 13 · 2021: 13 · 2020: 10 · 2019: 9 · 2018: 6 · 2017: 8 · 2016: 9 · 2015: 12 · 2014: 7 · 2013: 11 · 2012: 17
- FWCI
- 3.97
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- adiponectin, skeletal muscle, intramyocellular lipid, muscle fiber type, type IIA fibers, type IIb muscle fibers, muscle contractility, adiponectin knockout mice, muscle hypertrophy, epididymal adipose tissue, muscle fatigue, L6 myotubes
- MeSH terms
- Animals, Cell Line, Phenotype, Mice, Knockout, Muscle, Skeletal, Muscle Fibers, Skeletal, Mice, Adiponectin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
