Cited by 263Iron Metabolism and DisordersNeurological diseases and metabolismFerroptosis and cancer prognosis

Release of iron from ferritin requires lysosomal activity

Kidane, Theodros Z., Sauble, Eric, Linder, Maria C.·2006·American Journal of Physiology-Cell Physiology

Abstract

How ferritin-Fe becomes available for cell functions is unknown. Our previous studies with rat hepatoma cells indicated ferritin had to be degraded to release its Fe. In these studies, we investigated whether this occurs in other cell types and whether lysosomes are required. Release of ferritin-Fe was induced with desferoxamine (DFO) in (59)Fe-preloaded hepatoma, Caco2, and erythroid K562 cells and measured by rocket immunoelectrophoresis and autoradiography. The half-lives for ferritin-(59)Fe and protein were parallel (23, 16, and 11 h for the hepatic, Caco2, and K562 cells, respectively). Co-treatment with 180 microM Fe, leupeptin, chymostatin, or chloroquine markedly decreased rates of ferritin-Fe release and ferritin degradation. Lactacystin had no effect except for a small one in erythroid cells. Fractionation of hepatoma cell lysates on iodixanol gradients showed rapid depletion of cytosolic ferritin by DFO treatment but no accumulation in lysosomes. We conclude that regardless of cell type, release of Fe from ferritin occurs mainly through lysosomal proteolysis.

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Crossref · 10.1152/ajpcell.00505.2005

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Authors
Kidane, T. Z., Sauble, E., & Linder, M. C.
Affiliations
California State University, Fullerton (US)
Venue
American Journal of Physiology-Cell Physiology
Published
2006-09
Volume
291
Issue
3
Pages
C445-C455
ISSN
  • 0363-6143 (ISSN-L)
  • 1522-1563
OpenAlex
W2136891799
Cited by
263
References
51
Citations by year
2026: 6 · 2025: 13 · 2024: 17 · 2023: 13 · 2022: 12 · 2021: 17 · 2020: 13 · 2019: 10 · 2018: 16 · 2017: 16 · 2016: 12 · 2015: 12 · 2014: 17 · 2013: 13 · 2012: 9
FWCI
7.53
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Cell Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01dk053080-04)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK-53080)
Topics
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Ferroptosis and cancer prognosis
Keywords
ferritin, iron release, lysosomal proteolysis, deferoxamine, ferritin degradation, lysosomes, hepatoma cells, Caco-2 cells, iron metabolism, leupeptin, chymostatin, chloroquine, lactacystin
MeSH terms
Animals, Chloroquine, Deferoxamine, Ferritins, Humans, Immunoelectrophoresis, Iron, Leupeptins, Lysosomes, Oligopeptides, Peptide Hydrolases, Protease Inhibitors, Caco-2 Cells, K562 Cells, Enterocytes, Hepatocytes, Erythroid Cells, Rats

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