Cited by 108Dietary Effects on HealthMuscle metabolism and nutritionAdipose Tissue and Metabolism

Fasting for 72 h increases intramyocellular lipid content in nondiabetic, physically fit men

Stannard, S. R., Thompson, M. W., Fairbairn, K., Huard, B., Sachinwalla, T., Thompson, C. H.·2002·American Journal of Physiology-Endocrinology and Metabolism

Abstract

The purpose of this study was to determine changes in intramyocellular lipid (IMCL) content in the vastus lateralis of nondiabetic, physically fit males over 72 h of fasting. Six men, mean age 35 yr (range 23–55 yr), body mass index 23.7 kg/m 2(21.2–27.4 kg/m 2), undertook a water-only fast for 84 h. Vastus lateralis IMCL content was determined using proton magnetic resonance spectroscopy after 12 and 84 h of fasting. Venous blood was sampled at 12-h intervals throughout the fast. IMCL-(CH 2)n/water and IMCL-(CH 2)n/total creatine ratios increased from 0.00623 ± 0.00065 to 0.0142 ± 0.0015 ( P = 0.002) and 6.82 ± 0.87 to 14.96 ± 1.73 ( P = 0.001), respectively. Plasma free fatty acid (FFA), serum triglyceride, and whole blood 3-hydroxybutyrate concentrations increased ( P < 0.001, <0.05, <0.03, respectively), whereas plasma glucose and serum insulin concentrations decreased (both P < 0.001) during fasting. In conclusion, 72-h water-only fasting produces a large increase in plasma FFA concentration, a drop in serum insulin concentration, and accumulation of IMCL in the vastus lateralis muscle of nondiabetic, physically fit men.

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Crossref · 10.1152/ajpendo.00108.2002

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Authors
Stannard, S. R., Thompson, M. W., Fairbairn, K., Huard, B., Sachinwalla, T., & Thompson, C. H.
Affiliations
  • The University of Sydney (AU)
  • Rayscan Imaging, Liverpool 2170, New South Wales, Australia
Venue
American Journal of Physiology-Endocrinology and Metabolism
Published
2002-12-01
Volume
283
Issue
6
Pages
E1185-E1191
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
OpenAlex
W2115449275
Cited by
108
References
48
Citations by year
2026: 6 · 2024: 2 · 2023: 9 · 2022: 2 · 2021: 3 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 4
FWCI
3.60
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Dietary Effects on Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
water-only fasting, intramyocellular lipid, vastus lateralis, plasma free fatty acids, insulin, ketone bodies, beta-hydroxybutyrate, serum triglycerides, muscle lipid accumulation, caloric restriction, physical fitness
MeSH terms
Adult, Blood Glucose, Fasting, Fatty Acids, Nonesterified, Humans, Insulin, Intracellular Fluid, Leg, Magnetic Resonance Imaging, Male, Middle Aged, Magnetic Resonance Spectroscopy, Physical Fitness, Reference Values, Time Factors, Triglycerides, Muscle, Skeletal, 3-Hydroxybutyric Acid, Lipid Metabolism

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