Analyte flux through chronically implanted subcutaneous polyamide membranes differs in humans and rats
Abstract
The rat is commonly used to evaluate physiological responses of subcutaneous tissue to implanted devices. In vivo longevity of various devices and the biocompatibility of biomaterials depend on how adjacent tissue interacts. How closely the rat model predicts the human response has not been well characterized. The objective of this study was to compare rat and human subcutaneous foreign body responses by monitoring the biochemical environment at a polymer-tissue interface over 8 days using microdialysis. Polyamide microdialysis probes were implanted subcutaneously in humans and rats ( n = 12). Daily microdialysis samples were analyzed for glucose, lactate, pyruvate, glycerol, and urea. Blood glucose was also monitored. Analyte concentrations differed significantly between rats and humans at the implant-tissue interface. There were also qualitative differences in the 8-day trends. For example, over 8 days, microdialysate glucose increased two- to fourfold in humans but decreased in rats ( P < 0.001). This study reveals profound physiological differences at material-tissue interfaces in rats and humans and highlights the need for caution when extrapolating subcutaneous rat biocompatibility data to humans.
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The ratio of dialysate glucose to blood glucose was significantly different between rats and humans, indicating differences in relative glucose availability in tissue surrounding an implanted device.
“the ratio of the glucose concentration in the dialysate to that in blood were significantly different between rats and humans indicating differences in the relative glucose availability in the tissue surrounding an implanted device.126”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [126]
Dialysate concentrations of glucose, pyruvate, lactate, glycerol, and urea at a microdialysis probe-tissue interface were significantly different between rats and humans throughout the first 6 days, except for glycerol on day 0.
“The dialysate concentrations were significantly different for all metabolites in rats and humans throughout the first 6 days of the experiment, with the exception of glycerol on day 0.126”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [126]
Human subcutaneous tissue had a high concentration of adipose cells, whereas rat tissue had larger amounts of collagen.
“Histology samplings from human subcutaneous tissue showed a high concentration of adipose cells while rat tissue was characterized with larger amounts of collagen.126”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [126]
These results require caution when extrapolating results from animal models to humans.
“Taken together, these results necessitate careful consideration and caution when extrapolating results from animal models to humans.”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [126]
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- Authors
- Wisniewski, N., Rajamand, N., Adamsson, U., Lins, P. E., Reichert, W. M., Klitzman, B., & Ungerstedt, U.
- Affiliations
- Departments of Biomedical Engineering and
- Department of Physiology and Pharmacology, Karolinska Institute, Stockholm S-17177; and
- Danderyds sjukhus (SE)
- Duke University (US)
- Karolinska Institutet (SE)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2002-06-01
- Volume
- 282
- Issue
- 6
- Pages
- E1316-E1323
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2161380946
- Cited by
- 50
- References
- 25
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 3
- FWCI
- 1.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK54932)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Health Sciences › Medicine › Surgery › Surgical Sutures and Adhesives
- Keywords
- polyamide membranes, biocompatibility, human-rat comparison, microdialysis, implant-tissue interface, glucose, lactate, pyruvate, glycerol, urea, blood glucose, biomaterials
- MeSH terms
- Adult, Animals, Biocompatible Materials, Blood Glucose, Glucose, Glycerol, Humans, Male, Membranes, Artificial, Microscopy, Electron, Nylons, Species Specificity, Time Factors, Urea, Rats, Sprague-Dawley, Microdialysis, Pyruvic Acid, Lactic Acid, Prostheses and Implants, Rats
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