Ursolic acid stimulates mTORC1 signaling after resistance exercise in rat skeletal muscle
Abstract
A recent study identified ursolic acid (UA) as a potent stimulator of muscle protein anabolism via PI3K/Akt signaling, thereby suggesting that UA can increase Akt-independent mTOR complex 1 (mTORC1) activation induced by resistance exercise via Akt signaling. The purpose of the present study was to investigate the effect of UA on resistance exercise-induced mTORC1 activation. The right gastrocnemius muscle of male Sprague-Dawley rats aged 11 wk was isometrically exercised via percutaneous electrical stimulation (stimulating ten 3-s contractions per set for 5 sets), while the left gastrocnemius muscle served as the control. UA or placebo (PLA; corn oil only) was injected intraperitoneally immediately after exercise. The rats were killed 1 or 6 h after the completion of exercise and the target tissues removed immediately. With placebo injection, the phosphorylation of p70(S6K) at Thr(389) increased 1 h after resistance exercise but attenuated to the control levels 6 h after the exercise. On the other hand, the augmented phosphorylation of p70(S6K) was maintained even 6 h after exercise when UA was injected immediately after exercise. A similar trend of prolonged phosphorylation was observed in PRAS40 Thr(246), whereas UA alone or resistance exercise alone did not alter its phosphorylation level at 6 h after intervention. These results indicate that UA is able to sustain resistance exercise-induced mTORC1 activity.
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UA administration enhanced S6K1 Thr389 phosphorylation 6 hours following a single bout of resistance exercise in rats.
“UA administration enhanced S6K1 Thr389 phosphorylation 6 hours following a single bout of resistance exercise in rats [188]”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [188]
UA might also enhance mTORC1 activity in skeletal muscle.
“indicating that UA might also enhance mTORC1 activity in skeletal muscle.”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [188]
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- Authors
- Ogasawara, R., Sato, K., Higashida, K., Nakazato, K., & Fujita, S.
- Affiliations
- Ritsumeikan University (JP)
- Nippon Sport Science University (JP)
- Corresponding author
- Ogasawara, Riki
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2013-09-15
- Volume
- 305
- Issue
- 6
- Pages
- E760-E765
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2086744127
- Cited by
- 70
- References
- 29
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 2 · 2023: 6 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 4 · 2018: 5 · 2017: 7 · 2016: 12 · 2015: 5 · 2014: 3 · 2013: 1
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (25560378, 23700849)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- ursolic acid, mTORC1 signaling, resistance training, skeletal muscle, muscle protein anabolism, PRAS40 phosphorylation, PI3K/Akt signaling, gastrocnemius
- MeSH terms
- Mechanistic Target of Rapamycin Complex 1, Ursolic Acid, Animals, Isometric Contraction, Male, Phosphorylation, Physical Conditioning, Animal, Triterpenes, Signal Transduction, Rats, Sprague-Dawley, Muscle, Skeletal, Multiprotein Complexes, Proto-Oncogene Proteins c-akt, Rats, TOR Serine-Threonine Kinases
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