Sleep Deprivation Increases Mortality in Female Mice Bearing Ehrlich Ascitic Tumor
Abstract
Objectives: Sleep deprivation is a growing public health hazard, yet it is still under-recognized. Sleep disorders and disruption of sleep patterns may compromise the immune function and adversely affect host resistance to infectious diseases. This is a particular risk in cancer patients, who report a high frequency of sleep disturbances. The present study investigated the effects of sleep deprivation on the development of Ehrlich ascitic tumors (EAT) in female BALB/c mice. Our study also evaluated whether EAT would induce alterations in sleep pattern. Spleen lymphocyte cell populations and mortality were also quantified. Methods: Female BALB/c mice were intraperitoneally inoculated with EAT cells. Immediately after the inoculation procedure, animals were sleep deprived for 72 h. Ten or 15 days after inoculation, the number of tumoral cells was quantified and the lymphocytic cell population in the spleen was characterized by flow cytometry. In addition, the effect of sleep deprivation on EAT-induced mortality was quantified and the influence of EAT on sleep patterns was determined. Results: Sleep deprivation did not potentiate EAT growth, but it significantly increased mortality. Additionally, both EAT and sleep deprivation decreased frequencies of splenic CD4+, CD8+ and CD19+ cells. With respect to sleep patterns, EAT significantly enhanced paradoxical sleep time. Conclusions: Although sleep deprivation did not potentiate EAT growth, it decreased the survival of female tumor-bearing mice.
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The most consistent findings on sleep effects on leukocyte distribution are for B cells and NK cells, which increase in numbers in the murine spleen after normal sleep.
“The most consistent findings with respect to the effect of sleep on leukocyte distribution have been observed for B cells and NK cells, which were shown to be increased in numbers in the murine spleen after normal sleep (137, 346, 360, 597).”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [360]
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The Sleep-Immune Crosstalk in Health and Disease
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- Authors
- Maragno-Correa, J. M. R., Patti, C. L., Zanin, K. A., Wuo-Silva, R., Ruiz, F. S., Zager, A., Sá-Nunes, A., Tufik, S., Andersen, M. L., & Frussa-Filho, R.
- Affiliations
- Universidade Federal de São Paulo (BR)
- Venue
- Neuroimmunomodulation
- Publisher
- S. Karger AG
- Published
- 2013
- Volume
- 20
- Issue
- 3
- Pages
- 134-140
- ISSN
- 1021-7401 (ISSN-L)
- 1423-0216
- OpenAlex
- W2025007657
- Cited by
- 26
- References
- 42
- Citations by year
- 2026: 3 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 3 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 7
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- NeuroImmunoModulation (publishedVersion)
- PubMed (publishedVersion)
- UNIFESP Institutional Repository (Universidade Federal de São Paulo) (submittedVersion)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Cancer, Stress, Anesthesia, and Immune Response
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Keywords
- sleep deprivation, Ehrlich ascites carcinoma, female mice, CD4+ T cells, CD8+ T cells, B cells, REM sleep, sleep patterns, tumor growth, tumor immunology
- MeSH terms
- Analysis of Variance, Animals, Carcinoma, Ehrlich Tumor, Disease Models, Animal, Female, Flow Cytometry, Humans, Lymphocytes, Mice, Inbred BALB C, Neoplasm Transplantation, Sleep Deprivation, Spleen, Antigens, CD, Mice
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