Effects of Sleep Deprivation on Mice Bone Marrow and Spleen B Lymphopoiesis
Abstract
B lymphocytes are immune cells crucial for the maintenance and viability of the humoral response. Sleep is an essential event for the maintenance and integrity of all systems, including the immune system (IS). Thus, sleep deprivation (SD) causes problems in metabolism and homeostasis in many cell systems, including the IS. In this study, our goal was to determine changes in B lymphocytes from the bone marrow (BM) and spleen after SD. Three‐month‐old male Swiss mice were used. These mice were sleep deprived through the modified multiple platform method for different periods (24, 48, and 72 h), whereas another group was allowed to sleep for 24 h after 72 h of SD (rebound group) and a third group was allowed to sleep normally during the entire experiment. After this, the spleen and BM were collected, and cell analyses were performed. The numbers of B lymphocytes in the BM and spleen were reduced by SD. Additionally, reductions in the percentage of lymphocyte progenitors and their ability to form colonies were observed. Moreover, an increase in the death of B lymphocytes from the BM and spleen was associated with an increase in oxidative stress indicators, such as DCFH‐DA, CAT, and mitochondrial SOD. Rebound was not able to reverse most of the alterations elicited by SD. The reduction in B lymphocytes and their progenitors by cell death, with a concomitant increase in oxidative stress, showed that SD promoted a failure in B lymphopoiesis. J. Cell. Physiol. 231: 1313–1320, 2016. © 2015 Wiley Periodicals, Inc.
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The most consistent findings on sleep effects on leukocyte distribution are for B cells and NK cells, which increase in numbers in the murine spleen after normal sleep.
“The most consistent findings with respect to the effect of sleep on leukocyte distribution have been observed for B cells and NK cells, which were shown to be increased in numbers in the murine spleen after normal sleep (137, 346, 360, 597).”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [346]
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- Authors
- Lungato, L., Nogueira‐Pedro, A., Carvalho Dias, C., Paredes‐Gamero, E. J., Tufik, S., & D'Almeida, V.
- Affiliations
- Universidade Federal de São Paulo (BR)
- Department de Biochemistry Universidade Federal de São Paulo, UNIFESP São Paulo SP Brazil
- Department of Psychobiology Universidade Federal de São Paulo, UNIFESP São Paulo SP Brazil
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 2015-11-20
- Volume
- 231
- Issue
- 6
- Pages
- 1313-1320
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2196839166
- Cited by
- 18
- References
- 45
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 3 · 2023: 1 · 2022: 1 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 1
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cellular Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- FAPESP/CEPID (98/14303-3)
- Topics
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Keywords
- sleep deprivation, B cell development, B cells, bone marrow, spleen, oxidative stress, apoptosis, immune system, sleep rebound, humoral immunity
- MeSH terms
- Animals, B-Lymphocytes, Bone Marrow, Disease Models, Animal, Male, Sleep Deprivation, Spleen, Time Factors, Biomarkers, Cell Death, Oxidative Stress, Lymphocyte Count, Lymphopoiesis, Mice, Precursor Cells, B-Lymphoid
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