Effects of acute and chronic sleep loss on immune modulation of rats
Open accessAbstract
Sleep deprivation is now recognized as an increasingly common condition inherent to modern society, and one that in many ways, is detrimental to certain physiological systems, namely, immune function. Although sleep is now viewed by a significant body of researchers as being essential for the proper working of a host of defense systems, the consequences of a lack of sleep on immune function remains to be fully comprehended. The aim of the current study was to investigate how paradoxical sleep deprivation (PSD) for 24 and 96 h and sleep restriction (SR) for 21 days by the modified multiple-platform method, and their respective 24-h recovery periods, affect immune activation in rats. To this end, we assessed circulating white blood cell counts, lymphocyte count within immune organs, as well as Ig and complement production. The data revealed that PSD for 96 h increased complement C3 and corticosterone concentration in relation to the control group. In contrast, the spleen weight, total leukocytes, and lymphocytes decreased during SR for 21 days when compared with the control group, although production of a certain class of immunoglobulin, the IgM, did increase. After recovery sleep, lymphocyte count in axillary lymph nodes grew when rats had rebound sleep after PSD for 24 h, neutrophils increased after PSD 96 h and lymphocytes numbers were higher after SR 21 days. Such alterations during sleep deprivation suggest only minor alterations of nonspecific immune parameters during acute PSD, and a significant impairment in cellular response during chronic SR.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In rats, lymphocyte counts in lymph nodes indeed increased after recovery sleep that followed a period of sleep deprivation.
“In rats, lymphocyte counts in lymph nodes indeed increased after recovery sleep that followed a period of sleep deprivation (1354).”
From About Sleep's Role in Memory (2013) · cites this paper as [1354]
Inhibition of prostaglandin production by COX-2 inhibitors reduced spontaneous and TNF-induced increases in NREM sleep in the animal model.
“Inhibition of PG production by COX-2 inhibitors reduced spontaneous and TNF-induced increases in NREM sleep in the animal model (538, 595)”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [595]
Cites
Kleitman, N., & Eckenhoff, J. E. (1964) · The American Journal of the Medical Sciences · doi:10.1097/00000441-196502000-00024 · Cited by 1,079 · see citing works
Effects of sleep and circadian rhythm on human circulating immune cells
Born, J., Lange, T., Hansen, K., Mölle, M., & Fehm, H. L. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.158.9.4454 · Cited by 611 · see citing works
Sick and tired: does sleep have a vital role in the immune system?
Bryant, P. A., Trinder, J., & Curtis, N. (2004) · Nature Reviews Immunology · doi:10.1038/nri1369 · Cited by 551 · see citing works
Dhabhar, F. S. (2002) · Brain Behavior and Immunity · doi:10.1016/s0889-1591(02)00036-3 · Cited by 464 · see citing works
Machado, R. B., Hipólide, D. C., Benedito‐Silva, A. A., & Tufik, S. (2004) · Brain Research · doi:10.1016/j.brainres.2004.01.019 · Cited by 424 · see citing works
Self-citationStress-induced enhancement of antigen-specific cell-mediated immunity
Dhabhar, F. S., & McEwen, B. S. (1996) · The Journal of Immunology · doi:10.4049/jimmunol.156.7.2608 · Cited by 419 · see citing works
Dhabhar, F. S. (2000) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2000.tb05454.x · Cited by 279 · see citing works
Sustained sleep deprivation impairs host defense
Everson, C. A. (1993) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1993.265.5.r1148 · Cited by 251 · see citing works
Andersen, M. L., Martins, P. J. F., D’Almeida, V., Bignotto, M., & Tufik, S. (2005) · Journal of Sleep Research · doi:10.1111/j.1365-2869.2004.00428.x · Cited by 246 · see citing works
Self-citationStress, Leukocyte Trafficking, and the Augmentation of Skin Immune Function
Dhabhar, F. S. (2003) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2003.tb03151.x · Cited by 166 · see citing works
Immune Correlates of Depression
Irwin, M. R. (1999) · Advances in experimental medicine and biology · doi:10.1007/978-0-585-37970-8_1 · Cited by 162 · see citing works
Bøyum, A., Wiik, P., Gustavsson, E., Veiby, O. P., Reseland, J. E., Haugen, A.-H., & Opstad, P. K. (1996) · Scandinavian Journal of Immunology · doi:10.1046/j.1365-3083.1996.d01-32.x · Cited by 153 · see citing works
Sleep as a Prognostic Indicator During Infectious Disease in Rabbits
Toth, L. A., Tolley, E. A., & Krueger, J. M. (1993) · Experimental Biology and Medicine · doi:10.3181/00379727-203-43590 · Cited by 153 · see citing works
Different stress modalities result in distinct steroid hormone responses by male rats
Andersen, M. L., Bignotto, M., Machado, R. B., & Tufik, S. (2004) · Brazilian Journal of Medical and Biological Research · doi:10.1590/s0100-879x2004000600003 · Cited by 151 · see citing works
Self-citationEverson, C. A. (2005) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00021.2005 · Cited by 141 · see citing works
Darko, D. F., Miller, J. C., Gallen, C., White, J., Koziol, J., Brown, S. J., Hayduk, R., Atkinson, J. H., Assmus, J., & Munnell, D. T. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.26.12080 · Cited by 121 · see citing works
Irwin, M. R., Smith, T. L., & Gillin, J. C. (1992) · Psychosomatic Medicine · doi:10.1097/00006842-199201000-00002 · Cited by 114 · see citing works
White blood cells and cortisol after sleep deprivation and recovery sleep in humans
Heiser, P., Dickhaus, B., Schreiber, W., Clement, H. W., Hasse, C., Hennig, J., Remschmidt, H., Krieg, J. C., Wesemann, W., & Opper, C. (2000) · European Archives of Psychiatry and Clinical Neurosciences · doi:10.1007/pl00007534 · Cited by 105 · see citing works
Effects of sleep and sleep deprivation on immunoglobulins and complement in humans
Liu, H., Hua, F., Dian-dong, H., & Hong, Y. (2006) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2006.09.005 · Cited by 90 · see citing works
Effects of 48 hours sleep deprivation on human immune profile.
Öztürk, L., Pelin, Z., Karadeniz, D., Kaynak, H., Cakar, L., & Gözükırmızı, E. (1999) · PubMed · Cited by 86 · see citing works
Engeland, C. G., Nielsen, D. V., Kavaliers, M., & Ossenkopp, K. (2001) · Physiology & Behavior · doi:10.1016/s0031-9384(00)00436-4 · Cited by 79 · see citing works
Machado, R. B., Suchecki, D., & Tufik, S. (2005) · Behavioural Brain Research · doi:10.1016/j.bbr.2005.01.001 · Cited by 79 · see citing works
Self-citationPalma, B. D., Gabriel, A., Colugnati, F. A. B., & Tufik, S. (2006) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00186.2006 · Cited by 74 · see citing works
Self-citationLentiviral infection, immune response peptides and sleep
Darko, D. F., Mitler, M. M., & Henriksen, S. J. (1995) · Advances in Neuroimmunology · doi:10.1016/0960-5428(94)00044-o · Cited by 69 · see citing works
Sleep as a host defense: Its regulation by microbial products and cytokines
Krueger, J. M., & Majde, J. A. (1990) · Clinical Immunology and Immunopathology · doi:10.1016/0090-1229(90)90033-m · Cited by 65 · see citing works
Stress-Induced Modulation of Anti-viral Immunity
Sheridan, J. F. (1998) · Brain Behavior and Immunity · doi:10.1006/brbi.1998.0521 · Cited by 53 · see citing works
Ruiz, F. S., Andersen, M. L., Zager, A., Martins, R. C. S., & Tufik, S. (2007) · Brazilian Journal of Medical and Biological Research · doi:10.1590/s0100-879x2006005000086 · Cited by 51 · see citing works
Self-citationSleep Deprivation and Sleepiness Caused by Sleep Loss
Leibowitz, S. M., Lopes, M. C., Andersen, M. L., & Kushida, C. A. (2006) · Sleep Medicine Clinics · doi:10.1016/j.jsmc.2005.11.010 · Cited by 35 · see citing works
Self-citationRuiz, F. S., Andersen, M. L., Zager, A., et al. (2007) · Brazilian Journal of Medical and Biological Research · doi:10.1590/s0100-879x2007000500005 · Cited by 34 · see citing works
Self-citationSleep as a neuroimmune phenomenon: A brief historical perspective
Krueger, J. M., & Karnovsky, M. L. (1995) · Advances in Neuroimmunology · doi:10.1016/0960-5428(94)00047-r · Cited by 29 · see citing works
Effect of Sleep Deprivation on Serum Influenza-Specific IgG
Renegar, K. B., Floyd, R. A., & Krueger, J. M. (1998) · SLEEP · doi:10.1093/sleep/21.1.19 · Cited by 26 · see citing works
Maes, M. H. J., Vandoolaeghe, E., Ranjan, R., Bosmans, E., Gastel, A. V., Bergmans, R., & Desnyder, R. (1996) · Biological Psychiatry · doi:10.1016/0006-3223(95)00627-3 · Cited by 24 · see citing works
Zalcman, S., Henderson, N., Richter, M., et al. (1991) · Behavioral Neuroscience · doi:10.1037/0735-7044.105.5.669 · Cited by 13 · see citing works
Zalcman, S., Henderson, N. M., Richter, M., et al. (1991) · Behavioral Neuroscience · doi:10.1037//0735-7044.105.5.669 · Cited by 12 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Rasch, B., & Born, J. (2013) · Physiological Reviews · doi:10.1152/physrev.00032.2012 · Cited by 2,783 · see citing works
The Sleep-Immune Crosstalk in Health and Disease
Besedovsky, L., Lange, T., & Haack, M. (2019) · Physiological Reviews · doi:10.1152/physrev.00010.2018 · Cited by 1,479 · see citing works
Besedovsky, L., Lange, T., & Born, J. (2012) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-011-1044-0 · Cited by 1,153 · see citing works
Besedovsky, L., Ngo, H.-V. V., Dimitrov, S., Gassenmaier, C., Lehmann, R., & Born, J. (2017) · Nature Communications · doi:10.1038/s41467-017-02170-3 · Cited by 144 · see citing works
Ruiz, F. S., Andersen, M. L., Martins, R. C., Zager, A., Lopes, J. D., & Tufik, S. (2012) · Innate Immunity · doi:10.1177/1753425910385962 · Cited by 132 · see citing works
Self-citationSleep Loss and Cytokines Levels in an Experimental Model of Psoriasis
Hirotsu, C., Rydlewski, M., Araújo, M. S., Tufik, S., & Andersen, M. L. (2012) · PLoS ONE · doi:10.1371/journal.pone.0051183 · Cited by 103 · see citing works
Self-citationAho, V., Ollila, H. M., Rantanen, V., Kronholm, E., Surakka, I., van Leeuwen, W. M. A., Lehto, M., Matikainen, S., Ripatti, S., Härmä, M., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0077184 · Cited by 93 · see citing works
Sleep-deprivation reduces NK cell number and function mediated by β-adrenergic signalling
De Lorenzo, B. H. P., de Oliveira Marchioro, L., Greco, C. R., & Suchecki, D. (2015) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2015.04.006 · Cited by 85 · see citing works
Neuropeptidergic control of sleep and wakefulness
Richter, C., Woods, I. G., & Schier, A. F. (2014) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-062111-150447 · Cited by 75 · see citing works
Sleep impairment during pregnancy: possible implications on mother-infant relationship
Pires, G. N., Andersen, M. L., Giovenardi, M., & Tufik, S. (2010) · Medical Hypotheses · doi:10.1016/j.mehy.2010.07.036 · Cited by 77 · see citing works
Self-citationSleep Deprivation After Septic Insult Increases Mortality Independent of Age
Friese, R. S., Bruns, B., & Sinton, C. M. (2009) · Journal of Trauma: Injury, Infection & Critical Care · doi:10.1097/ta.0b013e318190c3a1 · Cited by 64 · see citing works
Neuroscience-driven discovery and development of sleep therapeutics
Dresler, M., Spoormaker, V. I., Beitinger, P., Czisch, M., Kimura, M., Steiger, A., & Holsboer, F. (2014) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2013.10.012 · Cited by 63 · see citing works
The impact of sleep deprivation in military surgical teams: a systematic review
Parker, R. S., & Parker, P. (2017) · Journal of the Royal Army Medical Corps · doi:10.1136/jramc-2016-000640 · Cited by 47 · see citing works
Everson, C. A., Thalacker, C. D., & Hogg, N. (2008) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.90623.2008 · Cited by 35 · see citing works
Nocturnal sleep uniformly reduces numbers of different T-cell subsets in the blood of healthy men
Besedovsky, L., Dimitrov, S., Born, J., & Lange, T. (2016) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00149.2016 · Cited by 32 · see citing works
Besedovsky, L., Linz, B., Born, J., & Lange, T. (2014) · European Journal of Immunology · doi:10.1002/eji.201344265 · Cited by 31 · see citing works
Sleep Deprivation Induces Changes in Immunity in <i>Trichinella spiralis</i> -Infected Rats
Ibarra-Coronado, E. G., Velazquéz-Moctezuma, J., Diaz, D., Becerril-Villanueva, L. E., Pavón, L., & Morales-Montor, J. (2015) · International Journal of Biological Sciences · doi:10.7150/ijbs.11907 · Cited by 30 · see citing works
Sleep Deprivation Increases Mortality in Female Mice Bearing Ehrlich Ascitic Tumor
Maragno-Correa, J. M. R., Patti, C. L., Zanin, K. A., Wuo-Silva, R., Ruiz, F. S., Zager, A., Sá-Nunes, A., Tufik, S., Andersen, M. L., & Frussa-Filho, R. (2013) · Neuroimmunomodulation · doi:10.1159/000346201 · Cited by 26 · see citing works
Self-citationZager, A., Ruiz, F. S., Tufik, S., & Andersen, M. L. (2012) · Neuroimmunomodulation · doi:10.1159/000329484 · Cited by 24 · see citing works
Self-citationBesedovsky, L., Born, J., & Lange, T. (2012) · Brain, Behavior, and Immunity · doi:10.1016/j.bbi.2012.07.016 · Cited by 23 · see citing works
Paradoxical sleep deprivation impairs mouse survival after infection with malaria parasites
Lungato, L., Gazarini, M. L., Paredes-Gamero, E. J., Tufik, S., & D’Almeida, V. (2015) · Malaria Journal · doi:10.1186/s12936-015-0690-7 · Cited by 21 · see citing works
Self-citationEffects of Sleep Deprivation on Mice Bone Marrow and Spleen B Lymphopoiesis
Lungato, L., Nogueira‐Pedro, A., Carvalho Dias, C., Paredes‐Gamero, E. J., Tufik, S., & D'Almeida, V. (2016) · Journal of Cellular Physiology · doi:10.1002/jcp.25231 · Cited by 18 · see citing works
Self-citationThe dual effect of paradoxical sleep deprivation on murine immune functions
Sá-Nunes, A., Bizzarro, B., Egydio, F., Barros, M. S., Sesti-Costa, R., Soares, E. M., Pina, A., Russo, M., Faccioli, L. H., Tufik, S., et al. (2016) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2015.11.013 · Cited by 11 · see citing works
Self-citation
- Authors
- Zager, A., Andersen, M. L., Ruiz, F. S., Antunes, I. B., & Tufik, S.
- Affiliations
- Universidade Federal de São Paulo (BR)
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 2007-07
- Volume
- 293
- Issue
- 1
- Pages
- R504-R509
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- OpenAlex
- W2052082083
- Cited by
- 200
- References
- 34
- Citations by year
- 2026: 10 · 2025: 10 · 2024: 5 · 2023: 15 · 2022: 9 · 2021: 3 · 2020: 8 · 2019: 9 · 2018: 10 · 2017: 13 · 2016: 14 · 2015: 15 · 2014: 13 · 2013: 13 · 2012: 16
- FWCI
- 4.82
- Type
- journal article
- Language
- en
- Open access
- green
- americanae.aecid.es
- Licence
- other-oa
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed (publishedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Keywords
- sleep deprivation, REM sleep deprivation, sleep restriction, immune function, lymphocyte count, complement C3, corticosterone, immunoglobulin M, spleen, axillary lymph nodes, neutrophils, recovery sleep, non-specific immunity, cell-mediated immunity
- MeSH terms
- Animals, Antibody Formation, Complement System Proteins, Complement C3, Complement C4, Corticosterone, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Immunity, Cellular, Immunoglobulins, Leukocyte Count, Leukocytes, Lymph Nodes, Lymphocytes, Male, Monocytes, Neutrophils, Organ Size, Sleep Deprivation, Sleep, REM, Spleen, Rats, Wistar, Lymphocyte Count, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
