The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation
Open accessAbstract
Hematopoietic stem cells (HSCs) are the basis of bone marrow transplantation and are attractive target cells for hematopoietic gene therapy, but these important clinical applications have been severely hampered by difficulties in ex vivo expansion of HSCs. In particular, the use of cord blood for adult transplantation is greatly limited by the number of HSCs. Previously we identified angiopoietin-like proteins and IGF-binding protein 2 (IGFBP2) as new hormones that, together with other factors, can expand mouse bone marrow HSCs in culture. Here, we measure the activity of multipotent human severe combined immunodeficient (SCID)–repopulating cells (SRCs) by transplantation into the nonobese diabetic SCID (NOD/SCID) mice; secondary transplantation was performed to evaluate the self-renewal potential of SRCs. A serum-free medium containing SCF, TPO, and FGF-1 or Flt3-L cannot significantly support expansion of the SRCs present in human cord blood CD133+ cells. Addition of either angiopoietin-like 5 or IGF-binding protein 2 to the cultures led to a sizable expansion of HSC numbers, as assayed by NOD/SCID transplantation. A serum-free culture containing SCF, TPO, FGF-1, angiopoietin-like 5, and IGFBP2 supports an approximately 20-fold net expansion of repopulating human cord blood HSCs, a number potentially applicable to several clinical processes including HSC transplantation.
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Cites
Angiopoietin-Related/Angiopoietin-Like Proteins Regulate Angiogenesis
Oike, Y., Yasunaga, K., & Suda, T. (2004) · International Journal of Hematology · doi:10.1532/ijh97.04034 · Cited by 160
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Frenette, P. S., Pinho, S., Lucas, D., & Scheiermann, C. (2013) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032712-095919 · Cited by 462
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Glaser, D. E., Curtis, M. B., Sariano, P. A., Rollins, Z. A., Shergill, B. S., Anand, A., Deely, A. M., Shirure, V. S., Anderson, L., Lowen, J. M., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2021.121245 · Cited by 146
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Aryal, B., Rotllan, N., Araldi, E., Ramírez, C. M., He, S., Chousterman, B. G., Fenn, A. M., Wanschel, A., Madrigal-Matute, J., Warrier, N., et al. (2016) · Nature Communications · doi:10.1038/ncomms12313 · Cited by 93
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Ganuza, M., Hall, T., Myers, J., Nevitt, C., Sánchez-Lanzas, R., Chabot, A., Ding, J., Kooienga, E., Caprio, C., Finkelstein, D., et al. (2022) · Nature Cell Biology · doi:10.1038/s41556-022-00999-5 · Cited by 83
Romeo, S., Yin, W., Kozlitina, J., Pennacchio, L. A., Boerwinkle, E., Hobbs, H. H., & Cohen, J. C. (2008) · Journal of Clinical Investigation · doi:10.1172/jci37118 · Cited by 58
Roles of angiopoietin-like proteins in regulation of stem cell activity
Kadomatsu, T., & Oike, Y. (2019) · The Journal of Biochemistry · doi:10.1093/jb/mvz005 · Cited by 23
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Pastoors, D., Havermans, M., Mulet-Lazaro, R., Brian, D., Noort, W., Grasel, J., Hoogenboezem, R., Smeenk, L., Demmers, J. A. A., Milsom, M. D., et al. (2024) · Science Advances · doi:10.1126/sciadv.adk9076 · Cited by 15
Metadata
- Authors
- Zhang, C. C., Kaba, M., Iizuka, S., Huynh, H., & Lodish, H. F.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2008-04-01
- Volume
- 111
- Issue
- 7
- Pages
- 3415-3423
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2003209323
- Cited by
- 246
- References
- 1
- FWCI
- 13.93
- Type
- journal article
- Language
- en
- Open access
- hybrid
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