Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B″ subunits of protein phosphatase 2A
Open accessAbstract
BACKGROUND: Protein phosphatase 2A (PP2A) is a serine/threonine-specific phosphatase displaying vital functions in growth and development through its role in various signalling pathways. PP2A holoenzymes comprise a core dimer composed of a catalytic C and a structural A subunit, which can associate with a variable B-type subunit. The importance of the B-type subunits for PP2A regulation cannot be overestimated as they determine holoenzyme localisation, activity and substrate specificity. Three B-type subunit families have been identified: PR55/B, PR61/B' and PR72/B", of which the latter is currently the least characterised. RESULTS: We deduced the sequences and genomic organisation of the different murine PR72/B" isoforms: three genes encode nine isoforms, five of which are abundantly expressed and give rise to genuine PP2A subunits. Thereby, one novel subunit was identified. Using Northern blotting, we examined the tissue-specific and developmental expression of these subunits. All subunits are highly expressed in heart, suggesting an important cardiac function. Immunohistochemical analysis revealed a striated expression pattern of PR72 and PR130 in heart and skeletal muscle, but not in bladder smooth muscle. The subcellular localisation and cell cycle regulatory ability of several PR72/B" isoforms were determined, demonstrating differences as well as similarities. CONCLUSION: In contrast to PR55/B and PR61/B', the PR72/B" family seems evolutionary more divergent, as only two of the murine genes have a human orthologue. We have integrated these results in a more consistent nomenclature of both human and murine PR72/B" genes and their transcripts/proteins. Our results provide a platform for the future generation of PR72/B" knockout mice.
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B55γ is nearly exclusively expressed in brain and B′′α is highly expressed in heart, consistent with previous reports.
“This is probably best illustrated for B55γ (nearly exclusively expressed in brain) and B″α (highly expressed in heart), in accordance with previously published work of others [20][21][22].”
From Physiologic functions of PP2A: Lessons from genetically modified mice (2019) · cites this paper as [22]
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- Authors
- Zwaenepoel, K., Louis, J. V., Goris, J., & Janssens, V.
- Affiliations
- KU Leuven (BE)
- Corresponding author
- Janssens, Veerle
- Venue
- BMC Genomics
- Published
- 2008-08-20
- Volume
- 9
- Issue
- 1
- Pages
- 393-393
- ISSN
- 1471-2164 (ISSN-L)
- PMC
- PMC2529318
- OpenAlex
- W2118164364
- Cited by
- 30
- References
- 27
- Citations by year
- 2025: 1 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 5 · 2014: 1 · 2013: 3 · 2012: 4
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- BMC Genomics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Lirias (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Vlaamse regering
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- protein phosphatase 2A, gene family evolution, protein isoforms, genome organization, tissue-specific gene expression, developmental regulation, subcellular localization, cell cycle regulation, skeletal muscle, nomenclature, knockout mice
- MeSH terms
- Amino Acid Sequence, Animals, Humans, Isoenzymes, Molecular Sequence Data, Myocardium, Phylogeny, RNA Probes, Sequence Alignment, Sequence Homology, Amino Acid, Alternative Splicing, Muscle, Skeletal, Gene Expression Regulation, Developmental, Cell Cycle Proteins, Computational Biology, Reverse Transcriptase Polymerase Chain Reaction, Protein Subunits, Databases, Protein, Mice, Protein Phosphatase 2
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