Characterization of the ribonuclease activity on the skin surface
Open accessAbstract
The rapid degradation of ribonucleic acids (RNA) by ubiquitous ribonucleases limits the efficacy of new therapies based on RNA molecules. Therefore, our aim was to characterize the natural ribonuclease activities on the skin and in blood plasma i.e. at sites where many drugs in development are applied. On the skin surfaces of Homo sapiens and Mus musculus we observed dominant pyrimidine-specific ribonuclease activity. This activity is not prevented by a cap structure at the 5'-end of messenger RNA (mRNA) and is not primarily of a 5'- or 3'-exonuclease type. Moreover, the ribonuclease activity on the skin or in blood plasma is not inhibited by chemical modifications introduced at the 2'OH group of cytidine or uridine residues. It is, however, inhibited by the ribonuclease inhibitor RNasin® although not by the ribonuclease inhibitor SUPERase· In™. The application of our findings in the field of medical science may result in an improved efficiency of RNA-based therapies that are currently in development.
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Naked RNA vaccines suffer from limited potency, in part due to RNA instability in vivo, related to the presence of degradative enzymes in tissues.
“However, naked RNA vaccines suffer from limited potency, in part due to RNA instability in vivo, related to the presence of degradative enzymes in tissues (15).”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [15]
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- Authors
- Probst, J., Brechtel, S., Scheel, B., Hoerr, I., Jung, G., Rammensee, H.-G., & Pascolo, S.
- Affiliations
- University of Tübingen (DE)
- CureVac (Germany) (DE)
- Corresponding author
- Pascolo, Steve
- Venue
- Genetic Vaccines and Therapy
- Published
- 2006-05-29
- Volume
- 4
- Issue
- 1
- Pages
- 4-4
- ISSN
- 1479-0556 (ISSN-L)
- PMC
- PMC1524753
- OpenAlex
- W2163659675
- Cited by
- 84
- References
- 44
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 7 · 2017: 5 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 5
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Genetic Vaccines and Therapy (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Eberhard Karls Universität Tübingen
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- ribonuclease activity, skin surface, blood plasma, RNA degradation, RNA therapeutics, 5' cap
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