Cited by 84RNA Interference and Gene DeliveryRNA Research and SplicingRNA regulation and disease

Characterization of the ribonuclease activity on the skin surface

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Probst, Jochen, Brechtel, Sonja, Scheel, Birgit, Hoerr, Ingmar, Jung, Günther, Rammensee, Hans-Georg, Pascolo, Steve·2006·Genetic Vaccines and Therapy

Abstract

The rapid degradation of ribonucleic acids (RNA) by ubiquitous ribonucleases limits the efficacy of new therapies based on RNA molecules. Therefore, our aim was to characterize the natural ribonuclease activities on the skin and in blood plasma i.e. at sites where many drugs in development are applied. On the skin surfaces of Homo sapiens and Mus musculus we observed dominant pyrimidine-specific ribonuclease activity. This activity is not prevented by a cap structure at the 5'-end of messenger RNA (mRNA) and is not primarily of a 5'- or 3'-exonuclease type. Moreover, the ribonuclease activity on the skin or in blood plasma is not inhibited by chemical modifications introduced at the 2'OH group of cytidine or uridine residues. It is, however, inhibited by the ribonuclease inhibitor RNasin® although not by the ribonuclease inhibitor SUPERase· In™. The application of our findings in the field of medical science may result in an improved efficiency of RNA-based therapies that are currently in development.

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  • Naked RNA vaccines suffer from limited potency, in part due to RNA instability in vivo, related to the presence of degradative enzymes in tissues.

    “However, naked RNA vaccines suffer from limited potency, in part due to RNA instability in vivo, related to the presence of degradative enzymes in tissues (15).”

    From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [15]

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Research works printed but not linked: 1

  1. Levy CC Karpetsky TP Holcenberg JS and Roberts J Human Ribonucleases Enzymes as drugs 1981 New York, John Wiley & Sons 103 166

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Authors
Probst, J., Brechtel, S., Scheel, B., Hoerr, I., Jung, G., Rammensee, H.-G., & Pascolo, S.
Affiliations
  • University of Tübingen (DE)
  • CureVac (Germany) (DE)
Corresponding author
Pascolo, Steve
Venue
Genetic Vaccines and Therapy
Published
2006-05-29
Volume
4
Issue
1
Pages
4-4
ISSN
1479-0556 (ISSN-L)
OpenAlex
W2163659675
Cited by
84
References
44
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 7 · 2017: 5 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 5
FWCI
1.22
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Genetic Vaccines and Therapy (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft
  • Eberhard Karls Universität Tübingen
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
ribonuclease activity, skin surface, blood plasma, RNA degradation, RNA therapeutics, 5' cap

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