Genetic Engineering of the Calvin Cycle toward Enhanced Photosynthetic CO2 Fixation in Microalgae
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Photosynthetic microalgae are emerging as potential biomass feedstock for sustainable production of biofuels and value-added bioproducts. CO 2 biomitigation through these organisms is considered as an eco-friendly and promising alternative to the existing carbon sequestration methods. Nonetheless, the inherent relatively low photosynthetic capacity of microalgae has hampered the practical use of this strategy for CO 2 biomitigation applications. Here, we demonstrate the feasibility of improving photosynthetic capacity by the genetic manipulation of the Calvin cycle in the typical green microalga Chlorella vulgaris . Firstly, we fused a plastid transit peptide to upstream of the enhanced green fluorescent protein (EGFP) and confirmed its expression in the chloroplast of C. vulgaris . Then we introduced the cyanobacterial fructose 1,6-bisphosphate aldolase, guided by the plastid transit peptide, into C. vulgaris chloroplast, leading to enhanced photosynthetic capacity (~ 1.2-fold) and cell growth. Molecular and physiochemical analyses suggested a possible role for aldolase overexpression in promoting the regeneration of ribulose 1,5-bisphosphate in the Calvin cycle and energy transfer in photosystems. Our work represents a proof-of-concept effort to enhance photosynthetic capacity by the engineering of the Calvin cycle in green microalgae. Our work also provides insights into targeted genetic engineering toward algal trait improvement for CO 2 biomitigation uses.
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Overexpressing fructose-1,6-bisphosphate aldolase in Chlorella vulgaris facilitates regeneration of ribulose-1,5-bisphosphate in the Calvin cycle, improving energy transfer within photosystems and enhancing carbon fixation efficiency.
“Their research suggests that overexpressing this enzyme facilitates the regeneration of ribulose-1,5-bisphosphate in the Calvin cycle, improving energy transfer within photosystems and ultimately enhancing carbon fixation efficiency.”
From Algal-Based Carbonaceous Materials for Environmental Remediation: Advances in Wastewater Treatment, Carbon Sequestration, and Biofuel Applications (2025) · cites this paper as [105]
Introducing cyanobacterial fructose-1,6-bisphosphate aldolase into Chlorella vulgaris via a plastid transit peptide significantly boosted photosynthetic capacity and cell growth.
“Yang et al., 2017 [105], employed genetic engineering to enhance the photosynthetic performance of Chlorella vulgaris.By introducing cyanobacterial fructose-1,6-bisphosphate aldolase via a plastid transit peptide, they significantly boosted photosynthetic capacity and cell growth.”
From Algal-Based Carbonaceous Materials for Environmental Remediation: Advances in Wastewater Treatment, Carbon Sequestration, and Biofuel Applications (2025) · cites this paper as Yang et al., 2017 [105]
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- Authors
- Yang, B., Liu, J., Ma, X., Guo, B., Liu, B., Wu, T., Jiang, Y., & Chen, F.
- Affiliations
- Peking University (CN)
- Corresponding author
- Liu, Jin
- Chen, Feng
- Venue
- Biotechnology for Biofuels
- Published
- 2017-10-05
- Volume
- 10
- Issue
- 1
- Pages
- 229-229
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC5629779
- OpenAlex
- W2761439032
- Cited by
- 231
- References
- 49
- Citations by year
- 2026: 20 · 2025: 42 · 2024: 29 · 2023: 27 · 2022: 39 · 2021: 32 · 2020: 20 · 2019: 16 · 2018: 5
- FWCI
- 4.09
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (CN) (31471717)
- National Natural Science Foundation of China (31571807)
- National High Technology Research and Development Program of China (2012AA023107)
- Public Science and Technology Research Funds Projects of Ocean (201505032)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- photosynthetic carbon fixation, Chlorella vulgaris, microalgae, fructose-1,6-bisphosphate aldolase, plastid transit peptide, chloroplast targeting, ribulose-1,5-bisphosphate regeneration
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