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Cited by 480Gut microbiota and healthGestational Diabetes Research and ManagementOral microbiology and periodontitis research

Gestational diabetes is associated with change in the gut microbiota composition in third trimester of pregnancy and postpartum

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Crusell, Mie Korslund Wiinblad, Hansen, Tue Haldor, Nielsen, Trine, Allin, Kristine Højgaard, Rühlemann, Malte C., Damm, Peter, Vestergaard, Henrik, Rørbye, Christina +7 more·2018·Microbiome
DOI 10.1186/s40168-018-0472-xPMC PMC5952429
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Abstract

BACKGROUND: Imbalances of gut microbiota composition are linked to a range of metabolic perturbations. In the present study, we examined the gut microbiota of women with gestational diabetes mellitus (GDM) and normoglycaemic pregnant women in late pregnancy and about 8 months postpartum. METHODS: Gut microbiota profiles of women with GDM (n = 50) and healthy (n = 157) pregnant women in the third trimester and 8 months postpartum were assessed by 16S rRNA gene amplicon sequencing of the V1-V2 region. Insulin and glucose homeostasis were evaluated by a 75 g 2-h oral glucose tolerance test during and after pregnancy. RESULTS: Gut microbiota of women with GDM was aberrant at multiple levels, including phylum and genus levels, compared with normoglycaemic pregnant women. Actinobacteria at phylum level and Collinsella, Rothia and Desulfovibrio at genus level had a higher abundance in the GDM cohort. Difference in abundance of 17 species-level operational taxonomic units (OTUs) during pregnancy was associated with GDM. After adjustment for pre-pregnancy body mass index (BMI), 5 of the 17 OTUs showed differential abundance in the GDM cohort compared with the normoglycaemic pregnant women with enrichment of species annotated to Faecalibacterium and Anaerotruncus and depletion of species annotated to Clostridium (sensu stricto) and to Veillonella. OTUs assigned to Akkermansia were associated with lower insulin sensitivity while Christensenella OTUs were associated with higher fasting plasma glucose concentration. OTU richness and Shannon index decreased from late pregnancy to postpartum regardless of metabolic status. About 8 months after delivery, the microbiota of women with previous GDM was still characterised by an aberrant composition. Thirteen OTUs were differentially abundant in women with previous GDM compared with women with previous normoglycaemic pregnancy. CONCLUSION: GDM diagnosed in the third trimester of pregnancy is associated with a disrupted gut microbiota composition compared with normoglycaemic pregnant women, and 8 months after pregnancy, differences in the gut microbiota signatures are still detectable. The gut microbiota composition of women with GDM, both during and after pregnancy, resembles the aberrant microbiota composition reported in non-pregnant individuals with type 2 diabetes and associated intermediary metabolic traits.

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  • In women in the third trimester of pregnancy, GDM was associated with altered intestinal microflora.

    “So far, it was shown that in women in the third trimester of pregnancy, GDM was associated with altered intestinal microflora [87].”

    From Gestational Diabetes Mellitus—Recent Literature Review (2022) · cites this paper as [87]

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Cited by

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Authors
Crusell, M. K. W., Hansen, T. H., Nielsen, T., Allin, K. H., Rühlemann, M. C., Damm, P., Vestergaard, H., Rørbye, C., Jørgensen, N. R., Christiansen, O. B., Heinsen, F.-A., Franke, A., Hansen, T., Lauenborg, J., & Pedersen, O.
Affiliations
  • University of Copenhagen (DK)
  • Christian-Albrechts-Universität zu Kiel (DE)
  • Hvidovre Hospital (DK)
  • University of Southern Denmark (DK)
  • Aalborg University Hospital (DK)
  • Herlev Hospital (DK)
Corresponding author
Pedersen, Oluf
ORCID
Venue
Microbiome
Publisher
Springer Science and Business Media LLC
Published
2018-05-15
Volume
6
Issue
1
Pages
89-89
ISSN
2049-2618 (ISSN-L)
DOI
10.1186/s40168-018-0472-x
PMC
PMC5952429
OpenAlex
W2805293481
Cited by
480
References
10
Citations by year
2026: 41 · 2025: 56 · 2024: 66 · 2023: 56 · 2022: 75 · 2021: 93 · 2020: 50 · 2019: 40 · 2018: 3
FWCI
16.81
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiome (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
  • VBN Forskningsportal (Aalborg Universitet) (acceptedVersion, cc-by)
  • University of Southern Denmark Research Portal (University of Southern Denmark) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Augustinus Fonden (1076001001)
  • Aase og Ejnar Danielsens Fond (10-001605)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Obstetrics and Gynecology › Gestational Diabetes Research and Management
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords
gestational diabetes mellitus, gut microbiota, third trimester, postpartum period, oral glucose tolerance test, insulin sensitivity, fasting blood glucose, Akkermansia, Faecalibacterium, Clostridium, Veillonella, Desulfovibrio, Collinsella, Rothia, microbiome diversity, type 2 diabetes
MeSH terms
Gastrointestinal Microbiome, Faecalibacterium, Adult, Blood Glucose, Clostridium, Desulfovibrio, Female, Glucose, Humans, Pregnancy, Pregnancy Trimester, Third, Surveys and Questionnaires, RNA, Ribosomal, 16S, Body Mass Index, Diabetes, Gestational, Actinobacteria, Gastrointestinal Tract, Postpartum Period, Dysbiosis

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