Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation
Abstract
Molecular markers, especially surface markers associated with type II, cytokine-dependent, alternatively activated macrophages (aaMF), remain scarce. Besides the earlier documented markers, macrophage mannose receptor and arginase 1, we demonstrated recently that murine aaMF are characterized by increased expression of found in inflammatory zone 1 (FIZZ1) and the secretory lectin Ym. We now document that expression of the two members of the mouse macrophage galactose-type C-type lectin gene family (mMGL1 and mMGL2) is induced in diverse populations of aaMF, including peritoneal macrophages elicited during infection with the protozoan Trypanosoma brucei brucei or the Helminth Taenia crassiceps and alveolar macrophages elicited in a mouse model of allergic asthma. In addition, we demonstrate that in vitro, interleukin-4 (IL-4) and IL-13 up-regulate mMGL1 and mMGL2 expression and that in vivo, induction of mMGL1 and mMGL2 is dependent on IL-4 receptor signaling. Moreover, we show that expression of MGL on human monocytes is also up-regulated by IL-4. Hence, macrophage galactose-type C-type lectins represent novel surface markers for murine and human aaMF.
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Human M2a Mp or monocytes do not express arginase.
“Importantly, in contrast to rodent Mp, human M2a Mp or monocytes do not express arginase [36,37].”
From Macrophage phenotypes during tissue repair (2013) · cites this paper as [37]
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- Authors
- Raes, G., Brys, L., Dahal, B. K., Brandt, J., Grooten, J., Brombacher, F., Vanham, G., Noël, W., Bogaert, P., Boonefaes, T., Kindt, A., Van den Bergh, R., Leenen, P. J. M., De Baetselier, P., & Ghassabeh, G. H.
- Affiliations
- Vrije Universiteit Brussel (BE)
- Instituut voor Tropische Geneeskunde (BE)
- Vlaams Instituut voor Biotechnologie (BE)
- University of Cape Town (ZA)
- Laboratory of Cellular and Molecular Immunology, Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel , Brussels, Belgium
- Erasmus MC (NL)
- Corresponding author
- Raes, Geert
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-12-09
- Volume
- 77
- Issue
- 3
- Pages
- 321-327
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2134046076
- Cited by
- 246
- References
- 40
- Citations by year
- 2026: 1 · 2025: 10 · 2024: 7 · 2023: 9 · 2022: 5 · 2021: 15 · 2020: 8 · 2019: 14 · 2018: 7 · 2017: 7 · 2016: 13 · 2015: 15 · 2014: 13 · 2013: 18 · 2012: 22
- FWCI
- 3.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Leukocyte Biology (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Funders
- Institute for Promotion of Innovation by Science and Technology in Flanders
- Generisch Basisonderzoek aan de Universiteiten
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Keywords
- M2 macrophages, cell surface markers, parasitic infection, Trypanosoma brucei brucei, Taenia crassiceps, allergic airway inflammation, allergic asthma, alveolar macrophages, IL-4, IL-13, IL-4 receptor signaling, monocytes, Fizz1
- MeSH terms
- Animals, Asialoglycoproteins, Asthma, Female, Gene Expression Regulation, Humans, Inflammation, Macrophage Activation, Macrophages, Membrane Proteins, Mice, Inbred BALB C, Mice, Inbred C57BL, Parasitic Diseases, RNA, Messenger, Taenia, Trypanosoma brucei brucei, Biomarkers, Bronchial Hyperreactivity, Mice, Knockout, Lectins, C-Type, Mice
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