Cited by 360Sperm and Testicular FunctionHormonal and reproductive studiesAdrenal Hormones and Disorders

Direct Inhibitory Effect of Glucocorticoids upon Testicular Luteinizing Hormone Receptor and Steroidogenesis in Vivo and in Vitro *

BAMBINO, THOMAS H., HSUEH, AARON J. W.·1981·Endocrinology

Abstract

The direct effects of glucocorticoids on testicular LH receptor content and steroidogenesis were studied in vivo and in vitro. Immature hypophysectomized rats were treated with varying doses of dexamethasone, corticosterone, or a synthetic progestin, 17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione (R5020). Some animals were also treated concomitantly with FSH to prevent the hypophysectomy-induced decrease in testis functions. At the end of 5 days of treatment, testicular LH/hCG receptor content was measured by [125I]hCG binding assay while steroidogenic responsiveness was measured by in vitro incubation of testes. Dexamethasone decreased testicular LH receptor in control and FSH-treated hypophysectomized rats in doses as low as 10 microgram/day, whereas corticosterone (10 microgram/day) decreased testicular LH receptor in the FSH-treated rats but had no effect in rats not treated with FSH. In contrast, R5020 had no effect on testicular LH receptor content. In vivo treatment of hypophysectomized rats with FSH increased both basal and hCG-stimulated production of androstanediol in vitro. In contrast, concomitant treatment with dexamethasone, but not R5020, decreased both basal and hCG-stimulated testicular androstanediol production. The direct effect of glucocorticoids on testicular steroidogenic potentials was also studied in primary culture of testicular cells obtained from adult hypophysectomized rats. Treatment of cultured testicular cells wtih hCG increased testosterone…

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Crossref · 10.1210/endo-108-6-2142

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Authors
BAMBINO, T. H., & HSUEH, A. J. W.
Affiliations
University of California San Diego (US)
Venue
Endocrinology
Published
1981-06
Volume
108
Issue
6
Pages
2142-2148
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
OpenAlex
W2050045288
Cited by
360
References
36
Citations by year
2026: 4 · 2025: 3 · 2024: 6 · 2023: 10 · 2022: 7 · 2021: 8 · 2020: 6 · 2019: 6 · 2018: 8 · 2017: 4 · 2016: 7 · 2015: 6 · 2014: 7 · 2013: 8 · 2012: 2
FWCI
11.41
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Keywords
glucocorticoids, luteinizing hormone receptor, testicular steroidogenesis, dexamethasone, corticosterone, R5020, progestins, hypophysectomy, follicle-stimulating hormone, androstanediol, testosterone production, testicular cells
MeSH terms
Androgens, Animals, Corticosterone, Dexamethasone, Follicle Stimulating Hormone, Glucocorticoids, Chorionic Gonadotropin, Humans, Hypophysectomy, Male, Promegestone, Receptors, Cell Surface, Receptors, LH, Testis, Time Factors, Rats

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