The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Bmp signals from niche cells directly repress transcription of a differentiation-promoting gene,bag of marbles, in germline stem cells in the Drosophila ovary
Abstract
The Drosophila ovary is an attractive system to study how niches control stem cell self-renewal and differentiation. The niche for germline stem cells (GSCs) provides a Dpp/Bmp signal, which is essential for GSC maintenance. bam is both necessary and sufficient for the differentiation of immediate GSC daughters, cystoblasts. Here we show that Bmp signals directly repress bam transcription in GSCs in the Drosophila ovary. Similar to dpp, gbb encodes another Bmp niche signal that is essential for maintaining GSCs. The expression of phosphorylated Mad (pMad), a Bmp signaling indicator, is restricted to GSCs and some cystoblasts, which have repressed bam expression. Both Dpp and Gbb signals contribute to pMad production. bam transcription is upregulated in GSCs mutant for dpp and gbb. In marked GSCs mutant for Med and punt, two essential Bmp signal transducers, bam transcription is also elevated. Finally, we show that Med and Mad directly bind to the bam silencer in vitro. This study demonstrates that Bmp signals maintain the undifferentiated or self-renewal state of GSCs, and directly repress bam expression in GSCs by functioning as short-range signals. Thus, niche signals directly repress differentiation-promoting genes in stem cells in order to maintain stem cell self-renewal.
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Citations
Cites
Analysis of genetic mosaics in developing and adult Drosophila tissues
Xu, T., & Rubin, G. M. (1993) · Development · doi:10.1242/dev.117.4.1223 · Cited by 2,041
NEW EMBO MEMBERS REVIEW: Transcriptional control by the TGF-beta/Smad signaling system
Massague, J. (2000) · The EMBO Journal · doi:10.1093/emboj/19.8.1745 · Cited by 1,966
Out of Eden: Stem Cells and Their Niches
Watt, F. M., & Hogan, A. B. L. M. (2000) · Science · doi:10.1126/science.287.5457.1427 · Cited by 1,758
Spradling, A., Drummond-Barbosa, D., & Kai, T. (2001) · Nature · doi:10.1038/35102160 · Cited by 1,437
A Niche Maintaining Germ Line Stem Cells in the Drosophila Ovary
Xie, T., & Spradling, A. C. (2000) · Science · doi:10.1126/science.290.5490.328 · Cited by 777
Self-citationLin, H., & Spradling, A. C. (1997) · Development · doi:10.1242/dev.124.12.2463 · Cited by 698
Xie, T., & Spradling, A. C. (1998) · Cell · doi:10.1016/s0092-8674(00)81424-5 · Cited by 668
Self-citationThe product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A
Lasko, P. F., & Ashburner, M. (1988) · Nature · doi:10.1038/335611a0 · Cited by 630
Hay, B., Jan, L. Y., & Jan, Y. N. (1988) · Cell · doi:10.1016/0092-8674(88)90216-4 · Cited by 616
Cox, D. N., Chao, A., & Lin, H. (2000) · Development · doi:10.1242/dev.127.3.503 · Cited by 546
Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic
Kim, J., Johnson, K., Chen, H. J., Carroll, S., & Laughon, A. (1997) · Nature · doi:10.1038/40906 · Cited by 527
Lin, H., Yue, L., & Spradling, A. C. (1994) · Development · doi:10.1242/dev.120.4.947 · Cited by 511
Germline Stem Cells Anchored by Adherens Junctions in the Drosophila Ovary Niches
Song, X., Zhu, C.-H., Doan, C., & Xie, T. (2002) · Science · doi:10.1126/science.1069871 · Cited by 483
Self-citationForbes, A., & Lehmann, R. (1998) · Development · doi:10.1242/dev.125.4.679 · Cited by 475
Identification and behavior of epithelial stem cells in the Drosophila ovary
Margolis, J., & Spradling, A. (1995) · Development · doi:10.1242/dev.121.11.3797 · Cited by 454
Chen, D., & McKearin, D. M. (2003) · Development · doi:10.1242/dev.00325 · Cited by 447
Daughters against dpp modulates dpp organizing activity in Drosophila wing development
Tsuneizumi, K., Nakayama, T., Kamoshida, Y., Kornberg, T. B., Christian, J. L., & Tabata, T. (1997) · Nature · doi:10.1038/39362 · Cited by 429
Chen, D., & McKearin, D. (2003) · Current Biology · doi:10.1016/j.cub.2003.09.033 · Cited by 423
Hedgehog Creates a Gradient of DPP Activity in Drosophila Wing Imaginal Discs
Tanimoto, H., Itoh, S., ten Dijke, P., & Tabata, T. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)80403-7 · Cited by 415
bag-of-marbles: a Drosophila gene required to initiate both male and female gametogenesis.
McKearin, D. M., & Spradling, A. C. (1990) · Genes & Development · doi:10.1101/gad.4.12b.2242 · Cited by 399
The stem-cell niche theory: lessons from flies
Lin, H. (2002) · Nature Reviews Genetics · doi:10.1038/nrg952 · Cited by 354
McKearin, D., & Ohlstein, B. (1995) · Development · doi:10.1242/dev.121.9.2937 · Cited by 345
Liang, L., Diehl-Jones, W., & Lasko, P. (1994) · Development · doi:10.1242/dev.120.5.1201 · Cited by 343
Nakamura, A., Amikura, R., Hanyu, K., & Kobayashi, S. (2001) · Development · doi:10.1242/dev.128.17.3233 · Cited by 334
Ruberte, E., Marty, T., Nellen, D., Affolter, M., & Basler, K. (1995) · Cell · doi:10.1016/0092-8674(95)90292-9 · Cited by 307
GERMLINE CYST FORMATION IN DROSOPHILA
de Cuevas, M., Lilly, M., & Spradling, A. (1997) · Annual Review of Genetics · doi:10.1146/annurev.genet.31.1.405 · Cited by 305
An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells
Kai, T., & Spradling, A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0830856100 · Cited by 279
Transducing the Dpp Morphogen Gradient in the Wing of Drosophila
Campbell, G., & Tomlinson, A. (1999) · Cell · doi:10.1016/s0092-8674(00)80659-5 · Cited by 278
Letsou, A., Arora, K., Wrana, J. L., Simin, K., Twombly, V., Jamal, J., Staehling-Hampton, K., Hoffmann, F. M., Gelbart, W. M., Massagué, J., et al. (1995) · Cell · doi:10.1016/0092-8674(95)90293-7 · Cited by 274
The Drosophila Gene brinker Reveals a Novel Mechanism of Dpp Target Gene Regulation
Jaźwińska, A., Kirov, N., Wieschaus, E., Roth, S., & Rushlow, C. (1999) · Cell · doi:10.1016/s0092-8674(00)80660-1 · Cited by 269
Ectopic expression of the Drosophila Bam protein eliminates oogenic germline stem cells
Ohlstein, B., & McKearin, D. (1997) · Development · doi:10.1242/dev.124.18.3651 · Cited by 250
brinker is a target of Dpp in Drosophila that negatively regulates Dpp-dependent genes
Minami, M., Kinoshita, N., Kamoshida, Y., Tanimoto, H., & Tabata, T. (1999) · Nature · doi:10.1038/18451 · Cited by 238
Haerry, T. E., Khalsa, O., O'Connor, M. B., & Wharton, K. A. (1998) · Development · doi:10.1242/dev.125.20.3977 · Cited by 217
Conversion of an Extracellular Dpp/BMP Morphogen Gradient into an Inverse Transcriptional Gradient
Müller, B., Hartmann, B., Pyrowolakis, G., Affolter, M., & Basler, K. (2003) · Cell · doi:10.1016/s0092-8674(03)00241-1 · Cited by 182
Khalsa, O., Yoon, J., Torres‐Schumann, S., & Wharton, K. A. (1998) · Development · doi:10.1242/dev.125.14.2723 · Cited by 153
Medea is a Drosophila Smad4 homolog that is differentially required to potentiate DPP responses
Wisotzkey, R. G., Mehra, A., Sutherland, D. J., Dobens, L. L., Liu, X., Dohrmann, C. E., Attisano, L., & Raftery, L. A. (1998) · Development · doi:10.1242/dev.125.8.1433 · Cited by 152
King, F. J., Szakmary, A., Cox, D. N., & Lin, H. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00197-6 · Cited by 149
Developmental territories created by mutual antagonism between Wingless and Decapentaplegic
Theisen, H., Haerry, T. E., O’Connor, M. B., & Marsh, J. L. (1996) · Development · doi:10.1242/dev.122.12.3939 · Cited by 139
Schnurri mediates Dpp-dependent repression of brinker transcription
Marty, T., Muller, B. H., Basler, K., & Affolter, M. (2000) · Nature Cell Biology · doi:10.1038/35036383 · Cited by 123
Morphogen gradients: new insights from DPP
Podos, S. D., & Ferguson, E. L. (1999) · Trends in Genetics · doi:10.1016/s0168-9525(99)01854-5 · Cited by 115
Das, P. K., Maduzia, L. L., Wang, H., Finelli, A. L., Cho, S., Smith, M. M., & Padgett, R. W. (1998) · Development · doi:10.1242/dev.125.8.1519 · Cited by 111
King, F. J., & Lin, H. (1999) · Development · doi:10.1242/dev.126.9.1833 · Cited by 108
Newfeld, S. J., Mehra, A., Singer, M. A., Wrana, J. L., Attisano, L., & Gelbart, W. M. (1997) · Development · doi:10.1242/dev.124.16.3167 · Cited by 91
Cited by
Stem cell division is regulated by the microRNA pathway
Hatfield, S. D., Shcherbata, H. R., Fischer, K. A., Nakahara, K., Carthew, R. W., & Ruohola-Baker, H. (2005) · Nature · doi:10.1038/nature03816 · Cited by 674
Beyond tumorigenesis: cancer stem cells in metastasis
Li, F., Tiede, B., Massagué, J., & Kang, Y. (2007) · Cell Research · doi:10.1038/sj.cr.7310118 · Cited by 620
Notch Signaling in the Regulation of Stem Cell Self-Renewal and Differentiation
Liu, J., Sato, C., Cerletti, M., & Wagers, A. (2010) · Current Topics in Developmental Biology · doi:10.1016/s0070-2153(10)92012-7 · Cited by 311
Niu, W., & Spradling, A. C. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2005570117 · Cited by 267
The stem cell niche: lessons from the Drosophila testis
de Cuevas, M., & Matunis, E. L. (2011) · Development · doi:10.1242/dev.056242 · Cited by 263
Metadata
- Authors
- Song, X., Wong, M. D., Kawase, E., Xi, R., Ding, B. C., McCarthy, J. J., & Xie, T.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2004-03-15
- Volume
- 131
- Issue
- 6
- Pages
- 1353-1364
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2064695569
- Cited by
- 416
- References
- 43
- FWCI
- 6.01
- Type
- journal article
- Language
- en
- Open access
- closed
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