The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Clonal analysis by distinct viral vectors identifies bona fide neural stem cells in the adult zebrafish telencephalon and characterizes their division properties and fate
Abstract
Neurogenesis is widespread in the zebrafish adult brain through the maintenance of active germinal niches. To characterize which progenitor properties correlate with this extensive neurogenic potential, we set up a method that allows progenitor cell transduction and tracing in the adult zebrafish brain using GFP-encoding retro- and lentiviruses. The telencephalic germinal zone of the zebrafish comprises quiescent radial glial progenitors and actively dividing neuroblasts. Making use of the power of clonal viral vector-based analysis, we demonstrate that these progenitors follow different division modes and fates: neuroblasts primarily undergo a limited amplification phase followed by symmetric neurogenic divisions; by contrast, radial glia are capable at the single cell level of both self-renewing and generating different cell types, and hence exhibit bona fide neural stem cell (NSC) properties in vivo. We also show that radial glial cells predominantly undergo symmetric gliogenic divisions, which amplify this NSC pool and may account for its long-lasting maintenance. We further demonstrate that blocking Notch signaling results in a significant increase in proliferating cells and in the numbers of clones, but does not affect clone composition, demonstrating that Notch primarily controls proliferation rather than cell fate. Finally, through long-term tracing, we illustrate the functional integration of newborn neurons in forebrain adult circuitries. These results characterize fundamental aspects of adult progenitor cells and neurogenesis, and open the way to using virus-based technologies for stable genetic manipulations and clonal analyses in the zebrafish adult brain.
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Cites
Functional neurogenesis in the adult hippocampus
van Praag, H., Schinder, A. F., Christie, B. R., Toni, N., Palmer, T. D., & Gage, F. H. (2002) · Nature · doi:10.1038/4151030a · Cited by 2,853
The Glial Nature of Embryonic and Adult Neural Stem Cells
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The cell biology of neurogenesis
Götz, M., & Huttner, W. B. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1739 · Cited by 2,130
Self-citationNew neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory?
Deng, W., Aimone, J. B., & Gage, F. H. (2010) · Nature reviews. Neuroscience · doi:10.1038/nrn2822 · Cited by 2,120
Astrocytes give rise to new neurons in the adult mammalian hippocampus
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Neurons born in the adult dentate gyrus form functional synapses with target cells
Toni, N., Laplagne, D. A., Zhao, C., Lombardi, G., Ribak, C. E., Gage, F. H., & Schinder, A. F. (2008) · Nature Neuroscience · doi:10.1038/nn.2156 · Cited by 741
Imayoshi, I., Sakamoto, M., Yamaguchi, M., Mori, K., & Kageyama, R. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.4987-09.2010 · Cited by 716
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Synapse formation on neurons born in the adult hippocampus
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Chapouton, P., Skupien, P., Hesl, B., Coolen, M., Moore, J. C., Madelaine, R., Kremmer, E., Faus-Kessler, T., Blader, P., Lawson, N. D., et al. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.6170-09.2010 · Cited by 259
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Cited by
Acute Inflammation Initiates the Regenerative Response in the Adult Zebrafish Brain
Kyritsis, N., Kizil, C., Zocher, S., Kroehne, V., Kaslin, J., Freudenreich, D., Iltzsche, A., & Brand, M. (2012) · Science · doi:10.1126/science.1228773 · Cited by 517
Neurogenesis in zebrafish – from embryo to adult
Schmidt, R., Strähle, U., & Scholpp, S. (2013) · Neural Development · doi:10.1186/1749-8104-8-3 · Cited by 375
Glial Cells as Progenitors and Stem Cells: New Roles in the Healthy and Diseased Brain
Dimou, L., & Götz, M. (2014) · Physiological Reviews · doi:10.1152/physrev.00036.2013 · Cited by 264
Self-citationCosacak, M. I., Bhattarai, P., Reinhardt, S., Petzold, A., Dahl, A., Zhang, Y., & Kizil, C. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.03.090 · Cited by 116
Spatial Regionalization and Heterochrony in the Formation of Adult Pallial Neural Stem Cells
Dirian, L., Galant, S., Coolen, M., Chen, W., Bedu, S., Houart, C., Bally-Cuif, L., & Foucher, I. (2014) · Developmental Cell · doi:10.1016/j.devcel.2014.05.012 · Cited by 109
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Metadata
- Authors
- Rothenaigner, I., Krecsmarik, M., Hayes, J. A., Bahn, B., Lepier, A., Fortin, G., Götz, M., Jagasia, R., & Bally-Cuif, L.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2011-04-15
- Volume
- 138
- Issue
- 8
- Pages
- 1459-1469
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2156911768
- Cited by
- 197
- References
- 11
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- closed
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